##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931150_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310432 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.770474093156604 31.0 28.0 31.0 23.0 34.0 2 28.51466868533677 31.0 28.0 33.0 16.0 34.0 3 28.547752541516047 31.0 28.0 34.0 16.0 34.0 4 31.75073968850847 35.0 32.0 37.0 19.0 37.0 5 31.818092893450228 35.0 32.0 37.0 19.0 37.0 6 32.113005273472666 35.0 32.0 37.0 22.0 37.0 7 31.844330410936138 35.0 32.0 37.0 18.0 37.0 8 31.969447705017934 35.0 32.0 37.0 19.0 37.0 9 32.978692729325076 37.0 32.0 39.0 18.0 39.0 10-11 32.984044325909615 37.0 32.0 39.0 19.0 39.0 12-13 33.026871381629064 37.0 32.0 39.0 19.5 39.0 14-15 33.773427220537116 38.0 32.0 40.0 18.0 41.0 16-17 33.75826274047451 38.0 32.0 40.0 18.5 41.0 18-19 33.789826534604785 38.0 32.0 40.0 18.5 41.0 20-21 34.00864665136217 38.0 32.0 40.0 22.0 41.0 22-23 33.9058611116882 38.0 32.0 40.0 21.5 41.0 24-25 33.77399572893727 38.0 32.0 40.0 20.0 41.0 26-27 33.438112006758914 38.0 31.5 40.0 18.0 41.0 28-29 33.35338239775072 37.5 31.5 40.0 18.0 41.0 30-31 33.076513396628854 37.0 31.0 40.0 16.5 41.0 32-33 32.87030369212338 37.0 31.0 40.0 16.0 41.0 34-35 32.83051243230703 37.0 31.0 40.0 16.0 41.0 36-37 32.46998613246354 37.0 30.0 40.0 15.0 41.0 38-39 32.28793987444772 37.0 30.0 40.0 14.0 41.0 40-41 32.16801165323194 36.5 30.0 40.0 9.0 41.0 42-43 32.35572113786513 37.0 30.0 40.0 9.0 41.0 44-45 32.38604490415645 37.0 30.0 40.0 10.5 41.0 46-47 32.13375247572748 37.0 30.0 40.0 8.5 41.0 48-49 31.908872886109613 37.0 30.0 40.0 8.0 41.0 50-51 31.393702779393635 36.5 29.5 39.5 6.5 40.5 52-53 31.31713116854337 36.0 30.0 39.5 2.0 40.5 54-55 31.31859756097561 36.0 29.5 40.0 2.0 41.0 56-57 31.06466128412349 36.0 29.0 40.0 2.0 41.0 58-59 30.912135046606004 35.5 29.0 40.0 2.0 41.0 60-61 30.751904405756154 35.0 29.0 39.0 2.0 41.0 62-63 30.592699979917178 35.0 29.0 39.0 2.0 41.0 64-65 30.26523979065387 35.0 28.0 39.0 2.0 40.0 66-67 30.04637942168391 35.0 28.0 39.0 2.0 40.0 68-69 29.830204697649616 34.5 28.0 38.5 2.0 40.0 70-71 29.520744172518384 34.0 28.0 38.0 2.0 40.0 72-73 29.18744697961247 34.0 28.0 37.0 2.0 40.0 74-75 28.774807698300577 34.0 27.5 37.0 2.0 39.0 76-77 28.466055698674534 34.0 27.0 36.0 2.0 39.0 78-79 28.05178988171909 33.5 26.0 36.0 2.0 39.0 80-81 27.64810498642678 33.0 26.0 35.5 2.0 37.5 82-83 27.304326636750183 33.0 26.0 35.0 2.0 37.0 84-85 26.98683795930804 33.0 26.0 35.0 2.0 36.5 86-87 26.664485472846636 33.0 25.5 35.0 2.0 36.0 88-89 26.324357306339245 32.5 25.0 35.0 2.0 36.0 90-91 25.949189588786858 32.0 24.0 35.0 2.0 35.5 92-93 25.59497033455215 32.0 22.5 35.0 2.0 35.0 94-95 25.29887332758549 32.0 20.0 35.0 2.0 35.0 96-97 24.90565444038171 32.0 18.5 35.0 2.0 35.0 98-99 24.549402665821802 32.0 8.5 34.5 2.0 35.0 100-101 23.963367456821928 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.228147953428902 1101 2 5.863804169851509 1101 3 5.341036498560793 1101 4 6.658047680608021 1101 5 6.118232828899526 1101 6 5.632228724399788 1101 7 6.187515777119948 1101 8 5.630285940866369 1101 9 6.365248047906885 1101 10-11 6.28472988558325 1101 12-13 6.250305923435825 1101 14-15 6.320189761529971 1101 16-17 6.380041466497854 1101 18-19 6.4163752179897635 1101 20-21 5.7990258826601355 1101 22-23 5.797643188786317 1101 24-25 5.789378254958891 1101 26-27 5.906089353817531 1101 28-29 5.781685656235311 1101 30-31 5.871764415379424 1101 32-33 5.777519769947073 1101 34-35 5.679462604497296 1101 36-37 5.663996358003011 1101 38-39 5.668734753993331 1101 40-41 5.677606217277422 1101 42-43 5.689690117491047 1101 44-45 5.6621537483108355 1101 46-47 5.662320893892847 1101 48-49 5.380322362610336 1101 50-51 5.263342287097618 1101 52-53 5.052276237274395 1101 54-55 5.197130701135642 1101 56-57 5.235724861149848 1101 58-59 5.153640922416336 1101 60-61 5.146934581789306 1101 62-63 5.164305311077467 1101 64-65 5.125315570672299 1101 66-67 4.9127313101788666 1101 68-69 5.008095594858755 1101 70-71 4.773516773284566 1101 72-73 4.604440049046179 1101 74-75 4.7969299926109095 1101 76-77 4.596980265259717 1101 78-79 4.5246591044614135 1101 80-81 4.550725298068471 1101 82-83 4.3566956126564484 1101 84-85 4.21945245845313 1101 86-87 4.048673193615489 1101 88-89 4.0943572976157725 1101 90-91 4.140194113415941 1101 92-93 4.05471159822363 1101 94-95 3.916766926512942 1101 96-97 3.8611060573625586 1101 98-99 3.607051855449331 1101 100-101 3.5415949589098332 1102 1 5.384416909994695 1102 2 5.819126177943968 1102 3 5.579776182716046 1102 4 6.935369330291152 1102 5 6.443383659492309 1102 6 5.9426323308126285 1102 7 6.413937861061825 1102 8 6.0476395973361186 1102 9 6.755999651719176 1102 10-11 6.7512208128261655 1102 12-13 6.746832588580567 1102 14-15 7.101313751014775 1102 16-17 7.006053776052379 1102 18-19 7.037979879996808 1102 20-21 6.2205984024351615 1102 22-23 6.443526291546064 1102 24-25 6.264657214571628 1102 26-27 6.451826308690368 1102 28-29 6.338363704173261 1102 30-31 6.565490822413878 1102 32-33 6.375001521864842 1102 34-35 6.364706310107103 1102 36-37 6.468946695852431 1102 38-39 6.279149253919709 1102 40-41 6.600816902691271 1102 42-43 6.559755651062517 1102 44-45 6.468887913471782 1102 46-47 6.473597746808885 1102 48-49 6.130992933036843 1102 50-51 6.192140760338706 1102 52-53 6.013084159741286 1102 54-55 6.339966224825389 1102 56-57 6.094322598540465 1102 58-59 6.263787049465492 1102 60-61 6.1599887097016115 1102 62-63 5.995352530812017 1102 64-65 5.973272336505058 1102 66-67 5.937600327544583 1102 68-69 5.795686580586281 1102 70-71 5.813878861634784 1102 72-73 5.633076010976996 1102 74-75 5.666810475370681 1102 76-77 5.536220072820768 1102 78-79 5.395364820071485 1102 80-81 5.478620454772994 1102 82-83 5.414769037484486 1102 84-85 5.30148203347413 1102 86-87 5.310307257690184 1102 88-89 5.255149695957641 1102 90-91 5.2948215760909 1102 92-93 5.422947287781881 1102 94-95 5.3912585085243165 1102 96-97 5.323813803958824 1102 98-99 5.035425556910102 1102 100-101 5.001762844044734 1103 1 5.060651646097849 1103 2 5.631924759163599 1103 3 5.31434071135952 1103 4 6.669258876022802 1103 5 6.3779297144330975 1103 6 5.671018791435934 1103 7 6.309905975450988 1103 8 5.61232757649778 1103 9 6.2911043052448825 1103 10-11 6.333523929339808 1103 12-13 6.3858066745941215 1103 14-15 6.517369130557004 1103 16-17 6.381308020385571 1103 18-19 6.330334769062215 1103 20-21 5.762657827718275 1103 22-23 5.955629453320277 1103 24-25 5.70993695261452 1103 26-27 5.875473340890036 1103 28-29 5.793478841525236 1103 30-31 5.930368965252548 1103 32-33 5.794662275407173 1103 34-35 5.711982709028241 1103 36-37 5.736983380007537 1103 38-39 5.596546863422585 1103 40-41 5.795580993507997 1103 42-43 5.710952631535811 1103 44-45 5.5644296317108655 1103 46-47 5.538706118167262 1103 48-49 5.376465259430226 1103 50-51 5.316975305239044 1103 52-53 5.204759793851128 1103 54-55 5.446814772792358 1103 56-57 5.5382241983631175 1103 58-59 5.490244740067922 1103 60-61 5.24564214765768 1103 62-63 5.341927737255649 1103 64-65 5.1856575686271285 1103 66-67 5.350227680632461 1103 68-69 5.357420499228095 1103 70-71 5.093671171032359 1103 72-73 5.030374578341352 1103 74-75 5.052431122504483 1103 76-77 5.101053878772994 1103 78-79 5.021742842831085 1103 80-81 5.127465271052021 1103 82-83 4.944392081581217 1103 84-85 4.888832973785597 1103 86-87 4.829469105799404 1103 88-89 4.868514611625191 1103 90-91 4.822734417161634 1103 92-93 4.725126376474126 1103 94-95 4.694300665513843 1103 96-97 4.4701101729525625 1103 98-99 4.370855809445619 1103 100-101 4.406032347656346 1104 1 4.647530349404473 1104 2 5.444606991599347 1104 3 5.032971136233616 1104 4 6.438586909001458 1104 5 6.089555880013425 1104 6 5.675512941362179 1104 7 6.166227557743824 1104 8 5.552897293525302 1104 9 6.246425503595052 1104 10-11 6.200554649243525 1104 12-13 6.166618410217048 1104 14-15 6.2098384095306365 1104 16-17 6.093606590816602 1104 18-19 6.28801328556165 1104 20-21 5.7309247419643405 1104 22-23 5.811498836474847 1104 24-25 5.5539891530999554 1104 26-27 5.788748607573009 1104 28-29 5.595643739286473 1104 30-31 5.811270680420968 1104 32-33 5.668170287684358 1104 34-35 5.647891115127315 1104 36-37 5.67210127959418 1104 38-39 5.456258311425291 1104 40-41 5.7601541996986825 1104 42-43 5.719138300370666 1104 44-45 5.649456485244599 1104 46-47 5.6756094072760845 1104 48-49 5.365766450101539 1104 50-51 5.3240649412569105 1104 52-53 5.153433563831303 1104 54-55 5.487757935806329 1104 56-57 5.32485485450092 1104 58-59 5.317752267364902 1104 60-61 5.1764891902887875 1104 62-63 5.114732808891265 1104 64-65 5.140531395188329 1104 66-67 5.082526520496938 1104 68-69 5.0342263769187845 1104 70-71 5.093486496855981 1104 72-73 4.982459095123687 1104 74-75 4.9054473177000375 1104 76-77 4.955797647563166 1104 78-79 4.9898020687936935 1104 80-81 4.913687567118966 1104 82-83 4.581176763840595 1104 84-85 4.603309408462582 1104 86-87 4.58958661246227 1104 88-89 4.597725631902303 1104 90-91 4.585124314305414 1104 92-93 4.538352247024825 1104 94-95 4.367674859178113 1104 96-97 4.319976410471622 1104 98-99 4.211455444812351 1104 100-101 4.169914793842629 1105 1 4.551268932824975 1105 2 5.257853725367166 1105 3 4.921506499989842 1105 4 6.39228976003767 1105 5 3.8051324424367436 1105 6 5.015414351964218 1105 7 5.685215349730619 1105 8 1.1242091793404825 1105 9 0.6350495090299049 1105 10-11 2.213324220581743 1105 12-13 5.4155993755538745 1105 14-15 3.5874697439642453 1105 16-17 5.696953138147396 1105 18-19 6.172118288591729 1105 20-21 3.4719675899456526 1105 22-23 5.541389100006704 1105 24-25 5.580304772067009 1105 26-27 5.796053688243834 1105 28-29 3.4832247919691284 1105 30-31 5.636819721188424 1105 32-33 5.743680177228274 1105 34-35 5.742208618315935 1105 36-37 5.913072304380215 1105 38-39 5.902302686855744 1105 40-41 5.99159665760147 1105 42-43 3.0272571199226803 1105 44-45 5.209702291514418 1105 46-47 5.506180212815259 1105 48-49 5.410443579335315 1105 50-51 5.5808483732311025 1105 52-53 5.559001777669774 1105 54-55 5.822911709129571 1105 56-57 5.660662618684832 1105 58-59 5.605354742935361 1105 60-61 5.499709836107524 1105 62-63 5.394242492201979 1105 64-65 5.474741196669566 1105 66-67 5.413274954034424 1105 68-69 5.540697701956102 1105 70-71 5.276622272747762 1105 72-73 5.236761800804086 1105 74-75 5.302339055192739 1105 76-77 5.251539235150798 1105 78-79 5.279288145570895 1105 80-81 5.16342929311444 1105 82-83 5.114093361808813 1105 84-85 5.022382864429311 1105 86-87 5.040792401218809 1105 88-89 5.120387021792794 1105 90-91 5.042471399827679 1105 92-93 4.961803885062388 1105 94-95 4.99877907256662 1105 96-97 4.9656899580632405 1105 98-99 4.838052718232916 1105 100-101 4.889489468184404 1106 1 4.29550531657279 1106 2 4.621158639770243 1106 3 4.588894589385998 1106 4 6.145082030983232 1106 5 6.1425105577171415 1106 6 5.2255529893776185 1106 7 5.8052280977128845 1106 8 5.379101497096986 1106 9 6.109377188086402 1106 10-11 6.025442794522366 1106 12-13 6.239125475525519 1106 14-15 6.003234282767782 1106 16-17 5.83460952535555 1106 18-19 6.08042355710543 1106 20-21 5.39801248030027 1106 22-23 5.459871864914831 1106 24-25 5.410949699137305 1106 26-27 5.706111571351915 1106 28-29 5.622635152748828 1106 30-31 5.661234794124347 1106 32-33 5.636708251677231 1106 34-35 5.217346480724075 1106 36-37 5.434450296343144 1106 38-39 5.2985551869854355 1106 40-41 5.4918123078619345 1106 42-43 2.4631229110663746 1106 44-45 4.744517616614374 1106 46-47 4.891287042397664 1106 48-49 4.705145346751117 1106 50-51 5.076830809655046 1106 52-53 4.80708437738841 1106 54-55 5.1227127082806305 1106 56-57 4.990810276600307 1106 58-59 4.992440631534027 1106 60-61 4.785408535185869 1106 62-63 4.944224274142524 1106 64-65 4.961758344320959 1106 66-67 5.107582827746153 1106 68-69 5.102723554826866 1106 70-71 5.009219133650316 1106 72-73 4.7312190765814 1106 74-75 4.844452087612808 1106 76-77 4.699840094366046 1106 78-79 4.938155603519483 1106 80-81 4.918208738111279 1106 82-83 4.928271834492438 1106 84-85 4.881347205800644 1106 86-87 4.936182183822844 1106 88-89 5.044232504692513 1106 90-91 5.0453288240208245 1106 92-93 5.04894810096053 1106 94-95 4.886448583611298 1106 96-97 4.930039793883271 1106 98-99 4.842713699249792 1106 100-101 4.887666186777977 1107 1 3.302575806541441 1107 2 0.024550226825958532 1107 3 1.1130916243258788 1107 4 2.9885723998493603 1107 5 3.070997504979985 1107 6 4.264835959675139 1107 7 5.175152513631616 1107 8 4.659229825748778 1107 9 5.158623570698236 1107 10-11 2.9490769633692295 1107 12-13 5.085695441754169 1107 14-15 3.603242373562562 1107 16-17 2.2380665799109813 1107 18-19 2.9289839514915315 1107 20-21 2.820091796029022 1107 22-23 4.604354467853213 1107 24-25 4.336974358150449 1107 26-27 3.6282597514789465 1107 28-29 4.526265140322359 1107 30-31 4.045929079429236 1107 32-33 4.545034914737013 1107 34-35 4.892508018691522 1107 36-37 5.007975085056053 1107 38-39 4.997283944017017 1107 40-41 3.8542403862549435 1107 42-43 4.34775188327604 1107 44-45 4.941691366123365 1107 46-47 5.1607395364623585 1107 48-49 2.8029692317654806 1107 50-51 4.295722938979299 1107 52-53 4.45177681601572 1107 54-55 4.85952270553841 1107 56-57 3.713722789786445 1107 58-59 2.940959089663238 1107 60-61 3.874320656802251 1107 62-63 4.522215675665816 1107 64-65 4.641280882434163 1107 66-67 4.608799689456564 1107 68-69 4.663446822470359 1107 70-71 4.430807288726761 1107 72-73 4.449082563256912 1107 74-75 4.50947419215823 1107 76-77 4.501969826245755 1107 78-79 4.3689718967743545 1107 80-81 4.374085804969653 1107 82-83 4.249323217833062 1107 84-85 4.232837579607715 1107 86-87 4.133536277765003 1107 88-89 4.231160127283495 1107 90-91 4.267705090446373 1107 92-93 4.267474600475079 1107 94-95 4.325159114738007 1107 96-97 4.442132598387435 1107 98-99 4.381034266420681 1107 100-101 4.6018610229980546 1108 1 -1.7803624906329532 1108 2 -1.330894947300834 1108 3 -2.021813491955186 1108 4 -4.196225997708169 1108 5 -8.92212741863921 1108 6 -7.214106522631315 1108 7 -6.004828265206022 1108 8 -6.287241702703476 1108 9 -7.902379231539268 1108 10-11 -3.439691279582835 1108 12-13 -2.073038743399337 1108 14-15 -6.881021840771091 1108 16-17 -5.969472661062152 1108 18-19 -4.444468064211289 1108 20-21 -4.730057293940593 1108 22-23 -3.526066559219359 1108 24-25 -8.278705173723658 1108 26-27 -9.58259106635846 1108 28-29 -6.522097576840331 1108 30-31 -6.416099074803171 1108 32-33 -2.8093417321403216 1108 34-35 -1.1293557854906098 1108 36-37 -4.616670749215292 1108 38-39 -5.7455204482276265 1108 40-41 -3.5223861228689444 1108 42-43 -4.379165569710906 1108 44-45 -1.8838220468818463 1108 46-47 -2.3017368524890642 1108 48-49 -1.415415011383832 1108 50-51 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-2.709016088360354 2313 38-39 -4.264683864913412 2313 40-41 -3.073796996922624 2313 42-43 -5.562372727315861 2313 44-45 -5.826545913212044 2313 46-47 -7.66937468991355 2313 48-49 -4.797498057954144 2313 50-51 -2.4742926373446856 2313 52-53 -2.2423561019489746 2313 54-55 -5.2759523723432125 2313 56-57 -3.044324977607115 2313 58-59 -4.036403319474871 2313 60-61 -2.861362105399202 2313 62-63 -4.652304054344565 2313 64-65 -3.1108868226541055 2313 66-67 -3.7351125639183103 2313 68-69 -4.669652866003165 2313 70-71 -5.593569658313736 2313 72-73 -7.783368111717127 2313 74-75 -7.733347718537516 2313 76-77 -6.130299124948433 2313 78-79 -7.505742537285876 2313 80-81 -8.952731432828894 2313 82-83 -7.792888620173169 2313 84-85 -9.247769897027798 2313 86-87 -7.8397892674063385 2313 88-89 -9.120812873754927 2313 90-91 -9.191965210695887 2313 92-93 -7.768480747396156 2313 94-95 -7.824957707691901 2313 96-97 -7.778441129546106 2313 98-99 -7.653506232021687 2313 100-101 -7.291389236607344 2314 1 -13.06174127486831 2314 2 -15.093745385530006 2314 3 -14.085947522917298 2314 4 -24.99078897086715 2314 5 -19.167925476666227 2314 6 -17.677381508327713 2314 7 -17.774088375157184 2314 8 -13.113388685680118 2314 9 -14.644532980741166 2314 10-11 -13.515264045526166 2314 12-13 -14.078411922281415 2314 14-15 -17.102416711375323 2314 16-17 -16.650168603814578 2314 18-19 -15.358403917742415 2314 20-21 -16.32203981452534 2314 22-23 -16.981650768043647 2314 24-25 -16.678163295266955 2314 26-27 -16.660087563012663 2314 28-29 -17.644351907307414 2314 30-31 -18.106589874892727 2314 32-33 -15.369609644809582 2314 34-35 -16.18797982051578 2314 36-37 -15.99469212584362 2314 38-39 -15.820440223681818 2314 40-41 -18.026206635476843 2314 42-43 -18.03887875141225 2314 44-45 -15.57186050491218 2314 46-47 -14.202038679204046 2314 48-49 -13.679024162371817 2314 50-51 -13.583931191561556 2314 52-53 -13.58666667758485 2314 54-55 -13.917719440616303 2314 56-57 -13.176520426067622 2314 58-59 -12.531650976770724 2314 60-61 -13.556676697099338 2314 62-63 -16.38490110923078 2314 64-65 -14.412961789186902 2314 66-67 -14.254858213182029 2314 68-69 -16.696962102950955 2314 70-71 -16.34136082297237 2314 72-73 -15.143943747593969 2314 74-75 -13.978193769407662 2314 76-77 -14.632976501119785 2314 78-79 -14.155809471690159 2314 80-81 -14.194163829080567 2314 82-83 -14.740880588044655 2314 84-85 -15.341745800642256 2314 86-87 -15.583765171020797 2314 88-89 -14.941361735870053 2314 90-91 -14.633866147777548 2314 92-93 -14.730662808975808 2314 94-95 -14.644234816125635 2314 96-97 -14.386004717230177 2314 98-99 -14.41113675411004 2314 100-101 -14.085833681051788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76159.0 3 12647.0 4 8620.0 5 7218.0 6 7537.0 7 8635.0 8 10300.0 9 11677.0 10 12795.0 11 13914.0 12 15615.0 13 16588.0 14 18254.0 15 20309.0 16 21843.0 17 23296.0 18 21476.0 19 19563.0 20 19561.0 21 21046.0 22 24028.0 23 28702.0 24 35088.0 25 42690.0 26 50709.0 27 57732.0 28 64746.0 29 72614.0 30 81713.0 31 91739.0 32 105507.0 33 126561.0 34 153083.0 35 186531.0 36 228994.0 37 277548.0 38 251491.0 39 63823.0 40 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44461023331054 19.784221412714544 14.351912992309583 23.419255361665332 2 14.886843765637577 20.33261536453234 41.09218284272132 23.688358027108762 3 18.11839436018648 25.262559236783094 30.591088848790548 26.027957554239876 4 11.613640022101082 17.251982788999925 39.54510056427369 31.589276624625306 5 12.539145244419458 37.51570906955024 35.70749050948791 14.237655176542397 6 30.175700358020457 37.675457749773855 18.34425936901061 13.80458252319508 7 26.271946148556815 32.59756696226439 22.769921216787502 18.3605656723913 8 24.65999982668527 35.670251393017146 21.079577458686103 18.590171321611482 9 25.158267954330093 16.24937769529969 21.497144872534232 37.095209477835986 10-11 23.68921946814962 26.720936553258944 29.29570376522534 20.294140213366095 12-13 24.633737248521697 24.349679486206348 29.037500048795994 21.97908321647596 14-15 21.88670201481866 25.918831287730043 27.056634690955754 25.137832006495543 16-17 21.21004053228662 28.91035164743259 27.725821733773053 22.153786086507736 18-19 21.020891566844757 27.938344595875957 29.82019593984959 21.220567897429696 20-21 21.953260724272244 27.15218244223559 29.857701520908016 21.03685531258415 22-23 21.62229902417615 27.04820976205114 29.5641525219217 21.765338691851007 24-25 21.45285568812246 27.173134872162674 29.684305244740926 21.68970419497393 26-27 21.377929973233346 27.36405899500428 29.630836918475495 21.62717411328688 28-29 21.254571519129808 27.499546666487575 29.692792614595888 21.553089199786733 30-31 21.564419178551763 27.261855828257413 29.62525348893294 21.548471504257876 32-33 21.448278491498606 27.366868391727888 29.5218518152656 21.66300130150791 34-35 21.665256860964117 27.45415097296099 29.490469561742227 21.390122604332664 36-37 21.62008245905327 27.20128096277641 29.414794695333608 21.763841882836722 38-39 21.505642432024935 27.427216548995915 29.443900067044375 21.623240951934775 40-41 21.582296621796843 27.32566011893504 29.462365870942765 21.62967738832535 42-43 21.57529876628583 27.37715881564999 29.321373799556703 21.72616861850747 44-45 21.57864605975303 27.556830058160553 29.15528679232777 21.70923708975864 46-47 21.656094001198277 27.546743051521222 28.928597576323124 21.86856537095738 48-49 21.722982823330327 27.579782244868163 28.97468534855681 21.7225495832447 50-51 21.508490361706738 27.787329434697856 28.77132337015378 21.932856833441626 52-53 21.52544638089827 27.956372496035524 28.685237648509332 21.832943474556867 54-55 21.539655068745958 27.767263331067888 28.67193023317905 22.021151367007104 56-57 21.606936757537415 27.906627853153616 28.498991306039397 21.987444083269576 58-59 21.624393346313784 27.932202006042058 28.598030958093208 21.845373689550954 60-61 21.70862567065621 27.952794819950267 28.51587291953513 21.822706589858395 62-63 21.611867391033364 27.881712164651457 28.52302080303597 21.983399641279206 64-65 21.52562810764394 27.809799206382184 28.602118564839824 22.062454121134056 66-67 21.57157189651113 27.867645531225328 28.377030789047243 22.183751783216298 68-69 21.63818281602748 27.789413408401547 28.42418647248653 22.148217303084444 70-71 21.688779414412544 27.566662857855157 28.449333284857552 22.295224442874755 72-73 21.682719941552055 27.764980748189082 28.41734792454398 22.134951385714878 74-75 21.605634790376865 27.887057485353388 28.339635185108243 22.167672539161508 76-77 21.62900704283874 27.89978237836041 28.216086861677812 22.25512371712303 78-79 21.66049466073877 27.887122408276895 28.216454758244346 22.235928172739992 80-81 21.683693785404635 27.918069001814377 28.168519999722996 22.229717213057988 82-83 21.808800258999185 27.79967123031537 28.317929287682997 22.073599223002454 84-85 21.87367990055539 27.844857585075 27.99528399883658 22.286178515533024 86-87 21.673392681541806 27.92990661486683 28.127640198889214 22.26906050470215 88-89 21.804234013379318 27.770152941095 28.0951138142131 22.330499231312587 90-91 21.793413526128447 27.716245273611168 28.216433117269844 22.27390808299054 92-93 21.84452950790155 27.690817128571627 28.226149914821125 22.238503448705696 94-95 21.700487181617984 27.764980748189082 28.154691417016387 22.37984065317655 96-97 21.680837176770403 27.728429142255646 28.19624208805972 22.394491592914225 98-99 21.715051557457656 27.642752524203267 28.047265619589755 22.594930298749325 100-101 21.68774064763646 27.595380431018963 28.086717116106424 22.630161805238156 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 508.0 1 342.0 2 219.0 3 267.5 4 256.0 5 250.0 6 377.5 7 722.5 8 1227.5 9 1605.5 10 1518.0 11 1369.5 12 1517.5 13 1716.5 14 1892.0 15 2059.5 16 2262.0 17 2519.0 18 2847.5 19 3252.5 20 3793.5 21 4551.5 22 5578.0 23 7138.0 24 9136.0 25 11541.0 26 14894.5 27 18859.5 28 23252.0 29 28509.5 30 33828.0 31 39053.5 32 45235.0 33 51213.0 34 56656.0 35 61532.5 36 67149.5 37 72917.0 38 76097.5 39 77313.5 40 78591.0 41 79756.5 42 80945.5 43 82884.0 44 84723.5 45 86030.0 46 86057.0 47 85567.0 48 85597.5 49 84473.0 50 80857.5 51 77338.0 52 74552.0 53 70773.5 54 66212.5 55 60555.0 56 53772.0 57 47056.5 58 41084.0 59 35482.5 60 29326.5 61 23527.5 62 18759.0 63 14718.5 64 11353.0 65 8608.5 66 6417.5 67 4890.5 68 3728.0 69 2801.5 70 2190.0 71 1678.0 72 1272.0 73 977.5 74 776.5 75 606.0 76 444.0 77 325.5 78 233.0 79 177.5 80 136.0 81 97.0 82 72.0 83 60.5 84 48.0 85 36.5 86 29.0 87 23.0 88 17.5 89 18.5 90 17.0 91 10.5 92 5.5 93 4.0 94 5.0 95 3.5 96 2.5 97 1.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04046862231825044 2 0.5315888976606972 3 4.328194900347641E-5 4 0.5151417570393761 5 0.226581003033199 6 0.09452777662359248 7 0.10617062090552763 8 0.1078586169166632 9 0.10729595157961802 10-11 0.1517032312571848 12-13 0.21273077935208656 14-15 0.12999733383194137 16-17 0.119696229969114 18-19 0.1492578011384884 20-21 0.10826979543219623 22-23 0.1008902231271035 24-25 0.09545833852716722 26-27 0.14962569770501793 28-29 0.10943840805529008 30-31 0.12489006384953118 32-33 0.1012797606681348 34-35 0.1461415008102381 36-37 0.13439045165579425 38-39 0.1300622567554466 40-41 0.10965481780030746 42-43 0.10744743840113019 44-45 0.09004809490173266 46-47 0.09180101383637346 48-49 0.09708141161479758 50-51 0.08362072547471641 52-53 0.09164952701486129 54-55 0.3024326186617914 56-57 0.3226236478719131 58-59 0.33493736236340216 60-61 0.16170136147698785 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2310432.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.56955089190365 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2116184858451 64.28761672153605 2 9.06945560341713 13.526104623617485 3 2.2631331924396614 5.06282477306357 4 0.8428856020892439 2.514144032041869 5 0.43877884865867617 1.635977084267202 6 0.25508577158267154 1.141297885550072 7 0.18688262651936072 0.9755027470333663 8 0.11730137417797766 0.699768863316395 9 0.096369221473535 0.6467588608575511 >10 0.4851962710199148 6.468451764194792 >50 0.023353272436783968 1.193533217725457 >100 0.009644581302544862 1.1916631169641791 >500 5.902980750036968E-5 0.030944838428802787 >1k 1.7708942250110863E-4 0.36711230796047606 >5k 5.9029807500369536E-5 0.25829916344269466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5868 0.25397847675239954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4630 0.20039542388609577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2671 0.11560608578828548 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.32819490034764E-5 0.0 0.0 0.0 4 0.0 4.32819490034764E-5 0.0 4.32819490034764E-5 0.0 5 0.0 4.32819490034764E-5 0.0 4.32819490034764E-5 0.0 6 0.0 4.32819490034764E-5 0.0 4.32819490034764E-5 0.0 7 0.0 4.32819490034764E-5 0.0 4.32819490034764E-5 0.0 8 0.0 4.32819490034764E-5 0.0 4.32819490034764E-5 0.0 9 0.0 4.32819490034764E-5 0.0 4.32819490034764E-5 0.0 10-11 0.0 4.32819490034764E-5 0.0 1.0820487250869101E-4 4.32819490034764E-5 12-13 0.0 4.32819490034764E-5 0.0 1.2984584701042923E-4 8.65638980069528E-5 14-15 0.0 4.32819490034764E-5 0.0 1.5148682151216742E-4 1.0820487250869101E-4 16-17 0.0 4.32819490034764E-5 0.0 1.731277960139056E-4 1.2984584701042923E-4 18-19 0.0 4.32819490034764E-5 0.0 1.731277960139056E-4 1.2984584701042923E-4 20-21 0.0 4.32819490034764E-5 0.0 2.1640974501738202E-4 1.731277960139056E-4 22-23 0.0 4.32819490034764E-5 0.0 3.24614617526073E-4 1.731277960139056E-4 24-25 0.0 4.32819490034764E-5 0.0 6.059472860486697E-4 1.731277960139056E-4 26-27 0.0 4.32819490034764E-5 0.0 0.001060407750585172 1.731277960139056E-4 28-29 0.0 4.32819490034764E-5 0.0 0.0027267627872190137 1.731277960139056E-4 30-31 0.0 4.32819490034764E-5 0.0 0.0072497264580822975 2.1640974501738202E-4 32-33 0.0 4.32819490034764E-5 0.0 0.021035027215689533 2.1640974501738202E-4 34-35 0.0 4.32819490034764E-5 0.0 0.04358492264650074 2.1640974501738202E-4 36-37 0.0 4.32819490034764E-5 0.0 0.07937909447237573 2.1640974501738202E-4 38-39 0.0 4.32819490034764E-5 0.0 0.12917497680087534 2.1640974501738202E-4 40-41 0.0 4.32819490034764E-5 0.0 0.18005290785446185 2.5969169402085846E-4 42-43 4.32819490034764E-5 4.32819490034764E-5 0.0 0.22943761166742843 2.5969169402085846E-4 44-45 8.65638980069528E-5 4.32819490034764E-5 0.0 0.2924344884419883 2.5969169402085846E-4 46-47 8.65638980069528E-5 4.32819490034764E-5 0.0 0.370493483469758 3.0297364302433484E-4 48-49 8.65638980069528E-5 4.32819490034764E-5 0.0 0.44974273209512333 3.0297364302433484E-4 50-51 8.65638980069528E-5 4.32819490034764E-5 0.0 0.5309396684256451 3.0297364302433484E-4 52-53 8.65638980069528E-5 4.32819490034764E-5 0.0 0.6142141383083337 3.0297364302433484E-4 54-55 8.65638980069528E-5 4.32819490034764E-5 0.0 0.7033533122809934 3.24614617526073E-4 56-57 8.65638980069528E-5 4.32819490034764E-5 0.0 0.8101731624215731 3.895375410312876E-4 58-59 8.65638980069528E-5 4.32819490034764E-5 0.0 0.9269911427819559 4.3281949003476405E-4 60-61 1.2984584701042923E-4 4.32819490034764E-5 0.0 1.048483573634714 4.3281949003476405E-4 62-63 1.2984584701042923E-4 4.32819490034764E-5 0.0 1.172919177019709 4.761014390382405E-4 64-65 1.2984584701042923E-4 4.32819490034764E-5 0.0 1.307547679395022 4.761014390382405E-4 66-67 1.2984584701042923E-4 4.32819490034764E-5 0.0 1.4595106023462279 4.761014390382405E-4 68-69 1.2984584701042923E-4 4.32819490034764E-5 0.0 1.6353002382238473 4.761014390382405E-4 70-71 1.2984584701042923E-4 4.32819490034764E-5 0.0 1.8182313956870404 5.193833880417169E-4 72-73 1.2984584701042923E-4 4.32819490034764E-5 0.0 2.001855064334289 5.626653370451932E-4 74-75 1.2984584701042923E-4 4.32819490034764E-5 0.0 2.188270418692262 5.626653370451932E-4 76-77 1.2984584701042923E-4 4.32819490034764E-5 0.0 2.3877785626237866 5.843063115469314E-4 78-79 1.2984584701042923E-4 4.32819490034764E-5 0.0 2.6147058212490135 6.059472860486697E-4 80-81 1.2984584701042923E-4 4.32819490034764E-5 0.0 2.859421960914669 6.059472860486697E-4 82-83 1.2984584701042923E-4 4.32819490034764E-5 0.0 3.1136601293610893 6.059472860486697E-4 84-85 1.2984584701042923E-4 4.32819490034764E-5 0.0 3.3687639367875795 6.059472860486697E-4 86-87 1.2984584701042923E-4 4.32819490034764E-5 0.0 3.6408559091979336 6.059472860486697E-4 88-89 1.2984584701042923E-4 4.32819490034764E-5 0.0 3.942963913242199 6.059472860486697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3975 0.0 47.269974 1 GTATCAA 6835 0.0 38.71123 1 ATCAACG 7630 0.0 32.82144 3 CAACGCA 7760 0.0 32.675148 5 TCAACGC 7835 0.0 32.45891 4 AACGCAG 8030 0.0 31.678461 6 TATCAAC 8515 0.0 30.398703 2 ACGCAGA 8720 0.0 28.472214 7 CGCAGAG 8860 0.0 28.128458 8 GCAGAGT 10050 0.0 23.898148 9 GAGTACT 6275 0.0 23.266222 12-13 CAGAGTA 9795 0.0 21.41278 10-11 GTACATG 5760 0.0 21.137209 1 GTACTTT 7165 0.0 20.861774 14-15 GTGGTAT 2170 0.0 20.76369 1 GTATAGG 725 0.0 20.495575 1 AGAGTAC 8675 0.0 20.476704 10-11 TACATGG 5720 0.0 20.363676 2 GTATACG 375 7.2759576E-12 19.173279 1 ACATGGG 5795 0.0 18.685095 3 >>END_MODULE