##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931149_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220095 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.33459424033656 31.0 30.0 33.0 25.0 34.0 2 29.073251369873812 31.0 28.0 34.0 23.0 34.0 3 29.100248863224323 31.0 28.0 34.0 23.0 34.0 4 32.3138653976519 35.0 32.0 37.0 22.0 37.0 5 32.44266394005662 35.0 32.0 37.0 24.0 37.0 6 32.73541627723138 35.0 32.0 37.0 25.0 37.0 7 32.407310047543014 35.0 32.0 37.0 23.0 37.0 8 32.587159108056184 35.0 32.0 37.0 25.0 37.0 9 33.701089818228496 37.0 33.0 39.0 23.0 39.0 10-11 33.66868039430745 37.0 32.5 39.0 24.0 39.0 12-13 33.76482357736944 37.0 33.0 39.0 25.0 39.0 14-15 34.56724959967929 38.0 33.0 40.0 24.0 41.0 16-17 34.56456683159955 38.0 33.0 40.0 24.5 41.0 18-19 34.59072787425763 38.0 33.0 40.0 24.5 41.0 20-21 34.77953263261257 38.0 33.0 40.0 25.0 41.0 22-23 34.7058456957923 38.0 33.0 40.0 25.0 41.0 24-25 34.593526853580585 38.0 33.0 40.0 24.5 41.0 26-27 34.238188455899405 38.0 32.5 40.0 22.5 41.0 28-29 34.239542001581015 38.0 32.5 40.0 24.0 41.0 30-31 34.0013080521329 38.0 32.0 40.0 22.0 41.0 32-33 33.788655215204756 38.0 32.0 40.0 22.0 41.0 34-35 33.791083489670484 38.0 32.0 40.0 23.0 41.0 36-37 33.460163416430376 38.0 31.5 40.0 20.0 41.0 38-39 33.280952391676934 37.0 31.0 40.0 18.5 41.0 40-41 33.12958139178729 37.5 31.0 40.0 16.0 41.0 42-43 33.36677597129852 38.0 32.0 40.0 18.0 41.0 44-45 33.44899587630259 38.0 32.0 40.0 19.0 41.0 46-47 33.22961652541896 38.0 32.0 40.0 17.0 41.0 48-49 32.991713642884655 38.0 31.0 40.0 15.0 41.0 50-51 32.47957970267038 37.0 30.5 39.5 13.0 40.5 52-53 32.44670160511149 37.0 31.0 40.0 11.0 40.5 54-55 32.4509005245271 37.0 31.0 40.0 10.0 41.0 56-57 32.23041311295238 37.0 30.0 40.0 8.0 41.0 58-59 32.08239850096505 36.5 30.0 40.0 8.0 41.0 60-61 31.93146261759069 36.0 30.0 40.0 8.0 41.0 62-63 31.790322711415502 36.0 30.0 40.0 7.0 41.0 64-65 31.463665068386714 35.5 30.0 39.0 4.0 41.0 66-67 31.254051290597925 35.0 30.0 39.0 2.0 40.5 68-69 31.05732750175105 35.0 30.0 39.0 2.0 40.0 70-71 30.749582337692758 35.0 29.5 38.5 2.0 40.0 72-73 30.41167179782847 35.0 29.0 38.0 2.0 40.0 74-75 30.00370772421901 34.0 29.0 37.0 2.0 39.5 76-77 29.68026052939176 34.0 29.0 37.0 2.0 39.0 78-79 29.24566043344992 34.0 29.0 36.0 2.0 39.0 80-81 28.83166508640396 34.0 28.0 36.0 2.0 38.5 82-83 28.49557676585912 34.0 28.0 35.0 2.0 37.0 84-85 28.15313556401866 33.5 28.0 35.0 2.0 37.0 86-87 27.827490490271813 33.0 27.5 35.0 2.0 36.0 88-89 27.483878392591308 33.0 27.0 35.0 2.0 36.0 90-91 27.117759149946288 33.0 26.0 35.0 2.0 36.0 92-93 26.762963071400097 33.0 26.0 35.0 2.0 35.0 94-95 26.47206155592441 33.0 25.0 35.0 2.0 35.0 96-97 26.105947493237903 33.0 25.0 35.0 2.0 35.0 98-99 25.748945428010963 32.0 24.0 35.0 2.0 35.0 100-101 25.165308466529584 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.15092444108182 1101 2 5.846758892551243 1101 3 5.392999601950631 1101 4 6.785269722716091 1101 5 6.182803780272582 1101 6 5.752265154463757 1101 7 6.25849103751759 1101 8 5.665808945458473 1101 9 6.3602177396549315 1101 10-11 6.454651628899946 1101 12-13 6.356754303563431 1101 14-15 6.575313016806113 1101 16-17 6.6643581182620935 1101 18-19 6.591726433154271 1101 20-21 5.914756165573962 1101 22-23 6.0099924102234255 1101 24-25 5.979393550915223 1101 26-27 6.120997405605156 1101 28-29 6.0140836056181755 1101 30-31 6.213050449101676 1101 32-33 6.032988070988875 1101 34-35 5.82383321601457 1101 36-37 5.849562290091068 1101 38-39 5.861407930321736 1101 40-41 6.118670259891207 1101 42-43 5.792334111908946 1101 44-45 5.776219625253717 1101 46-47 5.698761671130754 1101 48-49 5.598132861230095 1101 50-51 5.556951634452076 1101 52-53 5.351101346664514 1101 54-55 5.478680328332683 1101 56-57 5.482253225924374 1101 58-59 5.276440607909851 1101 60-61 5.154937083904592 1101 62-63 5.149739845886462 1101 64-65 5.215761755276091 1101 66-67 5.157403445726466 1101 68-69 5.21955507968287 1101 70-71 5.028690651559149 1101 72-73 4.8828225624192925 1101 74-75 5.071563708420744 1101 76-77 4.8713149594957486 1101 78-79 4.9354017347617365 1101 80-81 4.891897730435062 1101 82-83 4.802172724773339 1101 84-85 4.712537742807072 1101 86-87 4.5421598420741205 1101 88-89 4.595264499829536 1101 90-91 4.509932702615366 1101 92-93 4.404997969370012 1101 94-95 4.398638392298437 1101 96-97 4.424620760167077 1101 98-99 4.177708224612292 1101 100-101 4.185370984570614 1102 1 5.319727877243327 1102 2 5.903254527467336 1102 3 5.531085522747208 1102 4 7.0620052899672 1102 5 6.4680698798276985 1102 6 5.967597481709156 1102 7 6.4161683598763695 1102 8 6.023792712487854 1102 9 6.724931993176423 1102 10-11 6.765234562679744 1102 12-13 6.87103124912856 1102 14-15 7.069644673120109 1102 16-17 6.97583243964047 1102 18-19 6.967286760658851 1102 20-21 6.185146540954612 1102 22-23 6.319265399858303 1102 24-25 6.265148472832532 1102 26-27 6.421533095298518 1102 28-29 6.221780562107291 1102 30-31 6.460292315937426 1102 32-33 6.186596756342649 1102 34-35 6.172470861950465 1102 36-37 6.252079872376438 1102 38-39 6.186933719795125 1102 40-41 6.43674998373535 1102 42-43 6.324552635589935 1102 44-45 6.169630956094657 1102 46-47 6.233217091228539 1102 48-49 5.8702341788436705 1102 50-51 5.860938216847522 1102 52-53 5.69500022376112 1102 54-55 6.008566679372262 1102 56-57 5.7942770087122355 1102 58-59 5.819930446487806 1102 60-61 5.711695136216775 1102 62-63 5.610521030332091 1102 64-65 5.6942012477733215 1102 66-67 5.665607147637957 1102 68-69 5.510282275547176 1102 70-71 5.5015477696526 1102 72-73 5.376628680323478 1102 74-75 5.463477517051203 1102 76-77 5.2563944980325985 1102 78-79 5.1991791917609405 1102 80-81 5.244658095986171 1102 82-83 5.125274135712203 1102 84-85 5.072228870015959 1102 86-87 5.038298740289498 1102 88-89 5.111828138750617 1102 90-91 5.176819452350276 1102 92-93 5.305178005933822 1102 94-95 5.187054044311161 1102 96-97 5.052610983208108 1102 98-99 4.803220082456459 1102 100-101 4.786989138826758 1103 1 5.0338881673029725 1103 2 5.726213305248464 1103 3 5.26260371779188 1103 4 6.775656093923079 1103 5 6.321565552999839 1103 6 5.745445056349887 1103 7 6.202002300374783 1103 8 5.7027141387750575 1103 9 6.308184089385865 1103 10-11 6.376426766502583 1103 12-13 6.517252683099894 1103 14-15 6.643555186919983 1103 16-17 6.532609739688738 1103 18-19 6.365149283860248 1103 20-21 5.852058968941201 1103 22-23 6.0485817285962895 1103 24-25 5.7343918314440465 1103 26-27 6.027905088880363 1103 28-29 5.797874809682035 1103 30-31 6.156186869812476 1103 32-33 5.903156151542827 1103 34-35 5.806050782923059 1103 36-37 5.813268703294764 1103 38-39 5.730438450357109 1103 40-41 5.881535409947492 1103 42-43 5.85737908012506 1103 44-45 5.527233394121346 1103 46-47 5.61738394196788 1103 48-49 5.357929480793441 1103 50-51 5.255549976517173 1103 52-53 5.070541695397402 1103 54-55 5.3694074861405845 1103 56-57 5.342944407104746 1103 58-59 5.413103637019635 1103 60-61 5.308352372276218 1103 62-63 5.4429698744022055 1103 64-65 5.260097658013869 1103 66-67 5.377282298131632 1103 68-69 5.412154085394491 1103 70-71 5.342842753230617 1103 72-73 5.123954797585419 1103 74-75 5.123228070473829 1103 76-77 5.047856846147283 1103 78-79 5.060767363162313 1103 80-81 5.022004553383979 1103 82-83 4.919487144523089 1103 84-85 4.980057343365832 1103 86-87 4.895085087583528 1103 88-89 4.9021223967737555 1103 90-91 4.976526967004027 1103 92-93 4.97130005207465 1103 94-95 4.976532972407174 1103 96-97 4.843336998369949 1103 98-99 4.8625059485409885 1103 100-101 4.85554935113208 1104 1 4.718562937030931 1104 2 5.566510874176334 1104 3 5.085635431862496 1104 4 6.688048683604006 1104 5 6.255483639547176 1104 6 5.690534661680545 1104 7 6.2257819597674136 1104 8 5.669179979897727 1104 9 6.358002311177824 1104 10-11 6.345544393966421 1104 12-13 6.320531711880502 1104 14-15 6.432641665583468 1104 16-17 6.392155004614963 1104 18-19 6.566460769813823 1104 20-21 5.948923000174048 1104 22-23 6.160088271228069 1104 24-25 5.839836260457023 1104 26-27 5.890933838228872 1104 28-29 5.90851903329127 1104 30-31 6.186668927990727 1104 32-33 6.052596403295656 1104 34-35 5.860484118699233 1104 36-37 5.871068420813369 1104 38-39 5.671275396278222 1104 40-41 6.085477029777362 1104 42-43 5.977609772404325 1104 44-45 5.887676980097517 1104 46-47 6.072455846088204 1104 48-49 5.77426653459251 1104 50-51 5.656797055996833 1104 52-53 5.482895976871255 1104 54-55 5.76363340155299 1104 56-57 5.651160710360209 1104 58-59 5.519200458642789 1104 60-61 5.416750504988144 1104 62-63 5.418213868213439 1104 64-65 5.490337157337137 1104 66-67 5.63171163652968 1104 68-69 5.409043305234398 1104 70-71 5.343068438537539 1104 72-73 5.226398950881794 1104 74-75 5.262822344280902 1104 76-77 5.230647531128774 1104 78-79 5.279909782057818 1104 80-81 5.247975442221506 1104 82-83 4.910768888445922 1104 84-85 4.880480347969478 1104 86-87 4.852846637828264 1104 88-89 5.008523313813299 1104 90-91 5.00461975896253 1104 92-93 5.096718879930634 1104 94-95 4.900745822030423 1104 96-97 4.936908509670996 1104 98-99 4.7449312065384355 1104 100-101 4.734825264520168 1105 1 4.393867108205882 1105 2 5.167629908288589 1105 3 4.807620062895939 1105 4 6.276143906191582 1105 5 3.618937474266847 1105 6 4.837251293718609 1105 7 5.522617505661525 1105 8 1.0415640696670039 1105 9 0.6563019010199405 1105 10-11 2.3916328660024035 1105 12-13 5.480805405174419 1105 14-15 3.526246870628256 1105 16-17 5.85869136988758 1105 18-19 6.235670595937954 1105 20-21 3.253620236489269 1105 22-23 5.322847153706292 1105 24-25 5.658644181417717 1105 26-27 5.815725808481577 1105 28-29 3.2426355544140293 1105 30-31 5.52774807739009 1105 32-33 5.680720582689968 1105 34-35 5.530816203772012 1105 36-37 5.577939014165789 1105 38-39 5.526722105221484 1105 40-41 5.657450929889716 1105 42-43 2.9173790027947355 1105 44-45 4.9033959923190125 1105 46-47 5.361803139203431 1105 48-49 5.192865945257633 1105 50-51 5.274481621882231 1105 52-53 5.130710363867188 1105 54-55 5.424574461234226 1105 56-57 5.342958847357927 1105 58-59 5.180971595975858 1105 60-61 5.077970600389293 1105 62-63 4.869876350800521 1105 64-65 5.016635936363244 1105 66-67 5.115024140808941 1105 68-69 5.127861798257236 1105 70-71 4.881384538588801 1105 72-73 4.935743891296191 1105 74-75 4.975841890443164 1105 76-77 4.865569284365883 1105 78-79 4.892482704266346 1105 80-81 4.989009285052514 1105 82-83 4.708958984766859 1105 84-85 4.729600936675272 1105 86-87 4.706038185325578 1105 88-89 4.685888719388899 1105 90-91 4.725140584336369 1105 92-93 4.8037306591618325 1105 94-95 4.64918888531998 1105 96-97 4.6342491157782 1105 98-99 4.6055383685664495 1105 100-101 4.696138069995971 1106 1 4.36245381825222 1106 2 4.765481972688534 1106 3 4.754439537218879 1106 4 6.493112921928418 1106 5 6.181275447721266 1106 6 5.384318786131068 1106 7 6.01447956171322 1106 8 5.510438304795315 1106 9 6.22984696913581 1106 10-11 6.1390614537444534 1106 12-13 6.321983926421307 1106 14-15 6.075683142373947 1106 16-17 6.066467606750791 1106 18-19 6.323726593021384 1106 20-21 5.74928164879686 1106 22-23 5.832520825593988 1106 24-25 5.8798949536481935 1106 26-27 6.05702101543935 1106 28-29 5.956513298713709 1106 30-31 6.117231681278941 1106 32-33 6.0030289294464545 1106 34-35 5.602844517234807 1106 36-37 5.823748223054228 1106 38-39 5.646319417611149 1106 40-41 5.711851508805463 1106 42-43 2.5452524804101593 1106 44-45 4.88358154219322 1106 46-47 5.255508583011654 1106 48-49 5.058548687440105 1106 50-51 5.28384445105101 1106 52-53 5.061941332951726 1106 54-55 5.291866076034662 1106 56-57 5.226529896463624 1106 58-59 5.366976474415612 1106 60-61 5.143267830218562 1106 62-63 5.122890177538597 1106 64-65 5.157357520586544 1106 66-67 5.392566969013103 1106 68-69 5.368284056723752 1106 70-71 5.274079610570258 1106 72-73 5.015191203012037 1106 74-75 5.047241469980953 1106 76-77 5.015519466190256 1106 78-79 5.024175708336912 1106 80-81 5.015831793328058 1106 82-83 5.038273483478449 1106 84-85 5.141202090534794 1106 86-87 5.132427375659205 1106 88-89 5.204385279727141 1106 90-91 5.254810858764564 1106 92-93 5.205253850546608 1106 94-95 5.271152804867572 1106 96-97 5.432146201836066 1106 98-99 5.457994026045789 1106 100-101 5.424422611838981 1107 1 3.276256033597278 1107 2 0.1726554639366924 1107 3 1.1232528954612526 1107 4 3.0249471338136225 1107 5 2.9368594513418245 1107 6 4.0801157691591285 1107 7 4.830245993351017 1107 8 4.449144098038445 1107 9 4.844032125567676 1107 10-11 2.7135668768968664 1107 12-13 5.072814621735741 1107 14-15 3.3978166379469172 1107 16-17 1.9351636130324152 1107 18-19 2.5998803496430227 1107 20-21 2.364933963222718 1107 22-23 4.487424158301046 1107 24-25 4.399113645119922 1107 26-27 3.4410460385109367 1107 28-29 4.152680357685988 1107 30-31 3.6437588372168754 1107 32-33 4.1908306177785555 1107 34-35 4.639459289077202 1107 36-37 4.84486094120037 1107 38-39 4.754509506222586 1107 40-41 3.5173171117567357 1107 42-43 4.002787613622733 1107 44-45 4.425816210074622 1107 46-47 4.6291711103814706 1107 48-49 2.533315800295327 1107 50-51 3.9286468005125954 1107 52-53 4.114978363292785 1107 54-55 4.722768296224455 1107 56-57 3.2971496329807835 1107 58-59 2.270160294408779 1107 60-61 3.2765735278667307 1107 62-63 4.366872215377182 1107 64-65 4.490867657314503 1107 66-67 4.5658298645830016 1107 68-69 4.476094320832871 1107 70-71 4.253423006778835 1107 72-73 4.018647140711067 1107 74-75 4.201534114605245 1107 76-77 4.03014474047923 1107 78-79 4.147108792461651 1107 80-81 4.2781580326652 1107 82-83 4.040815420002929 1107 84-85 3.9760697878308413 1107 86-87 4.033095550693677 1107 88-89 4.096593257238105 1107 90-91 4.101645280391473 1107 92-93 4.0965988591076155 1107 94-95 4.1797971467453685 1107 96-97 4.307852381476586 1107 98-99 4.21389914126193 1107 100-101 4.421059802410888 1108 1 -2.017718883500816 1108 2 -1.2782627678894727 1108 3 -1.9663758899907222 1108 4 -3.863558594647998 1108 5 -9.219213879265428 1108 6 -7.134553304726047 1108 7 -5.7046335792938905 1108 8 -6.208562421085215 1108 9 -7.586492408919472 1108 10-11 -3.0145787086472247 1108 12-13 -1.701958889609731 1108 14-15 -6.656491431893912 1108 16-17 -5.460715575303894 1108 18-19 -4.28461990894106 1108 20-21 -4.771175495345027 1108 22-23 -3.678752404188959 1108 24-25 -8.445942699637857 1108 26-27 -9.565290255622426 1108 28-29 -6.37426700679849 1108 30-31 -6.461743959969059 1108 32-33 -2.8632765634778146 1108 34-35 -1.0343874529848165 1108 36-37 -5.01808851163965 1108 38-39 -6.137772878419636 1108 40-41 -3.57730830053848 1108 42-43 -4.229844196598698 1108 44-45 -1.877485325535929 1108 46-47 -2.626858622605255 1108 48-49 -1.645844094224021 1108 50-51 -0.871748924164983 1108 52-53 -2.4146177042608983 1108 54-55 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2313 56-57 -2.0862489785272302 2313 58-59 -1.5617778230022807 2313 60-61 -0.3229882958878001 2313 62-63 -3.6521537109845568 2313 64-65 -4.936152464052469 2313 66-67 -6.069853483038685 2313 68-69 -6.187815533650031 2313 70-71 -4.90639365400439 2313 72-73 -3.9930973274539134 2313 74-75 -5.777999916896377 2313 76-77 -4.148033858365373 2313 78-79 -4.715661355520886 2313 80-81 -6.856292594061308 2313 82-83 -5.35659978841095 2313 84-85 -5.577435902296745 2313 86-87 -4.628009863752688 2313 88-89 -5.781762405153266 2313 90-91 -6.377499323372806 2313 92-93 -5.154481172494865 2313 94-95 -5.727631117303446 2313 96-97 -5.92282051603388 2313 98-99 -5.318896837826642 2313 100-101 -5.016135869618115 2314 1 -12.142364775852089 2314 2 -13.156106359614085 2314 3 -12.136304639239585 2314 4 -25.532113835441198 2314 5 -17.92481092622225 2314 6 -17.24310339486254 2314 7 -16.553547649171133 2314 8 -11.237964086464217 2314 9 -14.851579540862128 2314 10-11 -11.755522137267707 2314 12-13 -13.338601519878424 2314 14-15 -16.3715313494834 2314 16-17 -17.01790932584042 2314 18-19 -15.029061491909559 2314 20-21 -15.637880791937008 2314 22-23 -15.488972165541863 2314 24-25 -17.026593399268727 2314 26-27 -15.989039075263598 2314 28-29 -16.961635021220324 2314 30-31 -17.45659330326033 2314 32-33 -14.806469112194442 2314 34-35 -16.247946556770547 2314 36-37 -16.42717942073056 2314 38-39 -14.791734591871432 2314 40-41 -17.973393801439844 2314 42-43 -17.864018803861125 2314 44-45 -14.8085309088478 2314 46-47 -13.190791149002369 2314 48-49 -12.520468525505674 2314 50-51 -12.679371896093905 2314 52-53 -11.70869874125978 2314 54-55 -12.3587214316957 2314 56-57 -11.182045654969066 2314 58-59 -9.820819856237865 2314 60-61 -10.451287416122405 2314 62-63 -14.385535920173217 2314 64-65 -11.041235553006526 2314 66-67 -12.109442925853934 2314 68-69 -16.045586794647097 2314 70-71 -16.90346110268474 2314 72-73 -14.684935059614226 2314 74-75 -12.110844491676435 2314 76-77 -12.271934151620506 2314 78-79 -12.148945812998894 2314 80-81 -12.574523288098455 2314 82-83 -12.723901841196874 2314 84-85 -13.644151444818739 2314 86-87 -13.5757126985523 2314 88-89 -12.33357081180038 2314 90-91 -12.357704601965182 2314 92-93 -12.852917145124387 2314 94-95 -12.595910687195921 2314 96-97 -12.567493047803186 2314 98-99 -12.88072479481589 2314 100-101 -12.06452296639231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56591.0 3 9562.0 4 6285.0 5 5292.0 6 5547.0 7 6182.0 8 7236.0 9 8452.0 10 9602.0 11 10414.0 12 11714.0 13 12920.0 14 14657.0 15 16303.0 16 17964.0 17 19550.0 18 17887.0 19 16008.0 20 15701.0 21 16939.0 22 20400.0 23 24501.0 24 29912.0 25 37080.0 26 44246.0 27 51127.0 28 57162.0 29 64448.0 30 74650.0 31 84409.0 32 99168.0 33 122692.0 34 151853.0 35 188989.0 36 237845.0 37 295894.0 38 280545.0 39 70279.0 40 89.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98030026284939 20.21390191221353 14.36719257569873 23.43860524923835 2 14.612644275724133 20.69343507096001 41.48881535956561 23.205105293750247 3 17.860496960715643 25.819525741015585 30.831473427938892 25.488503870329872 4 11.336769513662484 17.499395665632434 40.47899496704906 30.684839853656023 5 12.328170543728303 37.74307306151436 36.1641033425313 13.764653052226036 6 29.488197336370163 38.29591168301173 18.79012723045524 13.425763750162863 7 25.887522871744185 32.613145808327346 23.32228034783989 18.177050972088583 8 24.23068702716888 36.04320218887724 21.489765790496527 18.236344993457358 9 24.910868678123993 16.4809642009293 21.865681904774785 36.742485216171914 10-11 23.297800648285058 26.87324700959797 29.87440717461927 19.9545451674977 12-13 24.307438579192638 24.514936120915625 29.451151846123146 21.72647345376859 14-15 21.555893319732576 26.17262004213259 27.317185139187927 24.95430149894691 16-17 20.96655934633803 29.16662157409161 27.999400268751746 21.867418810818613 18-19 20.7721226592523 28.296700383166318 29.919329963262875 21.011846994318507 20-21 21.695785336094335 27.561279966453128 29.93018959167731 20.812745105775225 22-23 21.344454618367433 27.40047852002332 29.666708594650622 21.58835826695863 24-25 21.118284611639105 27.577140177814645 29.81472381021082 21.48985140033543 26-27 21.105610981825354 27.702486656664288 29.70512091195689 21.48678144955347 28-29 21.121160976661155 27.715616811688044 29.767409667320344 21.395812544330457 30-31 21.356043871304646 27.58393736458761 29.68988264653738 21.37013611757037 32-33 21.177387179479666 27.734720989532384 29.59719541114004 21.490696419847907 34-35 21.468031719527282 27.813741369752787 29.519290663253134 21.198936247466797 36-37 21.391318113989698 27.543387216915 29.512688887771343 21.55260578132396 38-39 21.329815734212747 27.795918514616424 29.498987927289523 21.37527782388131 40-41 21.406326229341143 27.649838985595725 29.48105657192172 21.46277821314141 42-43 21.34321036195231 27.695028329799808 29.401579759455963 21.560181548791917 44-45 21.36052670668795 27.874597681536663 29.25632591475255 21.50854969702284 46-47 21.439133855250315 27.877783062569172 29.006336489931723 21.67674659224879 48-49 21.43265809053763 27.86930848591332 29.084583211039412 21.613450212509644 50-51 21.275031397719168 27.98882394467149 28.888087980331008 21.84805667727833 52-53 21.35072982022398 28.193619995771247 28.79285836974883 21.66279181425594 54-55 21.40345994999905 28.064884678874463 28.776115195535873 21.75554017559061 56-57 21.34806583400791 28.142888417506303 28.629782096383305 21.87926365210248 58-59 21.37692596394511 28.11920352328623 28.78475682957565 21.71911368319301 60-61 21.536540378447356 28.177403372132076 28.663821931648314 21.622234317772254 62-63 21.420885142302467 28.163997486594045 28.694402717000848 21.72071465410264 64-65 21.31131775892473 27.98859958695461 28.779781946268063 21.92030070785259 66-67 21.359874239615873 28.126701785283963 28.564160542679478 21.949263432420686 68-69 21.44498321017794 28.021999058598844 28.579069814580006 21.95394791664321 70-71 21.50894443706238 27.80408045601652 28.59864104914429 22.088334057776805 72-73 21.52229972140832 27.944322202428275 28.562291253302224 21.97108682286118 74-75 21.48173839407773 28.036210162177746 28.435337226560126 22.046714217184398 76-77 21.45723493814454 28.085217074044127 28.396645188606794 22.060902799204538 78-79 21.49094971161144 28.017427182170135 28.33734592438612 22.15427718183231 80-81 21.474711667743946 28.124156849143844 28.2866949387301 22.11443654438211 82-83 21.632182406608727 27.99882437463262 28.393131825439905 21.975861393318755 84-85 21.69672919402098 27.96740680015945 28.244579623844924 22.091284381974646 86-87 21.426020057700235 28.070983448906468 28.366128476484114 22.136868016909187 88-89 21.71934083901815 27.870879399304982 28.27356486997178 22.136214891705087 90-91 21.64492960886809 27.886149016145705 28.31730173708783 22.151619637898378 92-93 21.76100572272808 27.829935205475444 28.310680398811762 22.098378672984715 94-95 21.60267916463034 27.81135491949669 28.353471360459796 22.23249455541317 96-97 21.588625711962774 27.759059860051032 28.36889862821186 22.283415799774335 98-99 21.61587679806495 27.75421772491718 28.20280213234118 22.42710334467669 100-101 21.620178415788512 27.680211882824835 28.152579056301647 22.54703064508501 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 160.0 2 140.0 3 182.0 4 164.5 5 161.5 6 226.5 7 429.5 8 728.0 9 937.5 10 1007.5 11 1002.5 12 1089.5 13 1272.0 14 1390.0 15 1536.0 16 1759.5 17 1960.0 18 2227.5 19 2516.5 20 2869.5 21 3498.0 22 4340.5 23 5470.5 24 7090.0 25 9498.5 26 12920.5 27 17149.0 28 21640.0 29 27086.0 30 33342.0 31 39665.5 32 46021.0 33 52579.5 34 59633.5 35 65371.0 36 71604.0 37 78166.5 38 81377.5 39 82686.5 40 83532.0 41 83930.5 42 84381.0 43 85271.0 44 85718.0 45 85772.0 46 84384.0 47 82182.5 48 80664.5 49 78217.0 50 74520.5 51 70020.5 52 66060.5 53 61956.5 54 56971.0 55 51755.5 56 46253.5 57 40343.5 58 34877.5 59 29910.5 60 24576.0 61 19647.5 62 15463.5 63 12020.0 64 9272.0 65 6978.5 66 5279.0 67 4123.5 68 3243.0 69 2497.5 70 1972.5 71 1562.0 72 1208.5 73 972.5 74 777.5 75 622.0 76 499.0 77 365.0 78 273.0 79 213.5 80 160.5 81 136.0 82 96.0 83 71.5 84 69.0 85 59.5 86 45.5 87 35.5 88 29.5 89 26.0 90 18.0 91 13.5 92 12.5 93 9.5 94 5.0 95 3.0 96 3.5 97 2.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025809706341395303 2 0.18913605048432613 3 0.0 4 0.4978615779955362 5 0.2956179803116533 6 0.08981597634335468 7 0.10328386848310546 8 0.10526576565417246 9 0.1035991703057752 10-11 0.14274163943434853 12-13 0.23102614978187871 14-15 0.1158283767136091 16-17 0.10967999117154896 18-19 0.1899243050410005 20-21 0.10371177809958582 22-23 0.09035649375364568 24-25 0.09256360651233393 26-27 0.1931674095027465 28-29 0.10702244723761821 30-31 0.1154680317734151 32-33 0.09722556917609382 34-35 0.13684099103867178 36-37 0.13235920084500888 38-39 0.128958445471928 40-41 0.10274335107281446 42-43 0.10310369601300845 44-45 0.08371263391881878 46-47 0.08963580387325767 48-49 0.09359959821539168 50-51 0.08022179231068942 52-53 0.08765390670219067 54-55 0.2944468592560228 56-57 0.31455861123060047 58-59 0.32766615843015723 60-61 0.1569977861307737 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2220095.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.49789535717052 #Duplication Level Percentage of deduplicated Percentage of total 1 86.89460090629473 64.73464885420239 2 8.556735885515081 12.749176291960968 3 2.0473297532762174 4.575652731635801 4 0.8307910928835563 2.4756875160523406 5 0.43727442928093196 1.6288012337468665 6 0.27504499176931746 1.2294163809206662 7 0.17988549191502748 0.9380763387071201 8 0.12662600742692626 0.754669684062996 9 0.09528577106444447 0.638873046160766 >10 0.5177773020262396 7.06652396715345 >50 0.0269840623829458 1.3693371525704217 >100 0.011417917199600561 1.321879959030523 >500 6.159724122946846E-5 0.03501303866360741 >1k 1.8479172368840536E-4 0.48224380513217596 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4845 0.21823390440499166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3573 0.16093905891414556 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.5043117524250086E-5 0.0 0.0 0.0 6 0.0 4.5043117524250086E-5 0.0 0.0 0.0 7 0.0 4.5043117524250086E-5 0.0 0.0 0.0 8 0.0 4.5043117524250086E-5 0.0 0.0 0.0 9 0.0 4.5043117524250086E-5 0.0 4.5043117524250086E-5 0.0 10-11 0.0 4.5043117524250086E-5 0.0 6.756467628637513E-5 0.0 12-13 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 0.0 14-15 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 2.2521558762125043E-5 16-17 0.0 4.5043117524250086E-5 0.0 1.3512935257275026E-4 4.5043117524250086E-5 18-19 0.0 4.5043117524250086E-5 0.0 1.3512935257275026E-4 4.5043117524250086E-5 20-21 0.0 6.756467628637513E-5 0.0 1.3512935257275026E-4 4.5043117524250086E-5 22-23 0.0 9.008623504850017E-5 0.0 2.0269402885912538E-4 4.5043117524250086E-5 24-25 0.0 1.576509113348753E-4 0.0 3.603449401940007E-4 4.5043117524250086E-5 26-27 0.0 2.0269402885912538E-4 0.0 9.459054680092519E-4 4.5043117524250086E-5 28-29 0.0 2.2521558762125045E-4 0.0 0.0022296343174503797 4.5043117524250086E-5 30-31 0.0 2.2521558762125045E-4 0.0 0.006508730482254138 4.5043117524250086E-5 32-33 0.0 2.2521558762125045E-4 0.0 0.018062290127224285 4.5043117524250086E-5 34-35 0.0 2.2521558762125045E-4 0.0 0.0393001200399082 4.5043117524250086E-5 36-37 0.0 2.2521558762125045E-4 0.0 0.07130325504088789 4.5043117524250086E-5 38-39 0.0 2.2521558762125045E-4 0.0 0.11871113623516111 4.5043117524250086E-5 40-41 0.0 2.2521558762125045E-4 0.0 0.16508302572637656 4.5043117524250086E-5 42-43 0.0 2.2521558762125045E-4 0.0 0.21616192099887618 4.5043117524250086E-5 44-45 0.0 2.2521558762125045E-4 0.0 0.2817447001141843 4.5043117524250086E-5 46-47 0.0 2.2521558762125045E-4 0.0 0.3571919219673032 4.5043117524250086E-5 48-49 0.0 2.2521558762125045E-4 0.0 0.43928300365524897 4.5043117524250086E-5 50-51 0.0 2.2521558762125045E-4 0.0 0.5211939128730978 4.5043117524250086E-5 52-53 0.0 2.2521558762125045E-4 0.0 0.6076316554021337 4.5043117524250086E-5 54-55 0.0 2.2521558762125045E-4 0.0 0.7024924609082044 4.5043117524250086E-5 56-57 0.0 2.2521558762125045E-4 0.0 0.8136363533992914 4.5043117524250086E-5 58-59 0.0 2.2521558762125045E-4 0.0 0.9370544954157367 4.5043117524250086E-5 60-61 0.0 2.2521558762125045E-4 0.0 1.06659850141548 4.5043117524250086E-5 62-63 0.0 2.2521558762125045E-4 0.0 1.1920435837205163 9.008623504850017E-5 64-65 0.0 2.2521558762125045E-4 0.0 1.3313169031054977 1.1260779381062523E-4 66-67 0.0 2.2521558762125045E-4 0.0 1.4892380731455186 1.3512935257275026E-4 68-69 0.0 2.2521558762125045E-4 0.0 1.6664827406034428 1.3512935257275026E-4 70-71 0.0 2.2521558762125045E-4 0.0 1.8580285978753162 1.3512935257275026E-4 72-73 0.0 2.2521558762125045E-4 0.0 2.05466432742743 1.3512935257275026E-4 74-75 0.0 2.2521558762125045E-4 0.0 2.2528540445341303 1.8017247009700034E-4 76-77 0.0 2.2521558762125045E-4 0.0 2.4675745857722307 1.8017247009700034E-4 78-79 0.0 2.7025870514550053E-4 0.0 2.7066184104734257 1.8017247009700034E-4 80-81 0.0 2.7025870514550053E-4 0.0 2.9709989887820116 1.8017247009700034E-4 82-83 0.0 2.7025870514550053E-4 0.0 3.243014375510958 1.8017247009700034E-4 84-85 0.0 2.7025870514550053E-4 0.0 3.5143991585945646 1.8017247009700034E-4 86-87 0.0 2.7025870514550053E-4 0.0 3.809251405908306 1.8017247009700034E-4 88-89 0.0 2.7025870514550053E-4 0.0 4.125994608338832 1.8017247009700034E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2510 0.0 48.57603 1 GTATCAA 4605 0.0 43.712738 1 ATCAACG 5400 0.0 35.80362 3 TCAACGC 5515 0.0 35.063393 4 CAACGCA 5520 0.0 34.92944 5 AACGCAG 5855 0.0 33.47889 6 TATCAAC 6085 0.0 32.846645 2 ACGCAGA 6670 0.0 28.822708 7 CGCAGAG 6805 0.0 27.899382 8 GTACATG 5210 0.0 23.952915 1 GCAGAGT 7735 0.0 23.130615 9 TACATGG 5330 0.0 21.979315 2 ACATGGG 5095 0.0 21.5501 3 GTGGTAT 1545 0.0 21.044374 1 GAGTACT 5090 0.0 20.286701 12-13 AGAGTAC 7025 0.0 20.285265 10-11 CAGAGTA 7360 0.0 19.879135 10-11 CATGGGG 3205 0.0 19.366886 4 TGGTATC 1465 0.0 18.604265 2 GTACTTT 5805 0.0 17.943218 14-15 >>END_MODULE