FastQCFastQC Report
Sat 18 Jun 2016
SRR3137827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4776782
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47050.039.757661
GTATCAA95200.031.79391
CGACGAG5300.029.29203424
TGCGACG5350.027.72857922
CTCGATA7300.026.93769365
ATGCGAC6250.026.49534421
ATCAACG110650.026.348423
TCAACGC113400.025.7700394
CAACGCA114450.025.5939085
TATCAAC116500.025.4103492
CGCACTA5800.024.98214358
AACGCAG121200.024.5378076
GCTCGAT8100.024.27717864
CAATGCG6150.023.55965219
ATCGGGG10300.023.44651437
CGGTCGA2400.022.99870913
TATGCCG8650.022.33517643
TGCGCGA2800.022.17732612
AGCATCG26500.021.87184334
TTGCGCC9850.021.71633531
Produced by FastQC (version 0.11.5)