FastQCFastQC Report
Sat 18 Jun 2016
SRR1931148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778490
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55940.3145364888191668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47100.2648314019196059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.17149379529825864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31500.052.068881
GTATCAA59350.040.9326481
TATCAAC72150.033.6332
ATCAACG70600.033.496983
CAACGCA70400.033.389785
TCAACGC73700.032.2168854
AACGCAG76200.031.0352616
GTGGTAT13500.028.1725351
ACGCAGA85350.027.2629977
CGCAGAG85300.026.8892738
GTACTAG7100.026.7837471
GCAGAGT93150.024.0624669
GAGTACT52850.023.76630412-13
GTACATG54500.022.5057181
TGGTATC17100.021.6610492
TACATGG56950.021.0963752
GTACTTT60800.021.01017614-15
TAGGCAT9800.020.8364355
AGAGTAC83300.020.63690610-11
ACATGGG55850.020.4915523
Produced by FastQC (version 0.11.5)