FastQCFastQC Report
Sat 18 Jun 2016
SRR3137830_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137830_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165350
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG324420.7788541179012568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.038.367611
GTATCAA88350.033.3316881
ATAACGC2500.028.939563
ATCAACG107800.027.0053963
TCAACGC109350.026.6881834
CAACGCA112700.026.3591735
TATCAAC113900.025.7026122
AACGCAG120050.024.8350526
GTGGTAT22900.022.259941
GTACATG122800.021.5687851
ACGCAGA138600.021.2652997
CGCAGAG138350.021.1303278
TACATGG123950.020.6732352
TGGTATC23050.020.3212322
ACATGGG120850.020.269153
AGAGTAC142450.019.48738711
TAACGCA3750.019.2948954
CGTACGA2153.1377567E-919.2636960
GAGTACT94550.018.92961112
CAGAGTA150750.018.91519710
Produced by FastQC (version 0.11.5)