FastQCFastQC Report
Sat 18 Jun 2016
SRR3137824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4288806
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG51550.12019662348914827RNA PCR Primer, Index 46 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45090.1051341562197031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.039.481831
TATGCCG22200.036.83281743
AATCGCG4750.035.58949336
CTCGTAT21950.035.3657239
GTATCAA106550.034.5298961
ATCTCGT25050.033.60519837
TCTCGTA23000.033.600838
AATCTCG26250.033.38287436
GCGAATC33200.033.25380333
ATGCCGT23850.032.4040444
TGGTCGT7700.031.81318951
TCGTATG24950.031.80473540
CGAATCT29650.030.95147334
CCGTCTT25400.030.56246647
CGAATCG6500.030.25421534
CTCGATA8100.030.24110865
TGCCGTC26350.029.06778345
GCTCGAT8600.028.88407364
GGCGAAT42950.028.51639632
TAAGGCG44050.028.19524829
Produced by FastQC (version 0.11.5)