FastQCFastQC Report
Sat 18 Jun 2016
SRR1931148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778490
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47690.2681488228778346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.2053989620408324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.11251117521043132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.047.5231251
GTATCAA51750.041.2903981
ATCAACG59650.035.2094543
CAACGCA60100.035.047915
TATCAAC62550.034.0848662
TCAACGC62550.033.8092734
AACGCAG63200.033.6060456
ACGCAGA71050.029.5009547
CGCAGAG71250.029.4848528
GCAGAGT77650.025.8304849
GTACATG54400.025.3384671
ACATGGG53950.023.4812563
TACATGG56950.023.2826522
CAACGCG1050.002162083822.6809314
GAGTACT43050.022.5752612-13
GTACTAG7500.022.2581041
GTGGTAT14200.021.8326471
CTGTGCG7600.021.2631139
GCATCGT5400.021.124148
AGAGTAC68800.021.1049510-11
Produced by FastQC (version 0.11.5)