##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931151_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2548517 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.345232148735914 31.0 31.0 34.0 30.0 34.0 2 31.380473035887146 31.0 31.0 34.0 30.0 34.0 3 31.52625703497367 31.0 31.0 34.0 30.0 34.0 4 35.15346336712684 37.0 35.0 37.0 33.0 37.0 5 35.0673995896437 37.0 35.0 37.0 32.0 37.0 6 35.17788148950939 37.0 35.0 37.0 32.0 37.0 7 35.04622649172048 37.0 35.0 37.0 32.0 37.0 8 35.0764581911755 37.0 35.0 37.0 32.0 37.0 9 36.49017408948028 38.0 35.0 39.0 32.0 39.0 10-11 36.38541159427228 38.0 35.0 39.0 32.0 39.0 12-13 36.310191966543684 38.0 35.0 39.0 32.0 39.0 14-15 37.262832855342936 39.0 36.0 41.0 32.0 41.0 16-17 37.292586629792936 39.0 36.0 41.0 32.0 41.0 18-19 37.39915213435893 39.0 36.0 41.0 32.0 41.0 20-21 37.40307206112418 39.0 36.0 41.0 32.0 41.0 22-23 37.28905457566106 39.0 36.0 40.5 31.5 41.0 24-25 37.156325423766056 39.0 36.0 40.5 31.0 41.0 26-27 36.93933393420566 39.0 36.0 40.0 31.0 41.0 28-29 36.67603139394401 39.0 35.0 40.0 30.0 41.0 30-31 36.44803134528826 38.0 35.0 40.0 30.0 41.0 32-33 36.302095689375435 38.0 35.0 40.0 30.0 41.0 34-35 36.083279020701056 38.0 35.0 40.0 30.0 41.0 36-37 36.311521170939805 38.0 35.0 40.0 30.0 41.0 38-39 36.28478130614785 38.0 35.0 40.0 30.0 41.0 40-41 36.14298413547958 38.0 35.0 40.0 30.0 41.0 42-43 35.95066719193947 38.0 35.0 40.0 29.0 41.0 44-45 35.71059816355944 38.0 35.0 40.0 28.5 41.0 46-47 35.48454414861663 38.0 34.5 40.0 27.5 41.0 48-49 35.201035739608564 38.0 34.0 40.0 27.0 41.0 50-51 34.859219695218826 38.0 34.0 40.0 26.0 41.0 52-53 34.76409613904871 38.0 34.0 40.0 26.0 41.0 54-55 34.53977980135114 38.0 33.0 40.0 25.0 41.0 56-57 34.34025415565209 38.0 33.0 40.0 24.5 41.0 58-59 34.11278029536393 37.5 33.0 40.0 24.0 41.0 60-61 33.849023373200964 37.0 33.0 40.0 23.0 41.0 62-63 33.59143278228083 37.0 32.5 40.0 22.5 41.0 64-65 33.36794339610056 36.5 32.0 39.5 22.0 41.0 66-67 33.13596083526224 36.0 32.0 39.0 22.0 41.0 68-69 32.80979860051944 36.0 31.5 39.0 22.0 40.0 70-71 31.992106389716056 35.0 31.0 38.5 18.5 40.0 72-73 31.97110535264234 35.0 31.0 38.0 20.0 40.0 74-75 31.664188624207725 35.0 31.0 37.0 19.5 39.5 76-77 30.936746350917026 34.0 30.0 36.0 18.0 39.0 78-79 30.95300404117375 34.0 30.0 36.0 18.0 39.0 80-81 30.67945220691092 34.0 30.0 36.0 17.0 38.5 82-83 30.313397556304313 34.0 30.0 35.5 13.0 37.0 84-85 29.94804841403844 34.0 30.0 35.0 9.5 37.0 86-87 29.637207442602893 34.0 29.0 35.0 7.0 36.5 88-89 29.322390433338292 34.0 29.0 35.0 4.5 36.0 90-91 29.02214680145355 34.0 29.0 35.0 2.0 36.0 92-93 28.699592743544578 34.0 29.0 35.0 2.0 35.5 94-95 28.331469242700756 34.0 29.0 35.0 2.0 35.0 96-97 27.983507859668975 34.0 29.0 35.0 2.0 35.0 98-99 27.536855944064726 33.0 27.0 35.0 2.0 35.0 100-101 26.902747754870774 33.0 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8074652633184094 1101 2 0.7308246754273782 1101 3 0.46873333824039776 1101 4 0.7904640131219764 1101 5 0.7893633300717298 1101 6 0.9260206416309344 1101 7 0.7471731663102688 1101 8 0.7073261634143719 1101 9 1.0703152110810024 1101 10-11 0.7618525864045793 1101 12-13 0.9452558499848749 1101 14-15 1.2738363209011823 1101 16-17 1.0430865767254502 1101 18-19 0.8051618306527146 1101 20-21 0.8938333994002434 1101 22-23 0.7471927416060353 1101 24-25 0.7455728567023598 1101 26-27 1.0109117771766734 1101 28-29 1.0398869981607533 1101 30-31 0.9861647086375243 1101 32-33 1.2107689730266316 1101 34-35 0.8776105044317788 1101 36-37 1.153322229699647 1101 38-39 0.8919336099717441 1101 40-41 0.9059350424793706 1101 42-43 1.0862047767202867 1101 44-45 1.0230062205731585 1101 46-47 1.049763053270695 1101 48-49 0.9936013594687694 1101 50-51 0.9562632796761932 1101 52-53 0.7284206107665128 1101 54-55 0.7811882944861921 1101 56-57 0.6482486956653233 1101 58-59 0.8064128465913853 1101 60-61 1.0271939515043655 1101 62-63 0.8962275997173563 1101 64-65 0.7588330710706757 1101 66-67 0.5364081708417174 1101 68-69 0.48611195983665567 1101 70-71 0.8742658060671076 1101 72-73 0.5087968878051896 1101 74-75 0.15487820057343527 1101 76-77 0.15118537779014218 1101 78-79 0.25742096829307215 1101 80-81 0.2216480910726908 1101 82-83 0.16598926787905555 1101 84-85 0.13375430754924267 1101 86-87 0.08048107866906662 1101 88-89 -0.029191470663434416 1101 90-91 0.06504176203771195 1101 92-93 -0.22572141009212032 1101 94-95 -0.23383520058981944 1101 96-97 -0.29774790365991777 1101 98-99 -0.35896286476612715 1101 100-101 -0.5145357733128044 1102 1 0.8861748666534908 1102 2 0.8107101443607014 1102 3 0.6036373548159517 1102 4 0.8126684615605342 1102 5 0.9397818627360479 1102 6 1.0064828099326917 1102 7 0.9327721577978281 1102 8 0.9419669682272769 1102 9 1.2326581552383686 1102 10-11 1.0925680311236192 1102 12-13 1.2153800917282993 1102 14-15 1.547406804437962 1102 16-17 1.3384026355386993 1102 18-19 1.1502943851348562 1102 20-21 1.1585620995674972 1102 22-23 0.8264667124989629 1102 24-25 0.6250220441508745 1102 26-27 1.0190531935874674 1102 28-29 1.2360583953499003 1102 30-31 1.4261155561374537 1102 32-33 1.456670143799741 1102 34-35 1.2553865847133636 1102 36-37 1.58737055841479 1102 38-39 1.3051185871304298 1102 40-41 1.3256297677595796 1102 42-43 1.4526667453166127 1102 44-45 1.4343740779834704 1102 46-47 1.5289509760845448 1102 48-49 1.2285442187513382 1102 50-51 1.1463177742966266 1102 52-53 0.9615477732019215 1102 54-55 0.9764923460101969 1102 56-57 1.205252015768167 1102 58-59 1.2508462323473921 1102 60-61 1.5608714771540377 1102 62-63 1.3846360722204167 1102 64-65 1.3341990562425465 1102 66-67 0.9893878008873997 1102 68-69 0.9290034199330108 1102 70-71 1.3295963435243507 1102 72-73 0.9405453088840972 1102 74-75 0.7507173128827773 1102 76-77 0.8252796137920413 1102 78-79 0.8773173960621143 1102 80-81 0.6345445188417322 1102 82-83 0.6974018343435233 1102 84-85 0.7532483288678016 1102 86-87 0.6863438560723942 1102 88-89 0.669925431905277 1102 90-91 0.6529812762542413 1102 92-93 0.48561948708454494 1102 94-95 0.5512958061752009 1102 96-97 0.5945883254022135 1102 98-99 0.5472679447897804 1102 100-101 0.42860317174323725 1103 1 0.6779122968544762 1103 2 0.47811633904087003 1103 3 0.3015630199775323 1103 4 0.6071639471734187 1103 5 0.675599230513825 1103 6 0.7499078451677335 1103 7 0.5904131946437232 1103 8 0.6562410409210315 1103 9 0.8756376018384344 1103 10-11 0.7788861224686627 1103 12-13 0.9292472766720579 1103 14-15 1.2226905257325171 1103 16-17 1.0266100373150735 1103 18-19 0.751263171606773 1103 20-21 0.8128689139211431 1103 22-23 0.6353333341660559 1103 24-25 0.43123380782714804 1103 26-27 0.8518136359490498 1103 28-29 0.7127472116386855 1103 30-31 1.0322800697669479 1103 32-33 1.1952697180011143 1103 34-35 0.8392545287594686 1103 36-37 1.1239863072746914 1103 38-39 0.9591821627672203 1103 40-41 0.8511842791858513 1103 42-43 1.091453793598241 1103 44-45 1.0006939907790624 1103 46-47 1.0757323994029377 1103 48-49 0.9118843520678865 1103 50-51 0.8086632454806448 1103 52-53 0.2545474518304829 1103 54-55 0.6002038290359124 1103 56-57 0.6685340819529131 1103 58-59 0.821103328015873 1103 60-61 1.0046838027537248 1103 62-63 0.884597326885725 1103 64-65 0.7859920071005675 1103 66-67 0.6504525029293546 1103 68-69 0.36568771519191756 1103 70-71 0.8627959836397068 1103 72-73 0.4187304507372147 1103 74-75 0.28554870202674465 1103 76-77 0.3690588786816704 1103 78-79 0.376093423777796 1103 80-81 0.2815948925675258 1103 82-83 0.30628002775302576 1103 84-85 0.2620532987805291 1103 86-87 0.29180329844875175 1103 88-89 0.07198036197377888 1103 90-91 0.14716557435299293 1103 92-93 -0.10727303315946202 1103 94-95 -0.142522409011697 1103 96-97 -0.2199920354593381 1103 98-99 -0.46066287209374224 1103 100-101 -0.47912391234213914 1104 1 0.5413082003473448 1104 2 0.4223938463488466 1104 3 0.17737423296444632 1104 4 0.602989177235763 1104 5 0.5130229459239075 1104 6 0.6899977872667264 1104 7 0.5237279924712226 1104 8 0.48163494046275446 1104 9 0.7421537717423163 1104 10-11 0.5849646700418276 1104 12-13 0.7756956749301693 1104 14-15 1.0982151198314014 1104 16-17 0.8898193751694023 1104 18-19 0.6490239750728506 1104 20-21 0.6531621132344299 1104 22-23 0.3857052628800446 1104 24-25 0.42857431549156644 1104 26-27 0.28919016278329224 1104 28-29 0.6300061326124933 1104 30-31 0.8692152799966237 1104 32-33 1.0139868188812358 1104 34-35 0.7527804533741573 1104 36-37 0.9650761805870758 1104 38-39 0.644532442940374 1104 40-41 0.8080884584774282 1104 42-43 1.050999330573923 1104 44-45 1.0125503489466112 1104 46-47 1.026282139096125 1104 48-49 0.8434511041806658 1104 50-51 0.7998448503059095 1104 52-53 0.4813593416199069 1104 54-55 0.9224829699055945 1104 56-57 0.798788933934226 1104 58-59 0.8553428247095454 1104 60-61 1.187385052890214 1104 62-63 0.2165132792699893 1104 64-65 -0.47316255256171047 1104 66-67 0.09104639111888702 1104 68-69 0.462679671968921 1104 70-71 1.0431697691111097 1104 72-73 0.6902872841095942 1104 74-75 0.5918179039529647 1104 76-77 0.22003706755430485 1104 78-79 0.2052612308751307 1104 80-81 0.2764927300223654 1104 82-83 0.09969751003806593 1104 84-85 0.07532420786531802 1104 86-87 0.2955185209253237 1104 88-89 0.31395659644087104 1104 90-91 0.48260388214468364 1104 92-93 0.458483549791179 1104 94-95 0.5440741113479355 1104 96-97 0.46879209147745016 1104 98-99 0.3781633205001036 1104 100-101 0.0845697457730239 1105 1 -0.21296685175571284 1105 2 -1.7736925710132496 1105 3 -0.3852751748962504 1105 4 0.4243134186701951 1105 5 0.6540208964888734 1105 6 0.18150838564972105 1105 7 -0.11920434726671658 1105 8 -0.07234035647407211 1105 9 0.6804828318060814 1105 10-11 0.9015869178297891 1105 12-13 1.0504883466823642 1105 14-15 1.3088752171289784 1105 16-17 0.8203303753457334 1105 18-19 0.6655251067425425 1105 20-21 1.0710195141808114 1105 22-23 0.39596009813032396 1105 24-25 0.042047977794389624 1105 26-27 1.13778085100612 1105 28-29 0.6533186729002267 1105 30-31 0.9309962072431333 1105 32-33 1.0579119350110773 1105 34-35 0.6312467880072958 1105 36-37 0.7584585866642755 1105 38-39 0.7497112409713012 1105 40-41 0.99142704306648 1105 42-43 1.2749028539256315 1105 44-45 1.2227706415330815 1105 46-47 1.3504709646166475 1105 48-49 1.3592305976898231 1105 50-51 0.9334761080924565 1105 52-53 0.9814496728537065 1105 54-55 0.1468708132004224 1105 56-57 0.564316800758526 1105 58-59 -0.0017628469356978371 1105 60-61 0.8161732557756167 1105 62-63 1.2339914335644124 1105 64-65 1.5762412612952446 1105 66-67 1.310014071326087 1105 68-69 1.1647444271784977 1105 70-71 0.8248899070505189 1105 72-73 0.2106125343632428 1105 74-75 0.2485569030539878 1105 76-77 0.4969342402589554 1105 78-79 0.08280141743442115 1105 80-81 0.16737332224838397 1105 82-83 0.16606148276236965 1105 84-85 0.14357039444195152 1105 86-87 -0.04784726420485086 1105 88-89 -0.3592857022794611 1105 90-91 -0.33199581318612914 1105 92-93 -0.0233678213646904 1105 94-95 -0.39252579210603855 1105 96-97 -0.5290000686386378 1105 98-99 -1.2200372813227105 1105 100-101 -0.5612228421517855 1106 1 0.4522802042627596 1106 2 0.398579263389216 1106 3 -0.2368446503538877 1106 4 0.40855012355739717 1106 5 0.20894699449509346 1106 6 0.5907595264656607 1106 7 0.4868826179170682 1106 8 0.5461768350541476 1106 9 0.963384104834887 1106 10-11 0.32132527485561724 1106 12-13 0.4245803452415018 1106 14-15 0.2069307288581541 1106 16-17 -0.05849384436701399 1106 18-19 0.4847495222119491 1106 20-21 0.501998133582255 1106 22-23 0.5600767654985717 1106 24-25 0.439068761997099 1106 26-27 0.8250434293834914 1106 28-29 0.7411554108205607 1106 30-31 0.21225327859887244 1106 32-33 0.22990070631672666 1106 34-35 -1.0411453538346649 1106 36-37 -0.7435462802600838 1106 38-39 -0.8127855175722516 1106 40-41 0.5267548213923163 1106 42-43 0.6599121742175171 1106 44-45 0.4323303914966985 1106 46-47 0.009019492633925097 1106 48-49 0.07755595283616401 1106 50-51 0.6687358600162057 1106 52-53 0.024817837718593694 1106 54-55 0.6899773158110918 1106 56-57 0.3296821398124621 1106 58-59 0.7355913919574775 1106 60-61 1.0205496638209155 1106 62-63 0.815967457551352 1106 64-65 0.8358445038120976 1106 66-67 0.44365352882436326 1106 68-69 0.45831469504571487 1106 70-71 1.0017786037102852 1106 72-73 0.46391756674245954 1106 74-75 0.45315001873068184 1106 76-77 0.5358876940689612 1106 78-79 0.448152906994121 1106 80-81 0.49156344013617925 1106 82-83 0.5651842731494696 1106 84-85 0.5640786676670864 1106 86-87 0.6098569266649569 1106 88-89 0.49826235241286554 1106 90-91 0.6774450432074701 1106 92-93 0.2771274506879031 1106 94-95 0.42553783651928967 1106 96-97 0.655375613191886 1106 98-99 0.6844491193415543 1106 100-101 0.4803338988404988 1107 1 0.4985257721319556 1107 2 0.5436123597211306 1107 3 0.25179831196255265 1107 4 -1.2882099090211767 1107 5 0.2530079977616069 1107 6 0.4801030564023421 1107 7 0.46065114407874574 1107 8 0.6250213403828866 1107 9 0.8163895332055517 1107 10-11 0.8655719482947148 1107 12-13 1.0130653937270253 1107 14-15 1.1476068432024675 1107 16-17 1.0486153428293434 1107 18-19 0.8458545891290683 1107 20-21 0.29907096974581293 1107 22-23 0.5609271725627352 1107 24-25 0.41127887037614386 1107 26-27 1.0439308460571937 1107 28-29 0.8838137922633535 1107 30-31 1.2098728617466818 1107 32-33 1.2324491274380591 1107 34-35 1.1431352707217144 1107 36-37 1.3236742133283173 1107 38-39 0.9938313356589177 1107 40-41 0.49978132869861724 1107 42-43 0.27995855561429295 1107 44-45 -0.01755398592886337 1107 46-47 0.46448660101266626 1107 48-49 0.5873767187543848 1107 50-51 -0.7439261533734296 1107 52-53 0.5185047463881176 1107 54-55 0.7932442453721933 1107 56-57 0.8224798477047628 1107 58-59 1.0111625326524987 1107 60-61 1.2429667992788964 1107 62-63 1.255119976816097 1107 64-65 1.2276091279982566 1107 66-67 1.0331213004635984 1107 68-69 0.8795109180246428 1107 70-71 1.3222288775521491 1107 72-73 1.067979716510827 1107 74-75 0.9252256727840305 1107 76-77 0.9951646810783608 1107 78-79 0.9319295492893076 1107 80-81 1.0208854288709333 1107 82-83 0.947170794319689 1107 84-85 1.0150821062562834 1107 86-87 1.1943884783248961 1107 88-89 1.1746472345130705 1107 90-91 1.3308522359691537 1107 92-93 1.314095110069733 1107 94-95 1.2581120111677713 1107 96-97 1.1588998336546226 1107 98-99 1.165468557028209 1107 100-101 1.0394066944793892 1108 1 0.5077133887444489 1108 2 0.49825207829036344 1108 3 0.2683652400808896 1108 4 0.5172833723585128 1108 5 0.5690552182789546 1108 6 0.6685168924830123 1108 7 0.6417403101412162 1108 8 0.7035688355343481 1108 9 0.8311522935622548 1108 10-11 0.6483147540937395 1108 12-13 0.8690056795690566 1108 14-15 0.9968969889311694 1108 16-17 0.820972057298043 1108 18-19 0.6886962546472617 1108 20-21 0.6261585527744771 1108 22-23 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1.685990391159038 2311 84-85 1.7070021779976194 2311 86-87 1.8960299881514224 2311 88-89 1.8844271757841966 2311 90-91 2.125413661316937 2311 92-93 2.264673141999989 2311 94-95 2.348756861565004 2311 96-97 2.4592107221942427 2311 98-99 2.5209304236360097 2311 100-101 2.6344870667134046 2312 1 -0.2131754845039815 2312 2 -0.24690296425819724 2312 3 -0.39450014380761544 2312 4 0.07714852555027818 2312 5 -1.2283617602193289 2312 6 -0.068778168651626 2312 7 0.08399704563893096 2312 8 -2.486141438030284 2312 9 -5.128918270967013 2312 10-11 -0.5890099587409523 2312 12-13 -5.414758809033611 2312 14-15 -2.3901577305116106 2312 16-17 -3.4454585688024864 2312 18-19 -2.4743059832414858 2312 20-21 -3.4018557869179986 2312 22-23 -1.8472351317740419 2312 24-25 -3.749133962927978 2312 26-27 -3.954841144004547 2312 28-29 -5.454718907936545 2312 30-31 -5.633456493826891 2312 32-33 -1.0010400348340056 2312 34-35 0.7707347874145682 2312 36-37 0.8315176832794151 2312 38-39 0.6362338011763313 2312 40-41 -3.2382495761737005 2312 42-43 -4.794016170804671 2312 44-45 -3.8389217590316917 2312 46-47 0.029154810228419592 2312 48-49 -1.5836988317358163 2312 50-51 -6.59791369079332 2312 52-53 -1.5463843605527927 2312 54-55 0.5022431064683488 2312 56-57 0.952329426491751 2312 58-59 1.093721323328161 2312 60-61 1.5029160675018218 2312 62-63 1.2405690061333274 2312 64-65 1.24680375303371 2312 66-67 0.9702520204274379 2312 68-69 0.9977960473658598 2312 70-71 1.3841187909831874 2312 72-73 0.9910424794771657 2312 74-75 0.6048223263435659 2312 76-77 0.8074617746458905 2312 78-79 0.6439862965276184 2312 80-81 0.5901872880134214 2312 82-83 -0.48302539113942444 2312 84-85 -0.16392935508087803 2312 86-87 0.7393850514755265 2312 88-89 0.7632138830650561 2312 90-91 0.9894175852796891 2312 92-93 1.2083480609085164 2312 94-95 1.0799313173621634 2312 96-97 1.1778800971898775 2312 98-99 1.3030511569037273 2312 100-101 1.3239216305537767 2313 1 -0.41270967757993304 2313 2 -0.29481598651115704 2313 3 -0.5191153615231947 2313 4 -1.2190253485801463 2313 5 -2.0822631474696394 2313 6 -1.7651188382745033 2313 7 -2.1310301433824037 2313 8 -3.5427400304390524 2313 9 -2.3297746234343677 2313 10-11 -2.047875545983132 2313 12-13 -3.9620632989512963 2313 14-15 -3.5039532772075717 2313 16-17 -5.931613186867935 2313 18-19 -3.874199871474943 2313 20-21 -4.688320264902352 2313 22-23 -3.5976843269518852 2313 24-25 -0.8385051574820324 2313 26-27 -3.207731248828054 2313 28-29 -4.32456926661466 2313 30-31 -3.681561059683993 2313 32-33 -6.634774762197953 2313 34-35 -2.6364462331568745 2313 36-37 -4.325285335312174 2313 38-39 -1.8123358935743568 2313 40-41 -1.0733864705229905 2313 42-43 -2.6517975873459037 2313 44-45 -4.201178176589963 2313 46-47 -2.3217310365638184 2313 48-49 -1.1564057327298087 2313 50-51 0.2145871567447486 2313 52-53 -0.3667283254036917 2313 54-55 -4.209485464379057 2313 56-57 -5.051131918991885 2313 58-59 -0.9317573661745726 2313 60-61 -7.719706757715489 2313 62-63 -2.020060816358292 2313 64-65 -2.369081822546349 2313 66-67 -3.2259038858958746 2313 68-69 -4.852876219832378 2313 70-71 -4.099098260126752 2313 72-73 -0.7482480294524905 2313 74-75 0.6178049145214288 2313 76-77 -1.6380533928954328 2313 78-79 -0.599004563621822 2313 80-81 0.4534353251152474 2313 82-83 0.9102729558223217 2313 84-85 0.7926597449014885 2313 86-87 0.5362233954539342 2313 88-89 -2.9642907614435714 2313 90-91 0.6227675303592228 2313 92-93 1.5705197246596505 2313 94-95 1.717727447164318 2313 96-97 1.9437036329831088 2313 98-99 1.8736612487090518 2313 100-101 2.404119073022894 2314 1 -28.8116458477927 2314 2 -15.056994038022914 2314 3 -5.473342685151415 2314 4 -3.7841149342464426 2314 5 -1.247016786887336 2314 6 -1.0783887150942135 2314 7 -0.8037625062043503 2314 8 -5.582913602667496 2314 9 -4.2889011631880045 2314 10-11 -1.5259690510223294 2314 12-13 -0.39753362369933143 2314 14-15 -2.8515876557070072 2314 16-17 -2.6523578677189974 2314 18-19 -4.243390800926228 2314 20-21 -3.697698764342441 2314 22-23 -5.9145236326629735 2314 24-25 -2.11776047663097 2314 26-27 -5.236146702355491 2314 28-29 -3.3531246977456775 2314 30-31 -4.15657505744435 2314 32-33 -2.8726666994879864 2314 34-35 -1.8419333552173498 2314 36-37 -7.027902916499187 2314 38-39 -8.939715512835278 2314 40-41 -6.81456788149724 2314 42-43 -3.9936928840984223 2314 44-45 -5.862432375918075 2314 46-47 -6.514690163103573 2314 48-49 -6.721565307197892 2314 50-51 -3.6694413986863808 2314 52-53 -4.195605705022956 2314 54-55 -5.501618723057668 2314 56-57 -2.9646957487791212 2314 58-59 -3.465981890250717 2314 60-61 -1.3140516625307193 2314 62-63 -0.7115501780604205 2314 64-65 -1.9490090341924855 2314 66-67 -1.1685830572284601 2314 68-69 0.021073948140745813 2314 70-71 0.38389446103786184 2314 72-73 -0.06991656248720801 2314 74-75 -0.7605545479645777 2314 76-77 -2.4260573122683375 2314 78-79 -4.176342189601662 2314 80-81 -3.3237203299799383 2314 82-83 -3.131370381243748 2314 84-85 -1.4357837041466617 2314 86-87 -0.4672060558338593 2314 88-89 -0.7581037513651872 2314 90-91 -0.7212974192488417 2314 92-93 -0.08247579605703237 2314 94-95 0.10196596900082255 2314 96-97 0.26215268113540446 2314 98-99 0.7491511703215927 2314 100-101 0.5005636418918726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 48.0 5 96.0 6 202.0 7 562.0 8 1458.0 9 2462.0 10 3868.0 11 6090.0 12 8412.0 13 10346.0 14 13053.0 15 16542.0 16 19266.0 17 22086.0 18 19652.0 19 15063.0 20 14065.0 21 14495.0 22 15992.0 23 18050.0 24 21700.0 25 26972.0 26 34157.0 27 43175.0 28 55322.0 29 69818.0 30 86962.0 31 106818.0 32 130919.0 33 159751.0 34 198347.0 35 244651.0 36 310859.0 37 397733.0 38 375131.0 39 84310.0 40 64.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.01048390614311 22.084615496455 15.31762345408556 22.587277143316335 2 14.811005773161412 22.174307646368458 40.666434636300245 22.348251944169885 3 17.427677992990976 27.626863451954126 31.666999551488388 23.278459003566514 4 11.96173450369039 18.527178625942216 40.319245362976794 29.191841507390592 5 11.371703465946768 39.37300618792941 36.64403906596404 12.61125128015978 6 28.184901258261174 40.57300775313643 18.446021745195342 12.796069243407048 7 25.42098012294994 34.08853070236534 23.545928867651266 16.944560307033463 8 24.62196642204074 36.4553973938569 21.038745278136265 17.883890905966098 9 25.457001071603603 16.443759252930235 22.10473777494912 35.994501900517044 10-11 23.101336973620345 27.841073848045745 29.698840541381514 19.35874863695239 12-13 23.921186321299796 25.653723322230142 29.54445663890019 20.880633717569864 14-15 21.653965816198202 27.151888725874695 27.431992802088427 23.76215265583867 16-17 20.445478684270107 30.130817255682423 28.186647371785238 21.237056688262232 18-19 20.30520102475283 28.892822767123 30.65009964618639 20.151876561937787 20-21 21.208236005488683 28.153745884371183 30.588946434338087 20.049071675802043 22-23 21.00982649909732 27.854826944454363 30.37839261029061 20.756953946157708 24-25 20.773644759826958 28.2554237275497 30.557566923611578 20.413364589011767 26-27 20.598390781684234 28.365769107309212 30.492152078351285 20.543688032655265 28-29 20.545641546438922 28.055967803581755 30.828380470875754 20.570010179103566 30-31 21.067725347318277 27.909596206328935 30.59257471683824 20.43010372951455 32-33 20.774424957263818 28.32689388536599 30.277896530271224 20.620784627098967 34-35 20.941275676710745 28.203151149298773 30.46456948432136 20.391003689669123 36-37 21.041447418502464 27.813047139434378 30.340453650447603 20.805051791615558 38-39 20.649755395090637 28.464830748313467 30.355984261314756 20.52942959528114 40-41 20.845809837089657 28.082149316233313 30.63110344808643 20.4409373985906 42-43 20.746539699381355 28.173266553605718 30.157333121965348 20.922860625047576 44-45 20.8443831360375 28.26779051751825 30.131632358570208 20.756193987874042 46-47 21.016726012941106 28.12554155969585 29.766662473207823 21.091069954155223 48-49 20.827714635563023 28.402124770411767 29.774728811164657 20.995431782860553 50-51 20.9102028465635 28.304540626748224 29.4846884746244 21.300568052063877 52-53 21.001446350065372 28.582463142793912 29.30034090855583 21.115749598584884 54-55 21.12093727165691 28.43659110495534 29.365811935281716 21.076659688106034 56-57 21.149703432461237 28.604955401076403 29.038564766928836 21.206776399533528 58-59 20.951056167739036 28.41953986342853 29.427669210991247 21.201734757841187 60-61 21.246001218396014 28.77154878538082 29.03498523111756 20.947464765105607 62-63 21.102317279920797 28.55909329646896 29.237429714368453 21.10115970924179 64-65 20.944196475872577 28.304467907930974 29.2755219047335 21.475813711462948 66-67 21.137704601533216 28.496924306065686 28.895022225989337 21.470348866411758 68-69 21.023018113562777 28.44798376424567 29.045935577691456 21.483062544500097 70-71 21.238582628191534 28.101847282835717 29.191075295547396 21.468494793425354 72-73 21.261304920650957 28.312573514480206 28.94930824039076 21.476813324478076 74-75 20.936932383802624 28.68576531879504 28.780095798016625 21.597206499385713 76-77 20.809356971132623 28.60235972528337 28.92317375163674 21.66510955194727 78-79 20.836533724619795 28.638994489539304 28.62163144715759 21.90284033868331 80-81 20.98480758153398 28.720882963679138 28.45402094087992 21.840288513906962 82-83 21.15555982821301 28.52374235090769 28.667414817706856 21.65328300317245 84-85 20.968438102801787 28.552420310486575 28.4155759665625 22.063565620149138 86-87 20.704798797083114 28.83771138156511 28.57357271337492 21.883917107976856 88-89 21.0431230514773 28.532160312038314 28.47357464767178 21.951141988812612 90-91 20.947703089393567 28.39438449034767 28.74671249795221 21.911199922306555 92-93 21.175524676388736 28.5259972029816 28.446752272381246 21.85172584824841 94-95 20.82435744404342 28.35660982525366 28.510406538135857 22.30862619256706 96-97 20.946010636836856 28.345220054761583 28.447476203667883 22.26129310473368 98-99 20.659593127627808 28.465272232500737 28.31753624240474 22.55759839746671 100-101 20.64206539550594 28.501780702800783 28.388514977052555 22.46763892464072 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 383.0 1 279.5 2 228.5 3 290.0 4 263.0 5 259.0 6 420.5 7 863.0 8 1471.5 9 1924.0 10 1852.0 11 1684.0 12 1791.5 13 1935.5 14 2203.0 15 2508.5 16 2768.5 17 2995.5 18 3359.0 19 3851.5 20 4425.0 21 5143.0 22 6003.0 23 7547.5 24 9497.0 25 12329.5 26 17452.5 27 23326.5 28 27425.0 29 33442.5 30 42308.0 31 49991.5 32 60043.0 33 71345.0 34 82201.5 35 91449.0 36 101583.5 37 110781.0 38 109589.0 39 105287.5 40 101605.0 41 96940.5 42 91882.0 43 90669.0 44 92045.5 45 91087.5 46 85867.0 47 79645.0 48 78037.5 49 76186.0 50 72519.5 51 67969.5 52 63144.5 53 59191.0 54 56130.5 55 52895.0 56 48015.5 57 42579.0 58 38634.0 59 34356.5 60 28603.5 61 23449.5 62 18521.5 63 13674.5 64 10009.5 65 7338.0 66 5578.5 67 4397.5 68 3536.5 69 2859.0 70 2210.0 71 1648.5 72 1220.0 73 903.5 74 679.5 75 518.0 76 414.0 77 321.5 78 221.0 79 150.0 80 123.0 81 99.5 82 76.5 83 74.0 84 60.5 85 42.0 86 24.0 87 13.5 88 12.5 89 11.5 90 7.0 91 3.5 92 2.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14376988656540254 2 0.0 3 0.003452988541963817 4 2.7466954311075814E-4 5 2.7466954311075814E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.019619253079339868 26-27 0.007808462725577266 28-29 0.006238922479230078 30-31 0.0024327873818381436 32-33 0.0012752514501570913 34-35 0.0019619253079339867 36-37 0.0013733477155537907 38-39 7.651508700942549E-4 40-41 0.0023543103695207843 42-43 0.0015303017401885098 44-45 6.866738577768954E-4 46-47 0.035255797783573746 48-49 0.018716767437690233 50-51 0.08567727819747721 52-53 0.0012948707032364312 54-55 0.037374677116142445 56-57 9.809626539669933E-4 58-59 0.0013537284624744509 60-61 0.0033156537704084376 62-63 0.0030017457211389993 64-65 0.010005819070463332 66-67 0.00584653741764328 68-69 0.002648599165710882 70-71 0.00598387218919866 72-73 0.004885194016755627 74-75 0.0011771551847603921 76-77 0.0 78-79 1.9619253079339867E-5 80-81 3.531465554281176E-4 82-83 4.7086207390415683E-4 84-85 3.9238506158679734E-5 86-87 0.0017657327771405879 88-89 0.00439471268977213 90-91 0.0015499209932678495 92-93 0.0034922270481224965 94-95 9.024856416496339E-4 96-97 5.885775923801961E-4 98-99 0.002040402320251346 100-101 0.0033352730234877774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2548517.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.225171526420006 #Duplication Level Percentage of deduplicated Percentage of total 1 82.75404721232832 45.7010645180629 2 10.672849749623703 11.788199161973555 3 2.8007335508026086 4.6401297222862015 4 1.1046076183985458 2.4400858078179994 5 0.5716541990367052 1.578485059780014 6 0.3404433770540632 1.128062633570659 7 0.23585973141647823 0.9117775886555253 8 0.17249673322071113 0.7620929343888707 9 0.12612741741300648 0.6268867434735891 >10 0.9758056050726817 10.99698659810032 >50 0.13312156992771265 5.125295726617248 >100 0.10510852664932684 10.679080243499717 >500 0.0060621773808778295 2.095394060388013 >1k 8.660253401250221E-4 0.6151271883598067 >5k 2.1650633503125552E-4 0.9113320130254853 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9766 0.3832032511456663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8056 0.31610540561432393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5044 0.19791902506438055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.9238506158679734E-5 0.0 3 0.0 0.0 0.0 3.9238506158679734E-5 0.0 4 0.0 0.0 0.0 3.9238506158679734E-5 0.0 5 0.0 0.0 0.0 3.9238506158679734E-5 0.0 6 0.0 0.0 0.0 3.9238506158679734E-5 0.0 7 0.0 0.0 0.0 3.9238506158679734E-5 0.0 8 0.0 0.0 0.0 3.9238506158679734E-5 0.0 9 0.0 0.0 0.0 3.9238506158679734E-5 0.0 10-11 7.847701231735947E-5 0.0 0.0 5.8857759238019604E-5 0.0 12-13 7.847701231735947E-5 0.0 0.0 7.847701231735947E-5 0.0 14-15 7.847701231735947E-5 0.0 0.0 7.847701231735947E-5 0.0 16-17 7.847701231735947E-5 0.0 0.0 1.1771551847603921E-4 0.0 18-19 1.1771551847603921E-4 0.0 0.0 1.765732777140588E-4 1.9619253079339867E-5 20-21 1.1771551847603921E-4 0.0 0.0 2.550502900314183E-4 3.9238506158679734E-5 22-23 1.1771551847603921E-4 0.0 0.0 3.531465554281176E-4 3.9238506158679734E-5 24-25 1.1771551847603921E-4 0.0 0.0 6.474353516182156E-4 7.847701231735947E-5 26-27 1.1771551847603921E-4 0.0 0.0 0.0014125862217124704 7.847701231735947E-5 28-29 1.1771551847603921E-4 0.0 0.0 0.003688419578915895 7.847701231735947E-5 30-31 1.1771551847603921E-4 0.0 0.0 0.010398204132050129 7.847701231735947E-5 32-33 1.1771551847603921E-4 0.0 0.0 0.027231523274123735 7.847701231735947E-5 34-35 1.1771551847603921E-4 0.0 0.0 0.05569905949224588 7.847701231735947E-5 36-37 1.1771551847603921E-4 0.0 0.0 0.10119610738323503 7.847701231735947E-5 38-39 1.1771551847603921E-4 0.0 0.0 0.16240817699077542 7.847701231735947E-5 40-41 1.1771551847603921E-4 0.0 0.0 0.22615113024555064 7.847701231735947E-5 42-43 1.1771551847603921E-4 0.0 0.0 0.29477927751708155 7.847701231735947E-5 44-45 1.1771551847603921E-4 0.0 0.0 0.3771801404503089 7.847701231735947E-5 46-47 1.1771551847603921E-4 0.0 0.0 0.4753548828593256 7.847701231735947E-5 48-49 1.9619253079339866E-4 0.0 0.0 0.5792388279144303 7.847701231735947E-5 50-51 1.9619253079339866E-4 0.0 0.0 0.6841625933827398 7.847701231735947E-5 52-53 1.9619253079339866E-4 0.0 0.0 0.7880857769440031 7.847701231735947E-5 54-55 1.9619253079339866E-4 1.9619253079339867E-5 0.0 0.900935720656366 7.847701231735947E-5 56-57 1.9619253079339866E-4 3.9238506158679734E-5 0.0 1.0362889476507318 7.847701231735947E-5 58-59 1.9619253079339866E-4 3.9238506158679734E-5 0.0 1.183040960684194 7.847701231735947E-5 60-61 1.9619253079339866E-4 3.9238506158679734E-5 0.0 1.3323630958710497 7.847701231735947E-5 62-63 1.9619253079339866E-4 3.9238506158679734E-5 0.0 1.48333324831657 7.847701231735947E-5 64-65 1.9619253079339866E-4 3.9238506158679734E-5 0.0 1.642778918092365 7.847701231735947E-5 66-67 1.9619253079339866E-4 3.9238506158679734E-5 0.0 1.8237861470023549 7.847701231735947E-5 68-69 1.9619253079339866E-4 3.9238506158679734E-5 0.0 2.0222348918998776 9.809626539669935E-5 70-71 1.9619253079339866E-4 3.9238506158679734E-5 0.0 2.242029384147722 1.1771551847603921E-4 72-73 2.3543103695207842E-4 3.9238506158679734E-5 0.0 2.4662578275914973 1.1771551847603921E-4 74-75 2.3543103695207842E-4 3.9238506158679734E-5 0.0 2.695096795508918 1.1771551847603921E-4 76-77 3.1390804926943787E-4 3.9238506158679734E-5 0.0 2.9412399446423154 1.1771551847603921E-4 78-79 3.1390804926943787E-4 3.9238506158679734E-5 0.0 3.2116521098348567 1.1771551847603921E-4 80-81 3.1390804926943787E-4 3.9238506158679734E-5 0.0 3.5060193830372723 1.3733477155537907E-4 82-83 3.1390804926943787E-4 3.9238506158679734E-5 0.0 3.8086463617860895 1.5695402463471894E-4 84-85 3.1390804926943787E-4 3.9238506158679734E-5 0.0 4.114745948329951 1.5695402463471894E-4 86-87 3.1390804926943787E-4 3.9238506158679734E-5 0.0 4.428124277766246 1.5695402463471894E-4 88-89 3.1390804926943787E-4 3.9238506158679734E-5 0.0 4.772579504080216 1.5695402463471894E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4155 0.0 60.09874 1 GTATCAA 7960 0.0 42.843323 1 TCAACGC 10265 0.0 32.522408 4 CAACGCA 10350 0.0 32.301197 5 ATCAACG 10380 0.0 32.07311 3 AACGCAG 10825 0.0 31.103168 6 TATCAAC 10895 0.0 30.94935 2 ACGCAGA 12390 0.0 26.944511 7 CGCAGAG 12425 0.0 26.601072 8 ACGTGCG 145 7.9109304E-7 26.200424 9 GTGGTAT 1965 0.0 24.931688 1 GCAGAGT 13350 0.0 24.18878 9 GTACATG 9035 0.0 23.216003 1 TGGTATC 2080 0.0 22.83269 2 TACATGG 9480 0.0 21.541729 2 ACATGGG 8930 0.0 21.538921 3 GAGTACT 8705 0.0 21.493828 12-13 CAGAGTA 13370 0.0 21.098003 10-11 AGAGTAC 12745 0.0 21.070705 10-11 GTACTTT 9260 0.0 20.128668 14-15 >>END_MODULE