FastQCFastQC Report
Sat 18 Jun 2016
SRR3137832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5229636
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG119470.2284480220038259RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52950.042.433541
TATGCCG40550.034.8832543
CTCGTAT40300.034.24323339
TCGTATG42750.033.3298840
TCTCGTA41300.032.913238
GTATCAA108050.032.773051
ATCTCGT42600.031.98948937
ATGCCGT44250.031.96646144
CTCGATA9950.031.5534265
TGCCGTC46150.030.72515345
CCGTCTT47000.030.02267847
CGTATGC47900.029.8184241
GCTCGAT10650.029.80371964
GCCGTCT49550.028.40798846
CATCTCG48500.028.31138836
CGTCTTC51250.027.53299348
TGGTCGT11250.026.98671351
ATCAACG131700.025.987623
TCAACGC133300.025.804614
CAACGCA134550.025.4879555
Produced by FastQC (version 0.11.5)