FastQCFastQC Report
Sat 18 Jun 2016
SRR3137828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810362
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314330.5409817839232736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59350.041.086411
GTATCAA126850.033.5322071
ATCAACG154300.026.812983
TCAACGC155400.026.7818224
CAACGCA159750.026.4891385
CGTACGA3700.026.11605560
TATCAAC166250.025.293332
AACGCAG173350.024.6156886
CGCAGAG191950.021.697718
CTCGATA9850.021.37789265
ACGCAGA201250.020.8997127
GTACATG194050.020.8372361
GCTCGAT10200.020.64344464
ACATGGG184250.020.4726773
AGAGTAC197650.020.23834411
TACATGG201750.019.6990032
TCGATAA10700.019.68032866
TGGTCGT10950.019.54255551
CAGAGTA207750.019.50240510
ATGCGAC8850.019.48584421
Produced by FastQC (version 0.11.5)