FastQCFastQC Report
Sat 18 Jun 2016
SRR3137821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5048771
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAGGTGGTCTCATGGATCCCCGCAGACTCCATACCGATAAAGGAAGGCTGGAAGAGCGTCTCCGGGCAACG50930.10087603497960197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.041.061771
GTATCAA102800.034.7823031
CAATGCG7700.032.256919
ATCAACG128350.027.3376943
CTCGATA9550.027.09319765
CAACGCA129950.027.027655
TCAACGC131900.026.6801244
ATGCGAC9700.026.6742321
TATCAAC136650.026.0811962
AATGCGA10000.025.87272320
CGCACTA9350.025.82788358
GTACTAG15650.025.8025841
GCTCGAT10400.024.8788564
TATGCCG9200.024.37502743
AGCATCG29900.024.00121734
ATCGTCA10150.023.79148110
AACGCAG148950.023.7650836
TGGTCGT12000.023.57572751
GTCGTCT11700.023.29559153
ATCGGGG12050.023.19112837
Produced by FastQC (version 0.11.5)