FastQCFastQC Report
Sat 18 Jun 2016
SRR1931149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220095
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48450.21823390440499166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.16093905891414556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.048.576031
GTATCAA46050.043.7127381
ATCAACG54000.035.803623
TCAACGC55150.035.0633934
CAACGCA55200.034.929445
AACGCAG58550.033.478896
TATCAAC60850.032.8466452
ACGCAGA66700.028.8227087
CGCAGAG68050.027.8993828
GTACATG52100.023.9529151
GCAGAGT77350.023.1306159
TACATGG53300.021.9793152
ACATGGG50950.021.55013
GTGGTAT15450.021.0443741
GAGTACT50900.020.28670112-13
AGAGTAC70250.020.28526510-11
CAGAGTA73600.019.87913510-11
CATGGGG32050.019.3668864
TGGTATC14650.018.6042652
GTACTTT58050.017.94321814-15
Produced by FastQC (version 0.11.5)