FastQCFastQC Report
Sat 18 Jun 2016
SRR3137829_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137829_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502845
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG452931.0058751744730277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60550.042.1510961
GTATCAA125750.035.278711
TCAACGC164400.026.933614
ATCAACG164350.026.8729253
CAACGCA169000.026.471465
GCTCGAT6650.025.9526564
TATCAAC172450.025.6226942
CTCGATA6800.025.38016365
AACGCAG183150.024.560496
GTCGTAT5200.024.5481527
GTGGTAT25400.023.4594171
GATAACG12350.022.077562
GACTGAC16600.021.8085047
CGCAGAG205050.021.5233238
TGGTATC27550.021.2514532
GTACATG217800.021.1435721
ACGCAGA215200.020.6676967
GAGTACT110600.020.58111212
ACATGGG213550.020.4557913
TACATGG221300.020.4180242
Produced by FastQC (version 0.11.5)