FastQCFastQC Report
Sat 18 Jun 2016
SRR3137822_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137822_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3929455
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239980.6107208251525975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41950.041.6157881
GTATCAA88450.034.0248071
ATCAACG107700.027.5332873
TCAACGC109550.027.389694
CAACGCA112000.027.0749555
TATCAAC113600.026.218562
AACGCAG119950.025.4283496
GCTCGAT6550.022.6635764
CGCAGAG134050.022.426598
ACGCAGA137500.022.0377147
AGAGTAC139450.020.67156611
CAGAGTA146250.020.18042210
TACCTGG20550.020.1066842
GTACATG118100.020.088681
GTACTTT93200.019.90707214
GCAGAGT149200.019.7798989
GTGGTAT21000.019.6763271
CTCGATA7550.019.66252965
GAGTACT96500.019.58267812
GCTCGTC7600.019.52543639
Produced by FastQC (version 0.11.5)