##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931148_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1778490 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.031053309267975 31.0 28.0 33.0 25.0 34.0 2 28.82697962878622 31.0 28.0 33.0 22.0 34.0 3 28.79876187102542 31.0 28.0 34.0 22.0 34.0 4 32.09350347766926 35.0 32.0 37.0 20.0 37.0 5 32.16466777997065 35.0 32.0 37.0 22.0 37.0 6 32.44565333513261 35.0 32.0 37.0 25.0 37.0 7 32.190464944981414 35.0 32.0 37.0 22.0 37.0 8 32.31510607312945 35.0 32.0 37.0 23.0 37.0 9 33.30282543056188 37.0 32.0 39.0 20.0 39.0 10-11 33.30432923435049 37.0 32.0 39.0 21.5 39.0 12-13 33.30322014742843 37.0 32.0 39.0 22.5 39.0 14-15 34.08437494728675 38.0 32.0 40.0 22.0 41.0 16-17 34.07908815905628 38.0 32.0 40.0 21.5 41.0 18-19 34.07171701836951 38.0 32.0 40.0 21.0 41.0 20-21 34.257951127079714 38.0 32.0 40.0 24.0 41.0 22-23 34.13705362414183 38.0 32.0 40.0 23.0 41.0 24-25 34.0080933825886 38.0 32.0 40.0 21.5 41.0 26-27 33.66410241272091 38.0 32.0 40.0 18.5 41.0 28-29 33.57168243847309 38.0 32.0 40.0 19.0 41.0 30-31 33.31351624130583 37.5 31.5 40.0 17.5 41.0 32-33 33.116952864508654 37.0 31.0 40.0 17.5 41.0 34-35 33.036377488768565 37.0 31.0 40.0 18.0 41.0 36-37 32.70978948433783 37.0 30.5 40.0 16.0 41.0 38-39 32.51306332900381 37.0 30.0 40.0 15.0 41.0 40-41 32.388666509229736 36.5 30.0 40.0 14.0 41.0 42-43 32.58492119719537 37.0 30.0 40.0 14.0 41.0 44-45 32.5765331826437 37.0 30.5 40.0 12.0 41.0 46-47 32.3230431433407 37.0 30.0 40.0 9.0 41.0 48-49 32.04519395667111 37.0 30.0 40.0 8.0 41.0 50-51 31.523101619913522 36.5 30.0 39.5 7.0 40.5 52-53 31.432883513542386 36.0 30.0 39.5 6.0 40.5 54-55 31.402780729720156 36.0 30.0 40.0 2.0 41.0 56-57 31.160873831171386 36.0 29.0 40.0 2.0 41.0 58-59 30.95565311022272 35.5 29.0 39.5 2.0 41.0 60-61 30.732286377769906 35.0 29.0 39.0 2.0 41.0 62-63 30.58723214637136 35.0 29.0 39.0 2.0 41.0 64-65 30.25108041091038 35.0 28.0 39.0 2.0 40.0 66-67 30.01260113916862 35.0 28.0 39.0 2.0 40.0 68-69 29.78451776507037 34.0 28.0 38.0 2.0 40.0 70-71 29.496732340356147 34.0 28.0 38.0 2.0 40.0 72-73 29.14608516213192 34.0 28.0 37.0 2.0 40.0 74-75 28.720041158510874 34.0 27.0 36.5 2.0 39.0 76-77 28.429778351298012 34.0 27.0 36.0 2.0 39.0 78-79 28.020007984301287 33.5 26.0 36.0 2.0 39.0 80-81 27.613329284955213 33.0 26.0 35.0 2.0 37.5 82-83 27.263867381880132 33.0 26.0 35.0 2.0 37.0 84-85 26.94158780763457 33.0 25.5 35.0 2.0 36.5 86-87 26.609993027793237 33.0 25.0 35.0 2.0 36.0 88-89 26.26841955816451 32.5 24.5 35.0 2.0 36.0 90-91 25.8795528791278 32.0 23.5 35.0 2.0 35.5 92-93 25.50480632446626 32.0 21.0 35.0 2.0 35.0 94-95 25.19475763147389 32.0 19.5 35.0 2.0 35.0 96-97 24.809500756259524 32.0 17.5 35.0 2.0 35.0 98-99 24.42228435358085 32.0 4.5 34.5 2.0 35.0 100-101 23.81952133551496 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.969600942964028 1101 2 5.743755542210678 1101 3 5.353739733970933 1101 4 6.519802356062499 1101 5 5.987331514660966 1101 6 5.530210156447563 1101 7 6.067506004475501 1101 8 5.4289725761614385 1101 9 6.202935814563233 1101 10-11 6.278473831027892 1101 12-13 6.176708698246134 1101 14-15 6.3831792618200005 1101 16-17 6.283026976262377 1101 18-19 6.35492966068561 1101 20-21 5.687093202026734 1101 22-23 5.745512612875753 1101 24-25 5.70776777509106 1101 26-27 5.805937387430426 1101 28-29 5.673422591024231 1101 30-31 5.653123231418057 1101 32-33 5.55586383721959 1101 34-35 5.538561230426829 1101 36-37 5.5759304516767045 1101 38-39 5.601268510553961 1101 40-41 5.502522247414738 1101 42-43 5.339910869943324 1101 44-45 5.45978409454235 1101 46-47 5.389020957076269 1101 48-49 5.206773015189423 1101 50-51 5.211406544534391 1101 52-53 4.971980060168146 1101 54-55 5.143166323864456 1101 56-57 5.1643776134890444 1101 58-59 4.860805543146398 1101 60-61 4.930401636524238 1101 62-63 4.924703370988901 1101 64-65 4.852215269777545 1101 66-67 4.763695357894434 1101 68-69 4.822276572402302 1101 70-71 4.538309748827569 1101 72-73 4.361343207551059 1101 74-75 4.519289893145718 1101 76-77 4.4077241170533945 1101 78-79 4.404518112816742 1101 80-81 4.216054482996906 1101 82-83 4.1102087702568575 1101 84-85 4.129978122637311 1101 86-87 4.087465380293146 1101 88-89 4.186915355707836 1101 90-91 3.964480318316504 1101 92-93 4.016744451634942 1101 94-95 3.864522061939102 1101 96-97 3.8127807176652375 1101 98-99 3.4868427191859404 1101 100-101 3.5232466839064536 1102 1 5.077631011630501 1102 2 5.7666040628231165 1102 3 5.535431634708665 1102 4 6.757069826718968 1102 5 6.330773166298314 1102 6 5.761671902467516 1102 7 6.227256192135119 1102 8 5.718723982367745 1102 9 6.589516415094536 1102 10-11 6.644549730741499 1102 12-13 6.683533458224954 1102 14-15 6.919584199946232 1102 16-17 6.808714051760589 1102 18-19 6.7858097389190455 1102 20-21 6.097947483695169 1102 22-23 6.272232372466075 1102 24-25 6.085597430478526 1102 26-27 6.287125131589022 1102 28-29 6.20856957650744 1102 30-31 6.291335777451565 1102 32-33 6.244932437975436 1102 34-35 6.289295610181426 1102 36-37 6.181342014110285 1102 38-39 6.099115430968144 1102 40-41 6.278335113898649 1102 42-43 6.096930006945286 1102 44-45 5.9903379976056 1102 46-47 6.139928791475775 1102 48-49 5.796600186932277 1102 50-51 5.703309798088277 1102 52-53 5.366746573272831 1102 54-55 5.8211860691128905 1102 56-57 5.641172727869851 1102 58-59 5.519375263698727 1102 60-61 5.618479569088635 1102 62-63 5.709605748626789 1102 64-65 5.553346669843201 1102 66-67 5.506148454971189 1102 68-69 5.438234896051725 1102 70-71 5.325148537496315 1102 72-73 5.226909696558639 1102 74-75 5.279249586975595 1102 76-77 5.101914764159876 1102 78-79 5.036675577116917 1102 80-81 4.994090384257657 1102 82-83 4.8546455997352815 1102 84-85 4.856080744806029 1102 86-87 4.991222012558687 1102 88-89 5.016157729436792 1102 90-91 4.979895704111467 1102 92-93 5.027446825713032 1102 94-95 5.035064303236673 1102 96-97 5.075571393250115 1102 98-99 4.871693051873148 1102 100-101 4.917922447800027 1103 1 4.68178490497349 1103 2 5.436624366682036 1103 3 5.116173658641024 1103 4 6.351693307873937 1103 5 6.0578621073116 1103 6 5.445768932112035 1103 7 5.730163590048338 1103 8 5.178165802684699 1103 9 5.696361825163606 1103 10-11 6.038253227219361 1103 12-13 6.047614535564538 1103 14-15 6.118160113611399 1103 16-17 6.031502800694753 1103 18-19 5.926404037348451 1103 20-21 5.248283791806234 1103 22-23 5.568646021857386 1103 24-25 5.38549694605641 1103 26-27 5.523325361432505 1103 28-29 5.400982866916753 1103 30-31 5.566168306242226 1103 32-33 5.493205091588685 1103 34-35 5.490046876712668 1103 36-37 5.374418768057986 1103 38-39 5.261659035574919 1103 40-41 5.363254332770111 1103 42-43 5.23905303258671 1103 44-45 5.194363660068191 1103 46-47 5.278896558760437 1103 48-49 4.905489385340413 1103 50-51 4.893793263370682 1103 52-53 4.6426659726531945 1103 54-55 4.919134714329083 1103 56-57 4.962897831467366 1103 58-59 4.829819027200841 1103 60-61 4.791070046633088 1103 62-63 4.728660465558626 1103 64-65 4.703217751875336 1103 66-67 4.802627674561968 1103 68-69 4.75421293707543 1103 70-71 4.661249419580908 1103 72-73 4.41144739526688 1103 74-75 4.378746511707789 1103 76-77 4.402659397328119 1103 78-79 4.4875299067689305 1103 80-81 4.408226504938039 1103 82-83 4.251886790849763 1103 84-85 4.275061510767749 1103 86-87 4.242472681408653 1103 88-89 4.257824673200464 1103 90-91 4.157342660110384 1103 92-93 4.289201747210317 1103 94-95 4.256933627342789 1103 96-97 4.084867050551921 1103 98-99 3.9212178927148464 1103 100-101 3.980699976001997 1104 1 4.140732293373851 1104 2 5.2118839374582 1104 3 4.889104391123311 1104 4 6.109336954740641 1104 5 5.853736662886426 1104 6 5.319244958230652 1104 7 5.845308015067527 1104 8 5.219161931110701 1104 9 6.052720452774828 1104 10-11 5.971307430171027 1104 12-13 5.9308817554194455 1104 14-15 5.951223240654752 1104 16-17 5.743611977299942 1104 18-19 5.988064807482576 1104 20-21 5.3776218098437205 1104 22-23 5.593584820653003 1104 24-25 5.330868185357179 1104 26-27 5.622276431020687 1104 28-29 5.402401522780384 1104 30-31 5.564601512121435 1104 32-33 5.572109587740876 1104 34-35 5.3893901984244295 1104 36-37 5.432673565360808 1104 38-39 5.1100381766094465 1104 40-41 5.37594377512842 1104 42-43 5.271408067918038 1104 44-45 5.365656855023715 1104 46-47 5.3063957477253325 1104 48-49 5.045215520616818 1104 50-51 4.955677427162065 1104 52-53 4.805953374940838 1104 54-55 5.0455295719211435 1104 56-57 4.920492953585487 1104 58-59 4.737995534745906 1104 60-61 4.60848587775531 1104 62-63 4.705998433980827 1104 64-65 4.866035224148007 1104 66-67 4.776259647517975 1104 68-69 4.727974151191447 1104 70-71 4.524542407750577 1104 72-73 4.382288883002875 1104 74-75 4.5691552442645005 1104 76-77 4.47987722350096 1104 78-79 4.6105694057810815 1104 80-81 4.4560381170294185 1104 82-83 4.278501354251482 1104 84-85 4.369324518567922 1104 86-87 4.366422237980359 1104 88-89 4.49109514385556 1104 90-91 4.360933241763867 1104 92-93 4.350024093935087 1104 94-95 4.1691912418947865 1104 96-97 4.130809265226773 1104 98-99 3.986208714303192 1104 100-101 4.141915232502619 1105 1 4.042030850237541 1105 2 4.966523470119089 1105 3 4.75340604927521 1105 4 6.003971169045144 1105 5 3.197817516569195 1105 6 4.447478454241324 1105 7 5.062526718368321 1105 8 0.7197071593931561 1105 9 0.15074269026704457 1105 10-11 1.7837146300971263 1105 12-13 5.1270939185269775 1105 14-15 3.263431944284502 1105 16-17 5.3990829538110745 1105 18-19 5.9086278933025405 1105 20-21 3.0924318232231265 1105 22-23 4.910175668659935 1105 24-25 5.186720103657368 1105 26-27 5.293543801865447 1105 28-29 2.8048264366422586 1105 30-31 5.0034578292176946 1105 32-33 5.31434941628244 1105 34-35 5.3100522268569605 1105 36-37 5.274000066510695 1105 38-39 5.237475752375911 1105 40-41 5.389199946463833 1105 42-43 2.743235444783881 1105 44-45 4.82368736438314 1105 46-47 5.089960692322052 1105 48-49 5.045186545657199 1105 50-51 5.123628769191548 1105 52-53 4.975130373039288 1105 54-55 5.067261351789263 1105 56-57 5.094777284813375 1105 58-59 4.93184137553558 1105 60-61 4.941565871151667 1105 62-63 4.780431438832835 1105 64-65 4.915320612019855 1105 66-67 4.949333141153616 1105 68-69 5.021960711495055 1105 70-71 4.7332683925249235 1105 72-73 4.755895984383557 1105 74-75 4.746914177285291 1105 76-77 4.670592699276419 1105 78-79 4.821041526523047 1105 80-81 4.702133091956657 1105 82-83 4.532893861377843 1105 84-85 4.649755431723193 1105 86-87 4.6094750458372715 1105 88-89 4.521747901319468 1105 90-91 4.558721408431094 1105 92-93 4.643562637200407 1105 94-95 4.625235718916116 1105 96-97 4.438056070673824 1105 98-99 4.291442498433941 1105 100-101 4.453891067080757 1106 1 3.4846895075401036 1106 2 3.8997010632726585 1106 3 4.229256302836756 1106 4 5.5815377819779 1106 5 5.520219382337004 1106 6 4.609180201377757 1106 7 5.206100684133649 1106 8 4.675345252740403 1106 9 5.457185366786035 1106 10-11 5.429689733422986 1106 12-13 5.622673265240319 1106 14-15 5.175787961618131 1106 16-17 5.279974462719537 1106 18-19 5.522146534779186 1106 20-21 4.761138901500939 1106 22-23 4.862171237129342 1106 24-25 5.012353045739584 1106 26-27 5.043279225611663 1106 28-29 5.1220000374795625 1106 30-31 5.142614404889365 1106 32-33 5.053900471913934 1106 34-35 4.600217066192791 1106 36-37 4.663373873513095 1106 38-39 4.718465477668875 1106 40-41 4.805811510967935 1106 42-43 1.9507115459025606 1106 44-45 4.162228295757963 1106 46-47 4.23105268493876 1106 48-49 4.115415325256212 1106 50-51 4.278482677185771 1106 52-53 4.192765022973678 1106 54-55 4.243008625849498 1106 56-57 4.121672636045723 1106 58-59 4.075384598587874 1106 60-61 3.979792516965958 1106 62-63 3.9819273666542117 1106 64-65 4.072422802744441 1106 66-67 4.2233107684733895 1106 68-69 4.205222546552214 1106 70-71 4.024240042018608 1106 72-73 3.76564417236683 1106 74-75 3.805581750318126 1106 76-77 3.717380875800732 1106 78-79 3.927474631009229 1106 80-81 3.7697221774118965 1106 82-83 3.8744221028248127 1106 84-85 3.9481798705427344 1106 86-87 4.013286936156888 1106 88-89 4.046449127375499 1106 90-91 4.08597387892582 1106 92-93 4.150344353747553 1106 94-95 4.049276715154058 1106 96-97 4.1514366619117276 1106 98-99 4.114548984894494 1106 100-101 4.205255854617693 1107 1 2.9056508234431107 1107 2 -0.10683544659866939 1107 3 1.137202532695209 1107 4 2.613582649778131 1107 5 2.7775948351580197 1107 6 3.823906317423205 1107 7 4.8290191455351525 1107 8 4.11531923051194 1107 9 4.9145148013504 1107 10-11 2.482107266926601 1107 12-13 4.884030119015936 1107 14-15 3.6287537423877048 1107 16-17 1.9209652248284357 1107 18-19 2.786502655823636 1107 20-21 2.077035469990758 1107 22-23 4.194606438356498 1107 24-25 4.113795498950932 1107 26-27 3.3344979561377954 1107 28-29 3.946011295517348 1107 30-31 3.327672578265883 1107 32-33 4.197763814126773 1107 34-35 4.73968141916378 1107 36-37 4.824272325190499 1107 38-39 4.890276830208446 1107 40-41 3.9889117170913764 1107 42-43 4.29838262493481 1107 44-45 4.673896799959181 1107 46-47 4.978569274109404 1107 48-49 2.7714702184623583 1107 50-51 4.264961527277187 1107 52-53 4.381679416689991 1107 54-55 4.937420831644232 1107 56-57 3.3803481046170347 1107 58-59 2.4017675773112757 1107 60-61 3.235378325264307 1107 62-63 4.290516893392052 1107 64-65 4.5053246236156355 1107 66-67 4.67869332947155 1107 68-69 4.497149218651593 1107 70-71 4.1113649281713585 1107 72-73 4.008499358665443 1107 74-75 3.9481208350204824 1107 76-77 3.8974221004889813 1107 78-79 3.9537700928695436 1107 80-81 4.123884569813406 1107 82-83 3.931870828762815 1107 84-85 3.8025761890822025 1107 86-87 4.024837792493642 1107 88-89 4.1005931672579905 1107 90-91 4.3954157021253195 1107 92-93 4.052694845773111 1107 94-95 4.196194793070088 1107 96-97 4.137553939986628 1107 98-99 3.9814969198937042 1107 100-101 4.204770060700046 1108 1 -1.8288905827014617 1108 2 -1.0783378531480814 1108 3 -1.6318236019815942 1108 4 -2.9857705057254478 1108 5 -8.093156368116684 1108 6 -6.403303884547018 1108 7 -5.3331301055106834 1108 8 -5.650455588434578 1108 9 -7.40630027669377 1108 10-11 -3.228723105076458 1108 12-13 -1.756545878465058 1108 14-15 -6.2322437386073055 1108 16-17 -5.89380851454451 1108 18-19 -3.7320801789566254 1108 20-21 -4.270906143072079 1108 22-23 -3.296982783900713 1108 24-25 -7.813735201422421 1108 26-27 -8.233051636773006 1108 28-29 -5.403480855083167 1108 30-31 -5.177935131688685 1108 32-33 -2.243566085562847 1108 34-35 -0.8293723795857169 1108 36-37 -4.191573115860287 1108 38-39 -5.447743726671593 1108 40-41 -3.0177022453934406 1108 42-43 -3.5878444903546516 1108 44-45 -1.4672140362605148 1108 46-47 -2.0553428306092343 1108 48-49 -1.1395351344232516 1108 50-51 -0.5197379914129314 1108 52-53 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56-57 0.8180786010709369 2313 58-59 2.7753845985878733 2313 60-61 2.4916903820018987 2313 62-63 -3.881681688168232 2313 64-65 -5.561200199079398 2313 66-67 -5.373363408117104 2313 68-69 -4.163418157234933 2313 70-71 -3.21870599381446 2313 72-73 -2.3617134072920045 2313 74-75 -1.8083330656887426 2313 76-77 -1.122861587454281 2313 78-79 -2.812896573797122 2313 80-81 -2.2379594018178004 2313 82-83 -1.2321008024428615 2313 84-85 -0.6552961513433289 2313 86-87 0.02313566483406504 2313 88-89 -3.3511798823874024 2313 90-91 -2.677066567378226 2313 92-93 -4.550567565574408 2313 94-95 -4.652032157284523 2313 96-97 -4.830389322424718 2313 98-99 -5.9026165552836005 2313 100-101 -3.9373575988744207 2314 1 -14.400181466919843 2314 2 -16.33822468314435 2314 3 -15.959643524876755 2314 4 -25.2510982012857 2314 5 -20.33339390076188 2314 6 -18.35937278145039 2314 7 -18.607101004652584 2314 8 -14.923804674369162 2314 9 -17.528113484006298 2314 10-11 -16.11567746398704 2314 12-13 -17.67864822891898 2314 14-15 -18.01841772194271 2314 16-17 -18.413753173169063 2314 18-19 -19.741920372962348 2314 20-21 -20.735680424877827 2314 22-23 -20.68909819243199 2314 24-25 -20.518544926580983 2314 26-27 -19.28788001632779 2314 28-29 -21.119996213869385 2314 30-31 -21.87559400496315 2314 32-33 -18.5651523310547 2314 34-35 -20.260055752300552 2314 36-37 -20.31814319420875 2314 38-39 -19.041062343759016 2314 40-41 -19.88673283860324 2314 42-43 -20.832543532762312 2314 44-45 -17.89761759303206 2314 46-47 -16.33882747182301 2314 48-49 -15.899981596306102 2314 50-51 -15.5868782724725 2314 52-53 -16.549960132746804 2314 54-55 -17.561853260971535 2314 56-57 -15.989764536183966 2314 58-59 -14.689321283765068 2314 60-61 -14.535760598390258 2314 62-63 -16.809132668560387 2314 64-65 -15.419043336334301 2314 66-67 -15.587088898313183 2314 68-69 -18.49479070625454 2314 70-71 -19.302039327147792 2314 72-73 -17.923478113174355 2314 74-75 -16.28284286961031 2314 76-77 -16.144430214905263 2314 78-79 -15.557994613012811 2314 80-81 -15.378155480249173 2314 82-83 -15.116414527933056 2314 84-85 -14.611178504284506 2314 86-87 -15.718040805754168 2314 88-89 -15.016866156897205 2314 90-91 -14.323144998750776 2314 92-93 -14.03684207537833 2314 94-95 -13.771640000421778 2314 96-97 -14.328428538110995 2314 98-99 -14.647714594499286 2314 100-101 -13.563828187109713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52494.0 3 9675.0 4 6710.0 5 5542.0 6 5835.0 7 6666.0 8 7798.0 9 8812.0 10 9981.0 11 10830.0 12 11780.0 13 12634.0 14 13841.0 15 15842.0 16 17156.0 17 18052.0 18 16356.0 19 14868.0 20 15388.0 21 15900.0 22 18358.0 23 21863.0 24 26336.0 25 32223.0 26 38533.0 27 43824.0 28 49902.0 29 55561.0 30 63848.0 31 72428.0 32 83092.0 33 99888.0 34 121397.0 35 146662.0 36 179856.0 37 212669.0 38 187416.0 39 48431.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33046362011342 20.372151519958063 14.28956317587596 23.007821684052562 2 14.833233156996691 20.47348951077708 41.413404551479466 23.279872780746764 3 18.03524338062064 26.11293850401183 30.731969254817287 25.119848860550242 4 11.876918702846872 17.347555850400692 39.80567298590497 30.969852460847463 5 12.546224846443074 38.02622229960406 35.54096945539904 13.886583398553823 6 29.762081650479754 37.950318109980955 18.449759785315162 13.837840454224132 7 25.96024760391929 33.198164754436554 22.89976004012278 17.94182760152138 8 24.97658316915283 35.78637770897833 20.724852237545736 18.512186884323107 9 25.501132515580533 16.35488715371591 21.64421899203862 36.49976133866494 10-11 24.08646317313462 26.605402076665268 29.10061457853692 20.20752017166319 12-13 24.861273730456816 24.51071992358721 28.984568198470505 21.64343814748547 14-15 22.15964941714023 25.95150322985269 26.777263458470184 25.111583894536892 16-17 21.308261953008472 28.900383175194634 27.54855977862365 22.242795093173243 18-19 21.04075225932273 28.13141117309126 29.766872574873492 21.060963992712516 20-21 22.077684148850576 27.260088889789436 29.643700703750703 21.018526257609285 22-23 21.951818058757958 27.11385933673916 29.35936305255672 21.57495955194616 24-25 21.691513643676423 27.32888876506908 29.381849310731717 21.597748280522776 26-27 21.600562525351503 27.39369540366908 29.364420102624845 21.64132196835457 28-29 21.344764161763486 27.6513841256528 29.494942956094416 21.508908756489298 30-31 21.863082924198284 27.276051517658562 29.37521622626648 21.485649331876672 32-33 21.82914556459738 27.576825790996928 29.05514323891388 21.538885405491808 34-35 21.937185408265 27.668164477857616 29.26467221923924 21.129977894638145 36-37 21.873054795812504 27.2534526274597 29.3495926948301 21.52389988189769 38-39 21.86359162843701 27.446416546741524 29.327636607683765 21.362355217137697 40-41 21.859956950704955 27.098906430826176 29.423247519937316 21.61788909853155 42-43 21.6770768278621 27.348970574701127 29.20653210056911 21.767420496867665 44-45 21.78840197018608 27.6230972351662 28.9946524485505 21.59384834609722 46-47 21.841745365315564 27.497495525714477 28.775143233417005 21.885615875552954 48-49 21.903249763118136 27.563143404778273 28.819327871800404 21.714278960303186 50-51 21.751222457769 27.670146593635288 28.58879779037487 21.989833158220844 52-53 21.583046796229556 28.015547997110566 28.344140558936594 22.05726464772328 54-55 21.62239679175603 27.823817319610306 28.36901574087 22.184770147763665 56-57 21.764566172641846 27.79568008945138 28.196563997285317 22.243189740621457 58-59 21.82125121273528 27.883361878514606 28.417148775077415 21.8782381336727 60-61 21.976387431974825 27.86257682532754 28.25479608287631 21.90623965982132 62-63 21.97316262672267 27.751828798587564 28.146320755247427 22.128687819442337 64-65 21.56014371742321 27.83974045398062 28.151578024054114 22.448537804542052 66-67 21.852048648010392 27.622927314744533 28.188660043070247 22.336363994174835 68-69 21.60206129919201 27.60440036210493 28.23853381239141 22.55500452631165 70-71 21.68651496494217 27.52503528273985 28.181238016519632 22.607211735798348 72-73 21.88179298168671 27.595010373968933 28.237409262914042 22.285787381430314 74-75 21.73560154962918 27.74277617529477 28.25155047259192 22.27007180248413 76-77 21.800965988001057 27.746740212202486 27.931278781438184 22.52101501835827 78-79 21.65752970216307 27.72554245455414 27.97693549021923 22.639992353063555 80-81 21.72534003564822 27.785171690591458 28.080787634453948 22.408700639306378 82-83 21.80895028929035 27.6243048878543 28.401987078926506 22.16475774392884 84-85 22.06768101029525 27.666897199309524 27.88562207265714 22.37979971773808 86-87 21.54099826257106 27.88460997812751 28.121580666745384 22.452811092556043 88-89 21.75067051262588 27.57136672120732 28.04570169076014 22.632261075406664 90-91 21.80431152269622 27.431051060169022 28.251184994011773 22.513452423122988 92-93 21.970829186557136 27.463831677434232 28.117026241362055 22.448312894646584 94-95 21.51482437348537 27.530573688915876 28.265382431163516 22.689219506435236 96-97 21.418647279433678 27.536308891250442 28.100888956361857 22.944154872954023 98-99 21.33155092241171 27.626553986809032 27.96473412838981 23.077160962389442 100-101 21.560340513581746 27.429026871109762 27.900184988389025 23.110447626919463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 296.0 1 193.5 2 114.5 3 136.5 4 133.0 5 135.5 6 194.5 7 354.0 8 633.5 9 903.0 10 991.5 11 996.5 12 1092.0 13 1278.0 14 1486.5 15 1668.5 16 1851.0 17 1992.5 18 2195.0 19 2489.5 20 2801.5 21 3211.0 22 3733.0 23 4534.5 24 5777.0 25 7395.5 26 9664.5 27 12442.0 28 16116.0 29 20861.5 30 25660.0 31 30648.5 32 36072.5 33 40669.0 34 44672.5 35 48648.5 36 52488.5 37 57191.5 38 59887.5 39 59729.5 40 59950.0 41 60071.5 42 60082.5 43 61080.0 44 62074.0 45 64327.0 46 66308.5 47 67364.5 48 67615.5 49 65768.5 50 63405.0 51 61665.0 52 59140.5 53 55510.0 54 52055.5 55 47344.0 56 42783.5 57 38335.0 58 33729.0 59 29443.5 60 23770.5 61 18180.0 62 13849.0 63 10516.5 64 8005.0 65 5919.5 66 4411.0 67 3379.5 68 2637.0 69 2042.0 70 1565.0 71 1196.0 72 926.0 73 729.5 74 542.5 75 372.5 76 284.0 77 203.0 78 151.5 79 122.5 80 83.5 81 69.0 82 58.5 83 40.0 84 36.5 85 35.0 86 27.0 87 21.0 88 19.0 89 13.5 90 8.5 91 6.5 92 6.5 93 6.0 94 3.5 95 2.5 96 3.5 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034411214007388294 2 0.18481970660504135 3 0.0 4 0.32656916822697907 5 0.21034697974124114 6 0.09817316937401953 7 0.10924998172607099 8 0.11189267299788021 9 0.10761938498389083 10-11 0.11178021805014367 12-13 0.16142907747583624 14-15 0.12639936125589685 16-17 0.12080472760600285 18-19 0.12898582505383782 20-21 0.10095642933049948 22-23 0.10281193596815275 24-25 0.09640200394716866 26-27 0.12451574088130943 28-29 0.11287665379057514 30-31 0.12839543657822083 32-33 0.10165927275385299 34-35 0.1504928338084555 36-37 0.13986584124735027 38-39 0.13393384275424658 40-41 0.10806920477483709 42-43 0.10910941304140029 44-45 0.090076413136987 46-47 0.09389988136003014 48-49 0.09898846774510962 50-51 0.08642162733554869 52-53 0.093871767623096 54-55 0.31248418602297456 56-57 0.33213568813993893 58-59 0.3459957604484703 60-61 0.1660116165961012 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1778490.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.546152709014066 #Duplication Level Percentage of deduplicated Percentage of total 1 80.16719961088862 49.33982709505764 2 11.544962850143706 14.210960931896777 3 3.681835233164183 6.798083805292538 4 1.6168765061467194 3.980501134356925 5 0.8449487525206221 2.600167247696257 6 0.4850761786681991 1.7912743540690785 7 0.32356443823163356 1.393990242862733 8 0.22495118047358692 1.107590376440029 9 0.1642958622106807 0.910060040256992 >10 0.8390723159831297 9.412346008860359 >50 0.06791409865668063 2.937463426936711 >100 0.0371355497690771 4.00101649727782 >500 0.0013193010437264811 0.5106188160499429 >1k 8.481220995384521E-4 1.0061000229462016 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4769 0.2681488228778346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3653 0.2053989620408324 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2001 0.11251117521043132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.622747386828152E-5 0.0 4 0.0 0.0 0.0 5.622747386828152E-5 0.0 5 0.0 0.0 0.0 1.1245494773656303E-4 0.0 6 0.0 0.0 0.0 1.1245494773656303E-4 0.0 7 0.0 0.0 0.0 1.1245494773656303E-4 0.0 8 0.0 0.0 0.0 1.1245494773656303E-4 0.0 9 0.0 0.0 0.0 1.1245494773656303E-4 0.0 10-11 0.0 0.0 0.0 1.1245494773656303E-4 0.0 12-13 0.0 0.0 0.0 1.1245494773656303E-4 0.0 14-15 0.0 0.0 0.0 1.1245494773656303E-4 0.0 16-17 0.0 0.0 0.0 1.6868242160484456E-4 0.0 18-19 0.0 0.0 0.0 2.2490989547312607E-4 0.0 20-21 0.0 0.0 0.0 2.2490989547312607E-4 0.0 22-23 0.0 0.0 0.0 3.0925110627554834E-4 0.0 24-25 0.0 0.0 0.0 4.7793352788039293E-4 0.0 26-27 0.0 0.0 0.0 9.277533188266451E-4 0.0 28-29 0.0 0.0 0.0 0.003036283588887202 0.0 30-31 0.0 0.0 0.0 0.009361874399068873 0.0 32-33 0.0 0.0 0.0 0.024318382448031757 0.0 34-35 0.0 0.0 0.0 0.049705086899560866 0.0 36-37 5.622747386828152E-5 0.0 0.0 0.08895186365962136 0.0 38-39 1.1245494773656303E-4 0.0 0.0 0.1426491012038302 0.0 40-41 1.1245494773656303E-4 0.0 0.0 0.1989890300198483 0.0 42-43 1.1245494773656303E-4 0.0 0.0 0.2557506648898785 0.0 44-45 1.1245494773656303E-4 0.0 0.0 0.3274969215458057 0.0 46-47 1.1245494773656303E-4 0.0 0.0 0.4121192697175694 0.0 48-49 1.1245494773656303E-4 0.0 0.0 0.5025330476977661 0.0 50-51 1.1245494773656303E-4 2.811373693414076E-5 0.0 0.5943806262616039 0.0 52-53 1.1245494773656303E-4 5.622747386828152E-5 0.0 0.6857783850344956 0.0 54-55 1.1245494773656303E-4 5.622747386828152E-5 0.0 0.7852447863074856 0.0 56-57 1.1245494773656303E-4 5.622747386828152E-5 0.0 0.9021417044796429 0.0 58-59 1.1245494773656303E-4 5.622747386828152E-5 0.0 1.0370876417635184 0.0 60-61 1.1245494773656303E-4 5.622747386828152E-5 0.0 1.1787527621746539 0.0 62-63 1.1245494773656303E-4 5.622747386828152E-5 0.0 1.3180282149463871 0.0 64-65 1.1245494773656303E-4 5.622747386828152E-5 0.0 1.4619143205753196 0.0 66-67 1.1245494773656303E-4 5.622747386828152E-5 0.0 1.6298376712829423 0.0 68-69 1.1245494773656303E-4 5.622747386828152E-5 0.0 1.8194085994298534 0.0 70-71 1.1245494773656303E-4 5.622747386828152E-5 0.0 2.0261570208435247 0.0 72-73 1.1245494773656303E-4 5.622747386828152E-5 0.0 2.231106163093411 0.0 74-75 1.1245494773656303E-4 5.622747386828152E-5 0.0 2.432119382172517 0.0 76-77 1.1245494773656303E-4 5.622747386828152E-5 0.0 2.663411095929693 0.0 78-79 1.1245494773656303E-4 5.622747386828152E-5 0.0 2.9180934388160744 0.0 80-81 1.1245494773656303E-4 5.622747386828152E-5 0.0 3.1939173118769295 0.0 82-83 1.1245494773656303E-4 5.622747386828152E-5 0.0 3.4813521582915845 0.0 84-85 1.1245494773656303E-4 5.622747386828152E-5 0.0 3.762124049052848 0.0 86-87 1.1245494773656303E-4 5.622747386828152E-5 0.0 4.055322211539002 0.0 88-89 1.1245494773656303E-4 5.622747386828152E-5 0.0 4.375678243903536 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2760 0.0 47.523125 1 GTATCAA 5175 0.0 41.290398 1 ATCAACG 5965 0.0 35.209454 3 CAACGCA 6010 0.0 35.04791 5 TATCAAC 6255 0.0 34.084866 2 TCAACGC 6255 0.0 33.809273 4 AACGCAG 6320 0.0 33.606045 6 ACGCAGA 7105 0.0 29.500954 7 CGCAGAG 7125 0.0 29.484852 8 GCAGAGT 7765 0.0 25.830484 9 GTACATG 5440 0.0 25.338467 1 ACATGGG 5395 0.0 23.481256 3 TACATGG 5695 0.0 23.282652 2 CAACGCG 105 0.0021620838 22.680931 4 GAGTACT 4305 0.0 22.57526 12-13 GTACTAG 750 0.0 22.258104 1 GTGGTAT 1420 0.0 21.832647 1 CTGTGCG 760 0.0 21.263113 9 GCATCGT 540 0.0 21.12414 8 AGAGTAC 6880 0.0 21.10495 10-11 >>END_MODULE