FastQCFastQC Report
Sat 18 Jun 2016
SRR1931153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873414
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77630.41437717450600886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59270.3163742771218748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34670.18506320546339464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.056.114061
GTATCAA67250.044.889581
ATCAACG82300.035.9325183
CAACGCA84600.035.164885
TATCAAC86050.034.692732
AACGCAG89400.034.0581366
TCAACGC88300.033.8200764
ACGCAGA97600.030.2745937
CGCAGAG96850.029.8703658
GTACTAG7100.027.7148631
GCAGAGT106500.026.1369239
GTACATG80350.024.549531
GAGTACT59250.024.36211412-13
GTGGTAT15900.024.147941
TACATGG80650.023.6257212
ACATGGG79350.022.8788173
CAGAGTA102700.022.6540510-11
GTACTTT66250.022.0636414-15
CTGTGCG7350.022.0110029
GCATCGT8100.021.734688
Produced by FastQC (version 0.11.5)