##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931146_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2830023 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33937886723889 31.0 31.0 34.0 30.0 34.0 2 31.37628952132191 31.0 31.0 34.0 30.0 34.0 3 31.522183741969588 31.0 31.0 34.0 30.0 34.0 4 35.09732005711614 37.0 35.0 37.0 32.0 37.0 5 35.01994224075211 35.0 35.0 37.0 32.0 37.0 6 35.133404922857515 37.0 35.0 37.0 32.0 37.0 7 35.01128224046236 36.0 35.0 37.0 32.0 37.0 8 35.05121760494526 36.0 35.0 37.0 32.0 37.0 9 36.408069828407754 38.0 35.0 39.0 32.0 39.0 10-11 36.32899697281612 38.0 35.0 39.0 32.0 39.0 12-13 36.25444033493721 38.0 35.0 39.0 32.0 39.0 14-15 37.20900236499845 39.0 36.0 41.0 31.5 41.0 16-17 37.28175725073613 39.0 36.0 41.0 32.0 41.0 18-19 37.35527167093695 39.0 36.0 41.0 32.0 41.0 20-21 37.323103557815614 39.0 36.0 41.0 31.5 41.0 22-23 37.22590576118993 39.0 36.0 40.0 31.0 41.0 24-25 37.10998126163639 39.0 36.0 40.0 31.0 41.0 26-27 36.867406201292354 39.0 36.0 40.0 30.5 41.0 28-29 36.60473837138426 39.0 35.0 40.0 30.0 41.0 30-31 36.399889859552374 38.0 35.0 40.0 30.0 41.0 32-33 36.24207577111564 38.0 35.0 40.0 30.0 41.0 34-35 36.025608095764596 38.0 35.0 40.0 29.5 41.0 36-37 36.263102455351074 38.0 35.0 40.0 30.0 41.0 38-39 36.26674270845149 38.0 35.0 40.0 30.0 41.0 40-41 36.11966439848722 38.0 35.0 40.0 30.0 41.0 42-43 35.96447361028514 38.0 35.0 40.0 29.0 41.0 44-45 35.75902068640431 38.0 35.0 40.0 28.5 41.0 46-47 35.576560861872856 38.0 34.5 40.0 27.5 41.0 48-49 35.34110588500518 38.0 34.0 40.0 27.0 41.0 50-51 35.03462516029022 38.0 34.0 40.0 26.0 41.0 52-53 34.90410872985838 38.0 34.0 40.0 26.0 41.0 54-55 34.71875316914385 38.0 33.0 40.0 26.0 41.0 56-57 34.50410986059124 38.0 33.0 40.0 25.0 41.0 58-59 34.29123243874696 37.5 33.0 40.0 25.0 41.0 60-61 34.02096891085337 37.0 33.0 40.0 24.0 41.0 62-63 33.79038809931934 37.0 32.5 40.0 23.5 41.0 64-65 33.5825060432371 36.5 32.0 39.5 23.0 41.0 66-67 33.349053346916264 36.0 32.0 39.0 23.0 41.0 68-69 33.02592841118252 36.0 31.5 39.0 22.0 40.0 70-71 32.20987691619467 35.0 31.0 39.0 19.0 40.0 72-73 32.19102194575804 35.0 31.0 38.0 21.0 40.0 74-75 31.878104170884832 35.0 31.0 37.5 20.5 39.5 76-77 31.134425939294488 34.0 30.0 36.5 20.0 39.0 78-79 31.137250651319796 34.0 30.0 36.0 19.0 39.0 80-81 30.861180986868305 34.0 30.0 36.0 18.0 38.5 82-83 30.48559958699982 34.0 30.0 36.0 17.5 37.0 84-85 30.118621297424085 34.0 30.0 35.0 14.0 37.0 86-87 29.787139009117595 34.0 29.0 35.0 10.5 36.5 88-89 29.469488410518217 34.0 29.0 35.0 7.0 36.0 90-91 29.161881193191718 34.0 29.0 35.0 4.0 36.0 92-93 28.835158583516815 34.0 29.0 35.0 2.0 35.5 94-95 28.453482356857172 34.0 29.0 35.0 2.0 35.0 96-97 28.106143836993553 34.0 29.0 35.0 2.0 35.0 98-99 27.647587493105178 33.0 27.5 35.0 2.0 35.0 100-101 26.974157277167006 33.0 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8215512668618992 1101 2 0.751953628478347 1101 3 0.5934986211203608 1101 4 0.8279156250772388 1101 5 0.804019380768807 1101 6 0.805772206487994 1101 7 0.7268408049992132 1101 8 0.7767564225219132 1101 9 1.1646365231169398 1101 10-11 0.9942305323521055 1101 12-13 1.098287166100576 1101 14-15 1.4033867781768379 1101 16-17 1.1863052822914781 1101 18-19 1.0179757616626546 1101 20-21 0.9839425435225735 1101 22-23 0.7979301315806993 1101 24-25 0.8357824668002536 1101 26-27 1.0891039788550145 1101 28-29 1.0349286428247524 1101 30-31 1.1271998035658868 1101 32-33 1.1688117597149983 1101 34-35 0.8515209460236335 1101 36-37 1.2584070659554385 1101 38-39 1.0734892380527654 1101 40-41 1.0013457262312073 1101 42-43 1.1227323375803664 1101 44-45 0.984570672172346 1101 46-47 1.009549588592428 1101 48-49 0.9489604759272012 1101 50-51 0.8154209404281758 1101 52-53 0.7782948229708637 1101 54-55 0.6447885967470484 1101 56-57 0.6206880145936964 1101 58-59 0.7864184186244643 1101 60-61 0.9644391453533387 1101 62-63 0.7457055645434849 1101 64-65 0.7184858481509053 1101 66-67 0.4861511476961553 1101 68-69 0.47160339970612597 1101 70-71 0.7274439816635194 1101 72-73 0.2758599007914775 1101 74-75 -0.004052955348790022 1101 76-77 0.026417866656487377 1101 78-79 0.05016925295600316 1101 80-81 0.07186128419433757 1101 82-83 -0.04895232709568731 1101 84-85 -0.09110320163746266 1101 86-87 -0.06846455910879001 1101 88-89 -0.21777019663419495 1101 90-91 -0.11578882813325464 1101 92-93 -0.3277922592991729 1101 94-95 -0.30650933441797434 1101 96-97 -0.2750357381674462 1101 98-99 -0.32010382511233715 1101 100-101 -0.36754263586591307 1102 1 1.026504326371633 1102 2 0.9512705184453054 1102 3 0.7142828350438464 1102 4 0.8694981129156361 1102 5 0.9465227432886323 1102 6 0.8638687818649657 1102 7 0.9231121357079033 1102 8 0.9648981465616018 1102 9 1.2553534580017427 1102 10-11 1.2249192520605732 1102 12-13 1.2893351341748769 1102 14-15 1.6577938989180439 1102 16-17 1.522023907070995 1102 18-19 1.3633203839307484 1102 20-21 1.3801015669959327 1102 22-23 0.9742540705820772 1102 24-25 0.7741975561616528 1102 26-27 1.1342146484256617 1102 28-29 1.2171504491722445 1102 30-31 1.3716983369967366 1102 32-33 1.4965869947881174 1102 34-35 1.4025788908791483 1102 36-37 1.585478079947869 1102 38-39 1.3207377465901047 1102 40-41 1.307880770654549 1102 42-43 1.442397594372757 1102 44-45 1.5012350474563405 1102 46-47 1.4648225781947701 1102 48-49 1.193779613386745 1102 50-51 1.1555308695115656 1102 52-53 1.1058049273787205 1102 54-55 1.0103984768858254 1102 56-57 1.276390330962542 1102 58-59 1.2905447818076112 1102 60-61 1.5406693187857314 1102 62-63 1.374938156900953 1102 64-65 1.3523439586599153 1102 66-67 1.0482423528134959 1102 68-69 1.0564771824301502 1102 70-71 1.4035198911276154 1102 72-73 0.7944627057808198 1102 74-75 0.7375150302276268 1102 76-77 0.8266920659740933 1102 78-79 0.8161589129232354 1102 80-81 0.710092755459133 1102 82-83 0.8432096868005381 1102 84-85 0.8627176242004388 1102 86-87 0.8068821599429299 1102 88-89 0.6091604912304582 1102 90-91 0.6799963672096467 1102 92-93 0.5408495750133042 1102 94-95 0.5949746686140642 1102 96-97 0.6459162984758038 1102 98-99 0.7488728585526836 1102 100-101 0.5097950588447482 1103 1 0.607401771123957 1103 2 0.3721449553483751 1103 3 0.2615127954522265 1103 4 0.5774795145328255 1103 5 0.6017547597316266 1103 6 0.5351836001593213 1103 7 0.5056332173656344 1103 8 0.6392426369936857 1103 9 0.7348291312156903 1103 10-11 0.7118394688238538 1103 12-13 0.8720425016790117 1103 14-15 1.2544510850173083 1103 16-17 1.0107425764941098 1103 18-19 0.8098629475029071 1103 20-21 0.8076283478040409 1103 22-23 0.521357571163378 1103 24-25 0.3717192076106386 1103 26-27 0.7728893395356806 1103 28-29 0.42667701246120515 1103 30-31 0.7839233962100636 1103 32-33 0.9603177800116356 1103 34-35 0.726578478120878 1103 36-37 1.0738380614405898 1103 38-39 0.8479076094928004 1103 40-41 0.773522440194931 1103 42-43 0.8669459883824686 1103 44-45 0.9003734950148825 1103 46-47 0.9250184296027868 1103 48-49 0.7910294484113365 1103 50-51 0.6464697444011662 1103 52-53 0.272066819450437 1103 54-55 0.5471768317165129 1103 56-57 0.6552444778505233 1103 58-59 0.7824730965383466 1103 60-61 0.9512666357909296 1103 62-63 0.7298318798972332 1103 64-65 0.5965636305608228 1103 66-67 0.4291246430162232 1103 68-69 0.3999829607571712 1103 70-71 0.6789489819049734 1103 72-73 0.24129198039323185 1103 74-75 0.004320736689162885 1103 76-77 0.20620776485793968 1103 78-79 0.23659973496410203 1103 80-81 -0.04129646659879427 1103 82-83 0.05171603079288545 1103 84-85 0.1540105123390667 1103 86-87 0.0903831629158276 1103 88-89 -0.11962955517225637 1103 90-91 -0.06712480473998994 1103 92-93 -0.40290022745768184 1103 94-95 -0.34911647224171816 1103 96-97 -0.5030112933090649 1103 98-99 -0.5894245077904223 1103 100-101 -0.7491095490212736 1104 1 0.44646731806569306 1104 2 0.4685089129732418 1104 3 0.19460752107473311 1104 4 0.6030398935407604 1104 5 0.40571561923236743 1104 6 0.5336571235380987 1104 7 0.5266562025055492 1104 8 0.5120605947710217 1104 9 0.7764604413351748 1104 10-11 0.563556781740516 1104 12-13 0.6544759440921979 1104 14-15 0.9413591522948579 1104 16-17 0.7631334628052642 1104 18-19 0.6845369013587828 1104 20-21 0.6037938456562912 1104 22-23 0.34186529474321503 1104 24-25 0.32906086549606073 1104 26-27 0.13646685925518653 1104 28-29 0.5473167575663282 1104 30-31 0.7609566025091894 1104 32-33 0.9911041594940713 1104 34-35 0.6279622104755518 1104 36-37 0.9735307355640117 1104 38-39 0.4599358534070177 1104 40-41 0.6327609901953224 1104 42-43 0.8699467528676053 1104 44-45 0.766479942980375 1104 46-47 0.733964116959001 1104 48-49 0.6248123531556402 1104 50-51 0.6480703183706282 1104 52-53 0.34940824221927613 1104 54-55 0.6472339009241423 1104 56-57 0.7166068787186006 1104 58-59 0.6304933792624752 1104 60-61 0.9058456737936069 1104 62-63 0.018695048456592644 1104 64-65 -0.8680263866739395 1104 66-67 -0.34784322937104406 1104 68-69 0.08269669063956542 1104 70-71 0.7707196164133947 1104 72-73 0.3360115468667466 1104 74-75 0.11485848394453058 1104 76-77 -0.2184428910817111 1104 78-79 -0.15357071743583361 1104 80-81 -0.07928382587617477 1104 82-83 -0.31039094155384106 1104 84-85 -0.3178364139390588 1104 86-87 -0.11483496471542765 1104 88-89 -0.13359761779021184 1104 90-91 0.02460325941691366 1104 92-93 0.08126003940676796 1104 94-95 0.1498037726500172 1104 96-97 0.21018957676479388 1104 98-99 0.07782389430738945 1104 100-101 -0.2813682570627627 1105 1 0.0295033444873809 1105 2 -1.3845975628939975 1105 3 -0.19720508272486725 1105 4 0.47286928083960333 1105 5 0.6155558288526919 1105 6 0.05094143906002557 1105 7 -0.08223252678018156 1105 8 0.06423360704420134 1105 9 0.5998019727458441 1105 10-11 0.9175878816425751 1105 12-13 1.0135672312639983 1105 14-15 1.210767402120446 1105 16-17 0.9723452350110549 1105 18-19 0.7308235345026475 1105 20-21 1.0707330347065351 1105 22-23 0.40867060130251076 1105 24-25 -0.1596659648068126 1105 26-27 0.8622430446845897 1105 28-29 0.3830881518318492 1105 30-31 0.8550353869224665 1105 32-33 1.140735921258127 1105 34-35 0.6090240379746277 1105 36-37 0.831327211465009 1105 38-39 0.7396245413187117 1105 40-41 0.8755978625751055 1105 42-43 1.2343640279445651 1105 44-45 1.2496153223647397 1105 46-47 1.310122475207308 1105 48-49 1.0091858539986163 1105 50-51 0.730890757764243 1105 52-53 0.9582315955981429 1105 54-55 0.24214133263011206 1105 56-57 0.4621214316103206 1105 58-59 -0.34427707610679903 1105 60-61 0.38995923634776375 1105 62-63 0.967704143073945 1105 64-65 1.3495231770109442 1105 66-67 1.0264987836114443 1105 68-69 0.9236726823352939 1105 70-71 0.5031480612556223 1105 72-73 -0.12332565193218059 1105 74-75 -0.08028202975049226 1105 76-77 0.13813308109332212 1105 78-79 -0.5043644515388266 1105 80-81 -0.34573733025498044 1105 82-83 -0.39778662017570454 1105 84-85 -0.16469243133089506 1105 86-87 -0.44617353487375055 1105 88-89 -0.6540050600852325 1105 90-91 -0.7533782272312095 1105 92-93 -0.2868764495264422 1105 94-95 -0.5263168946998746 1105 96-97 -0.509281977195954 1105 98-99 -1.1801558182903022 1105 100-101 -0.5097278650651411 1106 1 0.3544093042477492 1106 2 0.3293420541850125 1106 3 -0.28568948736916866 1106 4 0.3288117052100574 1106 5 0.021686065447134695 1106 6 0.42485683268775887 1106 7 0.3016522388015943 1106 8 0.46701836504776395 1106 9 0.8164997234924343 1106 10-11 0.12867711877152033 1106 12-13 0.2628521632981702 1106 14-15 -0.021069402100820867 1106 16-17 -0.3095526978808678 1106 18-19 0.34760783110714044 1106 20-21 0.442801658429552 1106 22-23 0.4285216927996913 1106 24-25 0.2833460188269328 1106 26-27 0.7335310120271927 1106 28-29 0.5712582954221901 1106 30-31 0.20351468130379402 1106 32-33 0.2938477830369237 1106 34-35 -1.1380791739128426 1106 36-37 -0.8403144504568942 1106 38-39 -0.7745594898471921 1106 40-41 0.5254560561306789 1106 42-43 0.6808123951752947 1106 44-45 0.5585705217219967 1106 46-47 0.22215221532830043 1106 48-49 0.17777624630183908 1106 50-51 0.7815513363774613 1106 52-53 0.14008446160396915 1106 54-55 0.693717935941585 1106 56-57 0.4926696045571717 1106 58-59 0.8055826934485069 1106 60-61 0.9679498299765683 1106 62-63 0.8069070694707392 1106 64-65 0.786987373314787 1106 66-67 0.5197444846530104 1106 68-69 0.6075276045177844 1106 70-71 1.0157863049346858 1106 72-73 0.3569747537368535 1106 74-75 0.46663293399816297 1106 76-77 0.5366564364034687 1106 78-79 0.4610241128729555 1106 80-81 0.45662254492584253 1106 82-83 0.4042573508875975 1106 84-85 0.5184189542947841 1106 86-87 0.5065664580308962 1106 88-89 0.35090800173352577 1106 90-91 0.4779801437081588 1106 92-93 0.07470148164864554 1106 94-95 0.22613666863553306 1106 96-97 0.546738744182715 1106 98-99 0.44306932098448115 1106 100-101 0.260475244415872 1107 1 0.33478725482614635 1107 2 0.3561100576347762 1107 3 0.0850393219001937 1107 4 -1.457735391552042 1107 5 -0.07435432396358976 1107 6 0.24919816703351927 1107 7 0.3548326060888414 1107 8 0.4204772678731956 1107 9 0.7548530242030083 1107 10-11 0.5995406844013829 1107 12-13 0.7432687830930576 1107 14-15 0.8460003032631818 1107 16-17 0.8714907147595099 1107 18-19 0.6358471162187911 1107 20-21 0.12126806073939633 1107 22-23 0.3855098061169002 1107 24-25 0.3336846674198455 1107 26-27 0.6391652926391487 1107 28-29 0.503044918685589 1107 30-31 0.8343521510714922 1107 32-33 0.9693642514359624 1107 34-35 0.7697744267771114 1107 36-37 1.0201592825989607 1107 38-39 0.8149294554440658 1107 40-41 0.26487938093334407 1107 42-43 0.13337089115370304 1107 44-45 -0.12178381719844822 1107 46-47 0.3815277426357966 1107 48-49 0.47789190241297774 1107 50-51 -1.0458286367181415 1107 52-53 0.2892299305092294 1107 54-55 0.7174134943719395 1107 56-57 0.7076354115954402 1107 58-59 0.7935324867439704 1107 60-61 1.1115098820979838 1107 62-63 1.1478955015160324 1107 64-65 0.8804467097041595 1107 66-67 0.830972970507112 1107 68-69 0.8167302335557878 1107 70-71 1.3283137249245627 1107 72-73 0.8035725071725572 1107 74-75 0.6929806147826056 1107 76-77 0.5805588282702772 1107 78-79 0.5282622050978674 1107 80-81 0.6076196834038221 1107 82-83 0.7153335620543295 1107 84-85 0.48968878105669233 1107 86-87 0.598202775221587 1107 88-89 0.5804331203191211 1107 90-91 0.6228270604826349 1107 92-93 0.5791715844853229 1107 94-95 0.6908552856368644 1107 96-97 0.7985631695347237 1107 98-99 0.728182727250303 1107 100-101 0.6239884950673904 1108 1 0.56216016303296 1108 2 0.48018798743846247 1108 3 0.2702438456050267 1108 4 0.6191711469990295 1108 5 0.474348408623122 1108 6 0.45053778760099306 1108 7 0.5227963246311162 1108 8 0.6609691464134002 1108 9 0.7330479392380553 1108 10-11 0.6602434234542258 1108 12-13 0.8094362106077355 1108 14-15 1.00736638646935 1108 16-17 0.8456213435260338 1108 18-19 0.6764949901551134 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62-63 2.113660068212276 2311 64-65 -1.241078911413851 2311 66-67 1.2927390899983848 2311 68-69 1.6058819268418034 2311 70-71 2.0179987293611177 2311 72-73 1.4473030972346699 2311 74-75 1.4044324781366413 2311 76-77 1.7068278684950684 2311 78-79 1.619192218407541 2311 80-81 1.48752300342748 2311 82-83 1.5090086951510493 2311 84-85 1.6641212714441487 2311 86-87 1.5785987379545077 2311 88-89 1.707346385625243 2311 90-91 1.904459713543858 2311 92-93 1.8142989506491816 2311 94-95 2.1396250652878557 2311 96-97 2.1259006806261134 2311 98-99 2.3645273738045773 2311 100-101 2.330308740556891 2312 1 -0.1815783228598704 2312 2 -0.13872234459986288 2312 3 -0.35536530120724663 2312 4 0.15480104274477924 2312 5 -1.1466850056897684 2312 6 -0.22680023390567072 2312 7 0.1656925567001295 2312 8 -2.7227631580032536 2312 9 -5.2892203301812515 2312 10-11 -0.8187736221324968 2312 12-13 -5.747972310745496 2312 14-15 -2.2363718224756184 2312 16-17 -3.5577609652663895 2312 18-19 -2.669418145703318 2312 20-21 -3.24551094500913 2312 22-23 -1.9578125969273898 2312 24-25 -3.4204435408224043 2312 26-27 -3.6232231273268454 2312 28-29 -5.450252132160493 2312 30-31 -5.706798110040964 2312 32-33 -1.2285551984061485 2312 34-35 0.5450304789186404 2312 36-37 0.8587340943550927 2312 38-39 0.5062173572333961 2312 40-41 -3.258645283061476 2312 42-43 -4.905681614716272 2312 44-45 -3.91571826582609 2312 46-47 -0.31447561741601504 2312 48-49 -1.783457887077752 2312 50-51 -6.304029642709558 2312 52-53 -1.6401768482941037 2312 54-55 0.11946191150416041 2312 56-57 0.660228825084225 2312 58-59 0.917721064591916 2312 60-61 1.1746272410162888 2312 62-63 1.0403616985850945 2312 64-65 0.9635100750187107 2312 66-67 0.8152773790570293 2312 68-69 1.0679824094929629 2312 70-71 1.3974518513920557 2312 72-73 0.8749035228797872 2312 74-75 0.8237195225633513 2312 76-77 0.9372462928480445 2312 78-79 0.8074584746306961 2312 80-81 0.8689029296996686 2312 82-83 -0.29780732734733917 2312 84-85 0.09949922150685708 2312 86-87 0.7381757531181172 2312 88-89 0.4986244611012438 2312 90-91 0.9837209152312383 2312 92-93 1.1316002845905793 2312 94-95 1.0896668697191245 2312 96-97 1.4364981039529887 2312 98-99 1.3835825936578807 2312 100-101 1.3625931627056396 2313 1 -0.31222361473907156 2313 2 -0.46019429019131053 2313 3 -0.4209389389693712 2313 4 -1.177844087204214 2313 5 -2.0528869326592414 2313 6 -2.733682267749085 2313 7 -2.1894608721720275 2313 8 -3.8648488190833277 2313 9 -3.3212339323187337 2313 10-11 -1.6743723590768766 2313 12-13 -3.857546434298243 2313 14-15 -2.3476138271715996 2313 16-17 -5.67144406784918 2313 18-19 -4.226381144650773 2313 20-21 -5.6260145479562595 2313 22-23 -3.3242728025787542 2313 24-25 -0.9527283760584169 2313 26-27 -3.0188510117086764 2313 28-29 -4.625080081314408 2313 30-31 -4.18616415327633 2313 32-33 -6.101423792042297 2313 34-35 -2.6829429645272356 2313 36-37 -5.380384195661911 2313 38-39 -1.964554240208109 2313 40-41 -1.4276477929796982 2313 42-43 -4.337824761020212 2313 44-45 -5.646104469372361 2313 46-47 -2.9592331269294156 2313 48-49 -1.2756407062826725 2313 50-51 0.025231145890558082 2313 52-53 -1.1576450694907692 2313 54-55 -3.6869343317150154 2313 56-57 -5.4910058927523835 2313 58-59 -0.9291577306473329 2313 60-61 -7.411859683119406 2313 62-63 -2.058626237614405 2313 64-65 -2.341700029426274 2313 66-67 -4.293211812101582 2313 68-69 -5.461938244705081 2313 70-71 -3.9901645359450058 2313 72-73 0.026806202824730008 2313 74-75 0.3280349626086938 2313 76-77 -2.9793596499905917 2313 78-79 -0.9009497514238731 2313 80-81 0.5091142486212128 2313 82-83 0.6641107359673732 2313 84-85 0.3426779114914744 2313 86-87 0.3419527752215892 2313 88-89 -3.522556010115661 2313 90-91 -0.02934685256084535 2313 92-93 0.4301226714418469 2313 94-95 0.9146324595499067 2313 96-97 1.2750577347521102 2313 98-99 1.2018240315981288 2313 100-101 1.7916515385456506 2314 1 -28.842658397347765 2314 2 -14.901057774533058 2314 3 -5.13845186083012 2314 4 -3.027798480636868 2314 5 -1.448295871546442 2314 6 -1.3250474243316361 2314 7 -0.29253171437330394 2314 8 -3.985827840062349 2314 9 -2.320747462267043 2314 10-11 -0.5985134353918653 2314 12-13 0.5684645112779236 2314 14-15 -1.5423386675486128 2314 16-17 -1.0576860866998956 2314 18-19 -3.0464024277155346 2314 20-21 -2.426668242088212 2314 22-23 -4.589748631098061 2314 24-25 -0.6936070625892796 2314 26-27 -4.270720880969851 2314 28-29 -2.274456791560688 2314 30-31 -3.3767692004031105 2314 32-33 -0.8227068568036202 2314 34-35 -0.919473824971142 2314 36-37 -5.346133663585292 2314 38-39 -8.523538733975208 2314 40-41 -5.970548370662883 2314 42-43 -2.9314398415918106 2314 44-45 -4.8558186682169975 2314 46-47 -5.57656362252078 2314 48-49 -5.7991888972221695 2314 50-51 -2.349213974206741 2314 52-53 -2.8135435188674798 2314 54-55 -4.932236855278408 2314 56-57 -2.4095677656620857 2314 58-59 -2.969306712100664 2314 60-61 -1.011494830580645 2314 62-63 0.5945054133433345 2314 64-65 -0.7356343559692959 2314 66-67 -0.25838359683858414 2314 68-69 0.39583007393280667 2314 70-71 0.9038914081109439 2314 72-73 0.6629874129189837 2314 74-75 0.15383307753724296 2314 76-77 -0.5072556670170449 2314 78-79 -1.0399129621262162 2314 80-81 -0.9552366179035694 2314 82-83 -2.0418333199766785 2314 84-85 -0.9059538914881529 2314 86-87 0.41940792876370026 2314 88-89 0.38589640703240846 2314 90-91 0.5805193681749401 2314 92-93 0.8199980134911016 2314 94-95 0.8426196897110501 2314 96-97 1.3240239858922749 2314 98-99 1.161720656712145 2314 100-101 0.9719647036414258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 38.0 5 98.0 6 230.0 7 555.0 8 1505.0 9 2764.0 10 4308.0 11 6406.0 12 8560.0 13 10638.0 14 13012.0 15 15770.0 16 17959.0 17 20186.0 18 18496.0 19 15734.0 20 15355.0 21 16341.0 22 18042.0 23 21048.0 24 24940.0 25 30602.0 26 39045.0 27 50064.0 28 63389.0 29 79871.0 30 98662.0 31 121619.0 32 147655.0 33 179766.0 34 222469.0 35 273697.0 36 346755.0 37 438517.0 38 411353.0 39 94464.0 40 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.83338802346331 21.745255852463373 15.194617216877692 23.226738907195628 2 14.604828299982014 21.896571158608957 40.9354270265648 22.563173514844227 3 17.717307147978516 26.894172432229386 31.380445157157006 24.00807526263509 4 12.094520986976404 18.143459915611814 40.04276299400322 29.719256103408565 5 11.665953477952222 38.91187072285533 36.84202157375249 12.580154225439955 6 29.08654805985676 39.9569544134447 18.30879112996608 12.647706396732463 7 25.42357429603929 33.471247406823196 23.66871223308079 17.436466064056724 8 24.193937646443157 36.82973601274618 21.094634213220175 17.881692127590483 9 24.81753681860536 16.35124520189412 21.83112999434987 37.00008798515065 10-11 23.108734451981487 27.491578690349865 29.96230065974729 19.43738619792136 12-13 24.01505217448763 25.074195510071828 29.639812114601188 21.27094020083936 14-15 21.34855441104189 26.836796026039366 27.398752589643266 24.415896973275483 16-17 20.682287741124366 29.74087136394298 28.10235111163408 21.47448978329858 18-19 20.533684708569506 28.837733827604932 30.03549794471635 20.59308351910921 20-21 21.434737456197354 28.050584747897812 30.00961476284822 20.505063033056622 22-23 21.13883880095674 27.95325338345307 29.70467024472946 21.20323757086073 24-25 21.04769094615648 28.067151719243483 29.917483805662293 20.96767352893775 26-27 20.978980821267186 28.27298463573978 29.706663029123785 21.04137151386925 28-29 20.915161789897088 28.203344550153673 29.86307469271537 21.018418967233867 30-31 21.372270485822163 27.929924433160362 29.762027177206622 20.935777903810852 32-33 21.172331923490013 28.22484654062471 29.504359301034793 21.098462234850484 34-35 21.359331543257507 28.165229994471463 29.608056790058452 20.867381672212577 36-37 21.356296583957928 27.85341263214358 29.633801901150225 21.156488882748267 38-39 21.16502075535536 28.164491602009544 29.68424827980993 20.986239362825167 40-41 21.396141441036345 27.93141556763079 29.695992056375704 20.976450934957157 42-43 21.286332339034868 27.927112969631995 29.53398597899588 21.252568712337258 44-45 21.340861953911315 28.189040955999833 29.30737998102827 21.16271710906058 46-47 21.351355602769868 28.092721507968786 29.0814471616534 21.47447572760795 48-49 21.332458248356257 28.10846459166206 29.175813796336698 21.383263363644993 50-51 21.400971556911905 28.168855183696973 28.95300428095469 21.47716897843643 52-53 21.374499640985338 28.286909957992457 28.86417796950354 21.47441243151866 54-55 21.478411530958823 28.165761774889887 28.87114080698118 21.48468588717011 56-57 21.513719397192112 28.27531692255395 28.695230141313242 21.515733538940694 58-59 21.296001175979214 28.21823601704887 29.010202185031563 21.47556062194035 60-61 21.42698379380799 28.30219448985231 28.791097654833763 21.47972406150593 62-63 21.388759418595736 28.27658294071148 28.738364900330648 21.596292740362138 64-65 21.218574331924604 28.23001322032856 28.72772802239646 21.82368442535038 66-67 21.339485592017965 28.270406080876935 28.588832289993654 21.801276037111442 68-69 21.420468087640064 28.116195220323668 28.680019145136782 21.78331754689949 70-71 21.427073378144605 27.850552066288593 28.666809076110784 22.055565479456025 72-73 21.439684907259878 27.969410194081266 28.650177020577072 21.94072787808178 74-75 21.30045298610582 28.203707528466886 28.492576939528412 22.003262545898874 76-77 21.22016322835539 28.210336099741944 28.45167689449874 22.11782377740393 78-79 21.307689018781826 28.153764121351664 28.33810891289576 22.200437946970748 80-81 21.207276499820054 28.224590368706593 28.291498153101337 22.27663497837202 82-83 21.40904598469191 28.06938286725086 28.43447030343034 22.087100844626885 84-85 21.430143853954544 28.058959238140467 28.19950579906948 22.311391108835508 86-87 21.055489133958766 28.169233316130658 28.398810383017732 22.376467166892848 88-89 21.357697303082972 27.979659470517948 28.274049782034204 22.388593444364876 90-91 21.24032240396609 27.987166032388807 28.372997784444472 22.399513779200632 92-93 21.335669309952838 27.939650246630933 28.282078364288676 22.442602079127557 94-95 21.03565931355574 27.904660205427273 28.339482912391645 22.720197568625345 96-97 21.036364195308213 27.791095364007678 28.38736555254771 22.785174888136396 98-99 20.934177863762045 27.837101034461437 28.292175725933976 22.93654537584254 100-101 20.941510628399914 27.824214740834748 28.205159011237512 23.029115619527822 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 196.0 1 145.5 2 107.5 3 118.0 4 105.5 5 126.0 6 210.0 7 419.0 8 723.0 9 965.0 10 1005.5 11 1005.5 12 1168.0 13 1363.5 14 1562.5 15 1745.0 16 1975.5 17 2227.5 18 2517.5 19 2931.0 20 3446.0 21 4030.0 22 4921.0 23 6440.5 24 8476.0 25 11245.0 26 15680.0 27 21490.0 28 27524.0 29 35168.0 30 43184.5 31 50859.5 32 60728.0 33 71160.0 34 81016.5 35 89802.0 36 100265.0 37 109834.5 38 111983.0 39 111669.5 40 111093.0 41 109390.0 42 108163.5 43 108622.5 44 109696.5 45 110543.5 46 108797.5 47 104783.0 48 101067.0 49 97014.0 50 92073.0 51 86601.5 52 80795.5 53 74265.0 54 68524.0 55 62074.5 56 54772.5 57 47473.0 58 41101.0 59 36220.0 60 30376.5 61 23928.5 62 18320.5 63 13654.5 64 10193.5 65 7462.5 66 5493.0 67 4278.5 68 3351.0 69 2534.0 70 1901.0 71 1403.5 72 1048.0 73 853.0 74 697.5 75 534.5 76 382.0 77 284.5 78 214.0 79 161.0 80 110.0 81 81.0 82 65.0 83 48.5 84 39.0 85 26.0 86 19.5 87 17.0 88 11.5 89 8.0 90 6.0 91 2.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17724237576867752 2 0.0 3 0.005158968672692766 4 2.1201241120655202E-4 5 3.533540186775867E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.02257932179349779 26-27 0.009681900111765876 28-29 0.006908071065146821 30-31 0.0027561613456851763 32-33 0.0013957483737764675 34-35 0.005706667401643026 36-37 0.001837440897123451 38-39 0.0010777297569666395 40-41 0.002844499850354573 42-43 0.0019257794017928475 44-45 7.950465420245701E-4 46-47 0.03810923091437773 48-49 0.020618206989837184 50-51 0.0883208369684628 52-53 0.001943447102726727 54-55 0.03701383345647721 56-57 0.0011837359625699155 58-59 0.0014487514765781056 60-61 0.004134242018527764 62-63 0.004098906616660006 64-65 0.010247266541650014 66-67 0.0059363475137834566 68-69 0.0021731272148671583 70-71 0.005459319588568715 72-73 0.004452260635337593 74-75 8.657173457600875E-4 76-77 0.0 78-79 0.0 80-81 3.003509158759487E-4 82-83 5.653664298841387E-4 84-85 0.0 86-87 0.0012897421681731915 88-89 0.0037632202989162982 90-91 0.0011660682616360363 92-93 0.0030388445606272458 94-95 6.713726354874148E-4 96-97 4.946956261486214E-4 98-99 0.0016960992896524162 100-101 0.002879835252222332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2830023.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.47328163576289 #Duplication Level Percentage of deduplicated Percentage of total 1 81.0609738194451 52.26266994730287 2 11.98105194871414 15.44915473164305 3 3.409432706683431 6.594519453485467 4 1.2705671863349663 3.2767054416693253 5 0.6529607862442451 2.10492623343172 6 0.3536260396670224 1.3679658749514836 7 0.23914390132088206 1.0792874470935425 8 0.1587074181781277 0.8185910455906579 9 0.11500760023015466 0.6673425659902792 >10 0.6506027981097656 7.941956090913252 >50 0.06933486903372524 3.0989121178347347 >100 0.037211121467554495 4.218240667549487 >500 0.0010486514738864627 0.4569368596086594 >1k 2.207687313089227E-4 0.24912506453735433 >5k 1.1038436565446135E-4 0.4136664583983351 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6478 0.22890273329934066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5147 0.18187131341335389 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.11353264620110862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.5335401867758674E-5 0.0 0.0 0.0 0.0 7 3.5335401867758674E-5 0.0 0.0 3.5335401867758674E-5 3.5335401867758674E-5 8 3.5335401867758674E-5 0.0 0.0 3.5335401867758674E-5 3.5335401867758674E-5 9 3.5335401867758674E-5 0.0 0.0 3.5335401867758674E-5 3.5335401867758674E-5 10-11 3.5335401867758674E-5 0.0 0.0 7.067080373551735E-5 3.5335401867758674E-5 12-13 3.5335401867758674E-5 0.0 0.0 1.0600620560327601E-4 3.5335401867758674E-5 14-15 3.5335401867758674E-5 0.0 0.0 1.2367390653715535E-4 3.5335401867758674E-5 16-17 5.300310280163801E-5 0.0 0.0 1.5900930840491401E-4 3.5335401867758674E-5 18-19 1.0600620560327601E-4 0.0 0.0 2.1201241120655202E-4 3.5335401867758674E-5 20-21 1.0600620560327601E-4 0.0 0.0 2.1201241120655202E-4 3.5335401867758674E-5 22-23 1.0600620560327601E-4 0.0 0.0 2.473478130743107E-4 3.5335401867758674E-5 24-25 1.0600620560327601E-4 0.0 0.0 3.886894205453454E-4 3.5335401867758674E-5 26-27 1.0600620560327601E-4 0.0 0.0 9.893912522972428E-4 3.5335401867758674E-5 28-29 1.0600620560327601E-4 0.0 0.0 0.002579484336346383 3.5335401867758674E-5 30-31 1.0600620560327601E-4 0.0 0.0 0.009010527476278462 3.5335401867758674E-5 32-33 1.0600620560327601E-4 0.0 0.0 0.024964461419571503 3.5335401867758674E-5 34-35 1.0600620560327601E-4 0.0 0.0 0.05337412452124947 3.5335401867758674E-5 36-37 1.413416074710347E-4 0.0 0.0 0.09639497629524565 3.5335401867758674E-5 38-39 1.413416074710347E-4 0.0 0.0 0.1561294731526917 3.5335401867758674E-5 40-41 1.413416074710347E-4 0.0 0.0 0.21812543572967427 3.5335401867758674E-5 42-43 1.5900930840491401E-4 0.0 0.0 0.2819588392037803 3.5335401867758674E-5 44-45 1.7667700933879336E-4 0.0 0.0 0.36217020144359247 3.5335401867758674E-5 46-47 1.7667700933879336E-4 0.0 0.0 0.4594308950845982 3.5335401867758674E-5 48-49 1.7667700933879336E-4 0.0 0.0 0.5601367904077105 3.5335401867758674E-5 50-51 1.943447102726727E-4 0.0 0.0 0.6628038005344833 3.5335401867758674E-5 52-53 2.1201241120655202E-4 0.0 0.0 0.7653294690537851 3.5335401867758674E-5 54-55 2.1201241120655202E-4 0.0 0.0 0.8750988949559774 3.5335401867758674E-5 56-57 2.1201241120655202E-4 0.0 0.0 1.0076066519600724 3.5335401867758674E-5 58-59 2.1201241120655202E-4 0.0 0.0 1.1521107778982715 3.5335401867758674E-5 60-61 2.1201241120655202E-4 0.0 0.0 1.3010318290699403 3.5335401867758674E-5 62-63 2.826832149420694E-4 0.0 0.0 1.44868080577437 3.5335401867758674E-5 64-65 2.826832149420694E-4 0.0 0.0 1.6023191330953845 3.5335401867758674E-5 66-67 3.1801861680982803E-4 0.0 0.0 1.7764166580978316 3.5335401867758674E-5 68-69 3.1801861680982803E-4 0.0 0.0 1.973906219136735 3.5335401867758674E-5 70-71 3.1801861680982803E-4 0.0 0.0 2.186271984361965 3.5335401867758674E-5 72-73 3.533540186775867E-4 0.0 0.0 2.406411537998101 3.5335401867758674E-5 74-75 3.533540186775867E-4 0.0 0.0 2.630738336755567 3.5335401867758674E-5 76-77 3.533540186775867E-4 0.0 0.0 2.88041828635315 3.5335401867758674E-5 78-79 3.533540186775867E-4 0.0 0.0 3.1526422223423625 3.5335401867758674E-5 80-81 3.533540186775867E-4 0.0 0.0 3.4437175952280246 3.5335401867758674E-5 82-83 3.533540186775867E-4 0.0 0.0 3.752584342954103 3.5335401867758674E-5 84-85 3.533540186775867E-4 0.0 0.0 4.051857529073086 3.5335401867758674E-5 86-87 3.533540186775867E-4 0.0 0.0 4.370565186219334 3.5335401867758674E-5 88-89 3.533540186775867E-4 0.0 0.0 4.716180751887882 3.5335401867758674E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3595 0.0 55.450325 1 GTATCAA 6750 0.0 42.430847 1 ATCAACG 8155 0.0 34.41608 3 CAACGCA 8295 0.0 33.947918 5 TCAACGC 8500 0.0 33.01744 4 AACGCAG 8785 0.0 31.838192 6 TATCAAC 8960 0.0 31.589016 2 ACGCAGA 9900 0.0 28.060509 7 CGCAGAG 9985 0.0 27.155817 8 GCAGAGT 11395 0.0 23.295515 9 GAGTACT 7305 0.0 21.062 12-13 AGAGTAC 10480 0.0 20.616978 10-11 CAGAGTA 11145 0.0 20.345491 10-11 GTACATG 7595 0.0 20.107878 1 TACATGG 7470 0.0 20.025705 2 TGGTATC 1670 0.0 19.90579 2 GTACTTT 7870 0.0 19.640436 14-15 GTGGTAT 1805 0.0 19.504889 1 ACATGGG 7360 0.0 19.486193 3 GTACTAG 965 0.0 19.227654 1 >>END_MODULE