FastQCFastQC Report
Sat 18 Jun 2016
SRR3137820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5542842
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG73980.1334694367979459RNA PCR Primer, Index 17 (96% over 28bp)
GTTGTAGGTGGTCTCATGGATCCCCGCAGACTCCATACCGATAAAGGAAGGCTGGAAGAGCGTCTCCGGGCAACG60150.10851833770473703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56900.039.3073921
TATGCCG29150.037.39935343
CTCGTAT24950.034.8460439
TCGTATG32150.033.80282240
AATCTCG26600.033.3327436
ATCTCGT26600.033.2030437
TCTCGTA26500.032.67768538
ATGCCGT32900.032.61219844
CCGTCTT33500.031.92540247
GTATCAA109100.031.9211061
TGCCGTC33950.031.5022445
GCTCGAT9400.028.99444664
CTCGATA10150.027.53179765
TGGTCGT10700.026.43951451
GCCGTCT40350.025.90708746
AATGCGA9050.025.54030820
CGTATGC44450.025.1473841
ATCAACG137600.024.4713953
GTCGTCT11850.024.45571753
TCAACGC138050.024.3414234
Produced by FastQC (version 0.11.5)