FastQCFastQC Report
Sat 18 Jun 2016
SRR3137822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3929455
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43550.040.976841
GTATCAA87200.033.369311
TATGCCG5550.027.97306443
ATGCGAC5250.027.59904121
ATCAACG105400.026.8424033
CGCACTA4900.026.75450958
TCAACGC106500.026.7267994
CGACGAG5250.026.28446424
CAACGCA108300.026.2825895
TATCAAC111050.025.6948552
AGCATCG20300.025.49269534
CATCGCG9200.025.12476536
GCTCGAT4950.025.09067364
AACGCAG117000.024.5337286
AATGCGA5900.023.38901520
GCATCGC10550.023.21808635
CAATGCG5950.023.19246919
ATCGGGG6950.022.83431637
ATCGCGT6150.022.43881437
CTCGATA5550.022.37816865
Produced by FastQC (version 0.11.5)