FastQCFastQC Report
Sat 18 Jun 2016
SRR3137827_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137827_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4776782
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG343660.7194383164230648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52350.038.229181
GTATCAA101850.031.8585051
TGCGACG5100.031.1114422
CGACGAG5250.029.56708124
CTCGATA6600.029.28625965
ATGCGAC6500.026.5323321
ATCAACG120250.026.3303743
TCAACGC122600.025.8864944
CAACGCA124400.025.8228595
TATCAAC125750.025.0918182
CAATGCG6600.025.08353219
AACGCAG130850.024.8694576
GCTCGAT7500.024.85122164
GCGACGA6650.023.34145223
TCGTCTA9100.023.13609154
AATGCGA7350.022.99444420
TGGTCGT8200.022.73073251
CATTGCG9200.022.49905429
TTGCGCC9200.022.12546331
TCGATAA8700.021.82042566
Produced by FastQC (version 0.11.5)