FastQCFastQC Report
Sat 18 Jun 2016
SRR3137833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4277035
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51550.043.851851
GTATCAA101500.033.1863171
TATGCCG18800.032.4820543
AATCTCG22200.029.99245536
CTCGTAT19600.029.39497839
TCGTATG19400.029.34235640
GCGAATC27650.029.19702333
AATGCGA7250.029.02614420
CTCGATA7650.028.86333865
TCTCGTA20600.028.4704638
ATGCCGT20350.028.14308444
ATGCGAC8150.027.51429421
AATCGCG3150.027.38023436
ATCTCGT24300.026.69065937
ATCAACG125250.026.4442733
CCGTCTT22300.026.30098747
TGCCGTC22300.026.14627545
TCAACGC127350.026.0620884
CAACGCA128000.026.0375565
TATCAAC129450.025.8261622
Produced by FastQC (version 0.11.5)