##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931151_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2548517 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.830306801955803 31.0 28.0 31.0 23.0 34.0 2 28.51667656130997 31.0 28.0 33.0 16.0 34.0 3 28.587066909893085 31.0 28.0 34.0 16.0 34.0 4 31.77153968366701 35.0 32.0 37.0 19.0 37.0 5 31.88837979107065 35.0 32.0 37.0 19.0 37.0 6 32.17688797053345 35.0 32.0 37.0 23.0 37.0 7 31.831779815477002 35.0 32.0 37.0 18.0 37.0 8 32.01202974121813 35.0 32.0 37.0 21.0 37.0 9 33.033762380239175 37.0 32.0 39.0 18.0 39.0 10-11 32.99587485584753 37.0 32.0 39.0 19.0 39.0 12-13 33.09013182960914 37.0 32.0 39.0 20.0 39.0 14-15 33.82133589848527 38.0 32.0 40.0 18.5 41.0 16-17 33.7915695677133 38.0 32.0 40.0 19.0 41.0 18-19 33.842357928159785 38.0 32.0 40.0 18.5 41.0 20-21 34.110061655464726 38.0 32.0 40.0 23.0 41.0 22-23 34.00059662148614 38.0 32.0 40.0 22.0 41.0 24-25 33.88810924157069 38.0 32.0 40.0 21.0 41.0 26-27 33.5155127472173 38.0 32.0 40.0 18.0 41.0 28-29 33.4902366748976 38.0 32.0 40.0 18.0 41.0 30-31 33.222380113611166 37.0 31.0 40.0 17.5 41.0 32-33 33.0084578600025 37.0 31.0 40.0 17.5 41.0 34-35 32.981040738594245 37.0 31.0 40.0 17.5 41.0 36-37 32.60590767101024 37.0 30.0 40.0 15.5 41.0 38-39 32.427181965040845 37.0 30.0 40.0 15.0 41.0 40-41 32.310114862879075 36.5 30.0 40.0 13.0 41.0 42-43 32.502398846074016 37.0 30.0 40.0 13.5 41.0 44-45 32.53346004754922 37.0 30.0 40.0 12.0 41.0 46-47 32.27485357170464 37.0 30.0 40.0 9.0 41.0 48-49 32.04097186716824 37.0 30.0 40.0 8.0 41.0 50-51 31.537971494794817 36.5 30.0 39.5 7.5 40.5 52-53 31.496499140480523 36.0 30.0 39.5 6.5 40.5 54-55 31.493754603167254 36.0 30.0 40.0 2.0 41.0 56-57 31.26922480799618 36.0 29.0 40.0 2.0 41.0 58-59 31.12771996419879 36.0 29.0 40.0 2.0 41.0 60-61 30.969804007585587 35.5 29.0 39.5 2.0 41.0 62-63 30.802656603820967 35.0 29.0 39.0 2.0 41.0 64-65 30.464770295823023 35.0 28.5 39.0 2.0 40.5 66-67 30.247017971628203 35.0 28.5 39.0 2.0 40.0 68-69 30.05587327845959 35.0 28.5 38.5 2.0 40.0 70-71 29.752194119168127 34.0 28.0 38.0 2.0 40.0 72-73 29.415251301050766 34.0 28.0 37.5 2.0 40.0 74-75 29.004029009812374 34.0 28.0 37.0 2.0 39.5 76-77 28.688550439333934 34.0 28.0 36.0 2.0 39.0 78-79 28.275089787511718 34.0 27.0 36.0 2.0 39.0 80-81 27.87943753170962 33.0 26.0 35.5 2.0 37.5 82-83 27.538647181870868 33.0 26.0 35.0 2.0 37.0 84-85 27.18661813909815 33.0 26.0 35.0 2.0 37.0 86-87 26.870709710784745 33.0 26.0 35.0 2.0 36.0 88-89 26.52084820309223 33.0 25.0 35.0 2.0 36.0 90-91 26.139850548377744 32.0 24.5 35.0 2.0 35.5 92-93 25.78815385575219 32.0 23.5 35.0 2.0 35.0 94-95 25.49257391651694 32.0 22.0 35.0 2.0 35.0 96-97 25.12217889855159 32.0 19.5 35.0 2.0 35.0 98-99 24.7662750532957 32.0 16.5 35.0 2.0 35.0 100-101 24.188436843858604 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.8565250221720255 1101 2 5.499043245636809 1101 3 5.171253745656898 1101 4 6.405864263634296 1101 5 5.834469307490021 1101 6 5.5122503067393325 1101 7 5.96842701702457 1101 8 5.48961149381563 1101 9 6.1317622744994935 1101 10-11 6.037898593610425 1101 12-13 5.899796377913127 1101 14-15 6.15911552399184 1101 16-17 6.124194707294276 1101 18-19 6.2196660132878065 1101 20-21 5.548530826114604 1101 22-23 5.642249442274736 1101 24-25 5.537520865295715 1101 26-27 5.593893834588748 1101 28-29 5.578025979288061 1101 30-31 5.562983526506265 1101 32-33 5.655505978042868 1101 34-35 5.32059473041803 1101 36-37 5.476278560579146 1101 38-39 5.390382341621972 1101 40-41 5.554460936690724 1101 42-43 5.3116125454107 1101 44-45 5.223168585981771 1101 46-47 4.9816173673763195 1101 48-49 4.923459379218713 1101 50-51 4.861081506599856 1101 52-53 4.791306913881311 1101 54-55 4.955988730117941 1101 56-57 4.861485823088984 1101 58-59 4.819499661305741 1101 60-61 4.733204789651808 1101 62-63 4.638871108262624 1101 64-65 4.6298321078149165 1101 66-67 4.639478331101888 1101 68-69 4.580921094592977 1101 70-71 4.385014579064052 1101 72-73 4.238671477675176 1101 74-75 4.364811831608197 1101 76-77 4.339454326624285 1101 78-79 4.204525832047114 1101 80-81 4.202714143057641 1101 82-83 4.046354835820452 1101 84-85 3.9250005717420464 1101 86-87 3.744161515723789 1101 88-89 3.900413834157206 1101 90-91 3.9126350626230177 1101 92-93 3.8659373681258913 1101 94-95 3.8676608272970157 1101 96-97 3.8042229455707677 1101 98-99 3.6366527517173566 1101 100-101 3.700547230938568 1102 1 5.3211752131403465 1102 2 5.8101428237590795 1102 3 5.594657425229528 1102 4 6.941103385928908 1102 5 6.500252377461809 1102 6 5.938618513634168 1102 7 6.441880672882181 1102 8 6.100228383902753 1102 9 6.879749842836198 1102 10-11 6.712316826716599 1102 12-13 6.7619800070601315 1102 14-15 6.9793925154294705 1102 16-17 6.873374666853916 1102 18-19 6.971810100758788 1102 20-21 6.192503516396446 1102 22-23 6.343018083528886 1102 24-25 6.147264293738754 1102 26-27 6.400132332803878 1102 28-29 6.317661691612376 1102 30-31 6.554289200636923 1102 32-33 6.364689831858492 1102 34-35 6.287936850790487 1102 36-37 6.408627520862723 1102 38-39 6.240411523578587 1102 40-41 6.40113641535968 1102 42-43 6.3221044698221895 1102 44-45 6.226854024959277 1102 46-47 6.225050503500537 1102 48-49 6.044317288802077 1102 50-51 5.990554683663685 1102 52-53 5.73843275326821 1102 54-55 6.101717580064278 1102 56-57 5.898702678227657 1102 58-59 5.999118997784436 1102 60-61 5.811157983690599 1102 62-63 5.81898399175947 1102 64-65 5.8448111637074724 1102 66-67 5.785656693018062 1102 68-69 5.722331164739511 1102 70-71 5.715468983817043 1102 72-73 5.594800662128112 1102 74-75 5.631381541286423 1102 76-77 5.477920039697501 1102 78-79 5.359609483660986 1102 80-81 5.3961416624665155 1102 82-83 5.269418417038153 1102 84-85 5.157746696208459 1102 86-87 5.22418825128883 1102 88-89 5.210800164320219 1102 90-91 5.159515339215041 1102 92-93 5.1913959417298265 1102 94-95 5.029556680912933 1102 96-97 4.9503984866476785 1102 98-99 4.7941402077197495 1102 100-101 4.76064533623326 1103 1 4.978934782558923 1103 2 5.550328314394289 1103 3 5.2120165995197745 1103 4 6.745862211901315 1103 5 6.28854702908939 1103 6 5.6569491660735025 1103 7 6.116736047092228 1103 8 5.69298459168818 1103 9 6.316353906359971 1103 10-11 6.234461452358385 1103 12-13 6.442755416529671 1103 14-15 6.555454743844802 1103 16-17 6.366863425693918 1103 18-19 6.429867522777073 1103 20-21 5.62089344549371 1103 22-23 5.887661352096192 1103 24-25 5.619304114361437 1103 26-27 5.827336886785808 1103 28-29 5.672196444103282 1103 30-31 5.885955266326981 1103 32-33 5.747926876408805 1103 34-35 5.66041891644182 1103 36-37 5.826270817729679 1103 38-39 5.70419199453714 1103 40-41 5.742952351165208 1103 42-43 5.667580987803408 1103 44-45 5.41411079550279 1103 46-47 5.587020557332597 1103 48-49 5.206282295122094 1103 50-51 5.113601816313835 1103 52-53 4.8831607034809785 1103 54-55 5.190075537963384 1103 56-57 5.291656800837451 1103 58-59 5.372371574546747 1103 60-61 5.171549554068896 1103 62-63 5.132895631356835 1103 64-65 4.976749720965547 1103 66-67 5.1906676278948325 1103 68-69 5.102812450446521 1103 70-71 5.026176910223526 1103 72-73 4.899996823905116 1103 74-75 4.922406516642759 1103 76-77 4.9704037904869125 1103 78-79 4.844935299329304 1103 80-81 4.862871125056127 1103 82-83 4.7122471198389135 1103 84-85 4.776077072071789 1103 86-87 4.7230362134595545 1103 88-89 4.66222312069149 1103 90-91 4.654586236263132 1103 92-93 4.596731804021452 1103 94-95 4.559109839289885 1103 96-97 4.487312122679292 1103 98-99 4.41353191929997 1103 100-101 4.337611054489532 1104 1 4.306866378845985 1104 2 5.165969090518889 1104 3 4.863229432503484 1104 4 6.275401827456324 1104 5 5.981765198567093 1104 6 5.6301418700751 1104 7 6.129370367377117 1104 8 5.41944713689854 1104 9 6.2490824787299815 1104 10-11 6.175946247391128 1104 12-13 6.117386458146534 1104 14-15 6.068070544658024 1104 16-17 5.970146567250506 1104 18-19 6.3023501854249275 1104 20-21 5.636973325506776 1104 22-23 5.741054937047412 1104 24-25 5.542063534879954 1104 26-27 5.872365996028087 1104 28-29 5.843165536788664 1104 30-31 6.016921771096584 1104 32-33 5.881271112980869 1104 34-35 5.617690767374022 1104 36-37 5.707574208524683 1104 38-39 5.514521169727978 1104 40-41 5.855241632046905 1104 42-43 5.784994991313926 1104 44-45 5.829731921746426 1104 46-47 5.622248536838999 1104 48-49 5.365382306522392 1104 50-51 5.290454713504797 1104 52-53 5.0923518932151275 1104 54-55 5.3670424621869905 1104 56-57 5.311422608890105 1104 58-59 5.275115270350234 1104 60-61 5.119100486223651 1104 62-63 5.038517108748888 1104 64-65 5.143392039738085 1104 66-67 5.190903811286667 1104 68-69 5.094201425251864 1104 70-71 5.034701182516521 1104 72-73 5.062778698681743 1104 74-75 5.115155133334429 1104 76-77 5.005534073767485 1104 78-79 4.940794249260829 1104 80-81 4.903465662629731 1104 82-83 4.6697508970896 1104 84-85 4.719466654893033 1104 86-87 4.663402215942607 1104 88-89 4.719551203474008 1104 90-91 4.76991417762423 1104 92-93 4.656507511573498 1104 94-95 4.525319956886122 1104 96-97 4.45646510457879 1104 98-99 4.378782583956596 1104 100-101 4.388935268867087 1105 1 4.401711283730808 1105 2 5.085329169605693 1105 3 4.789350912837289 1105 4 6.214290507579694 1105 5 3.541709583433068 1105 6 4.88333323643257 1105 7 5.573558383529782 1105 8 1.5417932052496575 1105 9 0.7371986461419375 1105 10-11 2.0998155171389747 1105 12-13 5.4708514197449105 1105 14-15 3.4958734867101704 1105 16-17 5.589980704652259 1105 18-19 6.184245493692913 1105 20-21 3.191773744065582 1105 22-23 5.3368827902858875 1105 24-25 5.443037377845247 1105 26-27 5.632196491274492 1105 28-29 3.100816086436158 1105 30-31 5.525114452676817 1105 32-33 5.715313992133574 1105 34-35 5.530620563384424 1105 36-37 5.791450878207215 1105 38-39 5.709688520984955 1105 40-41 5.916799260483465 1105 42-43 2.976499085848097 1105 44-45 5.187742929149248 1105 46-47 5.530232661666641 1105 48-49 5.374836012126394 1105 50-51 5.420579818650349 1105 52-53 5.38819492210089 1105 54-55 5.609513462247694 1105 56-57 5.487845723279797 1105 58-59 5.41549217561662 1105 60-61 5.219934424174042 1105 62-63 5.123619970731436 1105 64-65 5.20276943351578 1105 66-67 5.230805646528275 1105 68-69 5.37590450865433 1105 70-71 5.00719438238124 1105 72-73 5.029843208190368 1105 74-75 5.174354177124126 1105 76-77 5.018888643371678 1105 78-79 5.017041904262001 1105 80-81 5.061336042367785 1105 82-83 4.946212167392446 1105 84-85 4.913337427752616 1105 86-87 4.926054514402786 1105 88-89 5.007453611054316 1105 90-91 4.856879741912607 1105 92-93 4.832195257455119 1105 94-95 4.903944696370846 1105 96-97 4.960794499952097 1105 98-99 4.9272780269265155 1105 100-101 5.096113060969394 1106 1 4.131503859437899 1106 2 4.417241521265879 1106 3 4.558361721548138 1106 4 6.290866128549563 1106 5 6.241459874802647 1106 6 5.309496826915556 1106 7 6.048561074701805 1106 8 5.469773714416192 1106 9 6.050545863245134 1106 10-11 5.928644829995196 1106 12-13 6.167924002984073 1106 14-15 5.871070874136432 1106 16-17 5.822832508315923 1106 18-19 6.081555253537545 1106 20-21 5.374383470780675 1106 22-23 5.553670048237063 1106 24-25 5.487860928540666 1106 26-27 5.639310953970352 1106 28-29 5.7312540935614145 1106 30-31 5.905450998600102 1106 32-33 5.674832338214763 1106 34-35 5.290230071913747 1106 36-37 5.3197118155319885 1106 38-39 5.254307285539376 1106 40-41 5.544072899176193 1106 42-43 2.441498650440561 1106 44-45 4.534007662713574 1106 46-47 4.719563474027847 1106 48-49 4.681776279675489 1106 50-51 4.920451381461344 1106 52-53 4.6624967838777955 1106 54-55 4.91562472027984 1106 56-57 4.998518445592289 1106 58-59 4.927306115534236 1106 60-61 4.813262277692026 1106 62-63 4.777533288321546 1106 64-65 4.842681265900268 1106 66-67 4.943505439942669 1106 68-69 4.862688331545328 1106 70-71 4.881753761204113 1106 72-73 4.671556663761287 1106 74-75 4.7559460784797345 1106 76-77 4.61527199737214 1106 78-79 4.747009574850978 1106 80-81 4.667107586123674 1106 82-83 4.664042989554336 1106 84-85 4.732452043403445 1106 86-87 4.800580635159449 1106 88-89 4.928653137922176 1106 90-91 4.8801097781558695 1106 92-93 4.94143251664342 1106 94-95 4.827771803269339 1106 96-97 4.918428897082968 1106 98-99 4.743012112781166 1106 100-101 4.831771588353632 1107 1 3.036333310158632 1107 2 -0.07727497212050594 1107 3 1.0836481459173513 1107 4 2.6344497247756387 1107 5 2.7120915576179208 1107 6 4.112114313064623 1107 7 5.149447434679569 1107 8 4.535096727060598 1107 9 4.991346758942072 1107 10-11 2.5740494703138417 1107 12-13 4.979562249267943 1107 14-15 3.4497003340006778 1107 16-17 2.0483889308187564 1107 18-19 2.532184500800014 1107 20-21 2.3991786099386196 1107 22-23 4.174496733098419 1107 24-25 4.161694996731459 1107 26-27 3.1642213542389968 1107 28-29 3.951111556703058 1107 30-31 3.511220631757176 1107 32-33 4.197315382256626 1107 34-35 4.464563010963126 1107 36-37 4.733932297614629 1107 38-39 4.730256812576812 1107 40-41 3.555686321556543 1107 42-43 4.103266142917793 1107 44-45 4.351128448313755 1107 46-47 4.683735036321952 1107 48-49 2.4306931357881787 1107 50-51 3.801590041086623 1107 52-53 3.9118522838640963 1107 54-55 4.40675511307688 1107 56-57 3.3958298070897257 1107 58-59 2.4816906756771644 1107 60-61 3.3893581313568433 1107 62-63 4.339054262534521 1107 64-65 4.559965310921751 1107 66-67 4.468779340357273 1107 68-69 4.31178914724169 1107 70-71 4.07740781658789 1107 72-73 4.180659338704896 1107 74-75 4.396899827515366 1107 76-77 4.28971347736146 1107 78-79 4.172999112359658 1107 80-81 4.106621263656685 1107 82-83 4.0045733372855 1107 84-85 3.9246100317671626 1107 86-87 3.9350618924504523 1107 88-89 4.13403896787192 1107 90-91 4.191059461488187 1107 92-93 4.1924689247976765 1107 94-95 4.2957736592583196 1107 96-97 4.330942353970379 1107 98-99 4.315147240345787 1107 100-101 4.494269848491243 1108 1 -2.7218076294980484 1108 2 -2.0283637073284275 1108 3 -2.858014148003633 1108 4 -5.202130283059638 1108 5 -9.555747461426137 1108 6 -7.962738104985274 1108 7 -6.991668415831597 1108 8 -7.018073577759008 1108 9 -8.250008686645703 1108 10-11 -3.8388839558101573 1108 12-13 -2.5105771735524556 1108 14-15 -7.354696336948859 1108 16-17 -6.355389478118138 1108 18-19 -5.251315581804221 1108 20-21 -5.41528921478519 1108 22-23 -4.371395411167661 1108 24-25 -9.199796108119425 1108 26-27 -10.443560834804572 1108 28-29 -7.47243687006436 1108 30-31 -7.489005566881609 1108 32-33 -3.615217948337502 1108 34-35 -1.8537379212520406 1108 36-37 -5.731000921356774 1108 38-39 -6.91834499307469 1108 40-41 -4.420795368694417 1108 42-43 -5.4989545984202515 1108 44-45 -2.5259759266289983 1108 46-47 -3.1440854274430414 1108 48-49 -1.9545835655665584 1108 50-51 -1.3540459644294565 1108 52-53 -3.036858048627213 1108 54-55 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-2.3015712606800705 2313 60-61 -1.1746108131850725 2313 62-63 -3.1773251092314645 2313 64-65 -3.3105059536508072 2313 66-67 -4.07961623154587 2313 68-69 -4.652442971600877 2313 70-71 -4.785065799186537 2313 72-73 -5.585647671260993 2313 74-75 -6.397653889431989 2313 76-77 -4.94587427857666 2313 78-79 -6.141722394903237 2313 80-81 -7.447614462143303 2313 82-83 -7.249772823754018 2313 84-85 -6.791148364428167 2313 86-87 -6.14300869704217 2313 88-89 -7.806496331327427 2313 90-91 -7.914270374729231 2313 92-93 -7.047797856460395 2313 94-95 -7.197960922111967 2313 96-97 -7.077663579251926 2313 98-99 -7.10831178728974 2313 100-101 -6.806332201685546 2314 1 -13.585474977827975 2314 2 -14.691617573076643 2314 3 -14.177243330366492 2314 4 -25.111469069699037 2314 5 -19.185115487831617 2314 6 -18.36949823127236 2314 7 -18.842128538530982 2314 8 -14.437359266418285 2314 9 -16.556272813219806 2314 10-11 -14.781516611067932 2314 12-13 -16.08490537647284 2314 14-15 -17.847133014019857 2314 16-17 -18.371200029547836 2314 18-19 -16.949719951624473 2314 20-21 -17.597080284996505 2314 22-23 -18.02154295538608 2314 24-25 -19.206321239967448 2314 26-27 -16.827731896405407 2314 28-29 -18.328126067495567 2314 30-31 -19.398326415014207 2314 32-33 -16.368695776343095 2314 34-35 -17.65527076665799 2314 36-37 -16.81890857392378 2314 38-39 -16.144787249021306 2314 40-41 -18.612427952198168 2314 42-43 -18.805644764530825 2314 44-45 -15.089316794135186 2314 46-47 -14.327683802214324 2314 48-49 -14.182429509670172 2314 50-51 -13.766380481704235 2314 52-53 -13.68652349547542 2314 54-55 -14.23918378450194 2314 56-57 -12.974596048256048 2314 58-59 -11.900535426413557 2314 60-61 -11.990330298067493 2314 62-63 -14.577661055480064 2314 64-65 -12.685086020840055 2314 66-67 -14.143585996383493 2314 68-69 -16.435897618857314 2314 70-71 -16.51147957298273 2314 72-73 -15.234933785482717 2314 74-75 -13.665501033888876 2314 76-77 -14.058136316650568 2314 78-79 -14.024281185496744 2314 80-81 -13.786850184427738 2314 82-83 -14.346987269442707 2314 84-85 -14.498315217731637 2314 86-87 -14.496312168486739 2314 88-89 -13.600659265257995 2314 90-91 -13.100300609891605 2314 92-93 -13.140393041230832 2314 94-95 -13.083529231182517 2314 96-97 -12.722610387762565 2314 98-99 -12.660803388633523 2314 100-101 -12.432172067307047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77771.0 3 13288.0 4 8975.0 5 7164.0 6 7830.0 7 9210.0 8 10892.0 9 12769.0 10 13571.0 11 14820.0 12 16265.0 13 17743.0 14 19984.0 15 22758.0 16 25294.0 17 26782.0 18 24729.0 19 21599.0 20 21171.0 21 22172.0 22 25724.0 23 31109.0 24 38709.0 25 47178.0 26 55812.0 27 64666.0 28 71220.0 29 79273.0 30 90057.0 31 100681.0 32 115876.0 33 138776.0 34 168438.0 35 203860.0 36 252515.0 37 310374.0 38 287939.0 39 71462.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.10419318238458 20.814774783684726 14.386010768373994 22.695021265556704 2 14.716219657472093 20.942089715096188 41.22811305328869 23.11357757414303 3 17.3500117911711 27.123146520113462 31.26402531354509 24.262816375170342 4 11.137002707769215 18.1567287118214 41.15623796810008 29.550030612309303 5 11.716437432021259 38.60000668723976 36.31965792801776 13.363897952721215 6 28.21753032393579 39.538885295144595 19.033569499283413 13.210014881636203 7 25.553510318365653 33.23080959967821 23.618718555460234 17.596961526495907 8 24.626924886889203 35.573983770496056 21.6474305642302 18.151660778384546 9 25.589564832098343 16.40081452570847 22.294066720716984 35.7155539214762 10-11 23.01094981206807 27.279095757961986 29.97567777979246 19.73427665017748 12-13 24.0467475395799 25.321053199563377 29.403619761674353 21.228579499182366 14-15 21.80272635972777 26.646423540952064 27.443695492005578 24.107154607314595 16-17 20.56958569514988 29.596733327833885 28.21407387330057 21.619607103715662 18-19 20.324931242845885 28.559847473229873 30.532422455434887 20.582798828489356 20-21 21.42368519202657 27.98870523204966 30.474819019157383 20.112790556766384 22-23 21.209943824836234 27.61846054993769 30.205334107314474 20.9662615179116 24-25 20.833053493908142 28.038673219425924 30.312088709686925 20.816184576979012 26-27 20.635671087716705 28.178139195844366 30.321379958937474 20.864809757501458 28-29 20.578542881318516 27.8386025752125 30.66740413269046 20.915450410778526 30-31 21.03623808293794 27.730103527558402 30.46845213483234 20.765206254671313 32-33 20.749955812172274 28.177890374909172 30.242738466976967 20.829415345941594 34-35 21.054499080420975 28.100492177635044 30.28410188240116 20.560906859542825 36-37 21.06368620255067 27.62055663664395 30.18175709990206 21.13400006090331 38-39 20.74940862165872 28.351171765031303 30.122389433712875 20.777030179597098 40-41 20.85095551609182 27.917563733479145 30.44944274818456 20.782038002244484 42-43 20.768512524472293 28.124597365948397 29.99008931012485 21.11680079945446 44-45 20.87331644790095 28.159017885231165 30.027248673404344 20.94041699346354 46-47 21.024736890579117 28.064657963153195 29.628517559840173 21.282087586427515 48-49 20.84859587257472 28.313322812229725 29.773518712141506 21.064562603054053 50-51 20.76260769370336 28.337120465357756 29.434302226488036 21.46596961445085 52-53 20.857081700142178 28.682358393489753 29.257699890815115 21.202860015552954 54-55 20.993663384184615 28.442940839950413 29.241084035778197 21.322311740086775 56-57 20.98329678808612 28.67248241300985 28.949563622191693 21.394657176712347 58-59 20.82074351656836 28.50700013169724 29.332145367770952 21.34011098396345 60-61 21.22414890857498 28.780822833067386 28.877722667211657 21.11730559114598 62-63 20.92558142637463 28.645011981477857 29.24065250496661 21.188754087180897 64-65 20.85263704342565 28.324099858859093 29.291348654923627 21.53191444279163 66-67 20.98959904917252 28.521077159775665 28.927038744493366 21.56228504655845 68-69 20.93523409888967 28.435811885892853 29.080225872536854 21.548728142680627 70-71 21.05646146366691 28.153726265118106 29.17261293528746 21.617199335927523 72-73 21.197425797041966 28.279211007813565 28.990134262396523 21.533228932747946 74-75 20.853304098030346 28.691156464720464 28.84791429682439 21.607625140424805 76-77 20.853676863838853 28.477502798686448 29.010773716635992 21.658046620838707 78-79 20.800410591728443 28.47193093081192 28.838555913105544 21.889102564354094 80-81 20.92065699385172 28.53161269867927 28.742715861812968 21.80501444565604 82-83 21.236782018719122 28.342934341815262 28.86039214178285 21.55989149768277 84-85 21.018517828211465 28.310680289752828 28.66392494144634 22.006876940589372 86-87 20.71341882357465 28.58644851103603 28.909714944024312 21.790417721365014 88-89 21.104783684001323 28.239619355099453 28.856644864444696 21.798952096454528 90-91 20.99366023455994 28.183469052786386 29.070337768984867 21.752532943668808 92-93 21.242903225679875 28.23084954897299 28.843029102807634 21.6832181225395 94-95 20.861230276274398 28.014978907341014 28.976695074037174 22.14709574234741 96-97 21.04727965322578 27.954688942628202 28.898885116324514 22.099146287821505 98-99 20.78240011740161 28.080958455446837 28.70661643614698 22.430024991004572 100-101 20.838962424029347 28.034578541167278 28.86572857862043 22.260730456182948 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 385.0 1 261.0 2 204.0 3 260.0 4 231.0 5 243.5 6 365.5 7 725.5 8 1275.5 9 1653.0 10 1612.5 11 1507.5 12 1611.0 13 1796.0 14 2041.0 15 2330.5 16 2592.5 17 2842.0 18 3209.5 19 3617.0 20 4059.0 21 4797.5 22 5802.5 23 7266.0 24 9250.5 25 12080.5 26 16789.5 27 22332.5 28 26789.5 29 32849.5 30 41188.0 31 49039.5 32 58808.0 33 69958.0 34 81196.0 35 90697.5 36 101078.0 37 109506.5 38 108520.0 39 105571.5 40 102712.0 41 98656.0 42 93726.0 43 92258.0 44 93229.5 45 91778.5 46 86567.5 47 81042.5 48 79134.5 49 76390.5 50 72447.0 51 68053.0 52 63664.5 53 59786.0 54 56194.0 55 52421.5 56 47933.0 57 42946.5 58 38387.5 59 34058.5 60 28850.0 61 23640.0 62 18732.0 63 14062.0 64 10523.0 65 7834.5 66 5925.0 67 4625.0 68 3782.0 69 3103.0 70 2375.0 71 1793.5 72 1368.5 73 1006.0 74 774.0 75 617.5 76 458.0 77 335.0 78 262.5 79 202.0 80 149.0 81 111.0 82 91.5 83 86.5 84 64.5 85 53.0 86 47.5 87 32.5 88 18.5 89 9.5 90 11.0 91 10.5 92 8.0 93 6.0 94 4.0 95 2.5 96 2.0 97 1.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019619253079339868 2 0.46030691574747196 3 0.0 4 0.533212060190299 5 0.24963537618152049 6 0.09519261594095704 7 0.10849446952874947 8 0.10920076263960571 9 0.10755274538094114 10-11 0.1300167901567853 12-13 0.19213134540597532 14-15 0.11620483598893004 16-17 0.110142486787414 18-19 0.19476032531860688 20-21 0.10737617210322709 22-23 0.09315221362070569 24-25 0.09472175386705288 26-27 0.19929237277993436 28-29 0.11233984313230008 30-31 0.12005020959248065 32-33 0.10072524530933087 34-35 0.14135671843664374 36-37 0.13731515230229974 38-39 0.13354825571106646 40-41 0.10672873675160888 42-43 0.10912228562728833 44-45 0.09173962739899322 46-47 0.0934072639107371 48-49 0.09899875103834897 50-51 0.08534375089512841 52-53 0.09326992913918172 54-55 0.306354636833892 56-57 0.32516950053697896 58-59 0.33747077221772503 60-61 0.16232969997845806 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2548517.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.76472220723587 #Duplication Level Percentage of deduplicated Percentage of total 1 86.00976243751461 53.12369084063441 2 8.723260463422616 10.775795185293227 3 2.2086914790522187 4.092576469354476 4 0.8550054409935183 2.112366941945594 5 0.4574162273863383 1.4126093108799511 6 0.29007274694128904 1.0749757580831127 7 0.19476468541094075 0.8420710683130508 8 0.14050046046784814 0.6942377528628292 9 0.11607741379489295 0.6452540295818339 >10 0.8235274579348568 10.2338415904642 >50 0.10272858009741012 4.431937415286477 >100 0.07498838768077744 8.506608938954122 >500 0.0027369373210845943 1.1542142869148615 >1k 3.3377284403457485E-4 0.3408135777536382 >5k 1.3350913761382994E-4 0.5590068336781789 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7648 0.3000960951015826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5910 0.2318995713977972 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3446 0.13521589222281036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.9238506158679734E-5 0.0 10-11 3.9238506158679734E-5 0.0 0.0 1.1771551847603921E-4 0.0 12-13 3.9238506158679734E-5 0.0 0.0 1.5695402463471894E-4 0.0 14-15 3.9238506158679734E-5 0.0 0.0 1.765732777140588E-4 0.0 16-17 3.9238506158679734E-5 0.0 0.0 1.9619253079339866E-4 0.0 18-19 3.9238506158679734E-5 0.0 0.0 1.9619253079339866E-4 1.9619253079339867E-5 20-21 3.9238506158679734E-5 0.0 0.0 2.550502900314183E-4 7.847701231735947E-5 22-23 3.9238506158679734E-5 0.0 0.0 3.531465554281176E-4 7.847701231735947E-5 24-25 3.9238506158679734E-5 0.0 0.0 7.455316170149149E-4 7.847701231735947E-5 26-27 3.9238506158679734E-5 0.0 0.0 0.0014125862217124704 7.847701231735947E-5 28-29 3.9238506158679734E-5 0.0 0.0 0.0032371767580910782 7.847701231735947E-5 30-31 7.847701231735947E-5 0.0 0.0 0.009103333428813698 7.847701231735947E-5 32-33 7.847701231735947E-5 0.0 0.0 0.02497530916999965 7.847701231735947E-5 34-35 7.847701231735947E-5 0.0 0.0 0.05142206232094979 7.847701231735947E-5 36-37 7.847701231735947E-5 0.0 0.0 0.093054117355309 1.1771551847603921E-4 38-39 7.847701231735947E-5 0.0 0.0 0.15036195560006074 1.1771551847603921E-4 40-41 7.847701231735947E-5 0.0 0.0 0.20971019616506384 1.1771551847603921E-4 42-43 7.847701231735947E-5 1.9619253079339867E-5 0.0 0.2746499238576788 1.1771551847603921E-4 44-45 7.847701231735947E-5 3.9238506158679734E-5 0.0 0.3525187393295788 1.1771551847603921E-4 46-47 9.809626539669935E-5 5.8857759238019604E-5 0.0 0.44543552191333236 1.1771551847603921E-4 48-49 1.1771551847603921E-4 7.847701231735947E-5 0.0 0.5439830301308565 1.1771551847603921E-4 50-51 1.1771551847603921E-4 7.847701231735947E-5 0.0 0.6436880742800617 1.1771551847603921E-4 52-53 1.1771551847603921E-4 7.847701231735947E-5 0.0 0.7437070264785364 1.1771551847603921E-4 54-55 1.1771551847603921E-4 7.847701231735947E-5 0.0 0.8512597718594774 1.1771551847603921E-4 56-57 1.1771551847603921E-4 7.847701231735947E-5 0.0 0.9814531352939768 1.1771551847603921E-4 58-59 1.5695402463471894E-4 7.847701231735947E-5 0.0 1.1265178925626158 1.1771551847603921E-4 60-61 1.5695402463471894E-4 7.847701231735947E-5 0.0 1.2778019530574056 1.1771551847603921E-4 62-63 1.5695402463471894E-4 7.847701231735947E-5 0.0 1.4300669762061622 1.1771551847603921E-4 64-65 1.5695402463471894E-4 7.847701231735947E-5 0.0 1.5900816043212582 1.1771551847603921E-4 66-67 1.9619253079339866E-4 7.847701231735947E-5 0.0 1.77191284186058 1.1771551847603921E-4 68-69 1.9619253079339866E-4 7.847701231735947E-5 0.0 1.973029805176893 1.1771551847603921E-4 70-71 1.9619253079339866E-4 7.847701231735947E-5 0.0 2.192686962653182 1.1771551847603921E-4 72-73 2.3543103695207842E-4 7.847701231735947E-5 0.0 2.412128308345599 1.1771551847603921E-4 74-75 2.3543103695207842E-4 7.847701231735947E-5 0.0 2.6371022834063886 1.1771551847603921E-4 76-77 2.3543103695207842E-4 7.847701231735947E-5 0.0 2.878065949726841 1.1771551847603921E-4 78-79 2.3543103695207842E-4 7.847701231735947E-5 0.0 3.143553682396468 1.1771551847603921E-4 80-81 2.3543103695207842E-4 7.847701231735947E-5 0.0 3.433722435439905 1.3733477155537907E-4 82-83 2.3543103695207842E-4 7.847701231735947E-5 0.0 3.731111073616539 1.5695402463471894E-4 84-85 2.3543103695207842E-4 7.847701231735947E-5 0.0 4.0330317592544995 1.5695402463471894E-4 86-87 2.3543103695207842E-4 7.847701231735947E-5 0.0 4.34240776106261 1.5695402463471894E-4 88-89 2.3543103695207842E-4 7.847701231735947E-5 0.0 4.682782182736078 1.5695402463471894E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3550 0.0 60.74842 1 GTATCAA 7330 0.0 46.158485 1 TCAACGC 9230 0.0 35.316162 4 CAACGCA 9120 0.0 34.99321 5 ATCAACG 9335 0.0 34.760063 3 AACGCAG 9565 0.0 33.696686 6 TATCAAC 9790 0.0 33.67947 2 CGCAGAG 10765 0.0 29.5924 8 ACGCAGA 10725 0.0 29.569185 7 GCAGAGT 11625 0.0 25.889887 9 GTACATG 8870 0.0 25.87991 1 TACATGG 8945 0.0 24.85977 2 ACATGGG 8500 0.0 24.813572 3 GTGGTAT 1810 0.0 23.829493 1 CAGAGTA 11530 0.0 22.16417 10-11 GAGTACT 7310 0.0 21.991745 12-13 TGGTATC 1845 0.0 21.81938 2 ATCGACG 110 0.0028027021 21.690184 3 AGAGTAC 10880 0.0 21.08486 10-11 GTACTAA 535 0.0 20.602737 1 >>END_MODULE