FastQCFastQC Report
Sat 18 Jun 2016
SRR3137829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502845
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG118340.26281162242981937TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG60480.1343150830197353TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60750.043.6896481
GTATCAA123100.035.018931
CTCGTAT41200.032.8260439
TCTCGTA41700.032.10185638
TATGCCG45050.032.01116643
TCGTATG46550.031.0544640
ATCTCGT43750.030.67651437
GATCTCG43900.030.5713636
ATGCCGT47950.030.29098944
CCGTCTT48900.029.49118647
TGCCGTC49350.029.29218145
GTACTAG8950.027.7656571
ATGCGAC6700.027.28958721
TCAACGC159550.026.7036654
ATCAACG159600.026.6304513
CAACGCA161100.026.4681535
CGTATGC55500.026.1087441
TATCAAC168200.025.5366172
CGACGAG7050.025.44601224
CGTCTTC58050.025.19929948
Produced by FastQC (version 0.11.5)