FastQCFastQC Report
Sat 18 Jun 2016
SRR3137821_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137821_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5048771
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370650.7341390607734041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.038.617711
CAATGCG6800.034.99477819
GTATCAA109750.034.2057271
ATGCGAC8150.030.04650721
GCTCGAT8350.028.93648164
ATCAACG136750.026.9821683
TCAACGC138500.026.7463114
CAACGCA140150.026.6812695
CTCGATA9350.026.21187865
TATCAAC143000.025.8661732
TGGTCGT10200.024.70070551
ATCGTCA9450.024.08251610
AATGCGA10350.023.99161720
GTCGTCT10700.023.86925753
AACGCAG159200.023.7507066
CGCACTA9900.023.704758
GCTCGTC11350.022.80388639
GTACTAG14750.022.6493911
CGTCATT11450.022.29424726
CGCAGAG172100.021.3563618
Produced by FastQC (version 0.11.5)