FastQCFastQC Report
Sat 18 Jun 2016
SRR1931151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548517
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76480.3000960951015826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59100.2318995713977972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.13521589222281036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35500.060.748421
GTATCAA73300.046.1584851
TCAACGC92300.035.3161624
CAACGCA91200.034.993215
ATCAACG93350.034.7600633
AACGCAG95650.033.6966866
TATCAAC97900.033.679472
CGCAGAG107650.029.59248
ACGCAGA107250.029.5691857
GCAGAGT116250.025.8898879
GTACATG88700.025.879911
TACATGG89450.024.859772
ACATGGG85000.024.8135723
GTGGTAT18100.023.8294931
CAGAGTA115300.022.1641710-11
GAGTACT73100.021.99174512-13
TGGTATC18450.021.819382
ATCGACG1100.002802702121.6901843
AGAGTAC108800.021.0848610-11
GTACTAA5350.020.6027371
Produced by FastQC (version 0.11.5)