FastQCFastQC Report
Sat 18 Jun 2016
SRR3137832_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137832_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5229636
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394220.7538191950644366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.040.272721
GTATCAA110450.032.0603371
ATGCGAC9950.026.69106921
CGACGAG9600.026.23160724
CTCGATA9400.025.33807865
CAACGCA142250.025.2220545
ATCAACG138850.025.1600693
TCAACGC140450.024.9756344
GCTCGAT9750.024.78135164
TATCAAC145950.023.9308412
AATGCGA10550.023.865420
AACGCAG154250.023.4192096
CAATGCG10650.023.31656519
ATCGTCA11050.022.7811510
CGCACTA10800.021.7303558
GTACTAG18000.021.6245291
GATAACG9500.021.4335162
CGCAGAG169900.021.0040848
TGGTCGT11450.020.49714351
GTCGTCT11450.020.49694653
Produced by FastQC (version 0.11.5)