FastQCFastQC Report
Sat 18 Jun 2016
SRR1931146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830023
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64780.22890273329934066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.18187131341335389No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.11353264620110862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35950.055.4503251
GTATCAA67500.042.4308471
ATCAACG81550.034.416083
CAACGCA82950.033.9479185
TCAACGC85000.033.017444
AACGCAG87850.031.8381926
TATCAAC89600.031.5890162
ACGCAGA99000.028.0605097
CGCAGAG99850.027.1558178
GCAGAGT113950.023.2955159
GAGTACT73050.021.06212-13
AGAGTAC104800.020.61697810-11
CAGAGTA111450.020.34549110-11
GTACATG75950.020.1078781
TACATGG74700.020.0257052
TGGTATC16700.019.905792
GTACTTT78700.019.64043614-15
GTGGTAT18050.019.5048891
ACATGGG73600.019.4861933
GTACTAG9650.019.2276541
Produced by FastQC (version 0.11.5)