FastQCFastQC Report
Sat 18 Jun 2016
SRR1931151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548517
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97660.3832032511456663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80560.31610540561432393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50440.19791902506438055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.060.098741
GTATCAA79600.042.8433231
TCAACGC102650.032.5224084
CAACGCA103500.032.3011975
ATCAACG103800.032.073113
AACGCAG108250.031.1031686
TATCAAC108950.030.949352
ACGCAGA123900.026.9445117
CGCAGAG124250.026.6010728
ACGTGCG1457.9109304E-726.2004249
GTGGTAT19650.024.9316881
GCAGAGT133500.024.188789
GTACATG90350.023.2160031
TGGTATC20800.022.832692
TACATGG94800.021.5417292
ACATGGG89300.021.5389213
GAGTACT87050.021.49382812-13
CAGAGTA133700.021.09800310-11
AGAGTAC127450.021.07070510-11
GTACTTT92600.020.12866814-15
Produced by FastQC (version 0.11.5)