##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931149_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220095 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13351906112126 31.0 31.0 34.0 28.0 34.0 2 31.14708604811956 31.0 31.0 34.0 28.0 34.0 3 31.2971908859756 31.0 31.0 34.0 28.0 34.0 4 34.934521720917346 35.0 35.0 37.0 32.0 37.0 5 34.81536060393812 35.0 35.0 37.0 32.0 37.0 6 34.942889831291005 35.0 35.0 37.0 32.0 37.0 7 34.782273281098334 35.0 35.0 37.0 32.0 37.0 8 34.85045594895714 35.0 35.0 37.0 32.0 37.0 9 36.22474443661195 38.0 35.0 39.0 32.0 39.0 10-11 36.09936669376761 38.0 35.0 39.0 31.0 39.0 12-13 36.02455750767422 37.0 35.0 39.0 30.0 39.0 14-15 36.943892716302685 38.0 36.0 40.0 31.0 41.0 16-17 36.9801233730989 38.0 36.0 40.0 31.0 41.0 18-19 37.0528454863418 38.5 36.0 40.0 31.0 41.0 20-21 37.040934734774865 39.0 36.0 40.0 31.0 41.0 22-23 36.91584571831386 39.0 36.0 40.0 31.0 41.0 24-25 36.78527337794104 39.0 35.5 40.0 30.0 41.0 26-27 36.5227713228488 38.0 35.0 40.0 30.0 41.0 28-29 36.23279206520442 38.0 35.0 40.0 30.0 41.0 30-31 35.97905292341093 38.0 34.0 40.0 29.0 41.0 32-33 35.815674554467265 38.0 34.0 40.0 29.0 41.0 34-35 35.58209941466469 38.0 34.0 40.0 27.5 41.0 36-37 35.84266033660722 38.0 34.5 40.0 29.0 41.0 38-39 35.84211373837606 38.0 35.0 40.0 29.0 41.0 40-41 35.67927318425563 38.0 34.0 40.0 28.0 41.0 42-43 35.50962233598112 38.0 34.0 40.0 27.0 41.0 44-45 35.2786223112074 38.0 34.0 40.0 27.0 41.0 46-47 35.052366678002514 38.0 33.5 40.0 26.5 41.0 48-49 34.80657584472736 38.0 33.0 40.0 26.0 41.0 50-51 34.47459568171632 38.0 33.0 40.0 24.0 41.0 52-53 34.34851864447242 38.0 33.0 40.0 24.0 41.0 54-55 34.10703956362228 37.5 33.0 40.0 24.0 41.0 56-57 33.88467498012473 37.0 32.5 40.0 23.0 41.0 58-59 33.65186962720064 37.0 32.0 40.0 23.0 41.0 60-61 33.37868537157193 37.0 32.0 40.0 22.0 41.0 62-63 33.116658070938406 36.5 31.5 39.0 21.5 41.0 64-65 32.87837952880395 36.0 31.0 39.0 22.0 41.0 66-67 32.62713059576279 36.0 31.0 39.0 21.0 40.0 68-69 32.29725439677131 35.0 31.0 39.0 20.0 40.0 70-71 31.491969938223363 35.0 30.0 38.0 15.5 40.0 72-73 31.421548402208014 35.0 30.0 37.5 18.0 40.0 74-75 31.100876539067023 34.5 30.0 37.0 17.5 39.0 76-77 30.38076028278069 34.0 29.0 36.0 13.5 39.0 78-79 30.39401444532779 34.0 29.0 36.0 13.0 39.0 80-81 30.118281875325152 34.0 29.0 36.0 10.5 38.0 82-83 29.75339929147176 34.0 29.0 35.5 7.5 37.0 84-85 29.378211292759993 34.0 29.0 35.0 7.0 37.0 86-87 29.030307036410605 34.0 29.0 35.0 2.0 36.0 88-89 28.702944919023736 34.0 29.0 35.0 2.0 36.0 90-91 28.38918289532655 33.0 28.5 35.0 2.0 36.0 92-93 28.026572736752257 33.0 27.5 35.0 2.0 35.0 94-95 27.625051180242288 33.0 27.0 35.0 2.0 35.0 96-97 27.230659724020818 33.0 26.0 35.0 2.0 35.0 98-99 26.73383323686599 33.0 25.5 35.0 2.0 35.0 100-101 26.033174481272198 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8447922606687364 1101 2 0.6885257212066627 1101 3 0.5001285455928901 1101 4 0.8053657498380886 1101 5 0.8113539262399527 1101 6 0.8325812001968842 1101 7 0.7698801149917784 1101 8 0.6502617985321351 1101 9 1.112302515231896 1101 10-11 0.8309838979903432 1101 12-13 1.0039586174781476 1101 14-15 1.3241677847542945 1101 16-17 1.1072230267514485 1101 18-19 0.8492563834696227 1101 20-21 0.7842049459272573 1101 22-23 0.7168090351468592 1101 24-25 0.6221586236493479 1101 26-27 0.9268476988283538 1101 28-29 0.8746264460286781 1101 30-31 1.0267370555368913 1101 32-33 1.1470028216644792 1101 34-35 0.9241887893307705 1101 36-37 1.1274517112189173 1101 38-39 0.8073523165183119 1101 40-41 0.8923344617764855 1101 42-43 0.9145949127296262 1101 44-45 0.9427477175502403 1101 46-47 1.0068436220056114 1101 48-49 0.8580224161900674 1101 50-51 0.7363202769425214 1101 52-53 0.7023808336806283 1101 54-55 0.760140078265259 1101 56-57 0.5812935516582982 1101 58-59 0.8066463306014882 1101 60-61 0.975508756008125 1101 62-63 0.8135911127457689 1101 64-65 0.7577376385297256 1101 66-67 0.4529006260338093 1101 68-69 0.48998904037094704 1101 70-71 0.6886428338967896 1101 72-73 0.14361567945964993 1101 74-75 -0.1565427061394793 1101 76-77 -0.01792728929906673 1101 78-79 0.11882319029542643 1101 80-81 0.10081687451601695 1101 82-83 0.02683249704029933 1101 84-85 0.042469288384154424 1101 86-87 -0.061006908359328094 1101 88-89 -0.1959223311395597 1101 90-91 -0.12302839604864602 1101 92-93 -0.39267196164857054 1101 94-95 -0.32722391510472804 1101 96-97 -0.38163941172564364 1101 98-99 -0.437265355708643 1101 100-101 -0.4514563086080621 1102 1 0.9519910942497951 1102 2 0.819740304053191 1102 3 0.6114281267293116 1102 4 0.9083769710682006 1102 5 0.9500518024852553 1102 6 0.8876976166134156 1102 7 0.871891559524542 1102 8 0.8499267358132556 1102 9 1.2878530048826065 1102 10-11 1.2052523515302482 1102 12-13 1.2556951298156633 1102 14-15 1.540117120702348 1102 16-17 1.4766603456458611 1102 18-19 1.173426493651668 1102 20-21 1.1551351815349022 1102 22-23 0.8172251226349374 1102 24-25 0.6200442846319234 1102 26-27 1.096652702713925 1102 28-29 1.2598280622255942 1102 30-31 1.482424144236397 1102 32-33 1.4774212163787368 1102 34-35 1.590916873288478 1102 36-37 1.5714851647834465 1102 38-39 1.4328400594670825 1102 40-41 1.458265961017446 1102 42-43 1.5206999407109052 1102 44-45 1.3998507727795584 1102 46-47 1.240571392725542 1102 48-49 1.0693226945476013 1102 50-51 1.0079019729309593 1102 52-53 1.1223718324920995 1102 54-55 0.8962421811161221 1102 56-57 0.9973762103555757 1102 58-59 1.0896460137816604 1102 60-61 1.365006725463573 1102 62-63 1.2410427292785045 1102 64-65 1.172238596760998 1102 66-67 0.8270721402528665 1102 68-69 0.8681968571663141 1102 70-71 1.2428948560459112 1102 72-73 0.5899873823633719 1102 74-75 0.5591190101080308 1102 76-77 0.6436304523467378 1102 78-79 0.6657301479288797 1102 80-81 0.6499903764548982 1102 82-83 0.6170348709054849 1102 84-85 0.5943496073736831 1102 86-87 0.6258994582353132 1102 88-89 0.4947706817006292 1102 90-91 0.46117531763244557 1102 92-93 0.3740759131543179 1102 94-95 0.28660529936822954 1102 96-97 0.4177439121909394 1102 98-99 0.4928697945274756 1102 100-101 0.4288106771854494 1103 1 0.7501860324077185 1103 2 0.5149313664141388 1103 3 0.350457184174946 1103 4 0.6412429331105969 1103 5 0.785045953158729 1103 6 0.7233268497036818 1103 7 0.7700396701020509 1103 8 0.6210304034284775 1103 9 0.8683218842591742 1103 10-11 0.8570181875071654 1103 12-13 0.9883208041112681 1103 14-15 1.350500702366837 1103 16-17 1.1839116655800623 1103 18-19 0.9072182926245276 1103 20-21 0.9671771924555017 1103 22-23 0.7878203692741437 1103 24-25 0.5006805539053403 1103 26-27 1.0166453469924477 1103 28-29 0.7005855937565499 1103 30-31 1.0399119415496827 1103 32-33 1.175740045377303 1103 34-35 1.074488419060188 1103 36-37 1.2979528157126836 1103 38-39 0.996320785346569 1103 40-41 1.0543649877189267 1103 42-43 1.185895198914352 1103 44-45 1.1584942210139673 1103 46-47 1.1612840760384628 1103 48-49 1.0587627454809976 1103 50-51 0.9063345296762719 1103 52-53 0.5000980206103876 1103 54-55 0.6575379559318861 1103 56-57 0.8344633370441557 1103 58-59 0.9778973441135292 1103 60-61 0.9680588807115171 1103 62-63 0.97006966661273 1103 64-65 0.8481537633938956 1103 66-67 0.7695201816731441 1103 68-69 0.6744926307140666 1103 70-71 1.1387079593488458 1103 72-73 0.5363702465882412 1103 74-75 0.334888317640587 1103 76-77 0.43768239228671746 1103 78-79 0.5294689897647622 1103 80-81 0.30652145447322354 1103 82-83 0.413396743237751 1103 84-85 0.5569375660031106 1103 86-87 0.4649492729918414 1103 88-89 0.31779796367975877 1103 90-91 0.34269627693721105 1103 92-93 0.10652653877738416 1103 94-95 0.09725855489313417 1103 96-97 -0.015993001002925666 1103 98-99 -0.104683258344199 1103 100-101 -0.22983480620375474 1104 1 0.5756551221688433 1104 2 0.509840730989886 1104 3 0.21376363599449633 1104 4 0.6472343879461206 1104 5 0.5091442240876987 1104 6 0.6239563855107093 1104 7 0.6302566098630962 1104 8 0.5504151813100648 1104 9 0.8224581200306886 1104 10-11 0.6735711381615914 1104 12-13 0.8261243678251162 1104 14-15 1.1809547917102918 1104 16-17 0.9761154585794216 1104 18-19 0.735179978267638 1104 20-21 0.6471246141209903 1104 22-23 0.41671762076918384 1104 24-25 0.4089477724835149 1104 26-27 0.24914778513686997 1104 28-29 0.4719014845133316 1104 30-31 0.8298788043099634 1104 32-33 1.0506575353928227 1104 34-35 0.8108759371957248 1104 36-37 0.9314043877328828 1104 38-39 0.41350946005685074 1104 40-41 0.8354861779741398 1104 42-43 0.8717697586262076 1104 44-45 0.8098011330444876 1104 46-47 0.8627045930429489 1104 48-49 0.7274275673747397 1104 50-51 0.6379485761555301 1104 52-53 0.3600994606914867 1104 54-55 0.6693729498000067 1104 56-57 0.733456253997602 1104 58-59 0.5416717326043639 1104 60-61 0.703290938761647 1104 62-63 -0.014232808840553446 1104 64-65 -0.9735640688586926 1104 66-67 -0.41402443188544424 1104 68-69 0.2183562324850108 1104 70-71 0.7810720830851174 1104 72-73 0.2669118554509424 1104 74-75 0.2506059059506178 1104 76-77 -0.1023912594053229 1104 78-79 -0.07838206587617691 1104 80-81 -0.029516024021649656 1104 82-83 -0.15007335765504948 1104 84-85 -0.35594419001435185 1104 86-87 -0.12124406481376937 1104 88-89 0.012083801327783306 1104 90-91 0.09272792284740916 1104 92-93 0.0361853676788364 1104 94-95 0.15242736980888694 1104 96-97 0.3776785984966864 1104 98-99 0.3300508955107908 1104 100-101 0.13441083729468772 1105 1 -0.5048750790593566 1105 2 -2.0039564058319783 1105 3 -0.571290136960819 1105 4 0.30343664587171304 1105 5 0.5606856879378341 1105 6 -0.01250868228313351 1105 7 -0.08442948567498121 1105 8 -0.13622357490072545 1105 9 0.3961481732214267 1105 10-11 0.8972974588472624 1105 12-13 0.9227836756163725 1105 14-15 1.151386536936144 1105 16-17 0.8096097689672277 1105 18-19 0.604946469930006 1105 20-21 0.8852009404382102 1105 22-23 0.3178868701908968 1105 24-25 -0.22505887385662504 1105 26-27 1.0213499893681401 1105 28-29 0.5830655265391371 1105 30-31 0.88531088234528 1105 32-33 0.9129279504737511 1105 34-35 0.47545006101324105 1105 36-37 0.6858798013737513 1105 38-39 0.4949837599377247 1105 40-41 0.8890943568581946 1105 42-43 1.1094120823321063 1105 44-45 1.100463341282179 1105 46-47 1.068845189872789 1105 48-49 0.9099391684456393 1105 50-51 0.6289918313784568 1105 52-53 0.9174353042078067 1105 54-55 0.0065786417791997565 1105 56-57 0.36078681386573663 1105 58-59 -0.5537778246796208 1105 60-61 -0.013128665193498534 1105 62-63 0.7016452450040518 1105 64-65 1.246407660605179 1105 66-67 0.8328416387530808 1105 68-69 0.7614267818970539 1105 70-71 0.22725428983589424 1105 72-73 -0.21175323067522456 1105 74-75 -0.35737694618046945 1105 76-77 -0.1260949585786051 1105 78-79 -0.5500321002770789 1105 80-81 -0.39493613521337423 1105 82-83 -0.22263237494096444 1105 84-85 -0.1517382170136976 1105 86-87 -0.27462415403848084 1105 88-89 -0.8270628291232782 1105 90-91 -1.0968794700026372 1105 92-93 -0.7217825596552743 1105 94-95 -0.8710660001340145 1105 96-97 -0.8848627584413933 1105 98-99 -1.613284638548329 1105 100-101 -0.9031126758134782 1106 1 0.4445870016753446 1106 2 0.44665932163928446 1106 3 -0.290507930427907 1106 4 0.50129805505847 1106 5 0.251856087908898 1106 6 0.5856163669232899 1106 7 0.5492887393900361 1106 8 0.6103458066836254 1106 9 1.0123497106605015 1106 10-11 0.2608456619883839 1106 12-13 0.40389630104397867 1106 14-15 0.21693294630377125 1106 16-17 -0.08785309481451264 1106 18-19 0.5216513934800773 1106 20-21 0.5977367237015727 1106 22-23 0.6283974679238895 1106 24-25 0.4598178919889122 1106 26-27 0.9745670962505102 1106 28-29 0.803222763659825 1106 30-31 0.4627362091645679 1106 32-33 0.35524175692636817 1106 34-35 -0.8683555954376416 1106 36-37 -0.7992685484468396 1106 38-39 -0.7224498796634578 1106 40-41 0.5621280239795112 1106 42-43 0.7876053750803393 1106 44-45 0.5521267665313303 1106 46-47 0.24909349344821408 1106 48-49 0.21523734339282186 1106 50-51 0.7409968591220135 1106 52-53 0.1413302787339532 1106 54-55 0.877953813159408 1106 56-57 0.5114111679760569 1106 58-59 0.8740422972175423 1106 60-61 1.0370009294449005 1106 62-63 0.9198903029062606 1106 64-65 0.8689846655495828 1106 66-67 0.7294753965206056 1106 68-69 0.7249723163656618 1106 70-71 1.109182021829536 1106 72-73 0.4832737557214628 1106 74-75 0.5290252896174046 1106 76-77 0.5761223713728718 1106 78-79 0.49780132774536767 1106 80-81 0.48378905677145667 1106 82-83 0.44878253394824696 1106 84-85 0.5687467969272326 1106 86-87 0.5535459629291708 1106 88-89 0.5180047281884583 1106 90-91 0.5502047259946039 1106 92-93 0.1658155251421931 1106 94-95 0.48110089102032205 1106 96-97 0.7588580679143817 1106 98-99 0.6727198320544261 1106 100-101 0.5837675183453044 1107 1 0.426618636632611 1107 2 0.39465726321646954 1107 3 0.11700528329948057 1107 4 -1.4777203892134523 1107 5 -0.028109207224268573 1107 6 0.324196374225842 1107 7 0.4752880256774219 1107 8 0.5280289820903192 1107 9 0.8560654060753734 1107 10-11 0.8682898381997788 1107 12-13 0.9089532917054512 1107 14-15 1.2057473621169947 1107 16-17 1.0130896793769821 1107 18-19 0.7505126053262856 1107 20-21 0.16808062033613425 1107 22-23 0.5732782341960103 1107 24-25 0.4465988472058058 1107 26-27 0.7995442905327792 1107 28-29 0.8387052995611057 1107 30-31 1.094328080757741 1107 32-33 1.20513796432369 1107 34-35 1.0096374334570868 1107 36-37 1.1846032007044514 1107 38-39 0.9979243261244619 1107 40-41 0.3665263813770139 1107 42-43 0.13293017340335922 1107 44-45 -0.015087030573411653 1107 46-47 0.4936531333542007 1107 48-49 0.8585929920618653 1107 50-51 -0.8960754745882653 1107 52-53 0.7427163169081297 1107 54-55 1.2723752303279738 1107 56-57 0.9944073932161999 1107 58-59 1.1716589815497755 1107 60-61 1.2498394998218103 1107 62-63 1.4904721529817166 1107 64-65 1.1546983604421612 1107 66-67 1.1611841621877232 1107 68-69 1.2710031613943151 1107 70-71 1.5129650827349828 1107 72-73 1.0723062290547247 1107 74-75 0.9774112533480412 1107 76-77 1.087649672062124 1107 78-79 1.0891515207668157 1107 80-81 1.239020709098881 1107 82-83 1.0416876958393004 1107 84-85 0.9438981949886305 1107 86-87 1.1210892908387606 1107 88-89 1.251730913642664 1107 90-91 1.25817411315888 1107 92-93 1.25403480625398 1107 94-95 1.326114769422123 1107 96-97 1.2651041611058709 1107 98-99 1.2756517552145183 1107 100-101 1.2397839591408335 1108 1 0.5124648610343847 1108 2 0.4708655102525938 1108 3 0.22144741828381598 1108 4 0.4888344293815692 1108 5 0.586947915008011 1108 6 0.5185189897287827 1108 7 0.6151463383314066 1108 8 0.4029591693091277 1108 9 0.7595321058093134 1108 10-11 0.6927461842369453 1108 12-13 0.8170275119797168 1108 14-15 0.9330325589533572 1108 16-17 0.7849957614418699 1108 18-19 0.5825872172902464 1108 20-21 0.4078944742427737 1108 22-23 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1.1348063367803434 2311 74-75 1.3040195394305236 2311 76-77 1.4379667875663955 2311 78-79 1.2173128368694144 2311 80-81 1.2650805424947436 2311 82-83 1.4107823926936582 2311 84-85 1.6589441033396888 2311 86-87 1.5292751741285358 2311 88-89 1.4597473660741898 2311 90-91 1.5325007131141817 2311 92-93 1.636303628505555 2311 94-95 2.0343038701171388 2311 96-97 2.1904850645417753 2311 98-99 2.47178736424922 2311 100-101 2.3704660547977383 2312 1 -0.014969724240827986 2312 2 -0.1326435823179999 2312 3 -0.2758765940223782 2312 4 0.2046116385563863 2312 5 -0.9513916628042907 2312 6 -0.19770599427926072 2312 7 0.29762829026415005 2312 8 -2.123305718442552 2312 9 -5.043518550622505 2312 10-11 -0.3855390209170366 2312 12-13 -5.393978762960117 2312 14-15 -1.824777608886052 2312 16-17 -2.4510196517598715 2312 18-19 -2.583608828888444 2312 20-21 -3.6757152915131144 2312 22-23 -2.0322400128748797 2312 24-25 -2.560294023745122 2312 26-27 -2.931844285506493 2312 28-29 -5.99457732411722 2312 30-31 -6.8088779741431615 2312 32-33 -1.816849714070635 2312 34-35 0.6638613235880371 2312 36-37 1.1679887845939092 2312 38-39 1.0389894810270448 2312 40-41 -2.6676110221765867 2312 42-43 -5.187857283101209 2312 44-45 -3.851797598913773 2312 46-47 -0.06987457765119842 2312 48-49 -1.6276691740528761 2312 50-51 -7.218950291781361 2312 52-53 -1.6623161260645212 2312 54-55 0.9046938102335034 2312 56-57 1.5137966920781736 2312 58-59 1.4337288600147033 2312 60-61 1.618047487853211 2312 62-63 1.688093982227457 2312 64-65 1.3611432494132316 2312 66-67 0.929953598554107 2312 68-69 1.2271219396558415 2312 70-71 1.7502123049105087 2312 72-73 1.3436177320216558 2312 74-75 1.005332801869617 2312 76-77 1.307547468293361 2312 78-79 1.1682917819915808 2312 80-81 1.0501731910703391 2312 82-83 0.29175031245750205 2312 84-85 0.6714276542163837 2312 86-87 1.1938081968549987 2312 88-89 1.018943759715043 2312 90-91 1.295161203209144 2312 92-93 1.5112529474229355 2312 94-95 1.4122727145884575 2312 96-97 2.016027441039977 2312 98-99 1.9511157065332476 2312 100-101 1.900029130517055 2313 1 -0.05744284252039833 2313 2 0.06305534241318256 2313 3 -0.4742636907965725 2313 4 -1.1529152431640455 2313 5 -2.085262630546225 2313 6 -1.4595339512685044 2313 7 -1.0077480538218708 2313 8 -4.453950879732879 2313 9 -1.4526583355687421 2313 10-11 -1.165915365003059 2313 12-13 -2.403387365110653 2313 14-15 -3.181766856197882 2313 16-17 -6.023062662512558 2313 18-19 -3.4938238826518813 2313 20-21 -4.3824357216206415 2313 22-23 -1.9249819483587487 2313 24-25 -0.3791112280462059 2313 26-27 -3.547166866151649 2313 28-29 -3.639200980031198 2313 30-31 -2.9268887268313293 2313 32-33 -7.72625831622117 2313 34-35 -2.977267708670034 2313 36-37 -3.3978714304598583 2313 38-39 -1.0386986910159592 2313 40-41 0.5646470423395442 2313 42-43 -1.1768357777248681 2313 44-45 -3.4348621150428045 2313 46-47 -0.8290143625974338 2313 48-49 0.5685673850869151 2313 50-51 1.6466411060681025 2313 52-53 1.5212860244731132 2313 54-55 -1.8910051144976805 2313 56-57 -3.235128039104623 2313 58-59 0.17915896754158922 2313 60-61 -6.80157616806077 2313 62-63 -0.5487339747617952 2313 64-65 -0.39584599789859354 2313 66-67 -2.253110917574922 2313 68-69 -4.023953329161369 2313 70-71 -3.4884973725088457 2313 72-73 0.8721123556775723 2313 74-75 2.8502252749878956 2313 76-77 1.9312033822718533 2313 78-79 2.6621089862926546 2313 80-81 2.60877534160797 2313 82-83 2.88234171030696 2313 84-85 3.112287869270151 2313 86-87 2.7951522828765043 2313 88-89 -0.46304548759678 2313 90-91 1.8540321709510792 2313 92-93 2.559102409788526 2313 94-95 2.609584542545445 2313 96-97 2.8119951829754584 2313 98-99 3.0051479645977643 2313 100-101 3.24384633481813 2314 1 -28.584324562950506 2314 2 -14.693054139502749 2314 3 -4.886287151207128 2314 4 -2.890940658608816 2314 5 -0.674705445795496 2314 6 -0.35640589652754784 2314 7 -0.5756854927270538 2314 8 -5.4725237047573145 2314 9 -2.937995578970501 2314 10-11 -0.28321741778897547 2314 12-13 0.20927148141916518 2314 14-15 -0.2611901211050167 2314 16-17 -0.009621802297509419 2314 18-19 -3.5705589755160076 2314 20-21 -2.398075995325435 2314 22-23 -6.834805995279574 2314 24-25 -1.563373202630757 2314 26-27 -4.660070091958104 2314 28-29 -3.1101198461113526 2314 30-31 -4.882411698483338 2314 32-33 -3.0119376906102246 2314 34-35 -2.744863016587921 2314 36-37 -6.1263660541157705 2314 38-39 -8.303605826890838 2314 40-41 -6.848109555900926 2314 42-43 -4.237930596884201 2314 44-45 -5.79947599578572 2314 46-47 -8.430676141678568 2314 48-49 -5.900640826056783 2314 50-51 -2.0769659320550673 2314 52-53 -2.9965536627214107 2314 54-55 -5.5009757889844835 2314 56-57 -2.2835151358788153 2314 58-59 -1.2986025182844187 2314 60-61 -0.49854586503046505 2314 62-63 0.15776651399676211 2314 64-65 -0.911730650880024 2314 66-67 -0.5593181512015093 2314 68-69 0.8653428585219167 2314 70-71 1.1214733019779608 2314 72-73 0.4258757965377882 2314 74-75 1.3446045516252347 2314 76-77 -1.4099500879138738 2314 78-79 -4.36135142426453 2314 80-81 -3.6871679623998475 2314 82-83 -2.806074711980429 2314 84-85 -0.8234403809742297 2314 86-87 0.7536078058481586 2314 88-89 0.899124600379757 2314 90-91 1.557600108389984 2314 92-93 2.0314875515285067 2314 94-95 2.0594867908347894 2314 96-97 1.7308612631318638 2314 98-99 1.9274353546270895 2314 100-101 2.089887390536603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 24.0 4 57.0 5 105.0 6 251.0 7 627.0 8 1566.0 9 2754.0 10 4279.0 11 6053.0 12 7991.0 13 10269.0 14 12273.0 15 14699.0 16 16561.0 17 18179.0 18 16903.0 19 14830.0 20 14691.0 21 15539.0 22 16849.0 23 19318.0 24 23385.0 25 28644.0 26 35706.0 27 45538.0 28 56172.0 29 69543.0 30 85390.0 31 102619.0 32 122715.0 33 147272.0 34 177441.0 35 215552.0 36 266054.0 37 321470.0 38 275311.0 39 53426.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.425795853656446 21.906576730172027 15.493258144764448 23.174369271407073 2 15.020753616399299 22.195311461896903 40.08040196478079 22.703532956923013 3 18.190163709941874 26.530659128009425 31.355967322875788 23.92320983917291 4 12.706478301761011 18.38071283660828 39.051740291375836 29.861068570254872 5 12.024512519762713 38.61582188109491 36.42685656887784 12.932809030264538 6 29.558239624880915 39.49912053313034 17.950898497586813 12.991741344401927 7 25.687369234199437 33.30001644073789 23.21157427947903 17.801040045583637 8 24.297834101693848 36.971120605199324 20.75501273594148 17.976032557165347 9 24.880376740634976 16.40137021163509 21.36431098669201 37.35394206103793 10-11 23.42564169551303 27.454072911294336 29.487994883101848 19.632290510090787 12-13 24.183447104741013 24.89596165929836 29.464662548224286 21.455928687736336 14-15 21.402642679705146 26.723045635434516 27.110574097054407 24.763737587805927 16-17 20.88991687292661 29.705845921007885 27.835407944254637 21.56882926181087 18-19 20.700465520619613 28.69291629412255 29.921692540184093 20.684925645073747 20-21 21.51320101166842 27.818201473360375 29.99324353237136 20.675353982599844 22-23 21.262671191998542 27.679153369563014 29.71893094664868 21.339244491789767 24-25 21.134062882507624 27.869620903831866 29.855495743395473 21.140820470265037 26-27 21.233956600159644 27.990000522542296 29.6251074364976 21.150935440800463 28-29 21.117304141928138 27.933909873045497 29.827157457414373 21.12162852761199 30-31 21.454528092091767 27.71914912593047 29.716900073939893 21.109422708037872 32-33 21.291592372955222 27.92932306070047 29.55203960589593 21.22704496044838 34-35 21.40533014479286 27.898993416090345 29.625363761402703 21.0703126777141 36-37 21.4591254913416 27.754986949732878 29.53907524066251 21.246812318263007 38-39 21.297385679795322 27.947506396165906 29.537021188425644 21.21808673561313 40-41 21.475523628188515 27.83358760814562 29.531710929402593 21.15917783426327 42-43 21.41456928346339 27.820746932075956 29.40941448640312 21.355269298057536 44-45 21.461687936132144 27.96105913109594 29.219164680255343 21.358088252516573 46-47 21.557527825870483 27.94286248539853 28.974545847775225 21.52506384095576 48-49 21.529669297950807 27.93915579658891 29.04216421844013 21.489010687020148 50-51 21.541598106615574 28.066200833990756 28.783184943085764 21.6090161163079 52-53 21.580072511259786 28.159034696669337 28.702779057768023 21.558113734302854 54-55 21.62618173363439 28.070930377690654 28.765540352083512 21.537347536591444 56-57 21.646052868946512 28.202702206615136 28.557915511393873 21.593329413044472 58-59 21.54016037303261 28.16813763865529 28.758838669341653 21.53286331897045 60-61 21.622176156654163 28.195325816297483 28.673766589910738 21.508731437137616 62-63 21.60826712155685 28.17238830408514 28.61276662448993 21.606577949868086 64-65 21.49982870645557 27.987946159995154 28.681837050624733 21.830388082924546 66-67 21.484833551504842 28.14698513400656 28.51201324704973 21.856168067438873 68-69 21.59315669679677 28.09169118200176 28.49186433211309 21.82328778908839 70-71 21.657854219388945 27.814975544670506 28.4999635129547 22.02720672298585 72-73 21.576187990120673 27.92103924839267 28.55259643178412 21.950176329702533 74-75 21.51501181157248 28.028549569529048 28.38655654093556 22.06988207796291 76-77 21.409714449156457 28.12208486573773 28.324396028097897 22.14380465700792 78-79 21.45421704927041 28.074676984543455 28.217441145536565 22.253664820649565 80-81 21.434969175792236 28.173796596939 28.14879760478125 22.242436622487517 82-83 21.555501753872313 28.022071202148563 28.271926219124243 22.150500824854877 84-85 21.566807178703428 27.987975286637575 28.12125790140915 22.323959633249846 86-87 21.272293090102067 28.123949209354997 28.230500647619237 22.3732570529237 88-89 21.512174994515767 27.956834253678263 28.150302634998813 22.380688116807153 90-91 21.446547141314714 27.969954852551215 28.2148807756911 22.368617230442975 92-93 21.48741903226097 27.893397356780568 28.19148025693462 22.427703354023837 94-95 21.331674831925838 27.842801813360936 28.200739929457384 22.624783425255842 96-97 21.276141477593406 27.89638945931301 28.21417037110775 22.61329869198584 98-99 21.209639694970768 27.862937290362222 28.14878677254719 22.778636242119827 100-101 21.173577402766817 27.848122362641124 28.089786416723424 22.888513817868635 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 165.5 2 141.0 3 184.5 4 154.0 5 155.5 6 256.5 7 466.5 8 763.5 9 981.0 10 1045.0 11 1044.0 12 1111.0 13 1280.0 14 1481.0 15 1662.5 16 1803.5 17 2031.0 18 2295.5 19 2566.0 20 2964.0 21 3615.5 22 4467.0 23 5657.5 24 7475.0 25 9905.5 26 13366.5 27 17534.0 28 22023.5 29 27583.5 30 33663.0 31 39758.5 32 46464.0 33 53220.0 34 59842.0 35 65375.5 36 72014.0 37 78712.0 38 81678.5 39 82693.5 40 83418.0 41 83887.5 42 84308.5 43 85123.0 44 85347.5 45 85166.5 46 84110.0 47 82298.5 48 80753.5 49 78280.5 50 74136.5 51 69466.0 52 65495.5 53 61429.5 54 56693.0 55 51644.0 56 46026.0 57 40115.5 58 34856.5 59 30067.0 60 24675.0 61 19458.0 62 15214.0 63 11618.0 64 8823.0 65 6817.5 66 5249.0 67 3962.0 68 3036.0 69 2404.0 70 1903.5 71 1460.5 72 1131.0 73 940.5 74 735.0 75 562.5 76 439.0 77 324.5 78 241.0 79 174.5 80 136.5 81 112.0 82 86.0 83 65.5 84 52.5 85 37.5 86 30.0 87 23.0 88 13.5 89 9.0 90 7.0 91 5.0 92 3.5 93 2.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1072927059427637 2 0.0 3 0.0031980613442217564 4 3.153018226697506E-4 5 2.2521558762125042E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.016575867248924032 26-27 0.008062718036840765 28-29 0.005697954366817636 30-31 0.002184591199926129 32-33 9.68427026771377E-4 34-35 0.0027701517277413804 36-37 0.001148599496868377 38-39 6.756467628637512E-4 40-41 0.002522414581358005 42-43 0.0012161641731547525 44-45 4.95474292766751E-4 46-47 0.03209322123602819 48-49 0.017701945187030287 50-51 0.08310455183224141 52-53 0.0011711210556305024 54-55 0.03567414907920607 56-57 9.233839092471268E-4 58-59 9.459054680092517E-4 60-61 0.0031980613442217564 62-63 0.0032431044617460066 64-65 0.009977050531621396 66-67 0.005810562160628262 68-69 0.002522414581358005 70-71 0.005810562160628262 72-73 0.00477457045757051 74-75 0.0011936426143926275 76-77 0.0 78-79 0.0 80-81 2.477371463833755E-4 82-83 3.378233814318756E-4 84-85 2.2521558762125043E-5 86-87 0.0018242462597321285 88-89 0.004234053047279508 90-91 0.0016215522308730033 92-93 0.0033782338143187565 94-95 8.332976741986266E-4 96-97 5.405174102910011E-4 98-99 0.002049461847353379 100-101 0.0031980613442217564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2220095.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.25371182344055 #Duplication Level Percentage of deduplicated Percentage of total 1 85.28133476711082 60.76611651414077 2 9.49218902339662 13.527074024934569 3 2.333136075027838 4.987338166047202 4 0.9457712137064302 2.695588380493744 5 0.5046500173347336 1.7979093453431696 6 0.32551630599242876 1.3916547036609248 7 0.21617726728054687 1.078240289391088 8 0.14454202170856315 0.8239324448959559 9 0.10560412304736692 0.6772217175885816 >10 0.5958802495326851 7.860632160127288 >50 0.03936702525935024 1.909167446316692 >100 0.015451019514742089 1.7824274642627425 >500 1.269636959486463E-4 0.05355607685068601 >1k 1.9044554392196264E-4 0.40307280474183094 >5k 6.348184797398755E-5 0.2460684612047537 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5440 0.2450345593319205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4704 0.2118828248340724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3099 0.13958862120765103 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.008623504850017E-5 0.0 10-11 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 0.0 12-13 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 0.0 14-15 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 0.0 16-17 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 0.0 18-19 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 0.0 20-21 0.0 4.5043117524250086E-5 0.0 9.008623504850017E-5 0.0 22-23 0.0 4.5043117524250086E-5 0.0 1.576509113348753E-4 0.0 24-25 0.0 4.5043117524250086E-5 0.0 3.153018226697506E-4 0.0 26-27 0.0 4.5043117524250086E-5 0.0 9.233839092471269E-4 0.0 28-29 0.0 4.5043117524250086E-5 0.0 0.0022071127586882544 0.0 30-31 0.0 4.5043117524250086E-5 0.0 0.006441165805967763 0.0 32-33 0.0 4.5043117524250086E-5 0.0 0.01826498415608341 0.0 34-35 0.0 4.5043117524250086E-5 0.0 0.0406514135656357 0.0 36-37 0.0 4.5043117524250086E-5 0.0 0.0740959283273914 0.0 38-39 0.0 4.5043117524250086E-5 0.0 0.12341814201644524 0.0 40-41 0.0 4.5043117524250086E-5 0.0 0.17240253232406721 0.0 42-43 0.0 4.5043117524250086E-5 0.0 0.2243597683882897 0.0 44-45 0.0 4.5043117524250086E-5 0.0 0.2919920093509512 0.0 46-47 0.0 4.5043117524250086E-5 0.0 0.3702093829318115 2.2521558762125043E-5 48-49 0.0 4.5043117524250086E-5 0.0 0.45462018517225616 4.5043117524250086E-5 50-51 0.0 4.5043117524250086E-5 0.0 0.5397516772930888 4.5043117524250086E-5 52-53 0.0 4.5043117524250086E-5 0.0 0.6274506271128037 4.5043117524250086E-5 54-55 0.0 4.5043117524250086E-5 0.0 0.7239555064085096 4.5043117524250086E-5 56-57 0.0 4.5043117524250086E-5 0.0 0.836608343336659 4.5043117524250086E-5 58-59 2.2521558762125043E-5 4.5043117524250086E-5 0.0 0.960341787175774 4.5043117524250086E-5 60-61 4.5043117524250086E-5 4.5043117524250086E-5 0.0 1.0874309432704456 4.5043117524250086E-5 62-63 4.5043117524250086E-5 4.5043117524250086E-5 0.0 1.209069882144683 4.5043117524250086E-5 64-65 4.5043117524250086E-5 4.5043117524250086E-5 0.0 1.3442217562761953 4.5043117524250086E-5 66-67 4.5043117524250086E-5 4.5043117524250086E-5 0.0 1.4975034852112183 4.5043117524250086E-5 68-69 4.5043117524250086E-5 4.5043117524250086E-5 0.0 1.6688024611559413 4.5043117524250086E-5 70-71 4.5043117524250086E-5 4.5043117524250086E-5 0.0 1.855686355764055 4.5043117524250086E-5 72-73 4.5043117524250086E-5 4.5043117524250086E-5 0.0 2.05329051234294 4.5043117524250086E-5 74-75 4.5043117524250086E-5 4.5043117524250086E-5 0.0 2.250106414365151 4.5043117524250086E-5 76-77 4.5043117524250086E-5 4.5043117524250086E-5 0.0 2.465502602366115 4.5043117524250086E-5 78-79 4.5043117524250086E-5 4.5043117524250086E-5 0.0 2.703240176659107 4.5043117524250086E-5 80-81 4.5043117524250086E-5 9.008623504850017E-5 0.0 2.965638857796626 6.756467628637513E-5 82-83 4.5043117524250086E-5 1.3512935257275026E-4 0.0 3.2357849551483158 9.008623504850017E-5 84-85 4.5043117524250086E-5 1.3512935257275026E-4 0.0 3.504377064945419 9.008623504850017E-5 86-87 4.5043117524250086E-5 1.3512935257275026E-4 0.0 3.7973375013231414 1.1260779381062523E-4 88-89 4.5043117524250086E-5 1.3512935257275026E-4 0.0 4.113495143225853 1.3512935257275026E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2805 0.0 53.72892 1 GTATCAA 5560 0.0 43.352573 1 ATCAACG 6860 0.0 34.198338 3 TCAACGC 6910 0.0 34.15552 4 CAACGCA 6875 0.0 34.053112 5 AACGCAG 7285 0.0 32.462532 6 TATCAAC 7355 0.0 32.28416 2 ACGCAGA 8510 0.0 27.566395 7 CGCAGAG 8700 0.0 26.855206 8 GCAGAGT 9965 0.0 22.921888 9 GTGGTAT 1625 0.0 21.942678 1 GTACATG 5605 0.0 21.799133 1 TACATGG 5610 0.0 21.332405 2 AGAGTAC 9130 0.0 20.701168 10-11 TGGTATC 1620 0.0 20.52037 2 GAGTACT 6705 0.0 19.93711 12-13 ACATGGG 5640 0.0 19.619095 3 CAGAGTA 9650 0.0 19.290405 10-11 AGTACTT 7260 0.0 18.90357 12-13 GTACTTT 7160 0.0 18.603834 14-15 >>END_MODULE