##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931146_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2830023 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.777579899527318 31.0 28.0 33.0 23.0 34.0 2 28.457190277252163 31.0 28.0 33.0 16.0 34.0 3 28.563785170650558 31.0 28.0 34.0 16.0 34.0 4 31.763226305934616 35.0 32.0 37.0 19.0 37.0 5 31.849770478897167 35.0 32.0 37.0 19.0 37.0 6 32.14756593851004 35.0 32.0 37.0 22.0 37.0 7 31.84926730277457 35.0 32.0 37.0 18.0 37.0 8 32.05255858344614 35.0 32.0 37.0 21.0 37.0 9 33.13122614197835 37.0 32.0 39.0 18.0 39.0 10-11 33.09951862582035 37.0 32.0 39.0 20.0 39.0 12-13 33.11099432760794 37.0 32.0 39.0 19.0 39.0 14-15 33.88757123175324 38.0 32.0 40.0 18.0 41.0 16-17 33.909274412257425 38.0 32.0 40.0 19.0 41.0 18-19 33.93152423142851 38.0 32.0 40.0 18.5 41.0 20-21 34.18973397035996 38.0 32.0 40.0 23.0 41.0 22-23 34.0351922934902 38.0 32.0 40.0 21.5 41.0 24-25 34.00308425055202 38.0 32.0 40.0 21.0 41.0 26-27 33.64990796894584 38.0 32.0 40.0 18.5 41.0 28-29 33.597496734125485 38.0 32.0 40.0 18.0 41.0 30-31 33.35727713166995 37.5 31.5 40.0 17.5 41.0 32-33 33.15282419966198 37.0 31.0 40.0 17.0 41.0 34-35 33.10203591984941 37.0 31.0 40.0 17.5 41.0 36-37 32.772376231571265 37.0 30.5 40.0 15.5 41.0 38-39 32.59608296469675 37.0 30.0 40.0 15.0 41.0 40-41 32.48646830785474 37.0 30.0 40.0 14.0 41.0 42-43 32.74453140486844 37.0 30.0 40.0 15.0 41.0 44-45 32.75947845653552 37.0 30.5 40.0 14.0 41.0 46-47 32.57581422483139 37.0 30.0 40.0 10.5 41.0 48-49 32.37684782067142 37.0 30.0 40.0 9.0 41.0 50-51 31.85682872542025 36.5 30.0 39.5 8.0 40.5 52-53 31.859395312334918 36.5 30.0 39.5 8.0 40.5 54-55 31.836989134010572 36.5 30.0 40.0 7.0 41.0 56-57 31.622968258561855 36.0 30.0 40.0 4.0 41.0 58-59 31.474183778718405 36.0 30.0 40.0 2.0 41.0 60-61 31.3240171546309 36.0 29.0 40.0 2.0 41.0 62-63 31.177122765433356 35.5 29.5 39.5 2.0 41.0 64-65 30.88260166083456 35.0 29.0 39.0 2.0 41.0 66-67 30.646217716251776 35.0 29.0 39.0 2.0 40.0 68-69 30.495632544329144 35.0 29.0 39.0 2.0 40.0 70-71 30.196057063847185 35.0 29.0 38.5 2.0 40.0 72-73 29.855638629085348 34.0 29.0 38.0 2.0 40.0 74-75 29.450282912895055 34.0 28.0 37.0 2.0 39.5 76-77 29.120268810536167 34.0 28.0 36.5 2.0 39.0 78-79 28.71114669386079 34.0 28.0 36.0 2.0 39.0 80-81 28.340045469595125 34.0 27.5 36.0 2.0 38.5 82-83 27.974613987236147 33.0 27.0 35.0 2.0 37.0 84-85 27.626888368045066 33.0 26.0 35.0 2.0 37.0 86-87 27.310476451958166 33.0 26.0 35.0 2.0 36.0 88-89 26.962667264541665 33.0 26.0 35.0 2.0 36.0 90-91 26.583737481992195 33.0 25.5 35.0 2.0 36.0 92-93 26.235031835430313 32.5 25.0 35.0 2.0 35.0 94-95 25.942843574062827 32.0 24.5 35.0 2.0 35.0 96-97 25.55818645290162 32.0 23.0 35.0 2.0 35.0 98-99 25.211014716134816 32.0 20.5 35.0 2.0 35.0 100-101 24.63242895905793 31.5 16.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.1405433949796056 1101 2 5.800342371051325 1101 3 5.369423653530955 1101 4 6.753714896252411 1101 5 6.11609697177914 1101 6 5.6274402058576065 1101 7 6.244268149421867 1101 8 5.706467569163337 1101 9 6.3094574010316435 1101 10-11 6.316677153817736 1101 12-13 6.1500842956529596 1101 14-15 6.344710915192792 1101 16-17 6.36823065904866 1101 18-19 6.453581682272365 1101 20-21 5.789238040550359 1101 22-23 5.834398221860138 1101 24-25 5.642211017732198 1101 26-27 5.887338609244278 1101 28-29 5.866928401480035 1101 30-31 5.952889406116494 1101 32-33 5.735872272703599 1101 34-35 5.597784424627829 1101 36-37 5.8246463046659755 1101 38-39 5.718878996696933 1101 40-41 5.899717437047503 1101 42-43 5.903596315773388 1101 44-45 5.7942341310603 1101 46-47 5.574531040933721 1101 48-49 5.392419454238816 1101 50-51 5.327672488271439 1101 52-53 5.093473804753586 1101 54-55 5.3358942289533005 1101 56-57 5.367643349114935 1101 58-59 5.247296415649156 1101 60-61 5.161239073352824 1101 62-63 5.150108442737814 1101 64-65 5.248943128822443 1101 66-67 5.202929081221328 1101 68-69 5.086924472373273 1101 70-71 5.069912567053766 1101 72-73 4.896740838704904 1101 74-75 4.99306632555081 1101 76-77 4.859380787727861 1101 78-79 4.913986076538258 1101 80-81 4.772080525925389 1101 82-83 4.642110937837199 1101 84-85 4.522599076124358 1101 86-87 4.3801875049346 1101 88-89 4.473123659907227 1101 90-91 4.388402309990109 1101 92-93 4.4645460784706685 1101 94-95 4.39559398161089 1101 96-97 4.245977298222993 1101 98-99 3.9395637611017627 1101 100-101 4.035361202475205 1102 1 5.3300488707944815 1102 2 5.961671127010817 1102 3 5.749906102466397 1102 4 7.120818955858013 1102 5 6.587964352615867 1102 6 6.046614757155179 1102 7 6.546902430764238 1102 8 6.206111857277072 1102 9 6.891939805824272 1102 10-11 6.8572665547239495 1102 12-13 6.903453281047248 1102 14-15 7.064598714102395 1102 16-17 7.0005514160608335 1102 18-19 7.08736363800454 1102 20-21 6.290853465329739 1102 22-23 6.42410018795071 1102 24-25 6.232286997285652 1102 26-27 6.453218598751096 1102 28-29 6.4089475998971 1102 30-31 6.518232900384074 1102 32-33 6.25912176164945 1102 34-35 6.277646064708559 1102 36-37 6.4518684614602435 1102 38-39 6.228751444185097 1102 40-41 6.346257836735408 1102 42-43 6.232216371841499 1102 44-45 6.138962459845402 1102 46-47 6.093768777950714 1102 48-49 5.830937761122687 1102 50-51 5.894022042740723 1102 52-53 5.779578493815485 1102 54-55 5.9540328436204035 1102 56-57 5.946309632985901 1102 58-59 6.020000374400222 1102 60-61 5.9561822066265435 1102 62-63 5.750241717809377 1102 64-65 5.767225982658676 1102 66-67 5.812094468673074 1102 68-69 5.754437286846592 1102 70-71 5.637586848187215 1102 72-73 5.516972593528305 1102 74-75 5.578500507184334 1102 76-77 5.403208999062759 1102 78-79 5.260236152987883 1102 80-81 5.276255454627165 1102 82-83 5.147610593203137 1102 84-85 5.1038047511378934 1102 86-87 5.145584796149624 1102 88-89 5.134876300599384 1102 90-91 5.145431400103956 1102 92-93 5.166154814434474 1102 94-95 5.128980109411749 1102 96-97 5.098541250123503 1102 98-99 4.767911841841329 1102 100-101 4.6740176325582965 1103 1 4.858117960027094 1103 2 5.498232581576914 1103 3 5.194799324574497 1103 4 6.629242294342987 1103 5 6.057844922552359 1103 6 5.486306210030467 1103 7 6.049717018047634 1103 8 5.555936253469561 1103 9 5.991448795116902 1103 10-11 6.131483673497911 1103 12-13 6.193140356308298 1103 14-15 6.319669423093529 1103 16-17 6.236461336394857 1103 18-19 6.201170261990903 1103 20-21 5.39052631602636 1103 22-23 5.747082361529838 1103 24-25 5.37827938015435 1103 26-27 5.572071093615751 1103 28-29 5.4712985248032275 1103 30-31 5.627912147926235 1103 32-33 5.42587725964 1103 34-35 5.3126089061490624 1103 36-37 5.427769447500317 1103 38-39 5.299430831761409 1103 40-41 5.539796143913211 1103 42-43 5.457856475177952 1103 44-45 5.139414641403263 1103 46-47 5.039472064990264 1103 48-49 4.888560615091169 1103 50-51 4.859526298103589 1103 52-53 4.678422102247005 1103 54-55 5.081745881393395 1103 56-57 5.079750516998267 1103 58-59 5.114998832539296 1103 60-61 4.979213800623576 1103 62-63 5.035450176003739 1103 64-65 4.9389181793570565 1103 66-67 5.0702916029159795 1103 68-69 5.099389472654156 1103 70-71 5.030734194391638 1103 72-73 4.843050949274939 1103 74-75 4.886571210481687 1103 76-77 4.952044669200408 1103 78-79 4.753986727008222 1103 80-81 4.831385523407281 1103 82-83 4.837062453185826 1103 84-85 4.743687164540969 1103 86-87 4.653977458327443 1103 88-89 4.631937749677995 1103 90-91 4.630596300830312 1103 92-93 4.661043691935792 1103 94-95 4.52819154471387 1103 96-97 4.423557446471012 1103 98-99 4.450340003376201 1103 100-101 4.472725749496789 1104 1 4.414463649805722 1104 2 5.331562352537738 1104 3 4.911017524165679 1104 4 6.445469827350152 1104 5 5.996098319808905 1104 6 5.544386418984995 1104 7 6.137325020276254 1104 8 5.5353168851352095 1104 9 6.219799723008066 1104 10-11 6.167323456506672 1104 12-13 6.17619806888041 1104 14-15 6.164799552999014 1104 16-17 6.136491189076505 1104 18-19 6.276438205839362 1104 20-21 5.586077589610156 1104 22-23 5.785826903084558 1104 24-25 5.4262504665140945 1104 26-27 5.6545587197148315 1104 28-29 5.5975019832969615 1104 30-31 5.695952750423171 1104 32-33 5.637457250221086 1104 34-35 5.5754252537340285 1104 36-37 5.668961837702646 1104 38-39 5.3963945147986045 1104 40-41 5.742383815681908 1104 42-43 5.667312579267627 1104 44-45 5.55479422288149 1104 46-47 5.600099904646456 1104 48-49 5.3791609389820785 1104 50-51 5.282824677437837 1104 52-53 4.944291581193035 1104 54-55 5.359909464114175 1104 56-57 5.337065136476326 1104 58-59 5.240094215063305 1104 60-61 5.105983854465279 1104 62-63 5.01198660945612 1104 64-65 5.091344083790002 1104 66-67 5.200136162353807 1104 68-69 5.135776792317955 1104 70-71 5.067007696253693 1104 72-73 5.004194691718261 1104 74-75 4.933931884311573 1104 76-77 4.939889319834972 1104 78-79 4.938567496323937 1104 80-81 4.8795717770667295 1104 82-83 4.674019966193828 1104 84-85 4.63591916191336 1104 86-87 4.656273941767044 1104 88-89 4.680971847995199 1104 90-91 4.71238641439162 1104 92-93 4.6664520858828915 1104 94-95 4.508278238611311 1104 96-97 4.4269278071042315 1104 98-99 4.160007178037187 1104 100-101 4.294144549846472 1105 1 4.614931532587029 1105 2 5.413577195017098 1105 3 5.0570635555565495 1105 4 6.579322300631205 1105 5 3.6284143365070776 1105 6 4.941929451217771 1105 7 5.780215789413052 1105 8 0.8924112606280197 1105 9 1.7632134687068408 1105 10-11 2.4391083507434956 1105 12-13 5.70200611355456 1105 14-15 4.457148423392354 1105 16-17 6.056647624720679 1105 18-19 6.444922691031721 1105 20-21 4.093957166575855 1105 22-23 5.619412194549074 1105 24-25 5.649740029849081 1105 26-27 5.873111112979462 1105 28-29 3.7241754760677033 1105 30-31 5.701734589487334 1105 32-33 5.725321364096711 1105 34-35 5.761170789219481 1105 36-37 5.847676977074563 1105 38-39 5.641385661427552 1105 40-41 5.8879489811888455 1105 42-43 3.646533261626349 1105 44-45 5.349708253563968 1105 46-47 5.589561584696767 1105 48-49 5.333584785411926 1105 50-51 5.492917366968882 1105 52-53 5.4323475257812746 1105 54-55 5.752611303018732 1105 56-57 5.710604591143046 1105 58-59 5.690885186804028 1105 60-61 5.415594266238337 1105 62-63 5.327992841345381 1105 64-65 5.390290856445443 1105 66-67 5.579273267907659 1105 68-69 5.530841862830318 1105 70-71 5.300064056730918 1105 72-73 5.278758675854931 1105 74-75 5.390974020464725 1105 76-77 5.120435260835212 1105 78-79 5.14823432167297 1105 80-81 5.182922829419326 1105 82-83 5.159850521051123 1105 84-85 5.085394021500953 1105 86-87 5.0495581480221965 1105 88-89 5.039025745366075 1105 90-91 5.006690515486714 1105 92-93 5.172876450409131 1105 94-95 5.153403912282407 1105 96-97 5.129505797031431 1105 98-99 4.961782087869079 1105 100-101 5.11417471484237 1106 1 4.279384959112242 1106 2 4.677509200352695 1106 3 4.630866885543398 1106 4 6.271172168884327 1106 5 6.069654963500131 1106 6 5.195680297060182 1106 7 5.882782590006169 1106 8 5.44705700606584 1106 9 6.0761573892710885 1106 10-11 5.975732304464088 1106 12-13 6.26081561676591 1106 14-15 5.895146347096528 1106 16-17 5.9379941924279755 1106 18-19 6.19721429524521 1106 20-21 5.569538157202352 1106 22-23 5.486601657213637 1106 24-25 5.584693692103617 1106 26-27 5.754210449919487 1106 28-29 5.89556058535155 1106 30-31 5.957142012244905 1106 32-33 5.788367523452404 1106 34-35 5.376077433030797 1106 36-37 5.455578100862972 1106 38-39 5.414362731637329 1106 40-41 5.560667520377923 1106 42-43 2.463051616658184 1106 44-45 4.7082263309515895 1106 46-47 4.9504342727554445 1106 48-49 4.731375736759137 1106 50-51 4.879434823345541 1106 52-53 4.802588526319788 1106 54-55 5.136880076025818 1106 56-57 5.060985815419883 1106 58-59 5.049544668253173 1106 60-61 4.862082469377334 1106 62-63 4.927682838843911 1106 64-65 5.002090534136723 1106 66-67 5.2399448986381785 1106 68-69 5.156386840186226 1106 70-71 5.117588771424668 1106 72-73 4.832731368809277 1106 74-75 4.790099407674447 1106 76-77 4.694383113351144 1106 78-79 4.70638552644094 1106 80-81 4.664954185778349 1106 82-83 4.758582813688118 1106 84-85 4.929199873087381 1106 86-87 4.874292659130312 1106 88-89 4.986869073555614 1106 90-91 5.015564768814922 1106 92-93 5.007386284312169 1106 94-95 4.961172585029082 1106 96-97 4.989350463814791 1106 98-99 4.8822311874367195 1106 100-101 4.948106492160985 1107 1 2.74413609340926 1107 2 -0.3564655732566173 1107 3 0.5199591198272913 1107 4 2.3972329943900768 1107 5 1.9882235697209545 1107 6 3.6692339511839585 1107 7 4.799475036150493 1107 8 4.263168242168355 1107 9 4.764480457685131 1107 10-11 2.5318096405534902 1107 12-13 4.821344599929567 1107 14-15 2.826522197819287 1107 16-17 1.4422002313877975 1107 18-19 2.090197472964242 1107 20-21 1.6756747860903651 1107 22-23 4.0520337798489585 1107 24-25 3.981614860804516 1107 26-27 2.9579036530430223 1107 28-29 3.7885719447268436 1107 30-31 3.048118726345809 1107 32-33 3.6298878915833477 1107 34-35 4.227080840609023 1107 36-37 4.526329818713627 1107 38-39 4.43779346452445 1107 40-41 2.8287225290960727 1107 42-43 3.5039944658997975 1107 44-45 3.9401838604339403 1107 46-47 4.343595621818949 1107 48-49 1.6700014210870897 1107 50-51 3.436224258915601 1107 52-53 3.648831694758215 1107 54-55 4.2217667986573915 1107 56-57 2.688434397079888 1107 58-59 1.807831481347268 1107 60-61 3.0891001236323525 1107 62-63 3.870854089570418 1107 64-65 4.077564759346242 1107 66-67 4.319512846310527 1107 68-69 4.123808552599744 1107 70-71 4.002698193589392 1107 72-73 3.9131947432022827 1107 74-75 4.0298084159613765 1107 76-77 3.890812948643717 1107 78-79 3.8338340495112284 1107 80-81 3.7807510294328495 1107 82-83 3.5875152972361235 1107 84-85 3.5066953265249587 1107 86-87 3.40073173988101 1107 88-89 3.4211579466643407 1107 90-91 3.5361074385376305 1107 92-93 3.535548664554778 1107 94-95 3.557714971177532 1107 96-97 3.455857942206901 1107 98-99 3.379236783926963 1107 100-101 3.446387001001 1108 1 -1.2606894180616557 1108 2 -0.6689507011566782 1108 3 -1.0986035240441119 1108 4 -2.707383661068633 1108 5 -7.917184863406327 1108 6 -6.116105229686639 1108 7 -4.605288562121757 1108 8 -5.054191378140466 1108 9 -7.075247770853778 1108 10-11 -2.7058401311921116 1108 12-13 -1.1303486371446354 1108 14-15 -5.980165235902874 1108 16-17 -5.135546257503389 1108 18-19 -3.371392580833966 1108 20-21 -4.05374327833816 1108 22-23 -2.8361551055194845 1108 24-25 -7.4123319520398 1108 26-27 -8.003858642303957 1108 28-29 -4.854431782997338 1108 30-31 -4.922449387871918 1108 32-33 -1.7736847262942064 1108 34-35 -0.32980251686452533 1108 36-37 -3.651729711715987 1108 38-39 -4.6541426242425885 1108 40-41 -2.3526071659612136 1108 42-43 -3.0354678430260513 1108 44-45 -0.8055505949885564 1108 46-47 -1.5252118459150594 1108 48-49 -0.5994751294909797 1108 50-51 -0.029854986745625922 1108 52-53 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2313 52-53 -3.069782435676572 2313 54-55 -6.115053853516528 2313 56-57 -4.61047864639837 2313 58-59 -4.86200547517447 2313 60-61 -2.9091335720198224 2313 62-63 -4.7345806930382786 2313 64-65 -5.397706979784193 2313 66-67 -7.229807805863388 2313 68-69 -8.181762099574168 2313 70-71 -6.802464849888867 2313 72-73 -8.083544387232497 2313 74-75 -8.480653540560361 2313 76-77 -7.407964225269325 2313 78-79 -8.130432254836595 2313 80-81 -8.992890274914977 2313 82-83 -8.007185789720399 2313 84-85 -7.8009133691272154 2313 86-87 -6.6022573905537705 2313 88-89 -8.578162705509575 2313 90-91 -8.281963213636281 2313 92-93 -7.558065465880006 2313 94-95 -8.347448072300727 2313 96-97 -7.645908362140926 2313 98-99 -7.843045824768691 2313 100-101 -7.456330390303346 2314 1 -11.092541088104877 2314 2 -13.297811727102772 2314 3 -11.423857389750088 2314 4 -25.311190918653402 2314 5 -18.116152760470595 2314 6 -16.224147395729993 2314 7 -16.157388752235033 2314 8 -11.351980739284976 2314 9 -13.621195279621038 2314 10-11 -11.521837934697349 2314 12-13 -13.653536632967969 2314 14-15 -15.04813385265198 2314 16-17 -15.751319835501835 2314 18-19 -14.534306555615874 2314 20-21 -14.785228033915715 2314 22-23 -14.652269389807474 2314 24-25 -15.66841079288961 2314 26-27 -14.4898112679056 2314 28-29 -16.922068637517 2314 30-31 -16.302056098829013 2314 32-33 -12.530775493944166 2314 34-35 -14.513560311716908 2314 36-37 -14.204176224156551 2314 38-39 -13.24017096235302 2314 40-41 -17.189900152570086 2314 42-43 -17.393031605854535 2314 44-45 -12.825434488608323 2314 46-47 -11.771347179944506 2314 48-49 -11.407720720901356 2314 50-51 -10.983091072492122 2314 52-53 -11.98759940131962 2314 54-55 -12.181092163033098 2314 56-57 -10.510357028885448 2314 58-59 -9.429442386089644 2314 60-61 -9.962280425166675 2314 62-63 -13.332999665370295 2314 64-65 -10.830665325786015 2314 66-67 -12.391665513373269 2314 68-69 -15.707088946640146 2314 70-71 -15.76071963857844 2314 72-73 -13.350707658743595 2314 74-75 -11.892769685285202 2314 76-77 -12.014288335941261 2314 78-79 -11.576327663775484 2314 80-81 -11.663793284948422 2314 82-83 -12.220974175247914 2314 84-85 -12.56684526331998 2314 86-87 -12.763704638957542 2314 88-89 -11.36739955561599 2314 90-91 -10.832814536226735 2314 92-93 -11.171549807625215 2314 94-95 -10.83443499841809 2314 96-97 -10.616233688987993 2314 98-99 -10.574666378128374 2314 100-101 -9.992161950047947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91902.0 3 13784.0 4 9394.0 5 7498.0 6 7933.0 7 9078.0 8 10621.0 9 11926.0 10 13377.0 11 14690.0 12 16678.0 13 18219.0 14 20578.0 15 22636.0 16 24886.0 17 26448.0 18 24205.0 19 21788.0 20 21839.0 21 23644.0 22 28139.0 23 34714.0 24 42252.0 25 51799.0 26 60696.0 27 69153.0 28 76896.0 29 85750.0 30 97720.0 31 108808.0 32 125886.0 33 152022.0 34 185828.0 35 228444.0 36 284181.0 37 354776.0 38 342560.0 39 89173.0 40 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.63228812429801 20.563029134643564 14.381255177109741 23.423427563948685 2 14.252817176780063 20.690412711954785 41.88660196935765 23.170168141907503 3 17.519610264651558 26.343708160675728 31.06667330972222 25.0700082649505 4 10.9729176359774 17.833040939416833 41.33674530711321 29.857296117492556 5 11.942392172701286 38.1175863792385 36.430549738861515 13.509471709198689 6 28.81290470443932 38.98761818395315 19.082384631382073 13.117092480225454 7 25.4056702216091 32.80217894978865 23.74740453829964 18.044746290302612 8 24.066227704959438 36.18094825067369 21.692382031104636 18.060442013262236 9 24.96276139098187 16.46698188457959 22.124349894005707 36.445906830432826 10-11 23.015764045011412 27.101863821327377 30.165478769125585 19.716893364535622 12-13 24.105256363972938 24.74188416579842 29.517798413962687 21.635061056265954 14-15 21.43345675976428 26.3525632066576 27.537297721888937 24.676682311689184 16-17 20.715458050309415 29.2552828281506 28.2478951653105 21.781363956229484 18-19 20.487383183690692 28.432998529828758 30.14990932233 20.92970896415055 20-21 21.568662476888363 27.80053617937354 30.0611876949699 20.569613648768197 22-23 21.159343238452603 27.600854326264766 29.750889893715048 21.48891254156758 24-25 21.028438762197876 27.77510442436747 29.925753872398104 21.270702941036554 26-27 20.87049986256961 27.960173680867335 29.824094406273264 21.345232050289788 28-29 20.786715287334957 27.985454833593543 29.91938041369074 21.308449465380765 30-31 21.238885763516645 27.67942879989244 29.830203833239572 21.251481603351344 32-33 21.015881224373143 27.910178302097687 29.635755498897815 21.438184974631355 34-35 21.2852541212826 28.057156143367056 29.628042345643447 21.0295473897069 36-37 21.222484907489985 27.63052325360247 29.648725732696402 21.498266106211144 38-39 21.089192233465205 28.03752106769774 29.76015862404117 21.113128074795878 40-41 21.331279556644585 27.626939918719117 29.72748017589045 21.314300348745853 42-43 21.21102678947268 27.79750025291775 29.556216010860958 21.43525694674861 44-45 21.274798396152576 28.014473106695746 29.395115954264107 21.315612542887568 46-47 21.262170002926727 27.92277690486906 29.156210183057357 21.658842909146856 48-49 21.196854359564217 28.009537795717293 29.366019188774505 21.427588655943985 50-51 21.13765747382325 28.08670796712114 29.105450545991772 21.67018401306384 52-53 21.19595702946779 28.335613381216696 28.93861766850839 21.529811920807123 54-55 21.23852057475589 28.089093152652765 28.93578078876617 21.736605483825176 56-57 21.251063072503758 28.27342136170415 28.746136336323346 21.729379229468744 58-59 21.143139868466747 28.241748326141057 29.00244583990057 21.612665965491622 60-61 21.3510470200308 28.287033784492838 28.79235626092867 21.569562934547687 62-63 21.16846753542286 28.225123965423606 28.82996003919403 21.776448459959514 64-65 21.08327388151969 28.198375066209707 28.85950043515547 21.858850617115124 66-67 21.147690319124614 28.204594096938436 28.720490964207713 21.927224619729238 68-69 21.310639524837786 28.087916599971095 28.72831775572142 21.8731261194697 70-71 21.30433215560439 27.82542756719645 28.803211139980135 22.067029137219027 72-73 21.339914905285223 27.985090580535914 28.727487373777528 21.94750714040133 74-75 21.249403273400958 28.274893878954337 28.593248182081915 21.88245466556279 76-77 21.288113206147088 28.27086564314142 28.55206477120504 21.888956379506457 78-79 21.361186817209614 28.234646856226963 28.412083576705914 21.99208274985751 80-81 21.30014491048306 28.314911221569577 28.366094551175024 22.01884931677234 82-83 21.578499538696327 28.213745966022184 28.462913552292683 21.7448409429888 84-85 21.67697930370177 28.177756859219873 28.27492921435621 21.870334622722147 86-87 21.319685387715932 28.279469813496217 28.46803718556351 21.932807613224345 88-89 21.61680311432098 28.079524442027505 28.355864952334308 21.94780749131721 90-91 21.554648142435592 28.153022077912443 28.450599164741767 21.841730614910198 92-93 21.699134600672856 28.1117503285309 28.365723529455416 21.823391541340833 94-95 21.408200569394666 28.099488944082786 28.39298479199639 22.099325694526158 96-97 21.550160546398388 28.015903050964603 28.398408776183093 22.03552762645392 98-99 21.464207181355064 28.063323160271135 28.22091905260134 22.251550605772465 100-101 21.52509007877321 28.015867715562738 28.229593893759876 22.22944831190418 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 188.0 1 126.5 2 87.5 3 124.0 4 115.0 5 121.5 6 194.5 7 346.0 8 646.0 9 905.5 10 935.5 11 956.5 12 1114.5 13 1314.5 14 1519.0 15 1743.5 16 1993.5 17 2271.5 18 2563.5 19 2898.0 20 3391.0 21 4074.5 22 4998.0 23 6418.0 24 8432.0 25 11074.0 26 15227.0 27 20780.5 28 26784.5 29 34452.5 30 42648.5 31 50643.5 32 60827.5 33 71075.5 34 80650.5 35 89562.0 36 100081.5 37 109467.0 38 111565.5 39 111964.0 40 112193.5 41 110858.5 42 109895.5 43 110270.0 44 110889.0 45 111382.0 46 109739.5 47 105609.0 48 101736.0 49 97268.0 50 91967.0 51 86545.0 52 80303.0 53 73753.0 54 67757.5 55 61141.5 56 53893.0 57 46677.5 58 40595.0 59 35506.5 60 29802.5 61 23652.0 62 18174.5 63 13832.5 64 10436.0 65 7596.5 66 5605.5 67 4284.0 68 3311.0 69 2612.0 70 2009.0 71 1503.0 72 1144.5 73 918.5 74 729.5 75 560.5 76 402.5 77 283.0 78 230.5 79 178.5 80 122.5 81 101.5 82 80.0 83 60.0 84 50.5 85 45.0 86 31.5 87 20.5 88 16.5 89 16.5 90 15.5 91 10.5 92 6.0 93 3.5 94 4.0 95 3.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051801699138134205 2 0.59798100580808 3 0.0 4 0.6624327788148718 5 0.5247307177362163 6 0.09367415035142825 7 0.10505214975284653 8 0.1077376402947962 9 0.10544083917339186 10-11 0.14706594257361158 12-13 0.3591843599857669 14-15 0.13349714825639225 16-17 0.12558201823801432 18-19 0.18310805247872544 20-21 0.10544083917339186 22-23 0.106006205603276 24-25 0.09673066261298936 26-27 0.17513991935754586 28-29 0.1096987550984568 30-31 0.13049363909763279 32-33 0.1028613548370455 34-35 0.15282561307805625 36-37 0.13673033752729216 38-39 0.13135935644339286 40-41 0.10727828007051532 42-43 0.10660690743502792 44-45 0.0908826536038753 46-47 0.09294977461313919 48-49 0.09699567812699755 50-51 0.08376257012752193 52-53 0.09176603865056927 54-55 0.3044674901935426 56-57 0.3247323431647022 58-59 0.3363753580801287 60-61 0.16252518089075602 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2830023.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.30337927599449 #Duplication Level Percentage of deduplicated Percentage of total 1 83.88008472234208 58.13173325215024 2 10.505186041332475 14.560897851746951 3 2.6996439066838884 5.61283336725123 4 1.0705669533508992 2.9677563043369313 5 0.5241192664981578 1.816161815598893 6 0.28809253420860725 1.1979471698904918 7 0.1815589015243237 0.8807851789291197 8 0.1299746728052567 0.7206147236556794 9 0.09701904877853876 0.6051373141045754 >10 0.5447501047587174 7.1918009774051885 >50 0.05428401842838292 2.6223825020642746 >100 0.023882062516588077 2.916557330013279 >500 6.283250791494633E-4 0.29726754998582927 >1k 1.5708126976588438E-4 0.27520472398190443 >5k 5.236042325529479E-5 0.2029199388853916 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5592 0.1975955672445065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4080 0.1441684396204554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.5335401867758674E-5 0.0 0.0 3.5335401867758674E-5 0.0 7 3.5335401867758674E-5 0.0 0.0 3.5335401867758674E-5 0.0 8 3.5335401867758674E-5 0.0 0.0 3.5335401867758674E-5 0.0 9 3.5335401867758674E-5 0.0 0.0 3.5335401867758674E-5 0.0 10-11 3.5335401867758674E-5 0.0 0.0 7.067080373551735E-5 0.0 12-13 3.5335401867758674E-5 1.7667700933879337E-5 0.0 1.0600620560327601E-4 0.0 14-15 5.300310280163801E-5 3.5335401867758674E-5 0.0 1.0600620560327601E-4 0.0 16-17 1.7667700933879336E-4 3.5335401867758674E-5 0.0 1.2367390653715535E-4 0.0 18-19 1.7667700933879336E-4 3.5335401867758674E-5 0.0 1.413416074710347E-4 0.0 20-21 1.7667700933879336E-4 3.5335401867758674E-5 0.0 1.7667700933879336E-4 0.0 22-23 1.7667700933879336E-4 3.5335401867758674E-5 0.0 2.473478130743107E-4 0.0 24-25 1.7667700933879336E-4 3.5335401867758674E-5 0.0 3.533540186775867E-4 0.0 26-27 1.943447102726727E-4 3.5335401867758674E-5 0.0 9.010527476278462E-4 0.0 28-29 2.1201241120655202E-4 7.067080373551735E-5 0.0 0.0023321365232720722 0.0 30-31 2.1201241120655202E-4 7.067080373551735E-5 0.0 0.00826848403705553 0.0 32-33 2.473478130743107E-4 7.067080373551735E-5 0.0 0.023180023625249686 0.0 34-35 3.533540186775867E-4 7.067080373551735E-5 0.0 0.04925755020365559 0.0 36-37 3.886894205453454E-4 7.067080373551735E-5 0.0 0.08906288040768573 0.0 38-39 4.2402482241310404E-4 7.067080373551735E-5 0.0 0.1448398122559428 0.0 40-41 4.5936022428086273E-4 7.067080373551735E-5 0.0 0.20328456694521563 0.0 42-43 4.946956261486214E-4 7.067080373551735E-5 0.0 0.2634607563260086 0.0 44-45 4.946956261486214E-4 7.067080373551735E-5 0.0 0.34015624607997885 0.0 46-47 4.946956261486214E-4 7.067080373551735E-5 0.0 0.4314099214034656 0.0 48-49 4.946956261486214E-4 7.067080373551735E-5 0.0 0.5246423792315469 0.0 50-51 4.946956261486214E-4 7.067080373551735E-5 0.0 0.62170872816228 0.0 52-53 4.946956261486214E-4 7.067080373551735E-5 0.0 0.7199234776537151 0.0 54-55 4.946956261486214E-4 7.067080373551735E-5 0.0 0.8249049566028261 0.0 56-57 5.123633270825007E-4 7.067080373551735E-5 0.0 0.9522537449342284 0.0 58-59 5.3003102801638E-4 7.067080373551735E-5 0.0 1.0942137219379489 0.0 60-61 5.3003102801638E-4 7.067080373551735E-5 0.0 1.2437354749413698 0.0 62-63 5.3003102801638E-4 7.067080373551735E-5 0.0 1.391737805664477 0.0 64-65 5.653664298841388E-4 7.067080373551735E-5 0.0 1.5454291360882935 0.0 66-67 5.653664298841388E-4 7.067080373551735E-5 0.0 1.721470108193467 0.0 68-69 5.653664298841388E-4 7.067080373551735E-5 0.0 1.9208854486341629 0.0 70-71 5.830341308180182E-4 7.067080373551735E-5 0.0 2.135088654756516 0.0 72-73 6.007018317518974E-4 7.067080373551735E-5 0.0 2.3505462676451745 0.0 74-75 6.007018317518974E-4 7.067080373551735E-5 0.0 2.5709155013934515 0.0 76-77 6.007018317518974E-4 7.067080373551735E-5 0.0 2.8182279790658944 0.0 78-79 6.007018317518974E-4 7.067080373551735E-5 0.0 3.0866533593543233 0.0 80-81 6.007018317518974E-4 7.067080373551735E-5 0.0 3.3750962448008375 0.0 82-83 6.007018317518974E-4 7.067080373551735E-5 0.0 3.67988175361119 0.0 84-85 6.007018317518974E-4 8.833850466939668E-5 0.0 3.976098427468611 0.0 86-87 6.007018317518974E-4 1.0600620560327601E-4 0.0 4.291890913960771 0.0 88-89 6.007018317518974E-4 1.0600620560327601E-4 0.0 4.634838656788302 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3205 0.0 54.853226 1 GTATCAA 6525 0.0 44.585514 1 ATCAACG 7780 0.0 36.20583 3 CAACGCA 7905 0.0 35.657818 5 TCAACGC 8060 0.0 35.371056 4 AACGCAG 8235 0.0 34.548767 6 TATCAAC 8545 0.0 33.42686 2 ACGCAGA 8835 0.0 31.244896 7 CGCAGAG 8885 0.0 30.639786 8 GCAGAGT 10000 0.0 25.650116 9 GTACATG 6975 0.0 22.926155 1 TACATGG 6980 0.0 21.87544 2 TATACCG 265 7.548806E-10 21.597555 5 CAGAGTA 10030 0.0 21.318327 10-11 GAGTACT 6540 0.0 21.222712 12-13 ACATGGG 6855 0.0 21.03487 3 AGAGTAC 9435 0.0 20.944704 10-11 GTACTTT 7390 0.0 19.034779 14-15 GTGGTAT 1970 0.0 17.848183 1 GTGTAGC 1930 0.0 17.469404 1 >>END_MODULE