FastQCFastQC Report
Sat 18 Jun 2016
SRR1931149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220095
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54400.2450345593319205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47040.2118828248340724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30990.13958862120765103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28050.053.728921
GTATCAA55600.043.3525731
ATCAACG68600.034.1983383
TCAACGC69100.034.155524
CAACGCA68750.034.0531125
AACGCAG72850.032.4625326
TATCAAC73550.032.284162
ACGCAGA85100.027.5663957
CGCAGAG87000.026.8552068
GCAGAGT99650.022.9218889
GTGGTAT16250.021.9426781
GTACATG56050.021.7991331
TACATGG56100.021.3324052
AGAGTAC91300.020.70116810-11
TGGTATC16200.020.520372
GAGTACT67050.019.9371112-13
ACATGGG56400.019.6190953
CAGAGTA96500.019.29040510-11
AGTACTT72600.018.9035712-13
GTACTTT71600.018.60383414-15
Produced by FastQC (version 0.11.5)