##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931150_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310432 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26678127726763 31.0 31.0 34.0 30.0 34.0 2 31.26431853436933 31.0 31.0 34.0 28.0 34.0 3 31.423651074777357 31.0 31.0 34.0 28.0 34.0 4 35.081480433096495 35.0 35.0 37.0 32.0 37.0 5 34.95060014750488 35.0 35.0 37.0 32.0 37.0 6 35.071246416254624 36.0 35.0 37.0 32.0 37.0 7 34.92742439509148 36.0 35.0 37.0 32.0 37.0 8 34.981407373166576 36.0 35.0 37.0 32.0 37.0 9 36.38156933421975 38.0 35.0 39.0 32.0 39.0 10-11 36.254649346961955 38.0 35.0 39.0 32.0 39.0 12-13 36.19809130933089 38.0 35.0 39.0 32.0 39.0 14-15 37.137854089624796 39.0 36.0 40.0 31.5 41.0 16-17 37.18598902716029 39.0 36.0 40.0 31.5 41.0 18-19 37.252434393221705 39.0 36.0 40.0 31.5 41.0 20-21 37.23023940977272 39.0 36.0 40.0 31.0 41.0 22-23 37.119603173778756 39.0 36.0 40.0 31.0 41.0 24-25 36.9807027430368 39.0 36.0 40.0 31.0 41.0 26-27 36.735014274386785 39.0 35.5 40.0 30.0 41.0 28-29 36.44950013677096 38.0 35.0 40.0 30.0 41.0 30-31 36.232930897771496 38.0 35.0 40.0 30.0 41.0 32-33 36.06804117152117 38.0 35.0 40.0 29.5 41.0 34-35 35.820842812080166 38.0 34.0 40.0 29.0 41.0 36-37 36.06059018400022 38.0 35.0 40.0 30.0 41.0 38-39 36.03779899170372 38.0 35.0 40.0 30.0 41.0 40-41 35.88275525962244 38.0 35.0 40.0 29.0 41.0 42-43 35.72094742455091 38.0 34.5 40.0 28.5 41.0 44-45 35.48603616120275 38.0 34.0 40.0 27.5 41.0 46-47 35.26483142546502 38.0 34.0 40.0 27.0 41.0 48-49 34.991082403637066 38.0 34.0 40.0 26.0 41.0 50-51 34.62839958068447 38.0 33.0 40.0 24.0 41.0 52-53 34.50615447673855 38.0 33.0 40.0 24.0 41.0 54-55 34.2781142227947 38.0 33.0 40.0 24.0 41.0 56-57 34.07110315300342 37.5 33.0 40.0 24.0 41.0 58-59 33.82018903824047 37.0 32.5 40.0 23.0 41.0 60-61 33.53489260882813 37.0 32.0 40.0 22.5 41.0 62-63 33.26979196964031 36.5 31.5 39.5 22.0 41.0 64-65 33.02804800141272 36.0 31.0 39.0 22.0 41.0 66-67 32.791940208584364 36.0 31.0 39.0 21.5 40.5 68-69 32.47403104700766 35.5 31.0 39.0 20.5 40.0 70-71 31.63834533974599 35.0 30.0 38.0 16.0 40.0 72-73 31.60898286554203 35.0 30.0 38.0 18.0 40.0 74-75 31.298656052201494 35.0 30.0 37.0 18.0 39.0 76-77 30.570227775584826 34.0 29.5 36.0 17.0 39.0 78-79 30.589257117283694 34.0 30.0 36.0 14.5 39.0 80-81 30.304414499106663 34.0 29.5 36.0 12.0 38.5 82-83 29.944410612387642 34.0 29.0 35.5 9.0 37.0 84-85 29.576842555851027 34.0 29.0 35.0 7.0 37.0 86-87 29.23958181846512 34.0 29.0 35.0 4.0 36.0 88-89 28.914966335300065 34.0 29.0 35.0 2.0 36.0 90-91 28.59917084770294 34.0 29.0 35.0 2.0 36.0 92-93 28.25364931753023 33.5 28.5 35.0 2.0 35.0 94-95 27.860819318638246 33.0 27.0 35.0 2.0 35.0 96-97 27.488774393706457 33.0 27.0 35.0 2.0 35.0 98-99 27.017762046232047 33.0 26.0 35.0 2.0 35.0 100-101 26.341480078184514 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7913433546992508 1101 2 0.684223075259812 1101 3 0.5342109795896057 1101 4 0.8035394643375469 1101 5 0.792689922878516 1101 6 0.8413527579063782 1101 7 0.8031547651937103 1101 8 0.8053555538498856 1101 9 1.0186479946204372 1101 10-11 0.8730031832520098 1101 12-13 1.0243539788233775 1101 14-15 1.4410491369311558 1101 16-17 1.193130197078844 1101 18-19 0.9894991632572214 1101 20-21 0.947533287647758 1101 22-23 0.8590018671435473 1101 24-25 0.6889412514597524 1101 26-27 0.9535877607988965 1101 28-29 0.8831426526608084 1101 30-31 1.1594828087835793 1101 32-33 1.245124882572533 1101 34-35 0.8206624690360158 1101 36-37 1.190144795817531 1101 38-39 0.923991458220847 1101 40-41 0.9504811151358794 1101 42-43 1.0005013760789438 1101 44-45 0.8625267515102593 1101 46-47 0.9129224400758744 1101 48-49 0.8470109427643919 1101 50-51 0.9388370986394534 1101 52-53 0.8930776722367142 1101 54-55 0.9518850748920258 1101 56-57 0.6559090059006394 1101 58-59 0.7249638777858181 1101 60-61 0.9316498960714341 1101 62-63 0.8176254127908891 1101 64-65 0.754398945623052 1101 66-67 0.5599324913535142 1101 68-69 0.48286075792121963 1101 70-71 0.7841909387370762 1101 72-73 0.36772955513505323 1101 74-75 0.13766077221204753 1101 76-77 0.19597606457270444 1101 78-79 0.41555125902142365 1101 80-81 0.22726153291558404 1101 82-83 0.2048132545634047 1101 84-85 0.18390308434285885 1101 86-87 0.09805398714464175 1101 88-89 0.013401229537858939 1101 90-91 -0.01751393587493766 1101 92-93 -0.06878777691071747 1101 94-95 -0.047043721801117044 1101 96-97 -0.0698845636022547 1101 98-99 -0.12380918171639266 1101 100-101 -0.17074584364825185 1102 1 0.9612106807341227 1102 2 0.8823351633784959 1102 3 0.7008413345444993 1102 4 0.903600148501134 1102 5 0.955538631076827 1102 6 0.919446052688329 1102 7 0.8711867830938047 1102 8 1.0478349777277316 1102 9 1.4247434769032523 1102 10-11 1.1789739631673797 1102 12-13 1.2555701059003255 1102 14-15 1.6872049190368017 1102 16-17 1.5634784873644634 1102 18-19 1.2443076770532002 1102 20-21 1.2752528341997618 1102 22-23 0.923197423423531 1102 24-25 0.6501728459367371 1102 26-27 0.9755211730687208 1102 28-29 1.1857290633441835 1102 30-31 1.5266616770343404 1102 32-33 1.5106239444106393 1102 34-35 1.3777344083638994 1102 36-37 1.6942279822061295 1102 38-39 1.5244073392270892 1102 40-41 1.5843326158244508 1102 42-43 1.708271704231862 1102 44-45 1.630831414026666 1102 46-47 1.6170866505890302 1102 48-49 1.3730976662455774 1102 50-51 1.4203455909493954 1102 52-53 1.4413377894282249 1102 54-55 1.2125795300125972 1102 56-57 1.244784071771086 1102 58-59 1.385494241399961 1102 60-61 1.6344770175166303 1102 62-63 1.5628492843340211 1102 64-65 1.6010383314280006 1102 66-67 1.2678419693817204 1102 68-69 1.1998732946072401 1102 70-71 1.491187867947069 1102 72-73 1.0368325408607255 1102 74-75 1.0213114201272084 1102 76-77 1.1188516963038744 1102 78-79 1.0488703262125227 1102 80-81 0.804857413199457 1102 82-83 0.9059791151949668 1102 84-85 0.9012929290414107 1102 86-87 0.9443498394596226 1102 88-89 0.8441723490622763 1102 90-91 0.8261138449230288 1102 92-93 0.7375972690851462 1102 94-95 0.7101695264246644 1102 96-97 0.6721654000011412 1102 98-99 0.6718711639309696 1102 100-101 0.6584269334961625 1103 1 0.6276760610152934 1103 2 0.4250198080368257 1103 3 0.375190136524882 1103 4 0.6884373951804861 1103 5 0.6565914012085443 1103 6 0.589383334467712 1103 7 0.5656890091749744 1103 8 0.5494782135814873 1103 9 0.9125383633823461 1103 10-11 0.7195690011308358 1103 12-13 0.8252414818216991 1103 14-15 1.2699652596918867 1103 16-17 1.0603459246924274 1103 18-19 0.8731336963397425 1103 20-21 0.8310570406618609 1103 22-23 0.6682335395122863 1103 24-25 0.5031586581330743 1103 26-27 0.7624507974983459 1103 28-29 0.628596251277159 1103 30-31 1.1323729376798184 1103 32-33 1.2174118755406766 1103 34-35 0.9341498634635599 1103 36-37 1.2408277817664555 1103 38-39 1.0747009556391305 1103 40-41 0.998256360998198 1103 42-43 1.1273578101600705 1103 44-45 1.149398524370092 1103 46-47 1.171643766659308 1103 48-49 1.1262010173752444 1103 50-51 1.0208793654963273 1103 52-53 0.5324519384371129 1103 54-55 0.7028085577416263 1103 56-57 0.781848900612232 1103 58-59 0.9327235323792493 1103 60-61 1.0823420347895336 1103 62-63 0.8638140734567088 1103 64-65 0.838827560335659 1103 66-67 0.8013310976077008 1103 68-69 0.5969153084255687 1103 70-71 0.9795091394920306 1103 72-73 0.6077928039262552 1103 74-75 0.37088248384300826 1103 76-77 0.6513553679522843 1103 78-79 0.6639511024775082 1103 80-81 0.48051865629491175 1103 82-83 0.5304214402951857 1103 84-85 0.5602475945750243 1103 86-87 0.5428956126764497 1103 88-89 0.37518715573498085 1103 90-91 0.3403766980016698 1103 92-93 -0.03596247486933635 1103 94-95 0.006642319279038134 1103 96-97 0.003655096293467608 1103 98-99 -0.18138136234786728 1103 100-101 -0.1476789716229021 1104 1 0.5088890279490741 1104 2 0.3927747987301444 1104 3 0.2340795318488631 1104 4 0.6122340941716828 1104 5 0.41847510026036616 1104 6 0.5399024429124353 1104 7 0.4446282945925617 1104 8 0.39669642548641093 1104 9 0.7295165998955184 1104 10-11 0.47524916341958345 1104 12-13 0.6162230952340977 1104 14-15 1.010720941931055 1104 16-17 0.8247466373439067 1104 18-19 0.6137491609215928 1104 20-21 0.5692649489826422 1104 22-23 0.32784322025609924 1104 24-25 0.3593451307777187 1104 26-27 0.2061456057403106 1104 28-29 0.5106144312991177 1104 30-31 0.7907657896104183 1104 32-33 0.9414016123715641 1104 34-35 0.7625717243954924 1104 36-37 0.9458826900580419 1104 38-39 0.3984872140841844 1104 40-41 0.6737256242548852 1104 42-43 0.7901257561117205 1104 44-45 0.780011777352378 1104 46-47 0.7892785788080587 1104 48-49 0.5743444525170034 1104 50-51 0.5345478366186711 1104 52-53 0.42477112052697663 1104 54-55 0.6521003968269881 1104 56-57 0.6485580966003681 1104 58-59 0.6207018609488486 1104 60-61 0.9348507408578541 1104 62-63 0.11086972718084098 1104 64-65 -0.7809604741248819 1104 66-67 -0.10565783765867565 1104 68-69 0.385447378864459 1104 70-71 0.8967394085437732 1104 72-73 0.3194101006555563 1104 74-75 0.19959516450374792 1104 76-77 -0.030770866109044448 1104 78-79 -0.13196260247939406 1104 80-81 0.051664803733388 1104 82-83 -0.18882553812095182 1104 84-85 -0.2888121388205782 1104 86-87 -0.1827311007518908 1104 88-89 -0.06968692903327423 1104 90-91 0.06983099423027284 1104 92-93 0.0703596126112167 1104 94-95 0.0367392142830667 1104 96-97 0.005609386908872693 1104 98-99 -0.04212771545387639 1104 100-101 -0.28814664073168217 1105 1 -0.2717400494992148 1105 2 -1.6522984609290425 1105 3 -0.25104300320940354 1105 4 0.42326189098896805 1105 5 0.5032241271318867 1105 6 0.06530386702440438 1105 7 -0.18324497785970095 1105 8 -0.1630513744629738 1105 9 0.4295487480128699 1105 10-11 0.841849571695164 1105 12-13 0.9483212809884591 1105 14-15 1.2115040629011702 1105 16-17 0.8366182808846929 1105 18-19 0.6132801044495437 1105 20-21 0.9402582899660885 1105 22-23 0.4153495134017078 1105 24-25 -0.03863863635301357 1105 26-27 0.8731521114605343 1105 28-29 0.4295860802990248 1105 30-31 0.8937455699334578 1105 32-33 1.1057536237794565 1105 34-35 0.7159289693845423 1105 36-37 0.6232453147560761 1105 38-39 0.6645838122840004 1105 40-41 1.0213762199876228 1105 42-43 1.2888701598303172 1105 44-45 1.280314165411589 1105 46-47 1.3926605552390185 1105 48-49 1.1138333553508275 1105 50-51 0.6705816577615451 1105 52-53 0.7432515043854195 1105 54-55 -0.12824848744585182 1105 56-57 0.2570333163572869 1105 58-59 -0.49575366022838097 1105 60-61 0.3374892300397079 1105 62-63 0.8339169358364558 1105 64-65 1.2728162817385638 1105 66-67 0.8337728070516874 1105 68-69 0.7255101504071355 1105 70-71 0.21930709742499488 1105 72-73 -0.10249933760158214 1105 74-75 -0.18376768848451164 1105 76-77 0.14125705974184655 1105 78-79 -0.21217760059627366 1105 80-81 -0.10964967929676561 1105 82-83 -0.30766659573335176 1105 84-85 -0.2263720029713454 1105 86-87 -0.3618078321871785 1105 88-89 -0.7471173101621638 1105 90-91 -0.8451867258340684 1105 92-93 -0.5559544347926462 1105 94-95 -0.8529232635702968 1105 96-97 -0.771886765518417 1105 98-99 -1.4598960846841003 1105 100-101 -1.0498836674774807 1106 1 0.3406646007808263 1106 2 0.352506726977893 1106 3 -0.26662169606285957 1106 4 0.3795105921950892 1106 5 0.10343999482870458 1106 6 0.41457808003684704 1106 7 0.3647400395336575 1106 8 0.5180407958716913 1106 9 0.9233356597737838 1106 10-11 0.1948373256802256 1106 12-13 0.29072582565655836 1106 14-15 0.25143085053078096 1106 16-17 -0.2368491889297033 1106 18-19 0.38093581915813957 1106 20-21 0.5550981108233941 1106 22-23 0.5023556572209458 1106 24-25 0.42558601825712117 1106 26-27 0.7786825039362384 1106 28-29 0.7556711342527507 1106 30-31 0.34495933843767546 1106 32-33 0.3609752799423944 1106 34-35 -0.9602747659854813 1106 36-37 -0.7256743993586241 1106 38-39 -0.6151612078801136 1106 40-41 0.4913240321586443 1106 42-43 0.6690828467440326 1106 44-45 0.6735745472981449 1106 46-47 0.1386407008157633 1106 48-49 0.09774745339536395 1106 50-51 0.7700903914029595 1106 52-53 0.052941392011213395 1106 54-55 0.7617997383822868 1106 56-57 0.40796962832004624 1106 58-59 0.7408592111334826 1106 60-61 0.8386921189611058 1106 62-63 0.7170563547782081 1106 64-65 0.8160411904385967 1106 66-67 0.654991169675256 1106 68-69 0.6531624389402033 1106 70-71 1.0049149780015014 1106 72-73 0.4170230292986403 1106 74-75 0.5198594293066598 1106 76-77 0.5563947217979326 1106 78-79 0.5956469161592146 1106 80-81 0.6616377271318328 1106 82-83 0.5287433842221212 1106 84-85 0.6641654934603665 1106 86-87 0.697528527238827 1106 88-89 0.710061979771492 1106 90-91 0.8144765491362449 1106 92-93 0.30146987483919574 1106 94-95 0.4727733170568058 1106 96-97 0.7484950185343635 1106 98-99 0.669117596100083 1106 100-101 0.5045013945835066 1107 1 0.3700388179501104 1107 2 0.4985434110119762 1107 3 0.1871458967836439 1107 4 -0.8323014740827261 1107 5 0.26521203946519023 1107 6 0.46283874444550577 1107 7 0.6253575092271433 1107 8 0.5988354107375073 1107 9 0.9530770507975745 1107 10-11 0.8390951156950734 1107 12-13 0.979277165763925 1107 14-15 1.3672775985253764 1107 16-17 1.1646415245672301 1107 18-19 1.0421984834136282 1107 20-21 0.26136118888287996 1107 22-23 0.6135149939981162 1107 24-25 0.6156282880276862 1107 26-27 0.9956779286226762 1107 28-29 0.9212634545444587 1107 30-31 1.1844805857294176 1107 32-33 1.3457667474272768 1107 34-35 1.048392883928912 1107 36-37 1.1861740754956855 1107 38-39 1.1066562687306956 1107 40-41 0.6539806600636169 1107 42-43 0.4067924045048983 1107 44-45 -0.02893603867303085 1107 46-47 0.47033580261154384 1107 48-49 0.8396747239441282 1107 50-51 -0.8395230477159714 1107 52-53 0.6732859425212396 1107 54-55 1.2113684557875004 1107 56-57 0.9158939750655719 1107 58-59 0.9633168487202326 1107 60-61 1.1127630054404705 1107 62-63 1.1820111698510516 1107 64-65 1.225547812537954 1107 66-67 1.004692304362905 1107 68-69 1.1174514618933564 1107 70-71 1.4130653111446065 1107 72-73 0.9497186412468288 1107 74-75 0.8820081899945365 1107 76-77 1.0346985762278607 1107 78-79 1.0639347063469948 1107 80-81 1.0017674824810214 1107 82-83 1.050728420575112 1107 84-85 1.0401334551482933 1107 86-87 1.1747061553883675 1107 88-89 1.2384954104414128 1107 90-91 1.4984149009857646 1107 92-93 1.3477787122105127 1107 94-95 1.2675218971532054 1107 96-97 1.3101019430194576 1107 98-99 1.2338584683248222 1107 100-101 1.202708573280141 1108 1 0.5785951296309619 1108 2 0.4762576363873414 1108 3 0.29295769877029443 1108 4 0.660260911614067 1108 5 0.5314551544727024 1108 6 0.5340342299915761 1108 7 0.5327882626581157 1108 8 0.5434046551625116 1108 9 0.6751211404777493 1108 10-11 0.5895055620162566 1108 12-13 0.8802812115345162 1108 14-15 1.2185144583470304 1108 16-17 0.9843558925200924 1108 18-19 0.7378838255889093 1108 20-21 0.5243558416905856 1108 22-23 0.609185274414827 1108 24-25 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1.5355031155815055 2311 82-83 1.551551095024628 2311 84-85 1.7494761927536828 2311 86-87 1.6386473640685786 2311 88-89 1.7516974082511574 2311 90-91 1.7873184978938923 2311 92-93 2.0197302980185157 2311 94-95 2.0284372852854275 2311 96-97 1.9755585206937454 2311 98-99 2.3155477817970116 2311 100-101 2.4688510674057937 2312 1 -0.26882903571863537 2312 2 -0.14997499121426827 2312 3 -0.3630769739390267 2312 4 0.1951879672628678 2312 5 -1.4395704460974699 2312 6 -0.1165143147937755 2312 7 0.29146392076224714 2312 8 -2.4823815742279542 2312 9 -5.330679860041823 2312 10-11 -0.8158793034250209 2312 12-13 -5.986220968771185 2312 14-15 -1.9715159135970737 2312 16-17 -2.64878659038974 2312 18-19 -2.427500795027015 2312 20-21 -3.9794484721319776 2312 22-23 -2.132962632736522 2312 24-25 -2.924254489360166 2312 26-27 -3.199703352699281 2312 28-29 -6.110794220950844 2312 30-31 -6.733177301554633 2312 32-33 -1.7418454575152822 2312 34-35 0.6163268969563873 2312 36-37 0.8948375591744053 2312 38-39 0.8383633230200331 2312 40-41 -2.3954195283298674 2312 42-43 -4.894844186927507 2312 44-45 -3.472543435272101 2312 46-47 0.005188597114894833 2312 48-49 -1.5241994402906585 2312 50-51 -7.436978966103425 2312 52-53 -1.6496418830731798 2312 54-55 0.6150615221684959 2312 56-57 1.0110927117210267 2312 58-59 1.1602927023397243 2312 60-61 1.4970341504541977 2312 62-63 1.4533085606628617 2312 64-65 1.4919168051869178 2312 66-67 1.1367585991679192 2312 68-69 1.2840642578742951 2312 70-71 1.5214115734425135 2312 72-73 1.1436415661480162 2312 74-75 0.9773262117398254 2312 76-77 1.13190976778586 2312 78-79 1.0342704284604487 2312 80-81 1.0306798795110552 2312 82-83 0.1760424097147819 2312 84-85 0.5431371306682209 2312 86-87 1.0444922425911578 2312 88-89 1.23414804610319 2312 90-91 1.3593854352598314 2312 92-93 1.5416847063247587 2312 94-95 1.31264398351869 2312 96-97 1.5426763153490093 2312 98-99 1.5002443917607202 2312 100-101 1.427175407407681 2313 1 -0.29919794217450146 2313 2 -0.17261442907109625 2313 3 -0.45532554662237956 2313 4 -1.4402973203963327 2313 5 -2.9277786147404186 2313 6 -1.8100584518161753 2313 7 -2.7227214108143905 2313 8 -3.8947004148076623 2313 9 -2.597961370801599 2313 10-11 -0.7824910732976562 2313 12-13 -4.04092013433992 2313 14-15 -2.0672888604569692 2313 16-17 -4.661357956221977 2313 18-19 -2.7920045280090022 2313 20-21 -3.5397031932562655 2313 22-23 -3.33094243071632 2313 24-25 -0.9392522934884084 2313 26-27 -2.975427990380446 2313 28-29 -4.45913633777122 2313 30-31 -2.607419725797058 2313 32-33 -5.465110718824022 2313 34-35 -2.3940266383971505 2313 36-37 -2.920017513289366 2313 38-39 -1.8942783107085575 2313 40-41 -1.1545214167795876 2313 42-43 -2.861730440770284 2313 44-45 -5.33276521831997 2313 46-47 -2.348225983473604 2313 48-49 -0.9026798970319874 2313 50-51 0.829544968898773 2313 52-53 -0.07096379787335394 2313 54-55 -3.5274944725614077 2313 56-57 -6.523799690562676 2313 58-59 -0.5389936393379244 2313 60-61 -5.857862643573036 2313 62-63 -0.9806835341987963 2313 64-65 -2.8834345342948637 2313 66-67 -3.5575870310472766 2313 68-69 -4.19358176759782 2313 70-71 -3.3453978248886216 2313 72-73 -0.17944802541983762 2313 74-75 1.7381738182396056 2313 76-77 -1.509888651186472 2313 78-79 -0.10008398524179185 2313 80-81 1.281635083727128 2313 82-83 1.9440046407204932 2313 84-85 1.7826957604442413 2313 86-87 1.811609062968845 2313 88-89 -2.1518862094962827 2313 90-91 1.1183006746098556 2313 92-93 1.749271443259321 2313 94-95 2.4339549189600618 2313 96-97 2.7534690250547627 2313 98-99 2.955327834887644 2313 100-101 2.952241283560511 2314 1 -28.765864608841166 2314 2 -14.741824603100547 2314 3 -4.817039067372047 2314 4 -3.8372183377992783 2314 5 -1.3020758034954412 2314 6 -1.1458014237037233 2314 7 -0.1506999918050198 2314 8 -4.313575916815697 2314 9 -3.123931919663711 2314 10-11 -0.6023036569656597 2314 12-13 0.4317036943080055 2314 14-15 -0.8032995699616592 2314 16-17 -1.3317060151242543 2314 18-19 -3.2312950233235966 2314 20-21 -2.6910418813419454 2314 22-23 -3.9245836623093595 2314 24-25 -0.5839644755499904 2314 26-27 -2.13245610283024 2314 28-29 -1.9855084930590436 2314 30-31 -4.691556271981799 2314 32-33 -2.1612285233755557 2314 34-35 -2.084521952988851 2314 36-37 -4.653551649835549 2314 38-39 -6.917571483399321 2314 40-41 -6.100840024543974 2314 42-43 -4.086496170355289 2314 44-45 -3.259003504799221 2314 46-47 -6.201505769283507 2314 48-49 -4.230256871596914 2314 50-51 -1.2350467312351014 2314 52-53 -3.2030253775253 2314 54-55 -4.2264904564971495 2314 56-57 -1.2393954067607993 2314 58-59 -0.5511757009175753 2314 60-61 0.9575322694122406 2314 62-63 2.158473092307233 2314 64-65 0.1343700172446347 2314 66-67 1.2404718712284932 2314 68-69 2.17736870094301 2314 70-71 2.404066699877106 2314 72-73 1.7456523761865874 2314 74-75 1.9256570846385337 2314 76-77 0.17987038495810737 2314 78-79 -2.1147426197799426 2314 80-81 -1.393465317879297 2314 82-83 -0.1070663097480491 2314 84-85 0.8692419451831981 2314 86-87 1.9831619411482286 2314 88-89 1.7828527463270127 2314 90-91 2.403708974475986 2314 92-93 2.6036221795377656 2314 94-95 2.485360541450021 2314 96-97 2.6298411803425807 2314 98-99 2.4689155189572567 2314 100-101 2.977743291592642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 32.0 5 94.0 6 216.0 7 569.0 8 1467.0 9 2663.0 10 4041.0 11 5925.0 12 7915.0 13 10086.0 14 12453.0 15 15014.0 16 17343.0 17 19377.0 18 17650.0 19 14864.0 20 14415.0 21 15112.0 22 16574.0 23 18765.0 24 22239.0 25 27806.0 26 34669.0 27 44057.0 28 55245.0 29 68233.0 30 84710.0 31 102349.0 32 123267.0 33 149574.0 34 182382.0 35 224679.0 36 279569.0 37 345535.0 38 304511.0 39 66972.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.4455539536162 21.16894950249813 15.272228064532628 23.113268479353046 2 15.11777883962826 21.58838693369898 40.276407182726 23.01742704394676 3 18.325161404255983 25.971844934183075 31.083888660929006 24.61910500063194 4 12.719786498062694 17.866244464219555 38.596032589689166 30.817936448028586 5 12.174400436282424 38.430681734568886 36.107131572910674 13.287786256238016 6 30.152369773271836 38.81685329843077 17.67232275176244 13.35845417653495 7 26.059152574064072 33.3304334427501 22.677966717912497 17.932447265273336 8 24.624312682649823 36.765591889309015 20.378569895153806 18.231525532887353 9 25.007357931330592 16.366809323970582 21.27177947673855 37.35405326796028 10-11 23.738396109472166 27.31722898574812 28.98070577277323 19.96366913200648 12-13 24.563631390146952 24.7671863963103 29.08176912369635 21.587413089846404 14-15 21.720093904516556 26.507423719893076 26.96225208099611 24.810230294594255 16-17 21.092635489813162 29.440424994113656 27.657165413221424 21.80977410285176 18-19 20.91671600808853 28.337427805709066 29.891379620780878 20.854476565421532 20-21 21.732039722441517 27.441707871082116 30.015122712981814 20.81112969349455 22-23 21.477866476918603 27.275699955679283 29.769952112851623 21.47648145455049 24-25 21.423092157809183 27.40305756913946 29.89184006095225 21.28201021209911 26-27 21.417548944621124 27.569708940257893 29.711914009660585 21.300828105460408 28-29 21.239997558770227 27.69324129954798 29.819859750483808 21.24690139119798 30-31 21.63771633755974 27.412921778709602 29.700843232799258 21.2485186509314 32-33 21.515156040114864 27.549243848130345 29.6079428137493 21.32765729800549 34-35 21.668914106673437 27.49773687011355 29.63523951304673 21.198109510166287 36-37 21.596038286636446 27.417958985281643 29.575147662897717 21.410855065184194 38-39 21.414520198249413 27.54166661256402 29.665022379018907 21.37879081016766 40-41 21.586117017984353 27.436107301039602 29.62027738090034 21.3574983000757 42-43 21.619789725724125 27.492922254958103 29.401695196222665 21.48559282309511 44-45 21.559683754947955 27.67107415588527 29.269893593101003 21.499348496065764 46-47 21.702494012780384 27.596303694205965 29.03678662897942 21.66441566403423 48-49 21.760995344441824 27.661080386461762 28.98670993874767 21.591214330348745 50-51 21.688454728086942 27.808504762472047 28.799943684241875 21.703096825199136 52-53 21.72918483635915 27.960024151714318 28.63107823553981 21.679712776386722 54-55 21.74902774783013 27.766491483755097 28.75041241157506 21.73406835683971 56-57 21.81185236033966 27.940117961798105 28.502897106096796 21.74513257176543 58-59 21.779035088535593 27.877506845680518 28.676135307393942 21.667322758389947 60-61 21.782415848698086 27.90030504069193 28.651858648969476 21.66542046164051 62-63 21.77467001681567 27.83105986335374 28.60109637305251 21.793173746778077 64-65 21.604748693128954 27.763064003080263 28.676061117824116 21.956126185966674 66-67 21.673857639514107 27.856680332977824 28.4461698547031 22.023292172804965 68-69 21.775695190475655 27.81220702795305 28.446584838645173 21.965512942926125 70-71 21.776649592390246 27.567049791845033 28.44330038808846 22.213000227676257 72-73 21.700866437477885 27.773070642734943 28.448604036882298 22.077458882904878 74-75 21.733979828233764 27.851298959166293 28.26698320534365 22.147738007256297 76-77 21.574493428068862 27.93674516280938 28.134630233653272 22.354131175468485 78-79 21.60582955914738 27.976889170510105 28.086782039029927 22.330499231312587 80-81 21.611079515206285 28.020828193588542 28.01022408854471 22.357868202660462 82-83 21.71543813382727 27.88771817746439 28.140096696767277 22.25674699194107 84-85 21.748859466294498 27.8827136835695 27.95246255948294 22.41596429065307 86-87 21.53311746855627 27.99795879236339 28.002741520180297 22.46618221890004 88-89 21.654692622680297 27.85071663554937 27.940010678061217 22.554580063709114 90-91 21.64319562777103 27.839350796539385 27.995427632568465 22.522025943121115 92-93 21.63989481272471 27.799964118076204 28.02399890406476 22.536142165134326 94-95 21.55883716969401 27.821328164275283 27.91756630682503 22.702268359205675 96-97 21.45845776958302 27.816349408332684 27.981817158784985 22.743375663299314 98-99 21.369557130128964 27.821306862263683 27.90419306815951 22.90494293944784 100-101 21.380156010183686 27.811377610954473 27.850960152564834 22.957506226297006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 529.0 1 346.5 2 212.0 3 291.5 4 260.0 5 235.5 6 418.0 7 852.5 8 1419.5 9 1785.0 10 1753.5 11 1619.0 12 1653.5 13 1810.5 14 2043.5 15 2292.5 16 2600.5 17 2818.0 18 3034.0 19 3436.0 20 4057.0 21 4855.0 22 5788.5 23 7270.0 24 9444.5 25 11994.0 26 15436.0 27 19779.5 28 24393.0 29 29583.5 30 34917.5 31 39916.5 32 45447.5 33 51654.5 34 57387.0 35 61979.5 36 67360.5 37 73337.0 38 76427.5 39 76932.0 40 78088.0 41 79101.5 42 80105.5 43 82203.0 44 83739.5 45 84978.5 46 85563.5 47 85330.0 48 85142.0 49 83868.0 50 80539.0 51 76969.0 52 73929.5 53 70374.0 54 66287.5 55 60776.0 56 53867.0 57 46942.0 58 40732.5 59 35141.5 60 28996.0 61 23126.0 62 18375.5 63 14288.5 64 11009.5 65 8388.0 66 6187.5 67 4655.0 68 3572.0 69 2677.0 70 2006.0 71 1540.5 72 1179.5 73 900.5 74 718.5 75 540.5 76 385.0 77 273.5 78 201.0 79 152.5 80 115.0 81 88.5 82 74.0 83 52.0 84 39.0 85 32.0 86 28.0 87 19.0 88 9.5 89 6.0 90 5.5 91 3.5 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1517465132061883 2 0.0 3 0.0036356837162920177 4 3.462555920278113E-4 5 8.656389800695282E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.018481392224484423 26-27 0.009478746831761333 28-29 0.0052371158294206455 30-31 0.0016447140621321035 32-33 7.141521585573607E-4 34-35 0.0011036896995886484 36-37 7.57434107560837E-4 38-39 3.8953754103128766E-4 40-41 0.001514868215121674 42-43 9.089209290730044E-4 44-45 3.6789656652954944E-4 46-47 0.031184644257004752 48-49 0.017204574728881872 50-51 0.08740789601252061 52-53 0.001601432113128627 54-55 0.03661652885694104 56-57 9.954848270799573E-4 58-59 0.0014499452916164595 60-61 0.003657324690793756 62-63 0.0037655295633024473 64-65 0.010322744837329123 66-67 0.0055400894724449805 68-69 0.002510353042201632 70-71 0.005799781166465838 72-73 0.0047393734158806666 74-75 0.001060407750585172 76-77 0.0 78-79 0.0 80-81 2.5969169402085846E-4 82-83 5.843063115469315E-4 84-85 2.1640974501738205E-5 86-87 0.001514868215121674 88-89 0.0037871705378041856 90-91 0.0013633813936095066 92-93 0.0033110690987659453 94-95 7.57434107560837E-4 96-97 5.193833880417169E-4 98-99 0.0015365091896234126 100-101 0.003029736430243348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2310432.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.84513759996744 #Duplication Level Percentage of deduplicated Percentage of total 1 83.42677963331144 58.269549030107996 2 10.731131118549635 14.990346591567839 3 2.730045323813808 5.720411738877694 4 1.0997933697358262 3.0726087696292264 5 0.5495204371569617 1.919066527361113 6 0.3379658886654086 1.4163164398758445 7 0.20887379958295593 1.0212173491029706 8 0.15050788033303233 0.8409794889392064 9 0.1147953362998072 0.7216106453725115 >10 0.5990138594304935 7.605303111973634 >50 0.03633970436487628 1.7163542997259313 >100 0.014735191920457235 1.7321618069855464 >500 1.869213131832345E-4 0.07559160462151553 >1k 1.8692131318203764E-4 0.2958386460645662 >5k 1.246142087880251E-4 0.6026439497943679 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7437 0.32188785473885406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6411 0.2774805750612872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4109 0.17784552845528454 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.32819490034764E-5 0.0 5 0.0 0.0 0.0 4.32819490034764E-5 0.0 6 0.0 0.0 0.0 1.2984584701042923E-4 4.32819490034764E-5 7 0.0 0.0 0.0 1.2984584701042923E-4 4.32819490034764E-5 8 0.0 0.0 0.0 1.2984584701042923E-4 4.32819490034764E-5 9 0.0 0.0 0.0 1.2984584701042923E-4 4.32819490034764E-5 10-11 0.0 0.0 0.0 1.2984584701042923E-4 4.32819490034764E-5 12-13 0.0 0.0 0.0 1.2984584701042923E-4 8.65638980069528E-5 14-15 0.0 0.0 0.0 1.5148682151216742E-4 8.65638980069528E-5 16-17 0.0 0.0 0.0 1.731277960139056E-4 8.65638980069528E-5 18-19 0.0 0.0 0.0 1.731277960139056E-4 1.2984584701042923E-4 20-21 0.0 0.0 0.0 2.3805071951912024E-4 1.731277960139056E-4 22-23 0.0 0.0 0.0 3.6789656652954944E-4 1.947687705156438E-4 24-25 0.0 0.0 0.0 6.925111840556225E-4 2.1640974501738202E-4 26-27 0.0 0.0 0.0 0.0012335355465990776 2.1640974501738202E-4 28-29 0.0 0.0 0.0 0.002986454481239872 2.1640974501738202E-4 30-31 0.0 4.32819490034764E-5 0.0 0.00783403276962923 2.5969169402085846E-4 32-33 0.0 4.32819490034764E-5 0.0 0.02287451004833728 3.0297364302433484E-4 34-35 0.0 6.49229235052146E-5 0.0 0.046787786872757996 3.0297364302433484E-4 36-37 0.0 8.65638980069528E-5 0.0 0.08504902979183114 3.24614617526073E-4 38-39 0.0 8.65638980069528E-5 0.0 0.13733362418803063 3.462555920278112E-4 40-41 0.0 8.65638980069528E-5 0.0 0.19139277849337266 3.462555920278112E-4 42-43 0.0 8.65638980069528E-5 0.0 0.24330947632304262 3.8953754103128766E-4 44-45 0.0 8.65638980069528E-5 0.0 0.30946593537485634 3.8953754103128766E-4 46-47 0.0 8.65638980069528E-5 0.0 0.39163671555795626 4.3281949003476405E-4 48-49 0.0 8.65638980069528E-5 0.0 0.4751275951856623 4.3281949003476405E-4 50-51 0.0 8.65638980069528E-5 0.0 0.560782572263542 4.3281949003476405E-4 52-53 0.0 8.65638980069528E-5 0.0 0.646177857647401 4.977424135399788E-4 54-55 0.0 1.2984584701042923E-4 0.0 0.7382385631777952 5.193833880417169E-4 56-57 0.0 1.2984584701042923E-4 0.0 0.848369482417141 5.410243625434551E-4 58-59 0.0 1.2984584701042923E-4 0.0 0.9673299192531959 5.626653370451932E-4 60-61 0.0 1.2984584701042923E-4 0.0 1.08652840680877 5.626653370451932E-4 62-63 0.0 1.2984584701042923E-4 0.0 1.2100767302391935 6.492292350521461E-4 64-65 0.0 1.2984584701042923E-4 0.0 1.3444022589714824 6.492292350521461E-4 66-67 0.0 1.2984584701042923E-4 0.0 1.4949801595545766 6.708702095538843E-4 68-69 0.0 1.2984584701042923E-4 0.0 1.6679131867979669 7.141521585573606E-4 70-71 0.0 1.5148682151216742E-4 0.0 1.8475332751623939 7.357931330590989E-4 72-73 0.0 1.731277960139056E-4 0.0 2.0369567249761085 7.790750820625753E-4 74-75 0.0 1.731277960139056E-4 0.0 2.226942840126868 8.223570310660518E-4 76-77 0.0 1.731277960139056E-4 0.0 2.4301732316726916 8.223570310660518E-4 78-79 0.0 1.731277960139056E-4 0.0 2.6611040705807403 8.223570310660518E-4 80-81 0.0 1.731277960139056E-4 0.0 2.9087850237531336 8.439980055677899E-4 82-83 0.0 1.731277960139056E-4 0.0 3.1669402085843688 8.656389800695281E-4 84-85 0.0 1.731277960139056E-4 0.0 3.423883498843506 8.656389800695281E-4 86-87 0.0 1.731277960139056E-4 0.0 3.6995462320466475 9.089209290730045E-4 88-89 0.0 1.731277960139056E-4 0.0 4.004489203750641 9.089209290730045E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4280 0.0 49.22791 1 GTATCAA 7485 0.0 38.188633 1 ATCAACG 8920 0.0 31.569914 3 TCAACGC 9180 0.0 30.675116 4 CAACGCA 9190 0.0 30.58874 5 TATCAAC 9410 0.0 30.279257 2 AACGCAG 9510 0.0 29.559465 6 ACGCAGA 10790 0.0 25.744814 7 CGCAGAG 10905 0.0 24.95079 8 GTGGTAT 2220 0.0 24.209003 1 TGGTATC 2275 0.0 22.543737 2 GCAGAGT 11980 0.0 22.117332 9 GAGTACT 7470 0.0 21.42223 12-13 AGAGTAC 10550 0.0 19.849133 10-11 GTACTTT 8000 0.0 19.824938 14-15 CAGAGTA 11795 0.0 19.766916 10-11 TACATGG 6500 0.0 19.579653 2 GTACATG 6760 0.0 19.55913 1 ACATGGG 6505 0.0 19.12659 3 AGTACTT 8285 0.0 17.423542 12-13 >>END_MODULE