FastQCFastQC Report
Sat 18 Jun 2016
SRR3137824_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137824_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4288806
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG326740.7618437392598313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51650.038.9456831
GTATCAA104650.034.4277951
ATCAACG134800.026.298963
CAACGCA139350.025.9202925
TCAACGC137150.025.9028154
GTACTAG12400.025.833191
TATCAAC139200.025.5359572
TGGTCGT7950.025.17852451
CTCGATA8000.025.02584365
GCTCGAT8150.024.98761464
ATGCGAC8150.024.54772621
ATCGTCA8150.024.53940610
TATATCG3250.024.4289866
AACGCAG153900.023.585576
AATGCGA8900.022.86559720
CGCACTA8800.022.74491158
TACCTGG26200.022.6119942
GTACATG174700.021.7272831
CAATGCG9350.021.39596419
CATACCG13800.021.0038334
Produced by FastQC (version 0.11.5)