FastQCFastQC Report
Sat 18 Jun 2016
SRR3137825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4354765
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG50790.11663086297423626TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.037.712551
TATGCCG20350.034.7552243
CTCGTAT19750.033.36505539
GTATCAA81700.033.2055471
CGACGAG5200.033.17281324
ATCTCGT19900.032.93943837
TCGTATG22600.031.14206140
GCTCGAT8200.031.13425664
TCTCGTA21950.030.49213438
GATCTCG21900.030.24633236
ATGCCGT23700.030.13370944
CTCGATA8800.029.01146565
TGCCGTC24500.029.0089345
GTCGTCT8850.028.4583853
CCGTCTT25450.027.65495747
CAATGCG6350.027.16388919
ATGCGAC6750.027.08836221
CGCACTA6250.027.04809458
TGCGACG6750.026.57726122
GCCGTCT26650.026.40970646
Produced by FastQC (version 0.11.5)