FastQCFastQC Report
Sat 18 Jun 2016
SRR1931150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310432
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74370.32188785473885406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64110.2774805750612872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.17784552845528454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42800.049.227911
GTATCAA74850.038.1886331
ATCAACG89200.031.5699143
TCAACGC91800.030.6751164
CAACGCA91900.030.588745
TATCAAC94100.030.2792572
AACGCAG95100.029.5594656
ACGCAGA107900.025.7448147
CGCAGAG109050.024.950798
GTGGTAT22200.024.2090031
TGGTATC22750.022.5437372
GCAGAGT119800.022.1173329
GAGTACT74700.021.4222312-13
AGAGTAC105500.019.84913310-11
GTACTTT80000.019.82493814-15
CAGAGTA117950.019.76691610-11
TACATGG65000.019.5796532
GTACATG67600.019.559131
ACATGGG65050.019.126593
AGTACTT82850.017.42354212-13
Produced by FastQC (version 0.11.5)