FastQCFastQC Report
Sat 18 Jun 2016
SRR3137817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3878075
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42150.10868794440540733RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.041.628521
TATGCCG18350.034.59399843
CTCGTAT17700.034.5004439
GTATCAA78800.032.6716961
TCTCGTA19000.032.50304438
TCGTATG19600.031.860140
AATCTCG22700.031.61273636
GCGAATC27400.031.22707633
CGAATCT24900.030.75982534
ATCTCGT22200.030.45984837
TGCCGTC22950.028.11110745
ATGCCGT22350.028.09395644
CCGTCTT22950.027.9607847
GGCGAAT35000.027.20641132
AAGGCGA36600.026.77117730
TAAGGCG36900.026.64702429
AGGCGAA38000.025.87566231
GACTGAC18300.025.6376867
CTCGATA6300.024.64253465
TCAACGC104150.024.4461524
Produced by FastQC (version 0.11.5)