##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931153_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873414 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30610212158124 31.0 31.0 34.0 30.0 34.0 2 31.314014414325932 31.0 31.0 34.0 28.0 34.0 3 31.45661183272891 31.0 31.0 34.0 28.0 34.0 4 35.123189001470045 35.0 35.0 37.0 32.0 37.0 5 35.015682064935994 35.0 35.0 37.0 32.0 37.0 6 35.1280848760605 37.0 35.0 37.0 32.0 37.0 7 35.0130264853364 36.0 35.0 37.0 32.0 37.0 8 35.04876017794252 36.0 35.0 37.0 32.0 37.0 9 36.44384423304192 38.0 35.0 39.0 32.0 39.0 10-11 36.33357576061671 38.0 35.0 39.0 32.0 39.0 12-13 36.242970053602676 38.0 35.0 39.0 32.0 39.0 14-15 37.1620504063704 39.0 36.0 40.5 31.5 41.0 16-17 37.20052081387243 39.0 36.0 40.0 31.5 41.0 18-19 37.27537292878136 39.0 36.0 40.0 31.5 41.0 20-21 37.2519832775884 39.0 36.0 40.0 31.0 41.0 22-23 37.128879414801 39.0 36.0 40.0 31.0 41.0 24-25 36.98051178223287 39.0 36.0 40.0 31.0 41.0 26-27 36.70971258888852 39.0 35.5 40.0 30.0 41.0 28-29 36.43172785086479 38.0 35.0 40.0 30.0 41.0 30-31 36.217353185147545 38.0 35.0 40.0 30.0 41.0 32-33 36.02355325624769 38.0 34.5 40.0 30.0 41.0 34-35 35.75866279423555 38.0 34.0 40.0 29.0 41.0 36-37 36.00313144878815 38.0 35.0 40.0 30.0 41.0 38-39 35.968115163012556 38.0 35.0 40.0 29.5 41.0 40-41 35.794750119300915 38.0 35.0 40.0 29.0 41.0 42-43 35.60803031257373 38.0 34.0 40.0 28.0 41.0 44-45 35.323189642011855 38.0 34.0 40.0 27.0 41.0 46-47 35.03407895958928 38.0 34.0 40.0 26.5 41.0 48-49 34.70471396071557 38.0 33.0 40.0 25.0 41.0 50-51 34.30313267649329 38.0 33.0 40.0 24.0 41.0 52-53 34.157627465151855 38.0 33.0 40.0 24.0 41.0 54-55 33.9251107870444 37.0 33.0 40.0 23.0 41.0 56-57 33.66610530293892 37.0 32.5 40.0 23.0 41.0 58-59 33.38057551614325 37.0 32.0 40.0 22.0 41.0 60-61 33.079211268838606 36.0 31.5 39.5 22.0 41.0 62-63 32.78033926297124 36.0 31.5 39.0 20.5 41.0 64-65 32.51929045048239 35.5 31.0 39.0 20.0 40.5 66-67 32.25624661713855 35.0 31.0 39.0 19.5 40.0 68-69 31.908355547679264 35.0 31.0 38.5 18.5 40.0 70-71 31.078007584015065 35.0 29.5 37.5 11.5 40.0 72-73 31.055640931475907 34.5 30.0 37.0 14.5 39.5 74-75 30.746844797786288 34.0 30.0 37.0 12.5 39.0 76-77 30.035070198044853 34.0 29.0 36.0 10.5 39.0 78-79 30.061636402845288 34.0 29.0 36.0 7.5 38.5 80-81 29.811053509795485 34.0 29.0 35.5 7.0 37.5 82-83 29.466792444168775 34.0 29.0 35.0 4.5 37.0 84-85 29.115403215733416 34.0 29.0 35.0 2.0 36.5 86-87 28.803275463939098 34.0 29.0 35.0 2.0 36.0 88-89 28.492974056989006 34.0 29.0 35.0 2.0 36.0 90-91 28.176914712925175 33.0 27.5 35.0 2.0 35.5 92-93 27.833219993018094 33.0 27.0 35.0 2.0 35.0 94-95 27.45306990339562 33.0 26.5 35.0 2.0 35.0 96-97 27.08607894464331 33.0 26.0 35.0 2.0 35.0 98-99 26.606431893857952 33.0 25.0 35.0 2.0 35.0 100-101 25.915133814522576 32.5 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8120789048630748 1101 2 0.749914925751046 1101 3 0.5347430966625488 1101 4 0.8010627533706867 1101 5 0.6991412064528149 1101 6 0.7192907176234371 1101 7 0.8458986189551041 1101 8 0.6638070828671871 1101 9 1.1055928145634084 1101 10-11 0.9202563450360017 1101 12-13 0.8905113549981252 1101 14-15 1.3116213347515497 1101 16-17 1.1264951817719577 1101 18-19 0.8394082036260002 1101 20-21 0.8425599740271679 1101 22-23 0.7128269366940714 1101 24-25 0.6132966663925501 1101 26-27 0.7430228043206384 1101 28-29 0.779430142043374 1101 30-31 0.9658334791304028 1101 32-33 1.056571839015497 1101 34-35 1.0059874522258951 1101 36-37 1.0101933641195089 1101 38-39 0.9701734830663185 1101 40-41 0.9197391028095012 1101 42-43 0.854511670734091 1101 44-45 0.8510302629412436 1101 46-47 0.8551502674201927 1101 48-49 0.8963787158273107 1101 50-51 0.6915999192744309 1101 52-53 0.6338645268375558 1101 54-55 0.6402604926652202 1101 56-57 0.43849492339104046 1101 58-59 0.5948470618425858 1101 60-61 0.8060739945623112 1101 62-63 0.6574227927555611 1101 64-65 0.5600021832895905 1101 66-67 0.35909634142022284 1101 68-69 0.36860750503687 1101 70-71 0.6895423384975707 1101 72-73 0.19533190984645898 1101 74-75 0.011011731646703993 1101 76-77 0.10809161738432493 1101 78-79 0.21650919308516237 1101 80-81 0.05800345284952968 1101 82-83 -0.11175422403814395 1101 84-85 0.06789469020399608 1101 86-87 -0.10449202129559154 1101 88-89 -0.22742346924748702 1101 90-91 -0.09963463140537598 1101 92-93 -0.23742284123116164 1101 94-95 -0.09394342716504411 1101 96-97 -0.30091319245198633 1101 98-99 -0.26490362970017145 1101 100-101 -0.6782303073994491 1102 1 0.8318018898981343 1102 2 0.8187011213928663 1102 3 0.5512968946420926 1102 4 0.8287702971409558 1102 5 0.746437286906442 1102 6 0.6684988143467265 1102 7 0.9058412940879563 1102 8 0.7428567492314855 1102 9 1.1800469650246228 1102 10-11 1.0693544310315843 1102 12-13 1.0854323469171447 1102 14-15 1.5004418259063712 1102 16-17 1.2482138508740945 1102 18-19 1.0814331300024733 1102 20-21 1.068834189020265 1102 22-23 0.7047500948634919 1102 24-25 0.5549975664426654 1102 26-27 0.8598393688877408 1102 28-29 1.063195689963905 1102 30-31 1.301393204269253 1102 32-33 1.3947454786889395 1102 34-35 1.324240037850771 1102 36-37 1.4641043366126922 1102 38-39 1.2417719936138454 1102 40-41 1.3493867039874772 1102 42-43 1.597367724844787 1102 44-45 1.5306816333120778 1102 46-47 1.3876217396006467 1102 48-49 1.2789558496667937 1102 50-51 1.2421930079830048 1102 52-53 1.3147000036404535 1102 54-55 1.110411964514313 1102 56-57 1.0788967773368867 1102 58-59 1.1232399359971055 1102 60-61 1.5212360491451946 1102 62-63 1.3620902546805027 1102 64-65 1.334836193835045 1102 66-67 1.1281863091542768 1102 68-69 1.072114428340008 1102 70-71 1.262953913647408 1102 72-73 0.7300366909226241 1102 74-75 0.6213491365367148 1102 76-77 0.7484237205525162 1102 78-79 0.8019224581588276 1102 80-81 0.7593699302281642 1102 82-83 0.7203019327934186 1102 84-85 0.6592747946644799 1102 86-87 0.6226221024084886 1102 88-89 0.6451441380606049 1102 90-91 0.6935663245613384 1102 92-93 0.618163309677378 1102 94-95 0.46545582148156583 1102 96-97 0.4476355854597145 1102 98-99 0.3595964137905163 1102 100-101 0.29922954655468104 1103 1 0.6743266538922867 1103 2 0.5056923792043264 1103 3 0.3407439429069541 1103 4 0.6968541623184592 1103 5 0.5905700485072956 1103 6 0.5890333177372327 1103 7 0.6238008406908051 1103 8 0.5616371640699427 1103 9 0.844268478121279 1103 10-11 0.703479713573806 1103 12-13 0.7753541492040767 1103 14-15 1.1448585424334254 1103 16-17 0.9864741387347635 1103 18-19 0.820341935347507 1103 20-21 0.8156812672013558 1103 22-23 0.5399864331163755 1103 24-25 0.2587168192016023 1103 26-27 0.6900785325627794 1103 28-29 0.6951113261824915 1103 30-31 1.061806796211819 1103 32-33 1.2218246569897033 1103 34-35 1.093893425033329 1103 36-37 1.2091695706241268 1103 38-39 1.0531206340871861 1103 40-41 1.1537345300744306 1103 42-43 1.2862419603187902 1103 44-45 1.2062175494136582 1103 46-47 1.0256812562872568 1103 48-49 0.8743896350676721 1103 50-51 0.7856717674564848 1103 52-53 0.3948070372795982 1103 54-55 0.6006011846978012 1103 56-57 0.7184006664473799 1103 58-59 0.8705182234588875 1103 60-61 1.0599028653930347 1103 62-63 0.8754597367972323 1103 64-65 0.8576082653680857 1103 66-67 0.805591989914813 1103 68-69 0.7207687794901219 1103 70-71 1.0102803163864031 1103 72-73 0.3643285071704625 1103 74-75 0.13927835140176015 1103 76-77 0.40548503221319976 1103 78-79 0.4135809809616475 1103 80-81 0.35634913844515737 1103 82-83 0.2840139557956789 1103 84-85 0.3996231525956091 1103 86-87 0.3587082279237137 1103 88-89 0.11111882560215491 1103 90-91 0.29091416726842567 1103 92-93 -0.05616000171738378 1103 94-95 -0.11471902228920783 1103 96-97 -0.261366957420222 1103 98-99 -0.23706019769731768 1103 100-101 -0.21839950875711267 1104 1 0.4065902464280846 1104 2 0.32074406592626303 1104 3 0.1198767690108582 1104 4 0.5649274593530507 1104 5 0.2787080920138081 1104 6 0.3135856477005916 1104 7 0.4432457363427531 1104 8 0.34017661621017936 1104 9 0.5846419479863982 1104 10-11 0.4035971538628047 1104 12-13 0.4357139907977512 1104 14-15 0.7812383115162618 1104 16-17 0.6605975598694585 1104 18-19 0.5300622715223682 1104 20-21 0.43097977345185257 1104 22-23 0.1016216307669211 1104 24-25 0.16213801887933954 1104 26-27 -0.009842036868313642 1104 28-29 0.42489047996267715 1104 30-31 0.6014141428084869 1104 32-33 0.7794418698701193 1104 34-35 0.5206772017051051 1104 36-37 0.6120244417159455 1104 38-39 0.18624586149577027 1104 40-41 0.4731396292245762 1104 42-43 0.7671788674321292 1104 44-45 0.5612880298305285 1104 46-47 0.46706953694842923 1104 48-49 0.3021766896717182 1104 50-51 0.18300345261904027 1104 52-53 0.1694119960861613 1104 54-55 0.40015700700723045 1104 56-57 0.4621841279958403 1104 58-59 0.2579613713134421 1104 60-61 0.6216802746245236 1104 62-63 -0.3585030039309913 1104 64-65 -1.1793630096793457 1104 66-67 -0.5336201530537146 1104 68-69 -0.09493857490341284 1104 70-71 0.4458968048080578 1104 72-73 0.00665655379572172 1104 74-75 -0.2656870337634061 1104 76-77 -0.37943233725427206 1104 78-79 -0.43488092485221586 1104 80-81 -0.5419713679612705 1104 82-83 -0.6541858941146579 1104 84-85 -0.7338085078768835 1104 86-87 -0.4614432586786741 1104 88-89 -0.4472252013490099 1104 90-91 -0.2770170806199239 1104 92-93 -0.3378242914229226 1104 94-95 -0.33946432467764964 1104 96-97 -0.4204356055736618 1104 98-99 -0.5039495894118495 1104 100-101 -0.8153687208838356 1105 1 0.02443423264495692 1105 2 -1.359504420449536 1105 3 -0.13499880417814225 1105 4 0.48606247805095393 1105 5 0.5707385962428333 1105 6 0.037520602067083075 1105 7 -0.0444755769803038 1105 8 -0.10021413374455079 1105 9 0.5679369315299709 1105 10-11 0.8816081611178177 1105 12-13 0.944848036926409 1105 14-15 1.2701576072646859 1105 16-17 0.7744421716712395 1105 18-19 0.6687060693039442 1105 20-21 0.9720273242363007 1105 22-23 0.18911385844437234 1105 24-25 -0.2194918830659276 1105 26-27 0.7663470493345059 1105 28-29 0.5359309137690786 1105 30-31 0.8963297458330786 1105 32-33 1.1397436322719656 1105 34-35 0.6215616318593078 1105 36-37 0.6601754204270662 1105 38-39 0.5601240826564933 1105 40-41 0.9580483432170297 1105 42-43 1.1272619283911993 1105 44-45 1.306725312411281 1105 46-47 1.2512715552828766 1105 48-49 1.2291593442538797 1105 50-51 0.803833403699322 1105 52-53 1.0871646139816846 1105 54-55 0.2917798679564214 1105 56-57 0.4251765386632016 1105 58-59 -0.11778524653902878 1105 60-61 0.45430978272990075 1105 62-63 0.9042928033837967 1105 64-65 1.2513143316101392 1105 66-67 0.8916500578342514 1105 68-69 0.934715479178017 1105 70-71 0.12705574516347795 1105 72-73 -0.46676887813013224 1105 74-75 -0.30854409521129256 1105 76-77 0.10675002454402005 1105 78-79 -0.43411331697997824 1105 80-81 -0.35495845989059305 1105 82-83 -0.21893766734392983 1105 84-85 -0.051403779772037694 1105 86-87 -0.40766606821339835 1105 88-89 -0.5968129991508526 1105 90-91 -0.5643774795759313 1105 92-93 -0.02612452405439214 1105 94-95 -0.40502068367220545 1105 96-97 -0.48556260310805044 1105 98-99 -1.065071133973376 1105 100-101 -0.6231459822656191 1106 1 0.2985335963265072 1106 2 0.2844588710535483 1106 3 -0.385406846510584 1106 4 0.3064332381406061 1106 5 -0.07269169040251455 1106 6 0.20651756110157038 1106 7 0.3675080973927791 1106 8 0.28317538940856934 1106 9 0.7730897145486608 1106 10-11 -0.09537288475740979 1106 12-13 0.09166095165939936 1106 14-15 -0.15369357339586287 1106 16-17 -0.49657513426877387 1106 18-19 0.11923516864374761 1106 20-21 0.292372024125342 1106 22-23 0.11885935073209453 1106 24-25 0.11705305975194591 1106 26-27 0.3742933172749474 1106 28-29 0.3463126795520779 1106 30-31 0.022567745848242282 1106 32-33 0.030398472430910317 1106 34-35 -1.2362620618222024 1106 36-37 -1.2321729113126878 1106 38-39 -1.0876279827022088 1106 40-41 0.2601141655238308 1106 42-43 0.5206151583247802 1106 44-45 0.41797392054370164 1106 46-47 0.027224213662172758 1106 48-49 -0.09689991509696227 1106 50-51 0.2231942923816561 1106 52-53 -0.28399473761242433 1106 54-55 0.32943144911519084 1106 56-57 0.0847768636922055 1106 58-59 0.3758881656220794 1106 60-61 0.5952451037002575 1106 62-63 0.4110533532694802 1106 64-65 0.40043571651242615 1106 66-67 0.0924673647295684 1106 68-69 0.2738932749317975 1106 70-71 0.45923261904643553 1106 72-73 -0.23490460021685067 1106 74-75 -0.09097134370085413 1106 76-77 -0.015895320429820003 1106 78-79 0.09063031667457011 1106 80-81 0.029494544225375563 1106 82-83 0.08032889605597404 1106 84-85 0.18725455399914281 1106 86-87 0.10441497718417381 1106 88-89 0.2669586016647507 1106 90-91 0.4808963239236945 1106 92-93 -0.1474537937759237 1106 94-95 0.05834808685108328 1106 96-97 0.16240644327405107 1106 98-99 0.31583093951947916 1106 100-101 0.20053589840704333 1107 1 0.4587224105295391 1107 2 0.49982269436434024 1107 3 0.10594011443347284 1107 4 -1.040497680335271 1107 5 0.0366551397634538 1107 6 0.256177204451042 1107 7 0.5353197395785898 1107 8 0.3497551153350571 1107 9 0.9357729873637695 1107 10-11 0.7679074634325787 1107 12-13 0.7483641294335257 1107 14-15 1.048336222657042 1107 16-17 0.8817867300396145 1107 18-19 0.7523797745146155 1107 20-21 0.17003145662850727 1107 22-23 0.28639876097629013 1107 24-25 0.23700208965876612 1107 26-27 0.7271988261018052 1107 28-29 0.7444220353899169 1107 30-31 1.0001276268668775 1107 32-33 1.077077242743222 1107 34-35 1.069869595683322 1107 36-37 1.0673661292832293 1107 38-39 0.9305216474493463 1107 40-41 0.2452950396285729 1107 42-43 0.0776118862035915 1107 44-45 -0.1575053878270367 1107 46-47 0.32694741115977166 1107 48-49 0.7538806165826983 1107 50-51 -1.124747820965574 1107 52-53 0.3847318507742159 1107 54-55 0.8535329836225927 1107 56-57 0.8231522928912156 1107 58-59 0.9765414221966822 1107 60-61 1.4247281565946537 1107 62-63 1.2527359668562568 1107 64-65 0.9337607761298798 1107 66-67 0.8543466525494559 1107 68-69 1.0061156283769321 1107 70-71 1.3651125954529988 1107 72-73 0.8103971635525511 1107 74-75 0.7729428778825991 1107 76-77 0.8030729405285193 1107 78-79 0.9039020575311127 1107 80-81 0.8406973556874071 1107 82-83 0.8566953602834957 1107 84-85 0.8351083587741464 1107 86-87 0.7577974924204902 1107 88-89 0.8748672064627065 1107 90-91 1.1179270741228642 1107 92-93 0.9062427890459723 1107 94-95 0.9382694465161805 1107 96-97 1.1265712450184715 1107 98-99 0.9714597018916891 1107 100-101 0.7436027580381115 1108 1 0.5762556522013007 1108 2 0.49690373257037734 1108 3 0.2338957934801904 1108 4 0.6917242325211745 1108 5 0.634610311705444 1108 6 0.4865323978253784 1108 7 0.6560250170079343 1108 8 0.511571995033556 1108 9 0.6861128910521401 1108 10-11 0.6188257296319506 1108 12-13 0.6149790809858899 1108 14-15 0.9839621477643732 1108 16-17 0.7487431400825812 1108 18-19 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60-61 2.1649572167304143 2311 62-63 2.217585253659884 2311 64-65 -0.5227776048993888 2311 66-67 1.4532648966211035 2311 68-69 1.9942985475480057 2311 70-71 2.446752648083006 2311 72-73 1.470659266958549 2311 74-75 1.8328147417622702 2311 76-77 1.9635533388419688 2311 78-79 1.6256844504021402 2311 80-81 1.5358365560701692 2311 82-83 1.8914491056858438 2311 84-85 2.058048013688772 2311 86-87 1.6514422697895874 2311 88-89 1.8073752056254158 2311 90-91 1.79456652868463 2311 92-93 1.8264956223942583 2311 94-95 1.962438879248456 2311 96-97 1.6953147036470995 2311 98-99 2.4314212760072955 2311 100-101 2.5370352211821583 2312 1 0.024445648663913744 2312 2 -0.016691585479506443 2312 3 -0.24680969722326296 2312 4 0.28576421953459885 2312 5 -1.4851241904194126 2312 6 -0.036533911225966165 2312 7 0.34219940535490423 2312 8 -2.2797051956025456 2312 9 -4.5435434978084785 2312 10-11 -0.4095375745785077 2312 12-13 -5.556306783517638 2312 14-15 -2.2912680852670775 2312 16-17 -3.546685634866904 2312 18-19 -2.5339619144108028 2312 20-21 -3.271115553850237 2312 22-23 -1.712325641461895 2312 24-25 -3.4984783568859896 2312 26-27 -3.900403735366588 2312 28-29 -5.496914174458723 2312 30-31 -6.158637183762533 2312 32-33 -1.6443031421619239 2312 34-35 0.5974062370092597 2312 36-37 0.6178934904228797 2312 38-39 0.8191679730434842 2312 40-41 -3.2689805236890095 2312 42-43 -5.001449479456291 2312 44-45 -4.322305743967021 2312 46-47 -0.5725372131810289 2312 48-49 -1.7162498539330144 2312 50-51 -6.178417090193712 2312 52-53 -1.3519128995853507 2312 54-55 0.34715927634227484 2312 56-57 0.8261957488188969 2312 58-59 0.7857668177175441 2312 60-61 1.3600579285280787 2312 62-63 1.264635836728182 2312 64-65 1.1654871989776368 2312 66-67 1.0856535835815713 2312 68-69 1.1103764666756817 2312 70-71 1.5818143624552619 2312 72-73 0.910283301488306 2312 74-75 0.8726791973127774 2312 76-77 1.0811703311182015 2312 78-79 0.8418172688180121 2312 80-81 0.5225641798775307 2312 82-83 -0.40719923597703556 2312 84-85 -0.01717915577964746 2312 86-87 0.806920840136403 2312 88-89 0.46934703661132815 2312 90-91 0.714423033303742 2312 92-93 0.8633690132771896 2312 94-95 1.059913511991148 2312 96-97 1.2945991198215694 2312 98-99 1.1114757966797164 2312 100-101 1.1788041141096137 2313 1 -0.7494118782178063 2313 2 -0.49115395107090265 2313 3 -0.34761614883616687 2313 4 -2.4820449202503454 2313 5 -1.223363437731237 2313 6 -2.7670446639141417 2313 7 -4.362437691419288 2313 8 -3.6691541203337295 2313 9 -1.5109494117869744 2313 10-11 -2.0790604240408754 2313 12-13 -5.74901511685097 2313 14-15 -1.7781632465573978 2313 16-17 -7.099104989705609 2313 18-19 -5.222806000432307 2313 20-21 -4.385866897936253 2313 22-23 -1.6614183833973826 2313 24-25 0.39819503021077907 2313 26-27 -1.1492276600977718 2313 28-29 -3.6770217013404434 2313 30-31 0.033567117312735206 2313 32-33 -6.255526260441492 2313 34-35 -2.267345107076764 2313 36-37 -2.6245460257061524 2313 38-39 -0.4083185860962999 2313 40-41 0.2318595300744306 2313 42-43 -1.206590608488547 2313 44-45 -4.218811120311109 2313 46-47 -2.103619202428341 2313 48-49 -1.383924585115814 2313 50-51 0.19607887754822428 2313 52-53 -2.2961333296928785 2313 54-55 -4.188963035485685 2313 56-57 -7.996048874837022 2313 58-59 -3.254051730669559 2313 60-61 -8.021662501579442 2313 62-63 -3.096587281551386 2313 64-65 -3.316703661237419 2313 66-67 -5.3271488357732615 2313 68-69 -7.995974339775934 2313 70-71 -4.861566013888826 2313 72-73 -1.1078618598020142 2313 74-75 0.027753122043961298 2313 76-77 -4.358849830172119 2313 78-79 -2.034526817203492 2313 80-81 -0.2591898523805334 2313 82-83 1.1054015704745765 2313 84-85 1.251974070026801 2313 86-87 1.2476130444374753 2313 88-89 -3.749402963388672 2313 90-91 -0.2928014290618499 2313 92-93 1.4945854111266534 2313 94-95 0.5733543722062038 2313 96-97 1.8283692811118897 2313 98-99 0.9900040224861684 2313 100-101 0.9894559958300455 2314 1 -28.780661878217806 2314 2 -14.73790719782415 2314 3 -5.333005759225777 2314 4 -3.4215741410295664 2314 5 -1.3154900611078588 2314 6 -0.8149342743037522 2314 7 0.03731880208720639 2314 8 -4.322157367086977 2314 9 -1.6671994117869744 2314 10-11 -0.4717146448200964 2314 12-13 0.41230793509708263 2314 14-15 -1.4035690907132405 2314 16-17 -0.6072218728224925 2314 18-19 -2.5336826238089287 2314 20-21 -1.9006802096245679 2314 22-23 -5.208293383397383 2314 24-25 -0.8487611386203895 2314 26-27 -3.851946815941929 2314 28-29 -2.0747489740677096 2314 30-31 -3.450199116453497 2314 32-33 -0.9247632734285034 2314 34-35 -0.24806750967417202 2314 36-37 -4.75543076596589 2314 38-39 -8.48745819648591 2314 40-41 -8.099512223172326 2314 42-43 -4.92960197212491 2314 44-45 -5.139265665765656 2314 46-47 -5.238156540090678 2314 48-49 -6.24796679290802 2314 50-51 -2.5013643042699556 2314 52-53 -3.16382813488768 2314 54-55 -6.040626996524644 2314 56-57 -2.3657729008110486 2314 58-59 -2.717322834565664 2314 60-61 -1.5378962678132098 2314 62-63 0.2146951859810784 2314 64-65 0.07493594915219148 2314 66-67 0.4741904499410232 2314 68-69 1.0228974134708224 2314 70-71 1.3370947003968894 2314 72-73 1.0203848934447421 2314 74-75 1.0758456545114967 2314 76-77 0.015135559438270718 2314 78-79 -1.2374488951255707 2314 80-81 -1.0284674497831325 2314 82-83 -1.456895507447502 2314 84-85 -0.07797722867449508 2314 86-87 0.7819068756063068 2314 88-89 0.6576587249230172 2314 90-91 1.4732294150939929 2314 92-93 1.0357785929448333 2314 94-95 0.7957569696088029 2314 96-97 0.6593351902027962 2314 98-99 1.1919114900186365 2314 100-101 1.6629543724534237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 13.0 4 49.0 5 94.0 6 205.0 7 563.0 8 1359.0 9 2272.0 10 3600.0 11 5292.0 12 7048.0 13 8518.0 14 10679.0 15 13387.0 16 16338.0 17 18844.0 18 17225.0 19 13785.0 20 13133.0 21 13446.0 22 14147.0 23 16252.0 24 19008.0 25 23225.0 26 28977.0 27 36329.0 28 45195.0 29 56029.0 30 68842.0 31 83314.0 32 100423.0 33 121797.0 34 149955.0 35 184962.0 36 230707.0 37 276410.0 38 224249.0 39 47715.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.15486347370126 21.567941810373636 14.963329392786928 22.31386532313817 2 15.427342808370174 21.58310976644778 40.040482242579586 22.949065182602457 3 18.264998860335897 27.361760345988138 30.55516855018179 23.818072243494175 4 12.603908058616076 17.86810262136819 38.74045791339968 30.787531406616058 5 12.01463429996792 39.07816080542029 35.51054309074323 13.396661803868568 6 29.52518770544044 39.040009309207676 17.701373001376098 13.733429983975778 7 26.331446225981015 33.32167903090294 22.900544140270114 17.446330602845926 8 25.73552882598294 35.545960476435 20.05408308040828 18.66442761717378 9 25.902816996136462 15.989258113796524 21.221417156058404 36.8865077340086 10-11 24.231269756711544 27.292605905582 28.216961120179523 20.259163217526932 12-13 25.20876325254322 24.978008064421424 28.326894108830192 21.486334574205166 14-15 22.557854270332133 26.212652408917624 26.487951942282912 24.741541378467332 16-17 21.25830168878849 29.134937605889565 27.33613605962163 22.27062464570031 18-19 21.019112700129284 27.975930573808032 29.999562296427808 21.00539442963488 20-21 22.334999097903612 27.030784439531253 29.79704432656103 20.83717213600411 22-23 22.1460659523202 26.83731412277265 29.632291634417168 21.384328290489982 24-25 21.71995174886346 27.07642583896944 30.00720990926085 21.196412502906252 26-27 21.68192094467088 27.22260035393128 29.72267375596892 21.37280494542892 28-29 21.38550205212014 27.199606684757672 30.083651554083644 21.331239709038538 30-31 21.780761006148307 27.03639232566904 29.836411755976687 21.346434912205968 32-33 21.82156411638794 27.293329205344325 29.47360133234404 21.4115053459237 34-35 21.93133863950397 27.12603360764533 29.771263138537385 21.171364614313315 36-37 21.898028996167636 26.852134811548396 29.69719793390095 21.55263825838302 38-39 21.657241272736442 27.071177938039277 29.91755882964445 21.354021959579832 40-41 21.635623061136958 26.809936907464905 30.182087314260826 21.372352717137314 42-43 21.690715387044143 26.965740348550977 29.70861027194364 21.63493399246124 44-45 21.685645968387433 27.321413653941057 29.430386081353134 21.56255429631838 46-47 21.834952765270693 27.13261098526159 29.15199077330934 21.880445476158375 48-49 22.0157279796273 27.222404629770512 29.034378928991856 21.727488461610328 50-51 21.88232176596153 27.31462968142246 28.725377295917482 22.07767125669853 52-53 21.760465719583944 27.53109049739458 28.60875562212488 22.099688160896594 54-55 21.9683994319491 27.51714819010535 28.49754060872437 22.016911769221178 56-57 22.1192751147646 27.54857478381552 28.258060211380375 22.0740898900395 58-59 21.742596613345114 27.4715654704792 28.723472115278113 22.062365800897574 60-61 21.879639947420046 27.736539731895615 28.363522323126507 22.020297997557833 62-63 21.944770881336908 27.471366005610832 28.37811472174187 22.205748391310394 64-65 21.750359744683372 27.45315711827359 28.41968317911001 22.376799957933027 66-67 21.979963086384 27.44248428902587 28.213426360272088 22.36412626431804 68-69 21.884573439944653 27.42329637808692 28.291875450730696 22.400254731237727 70-71 22.060107052241214 27.105318928025596 28.33433246623189 22.500241553501297 72-73 22.03478814643915 27.342931137149982 28.25038881701672 22.371891899394146 74-75 21.947917806537365 27.569800121171035 28.127106814669702 22.355175257621898 76-77 21.84784035989909 27.66855057130992 27.92084397789277 22.56276509089822 78-79 21.790378421427405 27.592459541777735 27.873924290092845 22.74323774670201 80-81 21.618441262779186 27.658130972572703 27.980751651736206 22.7426761129119 82-83 21.996679309418063 27.40015442465606 28.167580377019284 22.43558588890659 84-85 21.960293859389253 27.52481700511313 27.71962188796597 22.795267247531644 86-87 21.43773175091259 27.786889380633717 27.928211897834633 22.847166970619064 88-89 21.92006777256156 27.35938167679972 27.807755677139756 22.91279487349896 90-91 21.830375623104864 27.308120267078685 27.99660827045013 22.864895839366323 92-93 21.83260237351975 27.49063702400424 27.695458796499935 22.981301805976077 94-95 21.48856567278426 27.293057413443194 27.888153800001497 23.330223113771048 96-97 21.5708165067659 27.341976613128576 27.815793891485622 23.271412988619897 98-99 21.346172528982404 27.42217534315018 27.800902967794006 23.430749160073407 100-101 21.47712662873633 27.32294853087776 27.59377402952417 23.606150810861738 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1165.0 1 762.0 2 375.0 3 428.5 4 384.0 5 374.0 6 645.5 7 1276.0 8 2045.0 9 2456.5 10 2299.5 11 1975.5 12 2000.0 13 2155.5 14 2283.0 15 2457.5 16 2614.5 17 2808.0 18 3047.5 19 3283.5 20 3613.5 21 4101.0 22 4697.5 23 5744.5 24 7045.5 25 8510.5 26 11012.5 27 13981.5 28 16324.0 29 19653.5 30 24129.5 31 28135.0 32 34049.0 33 40499.5 34 45943.0 35 51344.5 36 57813.0 37 64193.0 38 64853.5 39 62462.0 40 60399.5 41 58240.0 42 56656.0 43 57477.5 44 59974.5 45 62793.5 46 63940.0 47 64316.5 48 65565.0 49 65382.0 50 64112.5 51 63563.5 52 62567.0 53 59906.0 54 57124.5 55 53506.0 56 49211.0 57 43783.5 58 38554.5 59 35121.0 60 30586.0 61 24268.5 62 18587.5 63 14421.5 64 10778.5 65 7665.0 66 5484.0 67 3925.0 68 2903.5 69 2291.5 70 1854.5 71 1447.5 72 1174.0 73 912.5 74 659.0 75 461.5 76 348.0 77 256.5 78 173.0 79 129.5 80 116.0 81 94.0 82 79.0 83 79.5 84 57.0 85 32.0 86 27.0 87 21.0 88 9.5 89 5.0 90 5.5 91 2.5 92 1.5 93 1.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1662739789496609 2 0.0 3 0.0029358166427708985 4 1.60135453242049E-4 5 3.736493908981143E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.015880099113169857 26-27 0.009020963865968762 28-29 0.005738187074506756 30-31 0.0027756811895288495 32-33 0.001281083625936392 34-35 0.0032293983070479882 36-37 0.0015212868057994655 38-39 9.34123477245286E-4 40-41 0.0029091274005638903 42-43 0.001574665290213482 44-45 7.206095395892205E-4 46-47 0.03130648110882058 48-49 0.01702773652807121 50-51 0.0875674036811938 52-53 6.40541812968196E-4 54-55 0.03707135742553435 56-57 7.472987817962286E-4 58-59 0.0016814222590415147 60-61 0.00408345405767225 62-63 0.003869940120016184 64-65 0.011236170969150439 66-67 0.006485485856302984 68-69 0.0035763584557390944 70-71 0.0065655535829240086 72-73 0.00552467313685069 74-75 0.0015479760480064737 76-77 0.0 78-79 0.0 80-81 3.469601486911062E-4 82-83 4.5371711751913886E-4 84-85 5.3378484414016335E-5 86-87 0.0021084501343536454 88-89 0.0047773743550544616 90-91 0.0017881792278695472 92-93 0.004163521784293274 94-95 0.0010675696882803266 96-97 6.672310551752041E-4 98-99 0.001921625438904588 100-101 0.0036030476979461027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1873414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.68765664552585 #Duplication Level Percentage of deduplicated Percentage of total 1 78.50253085564945 41.361143915272564 2 12.535564201624327 13.209390050262556 3 3.7667612306286955 5.953854671551293 4 1.6730498076512965 3.5259629526557825 5 0.8482158690228305 2.234525323418061 6 0.5029119547373692 1.5898351436479645 7 0.31498595827931786 1.161711041258784 8 0.23466691768898396 0.9891239988208851 9 0.18150834019765624 0.8606924195970068 >10 1.201512486291685 12.599920728678779 >50 0.14648830936217408 5.421839987835978 >100 0.08718355363313193 8.048894166756844 >500 0.0032856997212752383 1.120924926437652 >1k 0.0011294592791883632 1.0253325352689584 >5k 2.053562325797024E-4 0.8968481385368434 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8982 0.4794455470066948 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7596 0.40546296760886813 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4767 0.2544552352016159 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.10088533554249088 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.3378484414016335E-5 0.0 10-11 0.0 0.0 0.0 5.3378484414016335E-5 0.0 12-13 0.0 0.0 0.0 5.3378484414016335E-5 0.0 14-15 0.0 0.0 0.0 5.3378484414016335E-5 0.0 16-17 0.0 0.0 0.0 5.3378484414016335E-5 0.0 18-19 0.0 0.0 0.0 1.60135453242049E-4 0.0 20-21 0.0 0.0 0.0 2.1351393765606534E-4 0.0 22-23 0.0 0.0 0.0 4.8040635972614704E-4 0.0 24-25 0.0 2.6689242207008168E-5 0.0 7.739880240032369E-4 0.0 26-27 0.0 5.3378484414016335E-5 0.0 0.0020283824077326207 0.0 28-29 0.0 5.3378484414016335E-5 0.0 0.004270278753121306 0.0 30-31 0.0 5.3378484414016335E-5 0.0 0.011289549453564456 0.0 32-33 0.0 5.3378484414016335E-5 0.0 0.028050393559565585 0.0 34-35 0.0 5.3378484414016335E-5 0.0 0.055353488337334944 0.0 36-37 0.0 5.3378484414016335E-5 0.0 0.09610796118743642 0.0 38-39 0.0 5.3378484414016335E-5 0.0 0.1515682064935994 0.0 40-41 0.0 5.3378484414016335E-5 0.0 0.2116723799437818 0.0 42-43 0.0 5.3378484414016335E-5 0.0 0.2742052744348019 0.0 44-45 0.0 5.3378484414016335E-5 0.0 0.3490685988254598 0.0 46-47 0.0 5.3378484414016335E-5 0.0 0.44135999837729406 0.0 48-49 0.0 5.3378484414016335E-5 0.0 0.538935867886116 0.0 50-51 0.0 5.3378484414016335E-5 0.0 0.6365117373949378 0.0 52-53 0.0 5.3378484414016335E-5 0.0 0.7341142961459667 0.0 54-55 0.0 5.3378484414016335E-5 0.0 0.836520918494257 0.0 56-57 0.0 5.3378484414016335E-5 0.0 0.9584640661380773 0.0 58-59 0.0 5.3378484414016335E-5 0.0 1.095166364722373 0.0 60-61 5.3378484414016335E-5 5.3378484414016335E-5 0.0 1.23194873103329 0.0 62-63 5.3378484414016335E-5 5.3378484414016335E-5 0.0 1.365688523732608 0.0 64-65 5.3378484414016335E-5 5.3378484414016335E-5 0.0 1.5099705671036943 0.0 66-67 5.3378484414016335E-5 5.3378484414016335E-5 0.0 1.6751235978806607 0.0 68-69 5.3378484414016335E-5 8.00677266210245E-5 0.0 1.8599199109219853 0.0 70-71 5.3378484414016335E-5 1.0675696882803267E-4 0.0 2.062678083968626 0.0 72-73 5.3378484414016335E-5 1.0675696882803267E-4 0.0 2.2712545118163954 0.0 74-75 5.3378484414016335E-5 1.0675696882803267E-4 0.0 2.483834325995215 0.0 76-77 5.3378484414016335E-5 1.0675696882803267E-4 0.0 2.715577016078667 0.0 78-79 5.3378484414016335E-5 1.0675696882803267E-4 0.0 2.9716336058127033 0.0 80-81 5.3378484414016335E-5 1.0675696882803267E-4 0.0 3.2541125453316777 0.0 82-83 5.3378484414016335E-5 1.0675696882803267E-4 0.0 3.5415289946589485 0.0 84-85 5.3378484414016335E-5 1.0675696882803267E-4 0.0 3.8142396715301583 0.0 86-87 5.3378484414016335E-5 1.0675696882803267E-4 0.0 4.108328431409181 0.0 88-89 5.3378484414016335E-5 1.60135453242049E-4 0.0 4.43126826211398 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4120 0.0 55.066338 1 GTATCAA 7675 0.0 42.1401 1 ATCAACG 9540 0.0 32.90374 3 CAACGCA 9690 0.0 32.491543 5 TATCAAC 9965 0.0 32.11995 2 TCAACGC 9890 0.0 31.882502 4 AACGCAG 10300 0.0 30.79781 6 GTGGTAT 1800 0.0 30.651413 1 ACGCAGA 11490 0.0 27.194838 7 CGCAGAG 11560 0.0 26.824766 8 TGGTATC 2035 0.0 26.136402 2 TATCGGC 280 0.0 25.440487 2 GTACATG 8855 0.0 24.224398 1 GCAGAGT 12455 0.0 24.020245 9 GGCGTCG 435 0.0 24.016739 8 GTACTAG 615 0.0 23.974613 1 TACATGG 8830 0.0 23.717564 2 ACATGGG 8745 0.0 23.459358 3 GAGTACT 7070 0.0 22.937807 12-13 GTACTTT 7560 0.0 21.19984 14-15 >>END_MODULE