FastQCFastQC Report
Sat 18 Jun 2016
SRR1931153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873414
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89820.4794455470066948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.40546296760886813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47670.2544552352016159No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.10088533554249088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.055.0663381
GTATCAA76750.042.14011
ATCAACG95400.032.903743
CAACGCA96900.032.4915435
TATCAAC99650.032.119952
TCAACGC98900.031.8825024
AACGCAG103000.030.797816
GTGGTAT18000.030.6514131
ACGCAGA114900.027.1948387
CGCAGAG115600.026.8247668
TGGTATC20350.026.1364022
TATCGGC2800.025.4404872
GTACATG88550.024.2243981
GCAGAGT124550.024.0202459
GGCGTCG4350.024.0167398
GTACTAG6150.023.9746131
TACATGG88300.023.7175642
ACATGGG87450.023.4593583
GAGTACT70700.022.93780712-13
GTACTTT75600.021.1998414-15
Produced by FastQC (version 0.11.5)