FastQCFastQC Report
Sat 18 Jun 2016
SRR3137823_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137823_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612300
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226760.6277440965589791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.035.0127871
GCTCGAT7250.029.99637464
CGACGAG7050.029.35598224
GTATCAA55650.029.149261
TGGTCGT8250.028.45136551
CTCGATA7900.027.9664465
ATGCGAC8050.027.84862321
GTCGTCT8950.026.6092153
CGCACTA7400.026.11534958
TCGATAA8550.025.83998166
GCTCGTC9400.023.8653239
CAACGCA64400.023.7579925
CAATGCG9150.023.74584619
ATCAACG64000.023.7383673
TCAACGC64550.023.6464484
TCGTCTA9950.023.58803454
ATCGTCA9150.023.36179410
CGTACGA3400.023.34665560
ACGAGTC9350.023.24307426
TGCGACG8950.023.12334622
Produced by FastQC (version 0.11.5)