FastQCFastQC Report
Sat 18 Jun 2016
SRR3137825_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137825_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4354765
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198900.4567410640987516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.039.825791
GTATCAA86850.033.2739871
CGACGAG5000.030.35813724
ATCAACG104450.026.8160423
TCAACGC105900.026.4865844
CAACGCA108100.026.3673955
CAATGCG6100.026.00940319
TATCAAC109050.025.8693732
GCTCGAT6450.025.15015864
AACGCAG113600.025.1246176
TGCGACG6400.023.71483422
CGTACGA3350.023.693360
ACCGTCG3500.023.654623
ATGCGAC7200.022.51504321
CTCGATA7450.022.23860465
TAACTCG4000.021.56415630
GTCGTCT7550.021.48218253
GTACATG139200.021.4284781
TACCTGG23850.020.9414772
ACATGGG130150.020.8325843
Produced by FastQC (version 0.11.5)