FastQCFastQC Report
Sat 18 Jun 2016
SRR3137817_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137817_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3878075
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274870.7087794846670061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42550.040.245211
GTATCAA84850.033.947811
GACTGAC16400.026.907317
ATCAACG109150.026.2617283
TCAACGC109600.026.2232064
CAACGCA112050.025.8992145
AACGCAG121800.023.9139826
ACTGACC19800.023.8545278
ATGCGAC6450.023.52724821
CGCACTA6050.022.81608458
GTCGTAT5600.022.79078127
TATCAAC126100.022.7575552
GTACATG131750.021.9154681
GATAACG17050.021.65968162
CGACGAG6700.021.62348224
GTAGTAT12150.021.57659327
CTCGATA6250.021.54022665
GTGGTAT19900.021.4696981
TACCTGG24900.021.1683862
CGCAGAG134700.021.1667088
Produced by FastQC (version 0.11.5)