##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931147_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2401524 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.304841842096934 31.0 31.0 34.0 30.0 34.0 2 31.33601204901554 31.0 31.0 34.0 30.0 34.0 3 31.492926991360488 31.0 31.0 34.0 30.0 34.0 4 35.0892982955823 35.0 35.0 37.0 32.0 37.0 5 34.98641570935789 35.0 35.0 37.0 32.0 37.0 6 35.10695375103476 37.0 35.0 37.0 32.0 37.0 7 34.960153219372366 36.0 35.0 37.0 32.0 37.0 8 35.01228261720474 36.0 35.0 37.0 32.0 37.0 9 36.412067920204 38.0 35.0 39.0 32.0 39.0 10-11 36.288565719101705 38.0 35.0 39.0 32.0 39.0 12-13 36.20823318859191 38.0 35.0 39.0 32.0 39.0 14-15 37.159706919439486 39.0 36.0 41.0 31.5 41.0 16-17 37.206105581289215 39.0 36.0 41.0 31.5 41.0 18-19 37.27322150434474 39.0 36.0 41.0 31.5 41.0 20-21 37.2549558113931 39.0 36.0 40.5 31.0 41.0 22-23 37.14067171512757 39.0 36.0 40.0 31.0 41.0 24-25 36.99767064580658 39.0 36.0 40.0 31.0 41.0 26-27 36.75538595491862 39.0 35.5 40.0 30.5 41.0 28-29 36.47811035825585 38.0 35.0 40.0 30.0 41.0 30-31 36.26683889063778 38.0 35.0 40.0 30.0 41.0 32-33 36.12722213061373 38.0 35.0 40.0 30.0 41.0 34-35 35.90055793737643 38.0 34.5 40.0 29.0 41.0 36-37 36.14648198394019 38.0 35.0 40.0 30.0 41.0 38-39 36.13681562207998 38.0 35.0 40.0 30.0 41.0 40-41 35.97675996575508 38.0 35.0 40.0 29.0 41.0 42-43 35.79943589987025 38.0 34.5 40.0 28.5 41.0 44-45 35.5614503540252 38.0 34.0 40.0 27.5 41.0 46-47 35.364137939075356 38.0 34.0 40.0 27.0 41.0 48-49 35.09737233523379 38.0 34.0 40.0 26.5 41.0 50-51 34.75748628787387 38.0 33.0 40.0 26.0 41.0 52-53 34.639587195464216 38.0 33.0 40.0 26.0 41.0 54-55 34.39787880529197 38.0 33.0 40.0 24.5 41.0 56-57 34.160102501578166 37.0 33.0 40.0 24.0 41.0 58-59 33.89725253630611 37.0 32.5 40.0 24.0 41.0 60-61 33.60186448272014 37.0 32.0 40.0 23.0 41.0 62-63 33.3308413740608 36.0 32.0 39.0 22.5 41.0 64-65 33.07968793982488 36.0 31.0 39.0 22.0 41.0 66-67 32.829286319853566 35.5 31.0 39.0 22.0 40.0 68-69 32.49002237745698 35.0 31.0 39.0 21.5 40.0 70-71 31.67078842435054 35.0 30.0 38.0 18.5 40.0 72-73 31.635680717744233 35.0 30.0 37.0 20.0 40.0 74-75 31.32539795563151 34.5 30.0 37.0 18.5 39.0 76-77 30.599705853449727 34.0 29.5 36.0 18.0 39.0 78-79 30.619265932799337 34.0 30.0 36.0 18.0 39.0 80-81 30.358270206752046 34.0 30.0 36.0 16.0 37.5 82-83 30.012601164926938 34.0 29.0 35.0 12.0 37.0 84-85 29.66179913255083 34.0 29.0 35.0 8.5 36.5 86-87 29.345640518270898 34.0 29.0 35.0 7.0 36.0 88-89 29.0452371077699 34.0 29.0 35.0 3.5 36.0 90-91 28.74100696058003 34.0 29.0 35.0 2.0 35.5 92-93 28.403282873708527 33.5 29.0 35.0 2.0 35.0 94-95 28.029254756562914 33.0 27.5 35.0 2.0 35.0 96-97 27.669710983525462 33.0 27.0 35.0 2.0 35.0 98-99 27.20048477550089 33.0 27.0 35.0 2.0 35.0 100-101 26.524093242457706 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8075837520947218 1101 2 0.6319723213280852 1101 3 0.5189655193770228 1101 4 0.7695291813684975 1101 5 0.7502697060934906 1101 6 0.7720127899491516 1101 7 0.7688152221734654 1101 8 0.7631629238597384 1101 9 1.1874028119955824 1101 10-11 0.9966234349588845 1101 12-13 1.0677313103562938 1101 14-15 1.4504265415063244 1101 16-17 1.202692993864062 1101 18-19 1.010849095777047 1101 20-21 0.7992275654886285 1101 22-23 0.8329429900480108 1101 24-25 0.7517402891305593 1101 26-27 1.0357200210468847 1101 28-29 0.7634401039786312 1101 30-31 0.9772341980847301 1101 32-33 1.0625080896944112 1101 34-35 0.7676909436293684 1101 36-37 1.239878768274906 1101 38-39 1.0785662356619525 1101 40-41 1.079271422380799 1101 42-43 1.08459170593094 1101 44-45 1.0833782310605287 1101 46-47 1.1575039833825613 1101 48-49 0.9950440248513175 1101 50-51 0.8240793554124721 1101 52-53 0.7052502636265245 1101 54-55 0.85208424188977 1101 56-57 0.6192784195678698 1101 58-59 0.7625260424840405 1101 60-61 0.9024929313016159 1101 62-63 0.6984957394735432 1101 64-65 0.7284374519837726 1101 66-67 0.45435496115107554 1101 68-69 0.41475294683587904 1101 70-71 0.8488848469906713 1101 72-73 0.34627514020670347 1101 74-75 -0.06982934816885589 1101 76-77 -0.07037686409032062 1101 78-79 0.10351851455146033 1101 80-81 0.0688573987666885 1101 82-83 0.07984449161037332 1101 84-85 0.2162657889868953 1101 86-87 -0.009759358467857027 1101 88-89 -0.1686489377385456 1101 90-91 -0.06855489583041319 1101 92-93 -0.24384571106640962 1101 94-95 -0.21010993769706943 1101 96-97 -0.2656951934411609 1101 98-99 -0.367090049660181 1101 100-101 -0.6327498606624751 1102 1 0.9587203833583366 1102 2 0.8134749816162774 1102 3 0.6170125883380422 1102 4 0.9096723597478515 1102 5 0.8850613953068347 1102 6 0.7930435698692193 1102 7 0.9019775086985717 1102 8 0.9473855568836811 1102 9 1.3328760107028828 1102 10-11 1.1961051032987697 1102 12-13 1.3178815775582677 1102 14-15 1.718379872006473 1102 16-17 1.4866157670513545 1102 18-19 1.2919717986920318 1102 20-21 1.2700106472230175 1102 22-23 0.9265306411254244 1102 24-25 0.7098750577085156 1102 26-27 0.8996275020864104 1102 28-29 1.0375812991300748 1102 30-31 1.4470543789684882 1102 32-33 1.4503824195631054 1102 34-35 1.2639351581147835 1102 36-37 1.5874336230625588 1102 38-39 1.3964839167458862 1102 40-41 1.3251841456882545 1102 42-43 1.4784853759378862 1102 44-45 1.5249268264500628 1102 46-47 1.466667511111531 1102 48-49 1.3128561508147385 1102 50-51 1.1077243828394927 1102 52-53 1.222907209778164 1102 54-55 1.0409617120159709 1102 56-57 1.0647469842909913 1102 58-59 1.2281995700868933 1102 60-61 1.4557189908342352 1102 62-63 1.3230760632834517 1102 64-65 1.286596094378929 1102 66-67 1.1111118526627664 1102 68-69 0.994965394479923 1102 70-71 1.376455821004285 1102 72-73 0.7784501534707502 1102 74-75 0.7170111926229588 1102 76-77 0.7908655774562199 1102 78-79 0.7964998469274924 1102 80-81 0.6607119984212488 1102 82-83 0.7131571186599928 1102 84-85 0.7732554942097778 1102 86-87 0.7927982781767504 1102 88-89 0.6443285056102646 1102 90-91 0.7675815873216827 1102 92-93 0.7052547240881673 1102 94-95 0.6900313988202207 1102 96-97 0.7524671502755247 1102 98-99 0.8369267347478129 1102 100-101 0.8593706944888275 1103 1 0.6740317339247603 1103 2 0.5180873556378955 1103 3 0.3390090841063582 1103 4 0.6676167655354632 1103 5 0.5742253123252894 1103 6 0.543038627944398 1103 7 0.5408099562839368 1103 8 0.5405593284490777 1103 9 0.8430544572128795 1103 10-11 0.8259686550101364 1103 12-13 0.8714299202808746 1103 14-15 1.3427431195278388 1103 16-17 1.0962994047917576 1103 18-19 0.9343227514021137 1103 20-21 0.858958782361519 1103 22-23 0.7045178079350407 1103 24-25 0.5317360807822951 1103 26-27 0.7767415703161333 1103 28-29 0.5992025958260285 1103 30-31 1.0273669074572354 1103 32-33 1.0903377015388713 1103 34-35 0.8132569923061155 1103 36-37 1.1553604088532552 1103 38-39 0.9851699263776368 1103 40-41 1.0430588726614332 1103 42-43 1.283024196676017 1103 44-45 1.2322472589952227 1103 46-47 1.213804334288767 1103 48-49 1.1243316025880645 1103 50-51 0.938734856464265 1103 52-53 0.5650311711599869 1103 54-55 0.6747126108648871 1103 56-57 0.6569805982636439 1103 58-59 0.9024061992895085 1103 60-61 1.1468581079948237 1103 62-63 0.7725852894501202 1103 64-65 0.7336505792745456 1103 66-67 0.6115526432870126 1103 68-69 0.48695947626331915 1103 70-71 0.9761667707282768 1103 72-73 0.49206831338167234 1103 74-75 0.42783539172880936 1103 76-77 0.44500464880226787 1103 78-79 0.4764372193676927 1103 80-81 0.30816606991613327 1103 82-83 0.3657768388237912 1103 84-85 0.4486307142126442 1103 86-87 0.32305599781217254 1103 88-89 0.10961956521476424 1103 90-91 0.19555524232742272 1103 92-93 0.004489551332426345 1103 94-95 -0.027286227874451896 1103 96-97 -0.05784619334011154 1103 98-99 -0.12607691134618193 1103 100-101 -0.1568952731609805 1104 1 0.4896561354131741 1104 2 0.40769531344795595 1104 3 0.1808963450437311 1104 4 0.6632971079068852 1104 5 0.3373319750702777 1104 6 0.5228230155701397 1104 7 0.5575527194238319 1104 8 0.5401229280003648 1104 9 0.7568803598823592 1104 10-11 0.6379192783742056 1104 12-13 0.7720461877118083 1104 14-15 1.22031728936377 1104 16-17 0.9294871573066459 1104 18-19 0.7118536288999024 1104 20-21 0.6318362892700549 1104 22-23 0.4421990144431973 1104 24-25 0.43646224178077375 1104 26-27 0.1906902818631906 1104 28-29 0.5816873252303907 1104 30-31 0.8958346664930446 1104 32-33 0.9514638324407656 1104 34-35 0.8991829923334791 1104 36-37 1.0192256707847278 1104 38-39 0.7022704569439071 1104 40-41 0.9141928583154026 1104 42-43 1.0382477493039985 1104 44-45 1.1097902492275225 1104 46-47 1.198120936658185 1104 48-49 1.0904052297734879 1104 50-51 0.8287716335762738 1104 52-53 0.5773420129978888 1104 54-55 0.9298991127755585 1104 56-57 0.9511517509171554 1104 58-59 0.9414813107836721 1104 60-61 1.1681964711055386 1104 62-63 0.2897805843510355 1104 64-65 -0.6633084688526267 1104 66-67 0.0035085050525012207 1104 68-69 0.5443579914985754 1104 70-71 1.1900129087895124 1104 72-73 0.6876710487506301 1104 74-75 0.5733642821681286 1104 76-77 0.2603724598230883 1104 78-79 0.29308715621046844 1104 80-81 0.30514826049134314 1104 82-83 0.14251588549173277 1104 84-85 -0.040676876282304875 1104 86-87 0.3193656323666936 1104 88-89 0.3097749502230158 1104 90-91 0.44467440427439797 1104 92-93 0.46347543329910224 1104 94-95 0.44990110397229444 1104 96-97 0.4811038007317734 1104 98-99 0.40905908188664597 1104 100-101 0.09236217346318298 1105 1 -0.3469312544556402 1105 2 -1.7396891130701455 1105 3 -0.36021033121965473 1105 4 0.28666608966100426 1105 5 0.48106909419180965 1105 6 -0.10519303573976657 1105 7 -0.16587002750215163 1105 8 -0.2167911242352858 1105 9 0.5706199405001655 1105 10-11 0.9628606057888689 1105 12-13 1.0042940046485 1105 14-15 1.2291774642581004 1105 16-17 0.790551236874947 1105 18-19 0.542959636993416 1105 20-21 0.9319125229522527 1105 22-23 0.3241777112488222 1105 24-25 -0.1514105442141016 1105 26-27 0.8398967695503501 1105 28-29 0.3616175101334207 1105 30-31 0.7725066404365037 1105 32-33 0.9239398726652794 1105 34-35 0.5071274975363735 1105 36-37 0.7037151630344738 1105 38-39 0.4922982465791037 1105 40-41 0.9517824683776936 1105 42-43 1.196669545905472 1105 44-45 1.3297305501532293 1105 46-47 1.3605920504281315 1105 48-49 1.1908520380852465 1105 50-51 0.8040409354633908 1105 52-53 0.8319710627391501 1105 54-55 0.19830963357762954 1105 56-57 0.4885097172157771 1105 58-59 -0.2914406307033488 1105 60-61 0.46360181653378163 1105 62-63 0.9860253311683067 1105 64-65 1.4377632687040958 1105 66-67 1.1007459861282243 1105 68-69 1.035089402095192 1105 70-71 0.5224080693008801 1105 72-73 -0.15297417485605536 1105 74-75 -0.05099231005472049 1105 76-77 0.16452231405473228 1105 78-79 -0.36628458408124587 1105 80-81 -0.27011283640817396 1105 82-83 -0.23314659801607007 1105 84-85 -0.17253206552151568 1105 86-87 -0.34719018041695193 1105 88-89 -0.6943767042590707 1105 90-91 -0.8784524437951973 1105 92-93 -0.35191920063900994 1105 94-95 -0.6660254347311891 1105 96-97 -0.8971167001740668 1105 98-99 -1.6035492144290373 1105 100-101 -0.9671561141385929 1106 1 0.2744954905556334 1106 2 0.3751496373043466 1106 3 -0.23661720972208045 1106 4 0.37171213686422533 1106 5 0.06526555038971793 1106 6 0.30682598724055055 1106 7 0.3178408626107583 1106 8 0.43391754102508884 1106 9 0.8917206009572212 1106 10-11 0.16039500382628802 1106 12-13 0.32303770427621714 1106 14-15 0.09645175744994816 1106 16-17 -0.20687290081051657 1106 18-19 0.3422244042679381 1106 20-21 0.4239301968394571 1106 22-23 0.3957244242502185 1106 24-25 0.24950459061539476 1106 26-27 0.584120839949982 1106 28-29 0.4713127087125599 1106 30-31 0.15851966816366314 1106 32-33 0.254708496596443 1106 34-35 -1.2721043497174307 1106 36-37 -1.0162548146556105 1106 38-39 -0.7669547825109646 1106 40-41 0.30796215898567425 1106 42-43 0.5982291067549355 1106 44-45 0.45598153089327553 1106 46-47 0.025484572676653272 1106 48-49 -0.005131094720773888 1106 50-51 0.6412139530485632 1106 52-53 -0.05222506347580236 1106 54-55 0.6148417106837485 1106 56-57 0.20696584053301592 1106 58-59 0.5473897838411972 1106 60-61 0.7995985983558072 1106 62-63 0.6613686055324095 1106 64-65 0.7912956902203305 1106 66-67 0.5712407902012302 1106 68-69 0.6422643750212451 1106 70-71 0.9825065300761935 1106 72-73 0.33008806852463835 1106 74-75 0.4877618861006532 1106 76-77 0.44138192917488794 1106 78-79 0.4286494638365781 1106 80-81 0.48191813195520794 1106 82-83 0.38161410755195746 1106 84-85 0.38912168167286154 1106 86-87 0.5050218488278269 1106 88-89 0.4087274300743857 1106 90-91 0.7481807032538015 1106 92-93 0.3498497612560918 1106 94-95 0.4102916039925404 1106 96-97 0.5906246631743137 1106 98-99 0.543390354794326 1106 100-101 0.3917860326353697 1107 1 0.28815120431497476 1107 2 0.3507127448244347 1107 3 0.13036538932343333 1107 4 -1.3299692901280906 1107 5 -0.016862612223889073 1107 6 0.22701648974958744 1107 7 0.5264745565083615 1107 8 0.40436300458265606 1107 9 0.8729990030697223 1107 10-11 0.8269588087882127 1107 12-13 0.8880832024865484 1107 14-15 0.9748235487788648 1107 16-17 0.9295057092186099 1107 18-19 0.6125756693133866 1107 20-21 0.2581859408651539 1107 22-23 0.5939306499055448 1107 24-25 0.4392932635458777 1107 26-27 0.7138022537830153 1107 28-29 0.5989895804008043 1107 30-31 0.9023308039849809 1107 32-33 1.0822896893535798 1107 34-35 0.9564839117662345 1107 36-37 1.0351691839231876 1107 38-39 0.9706053115340225 1107 40-41 0.3863189816336714 1107 42-43 0.30739443556147705 1107 44-45 0.027896173224043253 1107 46-47 0.3368194829303661 1107 48-49 0.6503130638748686 1107 50-51 -0.9086149214820836 1107 52-53 0.4601859468937164 1107 54-55 0.9794119395674912 1107 56-57 0.8260636293353443 1107 58-59 0.9209888688326018 1107 60-61 1.1336646842595854 1107 62-63 1.209025595741906 1107 64-65 1.0398193835038327 1107 66-67 0.9232826169724646 1107 68-69 0.9004537787304052 1107 70-71 1.2967049544717355 1107 72-73 0.9056834711108763 1107 74-75 0.7020588954349698 1107 76-77 0.7611828073375015 1107 78-79 0.6979299473085199 1107 80-81 0.8573125265406034 1107 82-83 0.9255060084538087 1107 84-85 0.90640787627229 1107 86-87 0.9175660969065724 1107 88-89 0.944671838696344 1107 90-91 1.1419428207574 1107 92-93 1.1627541349620962 1107 94-95 0.933376544951237 1107 96-97 0.8997210154219175 1107 98-99 0.8628566582648673 1107 100-101 0.8041263118752902 1108 1 0.46080696175761204 1108 2 0.45056875282941533 1108 3 0.251177520445637 1108 4 0.5762845994861792 1108 5 0.5039369094298252 1108 6 0.4648526236051964 1108 7 0.535613573864687 1108 8 0.5268831085497538 1108 9 0.7207453656448237 1108 10-11 0.7176424453589263 1108 12-13 0.8998466163171059 1108 14-15 1.0867935951898389 1108 16-17 0.821912978780567 1108 18-19 0.5925990171679416 1108 20-21 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1.198324163579226 2311 82-83 1.4441681059764733 2311 84-85 1.2603701808342933 2311 86-87 1.170243329718737 2311 88-89 1.3197842824744548 2311 90-91 1.6852782958769836 2311 92-93 1.6915968564584922 2311 94-95 1.8449386210560448 2311 96-97 1.867337207325967 2311 98-99 2.0762285437506947 2311 100-101 2.158859659487195 2312 1 -0.058356777152642536 2312 2 -0.1703054571029874 2312 3 -0.41345411553558264 2312 4 0.15569239275777846 2312 5 -1.1903743905366895 2312 6 -0.13464043190591468 2312 7 0.2598567834963106 2312 8 -2.385715629901867 2312 9 -4.985680126420906 2312 10-11 -0.5985781784185065 2312 12-13 -5.74782429746287 2312 14-15 -2.1077131551818624 2312 16-17 -3.153332280813828 2312 18-19 -2.506944700794719 2312 20-21 -3.605574250932854 2312 22-23 -1.9153638652379925 2312 24-25 -3.032316045805956 2312 26-27 -3.4261946102791327 2312 28-29 -6.171664279500327 2312 30-31 -6.276046061560841 2312 32-33 -1.5838932673300192 2312 34-35 0.43148863253288283 2312 36-37 0.8178107563431212 2312 38-39 0.8368794801732946 2312 40-41 -2.823373932495585 2312 42-43 -4.7581318287609875 2312 44-45 -3.737492683069071 2312 46-47 0.16189686978370332 2312 48-49 -1.4003035019697094 2312 50-51 -7.033019936285065 2312 52-53 -1.5230484189387283 2312 54-55 0.7302771886553714 2312 56-57 1.1552932676571785 2312 58-59 1.3357436250050014 2312 60-61 1.4647165385092862 2312 62-63 1.1535513610118997 2312 64-65 1.3713310067057236 2312 66-67 0.8646673864304617 2312 68-69 0.8565730725037106 2312 70-71 1.449550227972722 2312 72-73 0.876704707816863 2312 74-75 0.8938471218429385 2312 76-77 0.9977097393112544 2312 78-79 0.775759878317885 2312 80-81 0.8142230545246321 2312 82-83 -0.24581853750959937 2312 84-85 0.07197679609344121 2312 86-87 0.7395232256014133 2312 88-89 0.7265865816506007 2312 90-91 0.9528010898545887 2312 92-93 1.1190120169564146 2312 94-95 1.2205508963516216 2312 96-97 1.1475364806534714 2312 98-99 1.356392411328308 2312 100-101 1.5223875860776452 2313 1 0.14403754685799086 2313 2 0.060272250785740056 2313 3 -0.0566268288531937 2313 4 -0.9509944918129634 2313 5 -0.9361529670464748 2313 6 -0.7543285520152452 2313 7 -1.8023039114005428 2313 8 -4.093445234155119 2313 9 -1.7865712118228316 2313 10-11 -0.12996273044217332 2313 12-13 -3.907929505445196 2313 14-15 -2.827400339277112 2313 16-17 -5.603199366814806 2313 18-19 -3.316259912895511 2313 20-21 -2.7926600647034974 2313 22-23 -2.2298645953289764 2313 24-25 -0.5881680058194405 2313 26-27 -2.07983664105528 2313 28-29 -4.139348393469401 2313 30-31 -2.3573108037825676 2313 32-33 -6.7597749925911685 2313 34-35 -2.9018119403077307 2313 36-37 -3.0774352281564816 2313 38-39 -2.355499493206466 2313 40-41 -1.035395161294872 2313 42-43 -1.806252312493065 2313 44-45 -3.36547856797678 2313 46-47 -2.0476954024840737 2313 48-49 -0.3560908770118303 2313 50-51 1.6280774466195709 2313 52-53 0.4557274618505147 2313 54-55 -2.4232010396571795 2313 56-57 -5.141863121574914 2313 58-59 -0.29677724810893125 2313 60-61 -6.500828783292356 2313 62-63 -0.8460040323012166 2313 64-65 -1.3816127575684831 2313 66-67 -1.8168865841412583 2313 68-69 -2.7593663112246176 2313 70-71 -1.9775436355189768 2313 72-73 1.4227285200149922 2313 74-75 2.649360244760306 2313 76-77 -0.6015358333421581 2313 78-79 1.3058152903512834 2313 80-81 2.42396134498852 2313 82-83 2.778251064497219 2313 84-85 2.7888559379557307 2313 86-87 2.4439814606532693 2313 88-89 -1.2221947280202983 2313 90-91 1.7907255722388022 2313 92-93 2.773430939417011 2313 94-95 2.6861430188571447 2313 96-97 2.981155298280491 2313 98-99 2.916058628708214 2313 100-101 3.146240968832526 2314 1 -28.809985441647758 2314 2 -14.794827758264852 2314 3 -5.212025507466642 2314 4 -3.9073253814862383 2314 5 -2.0246225118016596 2314 6 -1.908595906526962 2314 7 -0.1382619166498813 2314 8 -4.3976356586279195 2314 9 -2.896988444151546 2314 10-11 -0.8058610017789434 2314 12-13 -0.18598634316806795 2314 14-15 -1.4387905103333125 2314 16-17 -1.1130645129818788 2314 18-19 -3.4906195834539275 2314 20-21 -2.139388275401295 2314 22-23 -4.372954467715623 2314 24-25 -0.8708858988414363 2314 26-27 -3.5074771515087093 2314 28-29 -2.4955344736576492 2314 30-31 -4.434693372340806 2314 32-33 -3.059530626585193 2314 34-35 -2.4181708868187215 2314 36-37 -5.154614158376411 2314 38-39 -8.286873373195604 2314 40-41 -7.254306374979365 2314 42-43 -5.02186005980051 2314 44-45 -6.029520562727438 2314 46-47 -7.728662005796586 2314 48-49 -5.904692560422092 2314 50-51 -4.088933142574017 2314 52-53 -4.997005817179257 2314 54-55 -5.955602161930692 2314 56-57 -2.985310492377703 2314 58-59 -3.0865998564897765 2314 60-61 -1.5873750770745971 2314 62-63 -0.6742237806947387 2314 64-65 -2.4212996070571293 2314 66-67 -2.2141849821863318 2314 68-69 -0.8342147137949851 2314 70-71 -0.10443294676886694 2314 72-73 -0.4695467990184028 2314 74-75 -0.29024967468941654 2314 76-77 -2.7196913225266997 2314 78-79 -3.862548362467976 2314 80-81 -3.7278849759455 2314 82-83 -3.4873385816246056 2314 84-85 -1.4911467772221094 2314 86-87 -0.9245134257617913 2314 88-89 -0.7268105303553511 2314 90-91 -0.05088905351918527 2314 92-93 -0.08685053401949716 2314 94-95 0.31615478462780544 2314 96-97 0.4930794543127064 2314 98-99 1.0020392604395951 2314 100-101 1.540982574407689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 21.0 4 57.0 5 89.0 6 229.0 7 655.0 8 1519.0 9 2701.0 10 4025.0 11 5841.0 12 7612.0 13 9668.0 14 11644.0 15 14289.0 16 16455.0 17 18778.0 18 17100.0 19 14693.0 20 14466.0 21 15206.0 22 16841.0 23 19435.0 24 23169.0 25 28257.0 26 35992.0 27 45304.0 28 57573.0 29 71966.0 30 88866.0 31 106824.0 32 129387.0 33 156879.0 34 193517.0 35 237105.0 36 296849.0 37 362719.0 38 309386.0 39 66366.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.85622971223427 20.94608483908427 14.827596990374452 23.370088458307013 2 15.096163935900703 21.070037193049078 40.38677106703909 23.44702780401112 3 18.492267075313773 26.101348368881744 30.33296965961806 25.073414896186424 4 12.73374818239812 17.28062607119836 38.57121917988471 31.414406566518814 5 12.44023561803096 38.326155916642584 35.85521466874646 13.37839379657999 6 30.51503961651018 38.53698734636839 17.591745908015078 13.356227129106351 7 26.72865230578583 32.7544925638886 22.556884711541507 17.95997041878407 8 25.186506568329108 36.42466200629267 20.004172350557393 18.38465907482082 9 25.30805438546523 16.185472225137037 20.999873413715626 37.50659997568211 10-11 24.241835601059993 26.87368520989172 28.763276985780696 20.12120220326759 12-13 25.010576617181425 24.317745731460523 28.760945133173767 21.910732518184286 14-15 22.20893899040776 25.986102991267213 26.3663407069844 25.438617311340632 16-17 21.58231606263356 29.008183969845813 27.070976596527874 22.338523370992753 18-19 21.502762412534707 28.039778074256184 29.137747530318247 21.319711982890865 20-21 22.258074456053738 27.15673464017016 29.117635301583494 21.467555602192608 22-23 22.22116039648157 27.062169688914206 28.811517186586517 21.90515272801771 24-25 22.068135255929953 27.228008957975234 28.9165631329423 21.787292653152512 26-27 21.974689131901354 27.327408863384612 28.801460851024846 21.89644115368919 28-29 21.804938512724405 27.28139852970235 29.01088366733636 21.90277929023689 30-31 22.19684407862903 27.17622323808826 28.753309453356174 21.873623229926537 32-33 22.125323965807866 27.34442145869561 28.55898093182227 21.971273643674255 34-35 22.254073614471377 27.24676546890811 28.78530708786015 21.713853828760364 36-37 22.179105310431353 27.028487837297067 28.723608473271284 22.06879837900029 38-39 22.08855559677984 27.21136024737903 28.815776159620437 21.884307996220688 40-41 22.17928751379375 26.972558246059673 28.94213912427908 21.906015115867497 42-43 22.08831612065166 27.085806205393308 28.617135590181952 22.208742083773082 44-45 22.203583424040456 27.25685841610792 28.467454951109765 22.07210320874186 46-47 22.274387856268067 27.19286589074307 28.24411183963875 22.288634413350106 48-49 22.269882283951233 27.274501131180255 28.172219279043343 22.28339730582516 50-51 22.27289122320525 27.405393612705325 27.978769445488037 22.34294571860139 52-53 22.265827267804173 27.479152964181885 27.852630908308434 22.402388859705503 54-55 22.228100192549814 27.399557000055193 27.920659897049894 22.4516829103451 56-57 22.4028218965145 27.349864491253506 27.800040432970068 22.447273179261927 58-59 22.236562865073058 27.42386648947101 27.959221957548415 22.38034868790752 60-61 22.31777189146287 27.470270801485952 27.83996765256658 22.371989654484604 62-63 22.295569585595608 27.417154205121978 27.77712654665368 22.510149662628734 64-65 22.111378953424378 27.35054534263584 27.793849010645744 22.74422669329404 66-67 22.243469273280063 27.317116540121322 27.734210218378692 22.705203968219926 68-69 22.177057849863104 27.33470054862116 27.73206050448162 22.756181097034116 70-71 22.25424998912088 27.17334158966781 27.737931617478807 22.834476803732507 72-73 22.35758697067102 27.202255505404526 27.70745806739615 22.7326994565283 74-75 22.218624934988576 27.432384995883822 27.560117609778217 22.78887245934938 76-77 22.168443871474945 27.38538111632447 27.464872306085635 22.98130270611495 78-79 22.22061907355496 27.409303425658038 27.31711196723414 23.05296553355286 80-81 22.11383471364142 27.524518877026477 27.4026584030011 22.958988006330998 82-83 22.233432742844723 27.380061078136542 27.591740043988917 22.794766135029818 84-85 22.252309629541106 27.424884599200965 27.308458408554742 23.014347362703187 86-87 21.97519703417091 27.50039871129846 27.458112411185237 23.066291843345393 88-89 22.19852571299125 27.262129429440435 27.34670396663324 23.192640890935078 90-91 22.139116617308545 27.317430468341403 27.36254817830219 23.180904736047864 92-93 22.154531637135896 27.32776031147848 27.351891565512503 23.16581648587312 94-95 21.919546284517416 27.228508789280053 27.43136075086248 23.420584175340046 96-97 21.96834081819185 27.18698404965199 27.3987253879322 23.44594974422396 98-99 21.786153077717298 27.339084093784688 27.271813063571855 23.602949764926162 100-101 21.85155712238736 27.26657946931207 27.239762326828508 23.64210108147206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 527.0 1 346.0 2 199.0 3 229.5 4 192.0 5 167.5 6 286.0 7 566.5 8 933.5 9 1171.0 10 1111.5 11 1036.5 12 1078.5 13 1184.5 14 1388.5 15 1563.0 16 1760.5 17 1971.5 18 2172.5 19 2410.0 20 2729.0 21 3152.5 22 3818.5 23 4846.5 24 6193.5 25 8131.0 26 10852.5 27 14173.5 28 18303.0 29 23663.5 30 29623.0 31 35883.0 32 43069.0 33 50101.0 34 56641.5 35 62677.0 36 69450.5 37 76340.5 38 79446.5 39 80099.5 40 80316.0 41 80301.0 42 80765.5 43 81958.5 44 83614.0 45 87602.5 46 90004.5 47 89458.0 48 89150.5 49 87727.0 50 86313.5 51 84502.5 52 81119.0 53 77411.5 54 74194.5 55 68703.0 56 62469.5 57 56651.0 58 50062.5 59 43885.0 60 36796.5 61 29656.5 62 23504.5 63 18259.0 64 14030.5 65 10373.5 66 7693.5 67 5986.0 68 4648.0 69 3553.5 70 2772.0 71 2137.5 72 1615.5 73 1255.5 74 986.0 75 766.5 76 561.0 77 394.0 78 294.0 79 218.0 80 148.5 81 100.5 82 77.0 83 64.5 84 56.5 85 48.0 86 34.5 87 18.5 88 11.5 89 10.0 90 7.0 91 6.0 92 3.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1340398846732325 2 0.0 3 0.004330583412866164 4 3.3312180098970487E-4 5 4.164022512371311E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.022277520441186513 26-27 0.007661801422763213 28-29 0.007224579058964224 30-31 0.0029148157586599173 32-33 0.0014990481044536718 34-35 0.003914181161629032 36-37 0.0017905296803196636 38-39 0.001124286078340254 40-41 0.0030813766591547696 42-43 0.0020195509185000856 44-45 9.160849527216884E-4 46-47 0.03930837251678517 48-49 0.02048699076086685 50-51 0.08176058203041069 52-53 0.0018113497928815203 54-55 0.03524845056722315 56-57 7.703441647886924E-4 58-59 0.0014782279918918154 60-61 0.004039101837000171 62-63 0.003726800148572323 64-65 0.009806273016634438 66-67 0.006037832642938401 68-69 0.002040371031061942 70-71 0.005642250504263126 72-73 0.004622064988732155 74-75 9.577251778454016E-4 76-77 0.0 78-79 0.0 80-81 2.914815758659918E-4 82-83 4.164022512371311E-4 84-85 1.0410056280928278E-4 86-87 0.0015406883295773848 88-89 0.0038309007113816063 90-91 0.0011659263034639671 92-93 0.0030813766591547696 94-95 7.91164277350549E-4 96-97 3.7476202611341796E-4 98-99 0.0018529900180052333 100-101 0.003123016884278483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2401524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.87332841171777 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3156331946756 46.890176260364385 2 12.805757606794355 15.334466615049028 3 4.054876170566972 7.283367978876147 4 1.6766239360874735 4.015402221932489 5 0.868007524868252 2.598524980013957 6 0.5113581798035368 1.837002974123723 7 0.33116212461951294 1.3879445051406416 8 0.22446741028985476 1.0751688779209818 9 0.17167136476799108 0.9250682401477552 >10 0.9222481685359138 10.208019730150726 >50 0.07728712019270412 3.174636897779268 >100 0.03915025073442234 3.959836008523468 >500 0.0011244467605226825 0.4312546686973878 >1k 5.622233802602759E-4 0.6239286005888346 >5k 7.027792253253449E-5 0.25520144069134626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6065 0.25254796537532004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4642 0.19329392502427625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2978 0.12400459041841765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.164022512371311E-5 0.0 0.0 0.0 3 0.0 4.164022512371311E-5 0.0 0.0 0.0 4 0.0 8.328045024742622E-5 0.0 0.0 0.0 5 0.0 8.328045024742622E-5 0.0 0.0 0.0 6 0.0 8.328045024742622E-5 0.0 0.0 0.0 7 0.0 8.328045024742622E-5 0.0 0.0 0.0 8 0.0 8.328045024742622E-5 0.0 0.0 0.0 9 0.0 1.2492067537113932E-4 0.0 0.0 0.0 10-11 0.0 1.2492067537113932E-4 0.0 4.164022512371311E-5 0.0 12-13 0.0 1.2492067537113932E-4 0.0 4.164022512371311E-5 0.0 14-15 0.0 1.2492067537113932E-4 0.0 4.164022512371311E-5 4.164022512371311E-5 16-17 0.0 1.2492067537113932E-4 0.0 4.164022512371311E-5 4.164022512371311E-5 18-19 0.0 1.6656090049485244E-4 0.0 4.164022512371311E-5 4.164022512371311E-5 20-21 0.0 2.4984135074227864E-4 0.0 6.246033768556966E-5 4.164022512371311E-5 22-23 0.0 2.914815758659918E-4 0.0 1.87381013056709E-4 4.164022512371311E-5 24-25 8.328045024742622E-5 2.914815758659918E-4 0.0 6.454234894175531E-4 4.164022512371311E-5 26-27 1.6656090049485244E-4 2.914815758659918E-4 0.0 0.0012283866411495368 4.164022512371311E-5 28-29 1.6656090049485244E-4 2.914815758659918E-4 0.0 0.003539419135515614 6.246033768556966E-5 30-31 2.0820112561856555E-4 3.7476202611341796E-4 0.0 0.010597437293984987 8.328045024742622E-5 32-33 2.0820112561856555E-4 3.7476202611341796E-4 0.0 0.025962680364635123 8.328045024742622E-5 34-35 2.290212381804221E-4 4.164022512371311E-4 0.0 0.052924726132239364 8.328045024742622E-5 36-37 2.4984135074227864E-4 4.580424763608442E-4 0.0 0.09398198810422048 8.328045024742622E-5 38-39 2.4984135074227864E-4 4.996827014845573E-4 0.0 0.1501338316835476 8.328045024742622E-5 40-41 2.4984135074227864E-4 6.246033768556967E-4 0.0 0.21373927556001937 8.328045024742622E-5 42-43 2.914815758659918E-4 6.454234894175531E-4 0.0 0.27836490495202215 8.328045024742622E-5 44-45 3.1230168842784833E-4 8.119843899124056E-4 0.0 0.3562321259333656 1.2492067537113932E-4 46-47 3.3312180098970487E-4 9.577251778454015E-4 0.0 0.45148414090385935 1.2492067537113932E-4 48-49 3.3312180098970487E-4 9.577251778454015E-4 0.0 0.5490263682561574 1.2492067537113932E-4 50-51 3.3312180098970487E-4 9.993654029691146E-4 0.0 0.6491502895661255 1.2492067537113932E-4 52-53 3.3312180098970487E-4 9.993654029691146E-4 0.0 0.7479833638972586 1.2492067537113932E-4 54-55 3.3312180098970487E-4 9.993654029691146E-4 0.0 0.8610365751081397 1.2492067537113932E-4 56-57 3.3312180098970487E-4 0.0010410056280928278 0.0 0.9878102404972843 1.2492067537113932E-4 58-59 3.3312180098970487E-4 0.0010410056280928278 0.0 1.1259308672326407 1.2492067537113932E-4 60-61 3.3312180098970487E-4 0.0010618257406546843 0.0 1.2700893266109354 1.2492067537113932E-4 62-63 3.3312180098970487E-4 0.0011034659657783974 0.0 1.4138730239631168 1.2492067537113932E-4 64-65 3.3312180098970487E-4 0.0011659263034639671 0.0 1.5689828625489481 1.2492067537113932E-4 66-67 3.3312180098970487E-4 0.0011659263034639671 0.0 1.7474112272040587 1.2492067537113932E-4 68-69 3.3312180098970487E-4 0.0012075665285876803 0.0 1.9423915813458454 1.4574078793299586E-4 70-71 3.3312180098970487E-4 0.0012492067537113933 0.0 2.1503636857262305 1.6656090049485244E-4 72-73 3.3312180098970487E-4 0.0012492067537113933 0.0 2.366705475356482 1.6656090049485244E-4 74-75 3.3312180098970487E-4 0.0012700268662732498 0.0 2.5894390395432234 1.6656090049485244E-4 76-77 3.3312180098970487E-4 0.0013533073165206762 0.0 2.829120175355316 1.6656090049485244E-4 78-79 4.164022512371311E-4 0.0013741274290825327 0.0 3.096138118961126 1.6656090049485244E-4 80-81 4.164022512371311E-4 0.0013741274290825327 0.0 3.385204561769943 1.87381013056709E-4 82-83 4.580424763608442E-4 0.0013741274290825327 0.0 3.687616696730909 2.0820112561856555E-4 84-85 4.996827014845573E-4 0.0013741274290825327 0.0 3.9797436960863184 2.0820112561856555E-4 86-87 4.996827014845573E-4 0.0014157676542062457 0.0 4.286403134009904 2.0820112561856555E-4 88-89 4.996827014845573E-4 0.0014157676542062457 0.0 4.62693689507163 2.290212381804221E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3765 0.0 46.987045 1 GTATCAA 6470 0.0 38.882267 1 ATCAACG 7800 0.0 31.599176 3 CAACGCA 7975 0.0 30.90449 5 TCAACGC 8175 0.0 30.090952 4 TATCAAC 8455 0.0 29.544395 2 AACGCAG 8495 0.0 29.012749 6 ACGCAGA 9615 0.0 24.941753 7 CGCAGAG 9640 0.0 24.335194 8 GTACTAG 760 0.0 22.526262 1 GTACATG 7040 0.0 21.953861 1 GTGGTAT 2010 0.0 21.766668 1 GCAGAGT 10720 0.0 21.39623 9 TACATGG 7130 0.0 20.980896 2 GAGTACT 6470 0.0 20.734762 12-13 ACATGGG 6990 0.0 20.382013 3 AGAGTAC 9735 0.0 19.780615 10-11 TGGTATC 2170 0.0 19.039804 2 CAGAGTA 10730 0.0 18.809364 10-11 GTACTTT 7080 0.0 18.78061 14-15 >>END_MODULE