FastQCFastQC Report
Sat 18 Jun 2016
SRR3137823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612300
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG61010.16889516374608973RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30800.032.9462971
TATGCCG23250.031.30913743
GCTCGAT9000.030.28365164
CTCGTAT22450.030.27339
TCGTATG23750.029.9232840
GTATCAA55700.029.805741
TCTCGTA23700.028.82188838
TGGTCGT9800.028.51560451
ATGCCGT25450.028.46709344
CTCGATA9750.027.60028865
TATCTCG25300.027.4082436
TGCCGTC26400.026.7893145
TCGATAA10200.026.7208766
CCGTCTT26100.026.56850647
ATCTCGT26650.026.53763637
CGTATGC27200.026.0013941
CAACGCA62400.025.7650495
GTCGTCT11000.025.7184553
CGTACGA5000.025.5295660
CAATGCG7850.025.49000519
Produced by FastQC (version 0.11.5)