FastQCFastQC Report
Sat 18 Jun 2016
SRR3137828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810362
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG87150.14999065462702668RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.040.421021
TATGCCG31100.035.16730543
CTCGTAT30600.033.37359639
TCTCGTA31100.032.9471338
GTATCAA117600.032.3722531
TATCTCG32600.031.74864436
TCGTATG34000.031.65981740
ATGCCGT35400.030.60318244
TGCCGTC35250.030.53765745
CCGTCTT36200.029.54563547
ATCTCGT36400.029.09768937
GCCGTCT38300.027.65539246
ATCAACG143300.025.8570883
TCAACGC144050.025.7703654
CAACGCA144100.025.713545
CGTATGC42850.025.28244241
CTCGATA11200.025.25863665
TGGTCGT11900.024.93377151
TCCTATC42650.024.59162733
TATCAAC153100.024.4500542
Produced by FastQC (version 0.11.5)