FastQCFastQC Report
Sat 18 Jun 2016
SRR1931150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310432
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58680.25397847675239954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46300.20039542388609577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.11560608578828548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39750.047.2699741
GTATCAA68350.038.711231
ATCAACG76300.032.821443
CAACGCA77600.032.6751485
TCAACGC78350.032.458914
AACGCAG80300.031.6784616
TATCAAC85150.030.3987032
ACGCAGA87200.028.4722147
CGCAGAG88600.028.1284588
GCAGAGT100500.023.8981489
GAGTACT62750.023.26622212-13
CAGAGTA97950.021.4127810-11
GTACATG57600.021.1372091
GTACTTT71650.020.86177414-15
GTGGTAT21700.020.763691
GTATAGG7250.020.4955751
AGAGTAC86750.020.47670410-11
TACATGG57200.020.3636762
GTATACG3757.2759576E-1219.1732791
ACATGGG57950.018.6850953
Produced by FastQC (version 0.11.5)