FastQCFastQC Report
Sat 18 Jun 2016
SRR1931147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401524
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44880.18688133035522442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.1308752275638303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.043.8850751
GTATCAA57750.040.184421
ATCAACG63250.034.8103263
CAACGCA64700.034.3599435
TCAACGC66500.033.6216854
TATCAAC69500.032.906732
AACGCAG69550.032.554716
ACGCAGA77200.028.2255847
CGCAGAG78650.027.5243138
GTACATG67850.026.7672981
TACATGG68850.024.7036932
GCAGAGT87500.023.6529339
ACATGGG70800.023.6103553
GTACTAG7950.023.5700261
GAGTACT48000.020.76657112-13
CATGGGG49850.020.0297894
AGAGTAC78750.019.96754810-11
CAGAGTA86500.019.74613810-11
GTACTTT52700.019.04676614-15
GCATCGT8000.019.0310968
Produced by FastQC (version 0.11.5)