FastQCFastQC Report
Sat 18 Jun 2016
SRR3137820_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137820_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5542842
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG321260.5795943669330643No Hit
GTTGTAGGTGGTCTCATGGATCCCCGCAGACTCCATACCGATAAAGGAAGGCTGGAAGAGCGTCTCCGGGCAACG56870.10260079576506059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56450.039.4844251
GTATCAA110950.033.036941
AATGCGA10600.029.61006220
GTACTAG15000.028.2487581
CGCACTA10000.027.60715758
ATGCGAC11200.027.40839621
ATCAACG139050.025.8441123
TCAACGC140850.025.6400834
CAACGCA143350.025.462885
TATCAAC150000.024.0903032
CTCGATA10250.023.90916665
TGGTCGT10900.023.43127351
CAATGCG13800.023.24214619
GCTCGAT10800.022.3693464
ATCGTCA14050.022.33360710
AACGCAG166500.022.1124536
GTACATG208750.021.6517431
TAACGCC5700.021.1596374
ACATGGG197500.020.9693833
CCGATAA22350.020.84527438
Produced by FastQC (version 0.11.5)