FastQCFastQC Report
Sat 18 Jun 2016
SRR1931146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830023
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55920.1975955672445065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40800.1441684396204554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.054.8532261
GTATCAA65250.044.5855141
ATCAACG77800.036.205833
CAACGCA79050.035.6578185
TCAACGC80600.035.3710564
AACGCAG82350.034.5487676
TATCAAC85450.033.426862
ACGCAGA88350.031.2448967
CGCAGAG88850.030.6397868
GCAGAGT100000.025.6501169
GTACATG69750.022.9261551
TACATGG69800.021.875442
TATACCG2657.548806E-1021.5975555
CAGAGTA100300.021.31832710-11
GAGTACT65400.021.22271212-13
ACATGGG68550.021.034873
AGAGTAC94350.020.94470410-11
GTACTTT73900.019.03477914-15
GTGGTAT19700.017.8481831
GTGTAGC19300.017.4694041
Produced by FastQC (version 0.11.5)