FastQCFastQC Report
Sat 18 Jun 2016
SRR3137830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165350
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG84660.20324822643955487TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG43170.10364075047715078TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42300.038.9207761
GTATCAA91950.034.946321
TATGCCG34400.031.7922943
CTCGTAT33350.031.34421239
TCTCGTA33700.031.12104838
TCGTATG35950.030.80468240
ATGCCGT36900.029.73183444
TGCCGTC37150.029.43924145
GATCTCG36200.029.0667636
ATCTCGT36450.028.86774637
CCGTCTT38200.028.81068247
GCCGTCT38650.028.2967146
ATCAACG112400.028.1164573
TCAACGC113400.027.8076674
CGTATGC39850.027.61677241
CAACGCA115300.027.43925
CGTCTTC40600.026.93763448
TATCAAC118500.026.8738822
AACGCAG123350.025.5916166
ATGCGAC4800.025.1551521
Produced by FastQC (version 0.11.5)