FastQCFastQC Report
Sat 18 Jun 2016
SRR1931147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401524
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60650.25254796537532004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46420.19329392502427625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29780.12400459041841765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.046.9870451
GTATCAA64700.038.8822671
ATCAACG78000.031.5991763
CAACGCA79750.030.904495
TCAACGC81750.030.0909524
TATCAAC84550.029.5443952
AACGCAG84950.029.0127496
ACGCAGA96150.024.9417537
CGCAGAG96400.024.3351948
GTACTAG7600.022.5262621
GTACATG70400.021.9538611
GTGGTAT20100.021.7666681
GCAGAGT107200.021.396239
TACATGG71300.020.9808962
GAGTACT64700.020.73476212-13
ACATGGG69900.020.3820133
AGAGTAC97350.019.78061510-11
TGGTATC21700.019.0398042
CAGAGTA107300.018.80936410-11
GTACTTT70800.018.7806114-15
Produced by FastQC (version 0.11.5)